Development of New therapies against cholangiopathies.

Informations projet

New Chol

N° de convention de subvention: 741707

DOI

10.3030/741707

Projet clôturé

Date de signature de la CE 29 Novembre 2017

Date de début 1 Decembre 2017

Date de fin 30 Novembre 2023

Financé au titre de

• EXCELLENT SCIENCE - European Research Council (ERC)

Coût total

€ 2 499 111,00

Contribution de l’UE

€ 2 499 111,00

Coordonné par

CHARITE - UNIVERSITAETSMEDIZIN BERLIN
Germany

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Publications

Peer reviewed articles (35)

FXR inhibition may protect from SARS-CoV-2 infection by reducing ACE2

Auteurs: 109. Brevini T, Maes M, Webb GJ, John BV, Fuchs CD, Buescher G, Wang L, Griffiths C, Brown ML, Scott WE 3rd, Pereyra-Gerber P, Gelson WTH, Brown S, Dillon S, Muraro D, Sharp J, Neary M, Box H, Tatham L, Stewart J, Curley P, Pertinez H, Forrest S, Mlcochova P, Varankar SS, Darvish-Damavandi M, Mulcahy VL, Kuc RE, Williams TL, Heslop JA, Rossetti D, Tysoe OC, Galanakis V, Vila-Gonzalez M, Crozier TW
Publié dans: Nature, 2022, ISSN 1476-4687
Éditeur: springer
DOI: 10.1038/s41586-022-05594-0

Modeling HNF1B-associated monogenic diabetes using human iPSCs reveals an early stage impairment of the pancreatic developmental program.

Auteurs: El-Khairi R, Olszanowski E, Muraro D, Madrigal P, Tilgner K, Chhatriwala M, Vyas S, Chia CY, Vallier L*, Rodríguez-Seguí SA
Publié dans: Stem Cell Reports, 2021, ISSN 2213-6711
Éditeur: Cell Press
DOI: 10.1016/j.stemcr.2021.07.018

Single-cell atlas of human liver development reveals pathways directing hepatic cell fates.

Auteurs: Wesley BT, Ross ADB, Muraro D, Miao Z, Saxton S, Tomaz RA, Morell CM, Ridley K, Zacharis ED, Petrus-Reurer S, Kraiczy J, Mahbubani KT, Brown S, Garcia-Bernardo J, Alsinet C, Gaffney D, Horsfall D, Tysoe OC, Botting RA, Stephenson E, Popescu DM, MacParland S, Bader G, McGilvray ID, Ortmann D, Sampaziotis F, Saeb-Parsy K, Haniffa M, Stevens KR, Zilbauer M, Teichmann SA, Vallier L.
Publié dans: Nature Cell Biology, 2022, ISSN 1465-7392
Éditeur: Nature Publishing Group
DOI: 10.1038/s41556-022-00989-7

Unreveling the developmental roadmap toward human brown adipose tissue.

Auteurs: Stefania Carobbio 1, Anne-Claire Guenantin 2, Myriam Bahri 3, Sonia Rodriguez-Fdez 3, Floris Honig 3, Ioannis Kamzolas 4, Isabella Samuelson 2, Kathleen Long 3, Sherine Awad 5, Dunja Lukovic 6, Slaven Erceg 7, Andrew Bassett 3, Sasha Mendjan 8, Ludovic Vallier 9, Barry S Rosen 3, Davide Chiarugi 5, Antonio Vidal-Puig 10
Publié dans: Stem Cell Reports, 2021, ISSN 2213-6711
Éditeur: Cell Press
DOI: 10.1016/j.stemcr.2021.01.013

Cholangiocyte organoids can repair bile ducts after transplantation in the human liver

Auteurs: Sampaziotis F, Muraro D, Tysoe OC, Sawiak S, Beach TE, Godfrey EM, Upponi SS, Brevini T, Wesley BT, Garcia-Bernardo J, Mahbubani K, Canu G, Gieseck R 3rd, Berntsen NL, Mulcahy VL, Crick K, Fear C, Robinson S, Swift L, Gambardella L, Bargehr J, Ortmann D, Brown SE, Osnato A, Murphy MP, Corbett G, Gelson WTH, Mells GF, Humphreys P, Davies SE, Amin I, Gibbs P, Sinha S, Teichmann SA, Butler AJ, See TC
Publié dans: Science, 2021, ISSN 0036-8075
Éditeur: American Association for the Advancement of Science
DOI: 10.1126/science.aaz6964

Modeling Nonalcoholic Fatty Liver Disease in the Dish Using Human-Specific Platforms: Strategies and Limitations

Auteurs: Rezvani M, Vallier L, Guillot A
Publié dans: Cell Mol Gastroenterol Hepatol, 2023, ISSN 2352-345X
Éditeur: Elsevier Inc.
DOI: 10.1016/j.jcmgh.2023.01.014

Modeling: In Vitro Pancreatic Differentiation From Human Pluripotent Stem Cells Gains Momentum

Auteurs: Burgos JI, Vallier L, Rodríguez-Seguí SA.
Publié dans: Frontiers in Endocrinology, 2021, ISSN 1664-2392
Éditeur: Frontiers
DOI: 10.3389/fendo.2021.692596

Single-cell transcriptomic characterization of a gastrulating human embryo

Auteurs: Tyser RCV, Mahammadov E, Nakanoh S, Vallier L, Scialdone A, Srinivas S
Publié dans: Nature, 2021, ISSN 0028-0836
Éditeur: Nature Publishing Group
DOI: 10.1038/s41586-021-04158-y

Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications.

Auteurs: Vitillo L, Vallier L
Publié dans: Methods Mol Biol, 2021, ISSN 1064-3745
Éditeur: Humana Press, Inc.
DOI: 10.1007/7651_2021_401

Organoids capture tissue-specific innate lymphoid cell development in mice and humans.

Auteurs: Jowett GM, Read E, Roberts LB, Coman D, Vilà González M, Zabinski T, Niazi U, Reis R, Trieu TJ, Danovi D, Gentleman E, Vallier L, Curtis MA, Lord GM, Neves JF.
Publié dans: Cell Reports, 2022, ISSN 2211-1247
Éditeur: Cell Press
DOI: 10.1016/j.celrep.2022.111281

PNPLA3-Associated NAFLD Using Human-Induced Pluripotent Stem Cells.

Auteurs: Tilson SG, Morell CM, Lenaerts AS, Park SB, Hu Z, Jenkins B, Koulman A, Liang TJ*, Vallier L
Publié dans: Heptology, 2021, ISSN 2471-254X
Éditeur: American Association for the Study of Liver Diseases
DOI: 10.1002/hep.32063

GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells.

Auteurs: 87. Vitillo L, Durance C, Hewitt Z, Moore H, Smith A, Vallier L.
Publié dans: Stem Cell Research Therapy, 2020, ISSN 1757-6512
Éditeur: BioMed Central
DOI: 10.1186/s13287-020-01915-0

Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids.

Auteurs: Marsee A, Roos FJM, Verstegen MMA; HPB Organoid Consortium, Gehart H, de Koning E, Lemaigre F, Forbes SJ, Peng WC, Huch M, Takebe T, Vallier L, Clevers H, van der Laan LJW, Spee B
Publié dans: Cell Stem Cell, 2021, ISSN 1934-5909
Éditeur: Cell Press
DOI: 10.1016/j.stem.2021.04.005

hiPSC hepatocyte model demonstrates the role of unfolded protein response and inflammatory networks in α1-antitrypsin deficiency

Auteurs: Charis-Patricia Segeritz, Sheikh Tamir Rashid, Miguel Cardoso de Brito, Maria Paola Serra, Adriana Ordonez, Carola Maria Morell, Joseph E. Kaserman, Pedro Madrigal, Nicholas R.F. Hannan, Laurent Gatto, Lu Tan, Andrew A. Wilson, Kathryn Lilley, Stefan J. Marciniak, Bibek Gooptu, David A. Lomas, Ludovic Vallier
Publié dans: Journal of Hepatology, Numéro 69/4, 2018, Page(s) 851-860, ISSN 0168-8278
Éditeur: Elsevier BV
DOI: 10.1016/j.jhep.2018.05.028

HNF4A Haploinsufficiency in MODY1 Abrogates Liver and Pancreas Differentiation from Patient-Derived Induced Pluripotent Stem Cells

Auteurs: Natasha Hui Jin Ng, Joanita Binte Jasmen, Chang Siang Lim, Hwee Hui Lau, Vidhya Gomathi Krishnan, Juned Kadiwala, Rohit N. Kulkarni, Helge Ræder, Ludovic Vallier, Shawn Hoon, Adrian Kee Keong Teo
Publié dans: iScience, Numéro 16, 2019, Page(s) 192-205, ISSN 2589-0042
Éditeur: Elsevier
DOI: 10.1016/j.isci.2019.05.032

Combined single-cell profiling of expression and DNA methylation reveals splicing regulation and heterogeneity

Auteurs: Stephanie M. Linker, Lara Urban, Stephen J. Clark, Mariya Chhatriwala, Shradha Amatya, Davis J. McCarthy, Ingo Ebersberger, Ludovic Vallier, Wolf Reik, Oliver Stegle, Marc Jan Bonder
Publié dans: Genome Biology, Numéro 20/1, 2019, ISSN 1474-760X
Éditeur: BMC
DOI: 10.1186/s13059-019-1644-0

Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue

Auteurs: Olivia C. Tysoe, Alexander W. Justin, Teresa Brevini, Si Emma Chen, Krishnaa T. Mahbubani, Anna K. Frank, Hajer Zedira, Espen Melum, Kourosh Saeb-Parsy, Athina E. Markaki, Ludovic Vallier, Fotios Sampaziotis
Publié dans: Nature Protocols, Numéro 14/6, 2019, Page(s) 1884-1925, ISSN 1754-2189
Éditeur: Nature Publishing Group
DOI: 10.1038/s41596-019-0168-0

Genome-Wide Epigenetic and Transcriptomic Characterization of Human-Induced Pluripotent Stem Cell–Derived Intestinal Epithelial Organoids

Auteurs: Judith Kraiczy, Alexander D.B. Ross, Jessica L. Forbester, Gordon Dougan, Ludovic Vallier, Matthias Zilbauer
Publié dans: Cellular and Molecular Gastroenterology and Hepatology, Numéro 7/2, 2019, Page(s) 285-288, ISSN 2352-345X
Éditeur: Elsevier Inc.
DOI: 10.1016/j.jcmgh.2018.10.008

Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics

Auteurs: Loukia Yiangou, Rodrigo A. Grandy, Carola M. Morell, Rute A. Tomaz, Anna Osnato, Juned Kadiwala, Daniele Muraro, Jose Garcia-Bernardo, Shota Nakanoh, William G. Bernard, Daniel Ortmann, Davis J. McCarthy, Ingrid Simonic, Sanjay Sinha, Ludovic Vallier
Publié dans: Stem Cell Reports, Numéro 12/1, 2019, Page(s) 165-179, ISSN 2213-6711
Éditeur: Cell Press
DOI: 10.1016/j.stemcr.2018.11.020

Human Pluripotent Stem Cell-Derived Endoderm for Modeling Development and Clinical Applications

Auteurs: Loukia Yiangou, Alexander D.B. Ross, Kim Jee Goh, Ludovic Vallier
Publié dans: Cell Stem Cell, Numéro 22/4, 2018, Page(s) 485-499, ISSN 1934-5909
Éditeur: Cell Press
DOI: 10.1016/j.stem.2018.03.016

Regenerative cell therapy for the treatment of hyperbilirubinemic Gunn rats with fresh and frozen human induced pluripotent stem cells‐derived hepatic stem cells

Auteurs: Angélique Fourrier, Frédéric Delbos, Séverine Menoret, Camille Collet, Linh Trinh Thi Thuy, Anne Myara, François Petit, Laia Tolosa, Sophie Laplanche, María José Gómez‐Lechón, Philippe Labrune, Ignacio Anegon, Ludovic Vallier, Delphine Garnier, Tuan Huy Nguyen
Publié dans: Xenotransplantation, 2019, ISSN 0908-665X
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/xen.12544

Advances in the generation of bioengineered bile ducts

Auteurs: Alexander W. Justin, Kourosh Saeb-Parsy, Athina E. Markaki, Ludovic Vallier, Fotios Sampaziotis
Publié dans: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Numéro 1864/4, 2018, Page(s) 1532-1538, ISSN 0925-4439
Éditeur: Elsevier BV
DOI: 10.1016/j.bbadis.2017.10.034

The SMAD2/3 interactome reveals that TGFβ controls m6A mRNA methylation in pluripotency

Auteurs: Alessandro Bertero, Stephanie Brown, Pedro Madrigal, Anna Osnato, Daniel Ortmann, Loukia Yiangou, Juned Kadiwala, Nina C. Hubner, Igor Ruiz de los Mozos, Christoph Sadée, An-Sofie Lenaerts, Shota Nakanoh, Rodrigo Grandy, Edward Farnell, Jernej Ule, Hendrik G. Stunnenberg, Sasha Mendjan, Ludovic Vallier
Publié dans: Nature, Numéro 555/7695, 2018, Page(s) 256-259, ISSN 0028-0836
Éditeur: Nature Publishing Group
DOI: 10.1038/nature25784

Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression

Auteurs: Anna S. E. Cuomo, Daniel D. Seaton, Davis J. McCarthy, Iker Martinez, Marc Jan Bonder, Jose Garcia-Bernardo, Shradha Amatya, Pedro Madrigal, Abigail Isaacson, Florian Buettner, Andrew Knights, Kedar Nath Natarajan, Ludovic Vallier, John C. Marioni, Mariya Chhatriwala, Oliver Stegle
Publié dans: Nature Communications, Numéro 11/1, 2020, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-020-14457-z

A Novel Chemically Differentiated Mouse Embryonic Stem Cell-Based Model to Study Liver Stages of Plasmodium berghei

Auteurs: Jaishree Tripathi, Charis-Patricia Segeritz, Gareth Griffiths, Wendy Bushell, Ludovic Vallier, William C. Skarnes, Maria M. Mota, Oliver Billker
Publié dans: Stem Cell Reports, Numéro 14/6, 2020, Page(s) 1123-1134, ISSN 2213-6711
Éditeur: Cell Press
DOI: 10.1016/j.stemcr.2020.04.010

GDF15 mediates the effects of metformin on body weight and energy balance

Auteurs: Anthony P. Coll, Michael Chen, Pranali Taskar, Debra Rimmington, Satish Patel, John A. Tadross, Irene Cimino, Ming Yang, Paul Welsh, Samuel Virtue, Deborah A. Goldspink, Emily L. Miedzybrodzka, Adam R. Konopka, Raul Ruiz Esponda, Jeffrey T.-J. Huang, Y. C. Loraine Tung, Sergio Rodriguez-Cuenca, Rute A. Tomaz, Heather P. Harding, Audrey Melvin, Giles S. H. Yeo, David Preiss, Antonio Vidal-Puig, Lud
Publié dans: Nature, Numéro 578/7795, 2020, Page(s) 444-448, ISSN 0028-0836
Éditeur: Nature Publishing Group
DOI: 10.1038/s41586-019-1911-y

SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes

Auteurs: Waradon Sungnak, Ni Huang, Christophe Bécavin, Marijn Berg, Rachel Queen, Monika Litvinukova, Carlos Talavera-López, Henrike Maatz, Daniel Reichart, Fotios Sampaziotis, Kaylee B. Worlock, Masahiro Yoshida, Josephine L. Barnes
Publié dans: Nature Medicine, Numéro 26/5, 2020, Page(s) 681-687, ISSN 1078-8956
Éditeur: Nature Publishing Group
DOI: 10.1038/s41591-020-0868-6

Regional differences in human biliary tissues and corresponding in vitro derived organoids

Auteurs: Casey A Rimland, Samantha G Tilson, Carola M Morell, Rute A Tomaz, Wei-Yu Lu, Simone E Adams, Nikitas Georgakopoulos, Francisco Otaizo-Carrasquero, Timothy G Myers, John R Ferdinand, Richard L Gieseck, Fotios Sampaziotis, Olivia C Tysoe, Brandon Wesley, Daniele Muraro, Gabriel C Oniscu, Nicholas RF Hannan, Stuart J Forbes, Kourosh Saeb-Parsy, Thomas A Wynn, Ludovic Vallier
Publié dans: Hepatology, 2020, ISSN 0270-9139
Éditeur: John Wiley & Sons Inc.
DOI: 10.1002/hep.31252

Analysis of endothelial-to-haematopoietic transition at the single cell level identifies cell cycle regulation as a driver of differentiation

Auteurs: Giovanni Canu, Emmanouil Athanasiadis, Rodrigo A. Grandy, Jose Garcia-Bernardo, Paulina M. Strzelecka, Ludovic Vallier, Daniel Ortmann, Ana Cvejic
Publié dans: Genome Biology, Numéro 21/1, 2020, ISSN 1474-760X
Éditeur: Springer Nature
DOI: 10.1186/s13059-020-02058-4

Cell cycle regulators control mesoderm specification in human pluripotent stem cells

Auteurs: Loukia Yiangou, Rodrigo A. Grandy, Anna Osnato, Daniel Ortmann, Sanjay Sinha, Ludovic Vallier
Publié dans: Journal of Biological Chemistry, Numéro 294/47, 2019, Page(s) 17903-17914, ISSN 0021-9258
Éditeur: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra119.008251

Human branching cholangiocyte organoids recapitulate functional bile duct formation.

Auteurs: 107. Roos FJM, van Tienderen GS, Wu H, Bordeu I, Vinke D, Albarinos LM, Monfils K, Niesten S, Smits R, Willemse J, Rosmark O, Westergren-Thorsson G, Kunz DJ, de Wit M, French PJ, Vallier L, IJzermans JNM, Bartfai R, Marks H, Simons BD, van Royen ME, Verstegen MMA, van der Laan LJW
Publié dans: Cell Stem Cell, 2022, ISSN 1934-5909
Éditeur: Cell Press
DOI: 10.1016/j.stem.2022.04.011

Naive Pluripotent Stem Cells Exhibit Phenotypic Variability that Is Driven by Genetic Variation.

Auteurs: Ortmann D, Brown S, Czechanski A, Aydin S, Muraro D, Huang Y, Tomaz RA, Osnato A, Canu G, Wesley BT, Skelly DA, Stegle O, Choi T, Churchill GA, Baker CL, Rugg-Gunn PJ, Munger SC, Reinholdt LG, Vallier L
Publié dans: Cell Stem Cell, 2020, ISSN 1934-5909
Éditeur: Cell Press
DOI: 10.1016/j.stem.2020.07.019

Single-Cell Sequencing of Developing Human Gut Reveals Transcriptional Links to Childhood Crohn's Disease

Auteurs: Elmentaite R, Ross ADB, Roberts K, James KR, Ortmann D, Gomes T, Nayak K, Tuck L, Pritchard S, Bayraktar OA, Heuschkel R, Vallier L, Teichmann SA, Zilbauer M
Publié dans: Developmental Cell, 2021, ISSN 1534-5807
Éditeur: Cell Press
DOI: 10.1016/j.devcel.2020.11.010

TGFβ signalling is required to maintain pluripotency of human naïve pluripotent stem cells.

Auteurs: Osnato A, Brown S, Krueger C, Andrews S, Collier AJ, Nakanoh S, Quiroga Londoño M, Wesley BT, Muraro D, Brumm AS, Niakan KK, Vallier L*, Ortmann D*, Rugg-Gunn PJ*
Publié dans: Elife, 2021, ISSN 2050-084X
Éditeur: eLife Sciences Publications
DOI: 10.7554/elife.67259

Generation of functional hepatocytes by forward programming with nuclear receptors

Auteurs: Tomaz RA, Zacharis ED, Bachinger F, Wurmser A, Yamamoto D, Petrus-Reurer S, Morell CM, Dziedzicka D, Wesley BT, Geti I, Segeritz CP, de Brito MC, Chhatriwala M, Ortmann D, Saeb-Parsy K, Vallier L.
Publié dans: elife, 2022, ISSN 2050-084X
Éditeur: eLife Sciences Publications
DOI: 10.7554/elife.71591

Non-peer reviewed articles (1)

Modeling Disease with Human Inducible Pluripotent Stem Cells

Auteurs: Rodrigo Grandy, Rute A. Tomaz, Ludovic Vallier
Publié dans: Annual Review of Pathology: Mechanisms of Disease, Numéro 14/1, 2019, Page(s) 449-468, ISSN 1553-4006
Éditeur: Annual Reviews, Inc.
DOI: 10.1146/annurev-pathol-020117-043634

Book chapters (1)

Use of Biliary Organoids in Cholestasis Research

Auteurs: Fotios Sampaziotis, Olivia Tysoe, Teresa Brevini, Ludovic Vallier
Publié dans: Experimental Cholestasis Research, Numéro 1981, 2019, Page(s) 373-382, ISBN 978-1-4939-9419-9
Éditeur: Springer New York
DOI: 10.1007/978-1-4939-9420-5_25

Droits de propriété intellectuelle

Patent (3)

TRANSPLANTATION OF LIVER CELLS BY ADMINISTRATION TO THE BILIARY TREE OF THE LIVER

Numéro de demande/publication: 22 707076
Date: 2022-02-17
Demandeur(s): THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

IN VITRO PRODUCTION OF CHOLANGIOCYTES

Numéro de demande/publication: 18 733834
Date: 2016-06-21
Demandeur(s): THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

METHODS OF EXPANDING CHOLANGIOCYTES

Numéro de demande/publication: 18 733834
Date: 2018-06-19
Demandeur(s): THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE