Development of New therapies against cholangiopathies.

Información del proyecto

New Chol

Identificador del acuerdo de subvención: 741707

DOI

10.3030/741707

Proyecto cerrado

Fecha de la firma de la CE 29 Noviembre 2017

Fecha de inicio 1 Diciembre 2017

Fecha de finalización 30 Noviembre 2023

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 2 499 111,00

Aportación de la UE

€ 2 499 111,00

Coordinado por

CHARITE - UNIVERSITAETSMEDIZIN BERLIN
Germany

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (35)

FXR inhibition may protect from SARS-CoV-2 infection by reducing ACE2

Autores: 109. Brevini T, Maes M, Webb GJ, John BV, Fuchs CD, Buescher G, Wang L, Griffiths C, Brown ML, Scott WE 3rd, Pereyra-Gerber P, Gelson WTH, Brown S, Dillon S, Muraro D, Sharp J, Neary M, Box H, Tatham L, Stewart J, Curley P, Pertinez H, Forrest S, Mlcochova P, Varankar SS, Darvish-Damavandi M, Mulcahy VL, Kuc RE, Williams TL, Heslop JA, Rossetti D, Tysoe OC, Galanakis V, Vila-Gonzalez M, Crozier TW
Publicado en: Nature, 2022, ISSN 1476-4687
Editor: springer
DOI: 10.1038/s41586-022-05594-0

Modeling HNF1B-associated monogenic diabetes using human iPSCs reveals an early stage impairment of the pancreatic developmental program.

Autores: El-Khairi R, Olszanowski E, Muraro D, Madrigal P, Tilgner K, Chhatriwala M, Vyas S, Chia CY, Vallier L*, Rodríguez-Seguí SA
Publicado en: Stem Cell Reports, 2021, ISSN 2213-6711
Editor: Cell Press
DOI: 10.1016/j.stemcr.2021.07.018

Single-cell atlas of human liver development reveals pathways directing hepatic cell fates.

Autores: Wesley BT, Ross ADB, Muraro D, Miao Z, Saxton S, Tomaz RA, Morell CM, Ridley K, Zacharis ED, Petrus-Reurer S, Kraiczy J, Mahbubani KT, Brown S, Garcia-Bernardo J, Alsinet C, Gaffney D, Horsfall D, Tysoe OC, Botting RA, Stephenson E, Popescu DM, MacParland S, Bader G, McGilvray ID, Ortmann D, Sampaziotis F, Saeb-Parsy K, Haniffa M, Stevens KR, Zilbauer M, Teichmann SA, Vallier L.
Publicado en: Nature Cell Biology, 2022, ISSN 1465-7392
Editor: Nature Publishing Group
DOI: 10.1038/s41556-022-00989-7

Unreveling the developmental roadmap toward human brown adipose tissue.

Autores: Stefania Carobbio 1, Anne-Claire Guenantin 2, Myriam Bahri 3, Sonia Rodriguez-Fdez 3, Floris Honig 3, Ioannis Kamzolas 4, Isabella Samuelson 2, Kathleen Long 3, Sherine Awad 5, Dunja Lukovic 6, Slaven Erceg 7, Andrew Bassett 3, Sasha Mendjan 8, Ludovic Vallier 9, Barry S Rosen 3, Davide Chiarugi 5, Antonio Vidal-Puig 10
Publicado en: Stem Cell Reports, 2021, ISSN 2213-6711
Editor: Cell Press
DOI: 10.1016/j.stemcr.2021.01.013

Cholangiocyte organoids can repair bile ducts after transplantation in the human liver

Autores: Sampaziotis F, Muraro D, Tysoe OC, Sawiak S, Beach TE, Godfrey EM, Upponi SS, Brevini T, Wesley BT, Garcia-Bernardo J, Mahbubani K, Canu G, Gieseck R 3rd, Berntsen NL, Mulcahy VL, Crick K, Fear C, Robinson S, Swift L, Gambardella L, Bargehr J, Ortmann D, Brown SE, Osnato A, Murphy MP, Corbett G, Gelson WTH, Mells GF, Humphreys P, Davies SE, Amin I, Gibbs P, Sinha S, Teichmann SA, Butler AJ, See TC
Publicado en: Science, 2021, ISSN 0036-8075
Editor: American Association for the Advancement of Science
DOI: 10.1126/science.aaz6964

Modeling Nonalcoholic Fatty Liver Disease in the Dish Using Human-Specific Platforms: Strategies and Limitations

Autores: Rezvani M, Vallier L, Guillot A
Publicado en: Cell Mol Gastroenterol Hepatol, 2023, ISSN 2352-345X
Editor: Elsevier Inc.
DOI: 10.1016/j.jcmgh.2023.01.014

Modeling: In Vitro Pancreatic Differentiation From Human Pluripotent Stem Cells Gains Momentum

Autores: Burgos JI, Vallier L, Rodríguez-Seguí SA.
Publicado en: Frontiers in Endocrinology, 2021, ISSN 1664-2392
Editor: Frontiers
DOI: 10.3389/fendo.2021.692596

Single-cell transcriptomic characterization of a gastrulating human embryo

Autores: Tyser RCV, Mahammadov E, Nakanoh S, Vallier L, Scialdone A, Srinivas S
Publicado en: Nature, 2021, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/s41586-021-04158-y

Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications.

Autores: Vitillo L, Vallier L
Publicado en: Methods Mol Biol, 2021, ISSN 1064-3745
Editor: Humana Press, Inc.
DOI: 10.1007/7651_2021_401

Organoids capture tissue-specific innate lymphoid cell development in mice and humans.

Autores: Jowett GM, Read E, Roberts LB, Coman D, Vilà González M, Zabinski T, Niazi U, Reis R, Trieu TJ, Danovi D, Gentleman E, Vallier L, Curtis MA, Lord GM, Neves JF.
Publicado en: Cell Reports, 2022, ISSN 2211-1247
Editor: Cell Press
DOI: 10.1016/j.celrep.2022.111281

PNPLA3-Associated NAFLD Using Human-Induced Pluripotent Stem Cells.

Autores: Tilson SG, Morell CM, Lenaerts AS, Park SB, Hu Z, Jenkins B, Koulman A, Liang TJ*, Vallier L
Publicado en: Heptology, 2021, ISSN 2471-254X
Editor: American Association for the Study of Liver Diseases
DOI: 10.1002/hep.32063

GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells.

Autores: 87. Vitillo L, Durance C, Hewitt Z, Moore H, Smith A, Vallier L.
Publicado en: Stem Cell Research Therapy, 2020, ISSN 1757-6512
Editor: BioMed Central
DOI: 10.1186/s13287-020-01915-0

Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids.

Autores: Marsee A, Roos FJM, Verstegen MMA; HPB Organoid Consortium, Gehart H, de Koning E, Lemaigre F, Forbes SJ, Peng WC, Huch M, Takebe T, Vallier L, Clevers H, van der Laan LJW, Spee B
Publicado en: Cell Stem Cell, 2021, ISSN 1934-5909
Editor: Cell Press
DOI: 10.1016/j.stem.2021.04.005

hiPSC hepatocyte model demonstrates the role of unfolded protein response and inflammatory networks in α1-antitrypsin deficiency

Autores: Charis-Patricia Segeritz, Sheikh Tamir Rashid, Miguel Cardoso de Brito, Maria Paola Serra, Adriana Ordonez, Carola Maria Morell, Joseph E. Kaserman, Pedro Madrigal, Nicholas R.F. Hannan, Laurent Gatto, Lu Tan, Andrew A. Wilson, Kathryn Lilley, Stefan J. Marciniak, Bibek Gooptu, David A. Lomas, Ludovic Vallier
Publicado en: Journal of Hepatology, Edición 69/4, 2018, Página(s) 851-860, ISSN 0168-8278
Editor: Elsevier BV
DOI: 10.1016/j.jhep.2018.05.028

HNF4A Haploinsufficiency in MODY1 Abrogates Liver and Pancreas Differentiation from Patient-Derived Induced Pluripotent Stem Cells

Autores: Natasha Hui Jin Ng, Joanita Binte Jasmen, Chang Siang Lim, Hwee Hui Lau, Vidhya Gomathi Krishnan, Juned Kadiwala, Rohit N. Kulkarni, Helge Ræder, Ludovic Vallier, Shawn Hoon, Adrian Kee Keong Teo
Publicado en: iScience, Edición 16, 2019, Página(s) 192-205, ISSN 2589-0042
Editor: Elsevier
DOI: 10.1016/j.isci.2019.05.032

Combined single-cell profiling of expression and DNA methylation reveals splicing regulation and heterogeneity

Autores: Stephanie M. Linker, Lara Urban, Stephen J. Clark, Mariya Chhatriwala, Shradha Amatya, Davis J. McCarthy, Ingo Ebersberger, Ludovic Vallier, Wolf Reik, Oliver Stegle, Marc Jan Bonder
Publicado en: Genome Biology, Edición 20/1, 2019, ISSN 1474-760X
Editor: BMC
DOI: 10.1186/s13059-019-1644-0

Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue

Autores: Olivia C. Tysoe, Alexander W. Justin, Teresa Brevini, Si Emma Chen, Krishnaa T. Mahbubani, Anna K. Frank, Hajer Zedira, Espen Melum, Kourosh Saeb-Parsy, Athina E. Markaki, Ludovic Vallier, Fotios Sampaziotis
Publicado en: Nature Protocols, Edición 14/6, 2019, Página(s) 1884-1925, ISSN 1754-2189
Editor: Nature Publishing Group
DOI: 10.1038/s41596-019-0168-0

Genome-Wide Epigenetic and Transcriptomic Characterization of Human-Induced Pluripotent Stem Cell–Derived Intestinal Epithelial Organoids

Autores: Judith Kraiczy, Alexander D.B. Ross, Jessica L. Forbester, Gordon Dougan, Ludovic Vallier, Matthias Zilbauer
Publicado en: Cellular and Molecular Gastroenterology and Hepatology, Edición 7/2, 2019, Página(s) 285-288, ISSN 2352-345X
Editor: Elsevier Inc.
DOI: 10.1016/j.jcmgh.2018.10.008

Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics

Autores: Loukia Yiangou, Rodrigo A. Grandy, Carola M. Morell, Rute A. Tomaz, Anna Osnato, Juned Kadiwala, Daniele Muraro, Jose Garcia-Bernardo, Shota Nakanoh, William G. Bernard, Daniel Ortmann, Davis J. McCarthy, Ingrid Simonic, Sanjay Sinha, Ludovic Vallier
Publicado en: Stem Cell Reports, Edición 12/1, 2019, Página(s) 165-179, ISSN 2213-6711
Editor: Cell Press
DOI: 10.1016/j.stemcr.2018.11.020

Human Pluripotent Stem Cell-Derived Endoderm for Modeling Development and Clinical Applications

Autores: Loukia Yiangou, Alexander D.B. Ross, Kim Jee Goh, Ludovic Vallier
Publicado en: Cell Stem Cell, Edición 22/4, 2018, Página(s) 485-499, ISSN 1934-5909
Editor: Cell Press
DOI: 10.1016/j.stem.2018.03.016

Regenerative cell therapy for the treatment of hyperbilirubinemic Gunn rats with fresh and frozen human induced pluripotent stem cells‐derived hepatic stem cells

Autores: Angélique Fourrier, Frédéric Delbos, Séverine Menoret, Camille Collet, Linh Trinh Thi Thuy, Anne Myara, François Petit, Laia Tolosa, Sophie Laplanche, María José Gómez‐Lechón, Philippe Labrune, Ignacio Anegon, Ludovic Vallier, Delphine Garnier, Tuan Huy Nguyen
Publicado en: Xenotransplantation, 2019, ISSN 0908-665X
Editor: Blackwell Publishing Inc.
DOI: 10.1111/xen.12544

Advances in the generation of bioengineered bile ducts

Autores: Alexander W. Justin, Kourosh Saeb-Parsy, Athina E. Markaki, Ludovic Vallier, Fotios Sampaziotis
Publicado en: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Edición 1864/4, 2018, Página(s) 1532-1538, ISSN 0925-4439
Editor: Elsevier BV
DOI: 10.1016/j.bbadis.2017.10.034

The SMAD2/3 interactome reveals that TGFβ controls m6A mRNA methylation in pluripotency

Autores: Alessandro Bertero, Stephanie Brown, Pedro Madrigal, Anna Osnato, Daniel Ortmann, Loukia Yiangou, Juned Kadiwala, Nina C. Hubner, Igor Ruiz de los Mozos, Christoph Sadée, An-Sofie Lenaerts, Shota Nakanoh, Rodrigo Grandy, Edward Farnell, Jernej Ule, Hendrik G. Stunnenberg, Sasha Mendjan, Ludovic Vallier
Publicado en: Nature, Edición 555/7695, 2018, Página(s) 256-259, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/nature25784

Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression

Autores: Anna S. E. Cuomo, Daniel D. Seaton, Davis J. McCarthy, Iker Martinez, Marc Jan Bonder, Jose Garcia-Bernardo, Shradha Amatya, Pedro Madrigal, Abigail Isaacson, Florian Buettner, Andrew Knights, Kedar Nath Natarajan, Ludovic Vallier, John C. Marioni, Mariya Chhatriwala, Oliver Stegle
Publicado en: Nature Communications, Edición 11/1, 2020, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-020-14457-z

A Novel Chemically Differentiated Mouse Embryonic Stem Cell-Based Model to Study Liver Stages of Plasmodium berghei

Autores: Jaishree Tripathi, Charis-Patricia Segeritz, Gareth Griffiths, Wendy Bushell, Ludovic Vallier, William C. Skarnes, Maria M. Mota, Oliver Billker
Publicado en: Stem Cell Reports, Edición 14/6, 2020, Página(s) 1123-1134, ISSN 2213-6711
Editor: Cell Press
DOI: 10.1016/j.stemcr.2020.04.010

GDF15 mediates the effects of metformin on body weight and energy balance

Autores: Anthony P. Coll, Michael Chen, Pranali Taskar, Debra Rimmington, Satish Patel, John A. Tadross, Irene Cimino, Ming Yang, Paul Welsh, Samuel Virtue, Deborah A. Goldspink, Emily L. Miedzybrodzka, Adam R. Konopka, Raul Ruiz Esponda, Jeffrey T.-J. Huang, Y. C. Loraine Tung, Sergio Rodriguez-Cuenca, Rute A. Tomaz, Heather P. Harding, Audrey Melvin, Giles S. H. Yeo, David Preiss, Antonio Vidal-Puig, Lud
Publicado en: Nature, Edición 578/7795, 2020, Página(s) 444-448, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/s41586-019-1911-y

SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes

Autores: Waradon Sungnak, Ni Huang, Christophe Bécavin, Marijn Berg, Rachel Queen, Monika Litvinukova, Carlos Talavera-López, Henrike Maatz, Daniel Reichart, Fotios Sampaziotis, Kaylee B. Worlock, Masahiro Yoshida, Josephine L. Barnes
Publicado en: Nature Medicine, Edición 26/5, 2020, Página(s) 681-687, ISSN 1078-8956
Editor: Nature Publishing Group
DOI: 10.1038/s41591-020-0868-6

Regional differences in human biliary tissues and corresponding in vitro derived organoids

Autores: Casey A Rimland, Samantha G Tilson, Carola M Morell, Rute A Tomaz, Wei-Yu Lu, Simone E Adams, Nikitas Georgakopoulos, Francisco Otaizo-Carrasquero, Timothy G Myers, John R Ferdinand, Richard L Gieseck, Fotios Sampaziotis, Olivia C Tysoe, Brandon Wesley, Daniele Muraro, Gabriel C Oniscu, Nicholas RF Hannan, Stuart J Forbes, Kourosh Saeb-Parsy, Thomas A Wynn, Ludovic Vallier
Publicado en: Hepatology, 2020, ISSN 0270-9139
Editor: John Wiley & Sons Inc.
DOI: 10.1002/hep.31252

Analysis of endothelial-to-haematopoietic transition at the single cell level identifies cell cycle regulation as a driver of differentiation

Autores: Giovanni Canu, Emmanouil Athanasiadis, Rodrigo A. Grandy, Jose Garcia-Bernardo, Paulina M. Strzelecka, Ludovic Vallier, Daniel Ortmann, Ana Cvejic
Publicado en: Genome Biology, Edición 21/1, 2020, ISSN 1474-760X
Editor: Springer Nature
DOI: 10.1186/s13059-020-02058-4

Cell cycle regulators control mesoderm specification in human pluripotent stem cells

Autores: Loukia Yiangou, Rodrigo A. Grandy, Anna Osnato, Daniel Ortmann, Sanjay Sinha, Ludovic Vallier
Publicado en: Journal of Biological Chemistry, Edición 294/47, 2019, Página(s) 17903-17914, ISSN 0021-9258
Editor: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra119.008251

Human branching cholangiocyte organoids recapitulate functional bile duct formation.

Autores: 107. Roos FJM, van Tienderen GS, Wu H, Bordeu I, Vinke D, Albarinos LM, Monfils K, Niesten S, Smits R, Willemse J, Rosmark O, Westergren-Thorsson G, Kunz DJ, de Wit M, French PJ, Vallier L, IJzermans JNM, Bartfai R, Marks H, Simons BD, van Royen ME, Verstegen MMA, van der Laan LJW
Publicado en: Cell Stem Cell, 2022, ISSN 1934-5909
Editor: Cell Press
DOI: 10.1016/j.stem.2022.04.011

Naive Pluripotent Stem Cells Exhibit Phenotypic Variability that Is Driven by Genetic Variation.

Autores: Ortmann D, Brown S, Czechanski A, Aydin S, Muraro D, Huang Y, Tomaz RA, Osnato A, Canu G, Wesley BT, Skelly DA, Stegle O, Choi T, Churchill GA, Baker CL, Rugg-Gunn PJ, Munger SC, Reinholdt LG, Vallier L
Publicado en: Cell Stem Cell, 2020, ISSN 1934-5909
Editor: Cell Press
DOI: 10.1016/j.stem.2020.07.019

Single-Cell Sequencing of Developing Human Gut Reveals Transcriptional Links to Childhood Crohn's Disease

Autores: Elmentaite R, Ross ADB, Roberts K, James KR, Ortmann D, Gomes T, Nayak K, Tuck L, Pritchard S, Bayraktar OA, Heuschkel R, Vallier L, Teichmann SA, Zilbauer M
Publicado en: Developmental Cell, 2021, ISSN 1534-5807
Editor: Cell Press
DOI: 10.1016/j.devcel.2020.11.010

TGFβ signalling is required to maintain pluripotency of human naïve pluripotent stem cells.

Autores: Osnato A, Brown S, Krueger C, Andrews S, Collier AJ, Nakanoh S, Quiroga Londoño M, Wesley BT, Muraro D, Brumm AS, Niakan KK, Vallier L*, Ortmann D*, Rugg-Gunn PJ*
Publicado en: Elife, 2021, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.67259

Generation of functional hepatocytes by forward programming with nuclear receptors

Autores: Tomaz RA, Zacharis ED, Bachinger F, Wurmser A, Yamamoto D, Petrus-Reurer S, Morell CM, Dziedzicka D, Wesley BT, Geti I, Segeritz CP, de Brito MC, Chhatriwala M, Ortmann D, Saeb-Parsy K, Vallier L.
Publicado en: elife, 2022, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.71591

Non-peer reviewed articles (1)

Modeling Disease with Human Inducible Pluripotent Stem Cells

Autores: Rodrigo Grandy, Rute A. Tomaz, Ludovic Vallier
Publicado en: Annual Review of Pathology: Mechanisms of Disease, Edición 14/1, 2019, Página(s) 449-468, ISSN 1553-4006
Editor: Annual Reviews, Inc.
DOI: 10.1146/annurev-pathol-020117-043634

Book chapters (1)

Use of Biliary Organoids in Cholestasis Research

Autores: Fotios Sampaziotis, Olivia Tysoe, Teresa Brevini, Ludovic Vallier
Publicado en: Experimental Cholestasis Research, Edición 1981, 2019, Página(s) 373-382, ISBN 978-1-4939-9419-9
Editor: Springer New York
DOI: 10.1007/978-1-4939-9420-5_25

Derechos de propiedad intelectual

Patent (3)

TRANSPLANTATION OF LIVER CELLS BY ADMINISTRATION TO THE BILIARY TREE OF THE LIVER

Número de solicitud/publicación: 22 707076
Fecha: 2022-02-17
Solicitante(s): THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

IN VITRO PRODUCTION OF CHOLANGIOCYTES

Número de solicitud/publicación: 18 733834
Fecha: 2016-06-21
Solicitante(s): THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

METHODS OF EXPANDING CHOLANGIOCYTES

Número de solicitud/publicación: 18 733834
Fecha: 2018-06-19
Solicitante(s): THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE