Understanding the complexity and architecture in protein ubiquitination

Project Information

UbiArchitect

Grant agreement ID: 724804

DOI

10.3030/724804

Project terminated on 31 October 2018

EC signature date 22 March 2017

Start date 1 October 2017

End date 30 September 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 990 125,00

EU contribution

€ 1 990 125,00

Coordinated by

UNITED KINGDOM RESEARCH AND INNOVATION
United Kingdom

Periodic Reporting for period 1 - UbiArchitect (Understanding the complexity and architecture in protein ubiquitination)

Reporting period: 2017-10-01 to 2019-03-31

Summary of the context and overall objectives of the project

The project 'UbiArchitect' set out to further understand architecture in ubqiutiin chains, and to reveal and understand new ubiquitin signals, using a variety of biochemical, mass-spectrometric, biophysical and structural biology techniques.

the ubiquitin system presents a large and mostly untapped translational resource, but the complexity of ubiquitin signals has been daunting for pharmaceutical efforts.

the overall objectives are to develop new methods and techniques to study ubiquitin signals by mass-spectrometry, and apply them to medically relevant pathways. a particular focus is on Parkinson;s disease.

Work performed from the beginning of the project to the end of the period covered by the report and main results achieved so far

The 5 year project was terminated after year 1 as the principal investigator moved to Australia.

During the first year, the group addressed AIM 2a, mechanistic understanding of PINK1/Parkin mediated mitophagy, providing two Nature papers revealing the first structure of PINK1 in complex withubiquitin and a nano body, and the structure of the active form of Parkin. Significant progress was also made on AIM 1, which will be published in 2019.

Progress beyond the state of the art and expected potential impact (including the socio-economic impact and the wider societal implications of the project so far)

the project was terminated, and will be continued in Australia. We expect to address all aims in the next few years.