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Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds

Objetivo

CHASSY will unlock the full potential of the yeasts Saccharomyces cerevisiae, Yarrowia lipolytica and Kluyveromyces marxianus as cell factories for production of high value compounds which have applications in the cosmetic, nutraceutical and white biotechnology sectors. Current cell factory strains for these classes of product are restricted to proof-of-principle levels because of limited precursor supply, poor product tolerance and lack of versatility. CHASSY addresses these challenges by redesigning metabolic circuits and expanding the host range to include the oleaginous yeast, Y. lipolytica and the thermotolerant yeast, K. marxianus. The systems biology approach will integrate model-based design, construction and analysis of yeast strains, resulting in reconfigured metabolic networks optimised for the production of lipid and aromatic molecules. Construction of the chassis strains, using new and existing synthetic biology tools, will be directed by knowledge derived from a thorough systems biology comparison of the three yeast species, conducted using integrative data analysis and genome scale metabolic models. The chassis strains will be used to build cell factories to produce three specific high value products: the oleochemicals, Docosanol and Octanoic acid; and the aromatic molecule, Amorfrutin 1. These new cell factory strains will be evaluated under industrial conditions to produce data that will further improve the chassis platforms. The major outcomes of this project will be (1) a new set of chassis yeast strains that are widely applicable for development of industrial cell factories; (2) the knowledge and technology to readily build and evaluate new chassis tailored to specific applications; (3) prototype cell factory strains producing three high value metabolites for commercial exploitation; (4) a dissemination and exploitation strategy to ensure that European SMEs benefit from the knowledge base, platform chassis and resources generated in CHASSY.

Convocatoria de propuestas

H2020-NMBP-BIO-2016
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Coordinador

UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK
Dirección
Western Road
T12 YN60 Cork
Irlanda
Tipo de actividad
Higher or Secondary Education Establishments
Aportación de la UE
€ 1 228 732,49

Participantes (9)

TECHNISCHE UNIVERSITEIT DELFT
Países Bajos
Aportación de la UE
€ 975 091,25
Dirección
Stevinweg 1
2628 CN Delft
Tipo de actividad
Higher or Secondary Education Establishments
CHALMERS TEKNISKA HOEGSKOLA AB
Suecia
Aportación de la UE
€ 652 135
Dirección
-
41296 Goeteborg
Tipo de actividad
Higher or Secondary Education Establishments
INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
Francia
Aportación de la UE
€ 747 595
Dirección
147 Rue De L'universite
75007 Paris Cedex 07
Tipo de actividad
Research Organisations
JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN
Alemania
Aportación de la UE
€ 535 500
Dirección
Theodor W Adorno Platz 1
60323 Frankfurt Am Main
Tipo de actividad
Higher or Secondary Education Establishments
EVOLVA AG

La participación finalizó

Suiza
Aportación de la UE
€ 0
Dirección
Duggingerstrasse 23
4153 Reinach
Tipo de actividad
Private for-profit entities (excluding Higher or Secondary Education Establishments)
BIOPETROLIA AB
Suecia
Aportación de la UE
€ 702 200
Dirección
Chalmers Tekniska Hogskola
412 96 Goteborg
Tipo de actividad
Private for-profit entities (excluding Higher or Secondary Education Establishments)
NOVA-INSTITUT FUR POLITISCHE UND OKOLOGISCHE INNOVATION GMBH
Alemania
Aportación de la UE
€ 251 000
Dirección
Chemiepark Knapsack Industriestrasse
50354 Huerth
Tipo de actividad
Private for-profit entities (excluding Higher or Secondary Education Establishments)
UCC ACADEMY DESIGNATED ACTIVITY COMPANY
Irlanda
Aportación de la UE
€ 212 500
Dirección
University College Cork National University Of Ireland Western Road
T12 Cork
Tipo de actividad
Other
CLARIANT PRODUKTE (DEUTSCHLAND) GMBH
Alemania
Aportación de la UE
€ 329 856,26
Dirección
Bruningstrasse 50
65929 Frankfurt Am Main
Tipo de actividad
Private for-profit entities (excluding Higher or Secondary Education Establishments)