Development of New therapies against cholangiopathies.

Informazioni relative al progetto

New Chol

ID dell’accordo di sovvenzione: 741707

DOI

10.3030/741707

Progetto chiuso

Data della firma CE 29 Novembre 2017

Data di avvio 1 Dicembre 2017

Data di completamento 30 Novembre 2023

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 2 499 111,00

Contributo UE

€ 2 499 111,00

Coordinato da

CHARITE - UNIVERSITAETSMEDIZIN BERLIN
Germany

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (35)

FXR inhibition may protect from SARS-CoV-2 infection by reducing ACE2

Autori: 109. Brevini T, Maes M, Webb GJ, John BV, Fuchs CD, Buescher G, Wang L, Griffiths C, Brown ML, Scott WE 3rd, Pereyra-Gerber P, Gelson WTH, Brown S, Dillon S, Muraro D, Sharp J, Neary M, Box H, Tatham L, Stewart J, Curley P, Pertinez H, Forrest S, Mlcochova P, Varankar SS, Darvish-Damavandi M, Mulcahy VL, Kuc RE, Williams TL, Heslop JA, Rossetti D, Tysoe OC, Galanakis V, Vila-Gonzalez M, Crozier TW
Pubblicato in: Nature, 2022, ISSN 1476-4687
Editore: springer
DOI: 10.1038/s41586-022-05594-0

Modeling HNF1B-associated monogenic diabetes using human iPSCs reveals an early stage impairment of the pancreatic developmental program.

Autori: El-Khairi R, Olszanowski E, Muraro D, Madrigal P, Tilgner K, Chhatriwala M, Vyas S, Chia CY, Vallier L*, Rodríguez-Seguí SA
Pubblicato in: Stem Cell Reports, 2021, ISSN 2213-6711
Editore: Cell Press
DOI: 10.1016/j.stemcr.2021.07.018

Single-cell atlas of human liver development reveals pathways directing hepatic cell fates.

Autori: Wesley BT, Ross ADB, Muraro D, Miao Z, Saxton S, Tomaz RA, Morell CM, Ridley K, Zacharis ED, Petrus-Reurer S, Kraiczy J, Mahbubani KT, Brown S, Garcia-Bernardo J, Alsinet C, Gaffney D, Horsfall D, Tysoe OC, Botting RA, Stephenson E, Popescu DM, MacParland S, Bader G, McGilvray ID, Ortmann D, Sampaziotis F, Saeb-Parsy K, Haniffa M, Stevens KR, Zilbauer M, Teichmann SA, Vallier L.
Pubblicato in: Nature Cell Biology, 2022, ISSN 1465-7392
Editore: Nature Publishing Group
DOI: 10.1038/s41556-022-00989-7

Unreveling the developmental roadmap toward human brown adipose tissue.

Autori: Stefania Carobbio 1, Anne-Claire Guenantin 2, Myriam Bahri 3, Sonia Rodriguez-Fdez 3, Floris Honig 3, Ioannis Kamzolas 4, Isabella Samuelson 2, Kathleen Long 3, Sherine Awad 5, Dunja Lukovic 6, Slaven Erceg 7, Andrew Bassett 3, Sasha Mendjan 8, Ludovic Vallier 9, Barry S Rosen 3, Davide Chiarugi 5, Antonio Vidal-Puig 10
Pubblicato in: Stem Cell Reports, 2021, ISSN 2213-6711
Editore: Cell Press
DOI: 10.1016/j.stemcr.2021.01.013

Cholangiocyte organoids can repair bile ducts after transplantation in the human liver

Autori: Sampaziotis F, Muraro D, Tysoe OC, Sawiak S, Beach TE, Godfrey EM, Upponi SS, Brevini T, Wesley BT, Garcia-Bernardo J, Mahbubani K, Canu G, Gieseck R 3rd, Berntsen NL, Mulcahy VL, Crick K, Fear C, Robinson S, Swift L, Gambardella L, Bargehr J, Ortmann D, Brown SE, Osnato A, Murphy MP, Corbett G, Gelson WTH, Mells GF, Humphreys P, Davies SE, Amin I, Gibbs P, Sinha S, Teichmann SA, Butler AJ, See TC
Pubblicato in: Science, 2021, ISSN 0036-8075
Editore: American Association for the Advancement of Science
DOI: 10.1126/science.aaz6964

Modeling Nonalcoholic Fatty Liver Disease in the Dish Using Human-Specific Platforms: Strategies and Limitations

Autori: Rezvani M, Vallier L, Guillot A
Pubblicato in: Cell Mol Gastroenterol Hepatol, 2023, ISSN 2352-345X
Editore: Elsevier Inc.
DOI: 10.1016/j.jcmgh.2023.01.014

Modeling: In Vitro Pancreatic Differentiation From Human Pluripotent Stem Cells Gains Momentum

Autori: Burgos JI, Vallier L, Rodríguez-Seguí SA.
Pubblicato in: Frontiers in Endocrinology, 2021, ISSN 1664-2392
Editore: Frontiers
DOI: 10.3389/fendo.2021.692596

Single-cell transcriptomic characterization of a gastrulating human embryo

Autori: Tyser RCV, Mahammadov E, Nakanoh S, Vallier L, Scialdone A, Srinivas S
Pubblicato in: Nature, 2021, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41586-021-04158-y

Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications.

Autori: Vitillo L, Vallier L
Pubblicato in: Methods Mol Biol, 2021, ISSN 1064-3745
Editore: Humana Press, Inc.
DOI: 10.1007/7651_2021_401

Organoids capture tissue-specific innate lymphoid cell development in mice and humans.

Autori: Jowett GM, Read E, Roberts LB, Coman D, Vilà González M, Zabinski T, Niazi U, Reis R, Trieu TJ, Danovi D, Gentleman E, Vallier L, Curtis MA, Lord GM, Neves JF.
Pubblicato in: Cell Reports, 2022, ISSN 2211-1247
Editore: Cell Press
DOI: 10.1016/j.celrep.2022.111281

PNPLA3-Associated NAFLD Using Human-Induced Pluripotent Stem Cells.

Autori: Tilson SG, Morell CM, Lenaerts AS, Park SB, Hu Z, Jenkins B, Koulman A, Liang TJ*, Vallier L
Pubblicato in: Heptology, 2021, ISSN 2471-254X
Editore: American Association for the Study of Liver Diseases
DOI: 10.1002/hep.32063

GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells.

Autori: 87. Vitillo L, Durance C, Hewitt Z, Moore H, Smith A, Vallier L.
Pubblicato in: Stem Cell Research Therapy, 2020, ISSN 1757-6512
Editore: BioMed Central
DOI: 10.1186/s13287-020-01915-0

Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids.

Autori: Marsee A, Roos FJM, Verstegen MMA; HPB Organoid Consortium, Gehart H, de Koning E, Lemaigre F, Forbes SJ, Peng WC, Huch M, Takebe T, Vallier L, Clevers H, van der Laan LJW, Spee B
Pubblicato in: Cell Stem Cell, 2021, ISSN 1934-5909
Editore: Cell Press
DOI: 10.1016/j.stem.2021.04.005

hiPSC hepatocyte model demonstrates the role of unfolded protein response and inflammatory networks in α1-antitrypsin deficiency

Autori: Charis-Patricia Segeritz, Sheikh Tamir Rashid, Miguel Cardoso de Brito, Maria Paola Serra, Adriana Ordonez, Carola Maria Morell, Joseph E. Kaserman, Pedro Madrigal, Nicholas R.F. Hannan, Laurent Gatto, Lu Tan, Andrew A. Wilson, Kathryn Lilley, Stefan J. Marciniak, Bibek Gooptu, David A. Lomas, Ludovic Vallier
Pubblicato in: Journal of Hepatology, Numero 69/4, 2018, Pagina/e 851-860, ISSN 0168-8278
Editore: Elsevier BV
DOI: 10.1016/j.jhep.2018.05.028

HNF4A Haploinsufficiency in MODY1 Abrogates Liver and Pancreas Differentiation from Patient-Derived Induced Pluripotent Stem Cells

Autori: Natasha Hui Jin Ng, Joanita Binte Jasmen, Chang Siang Lim, Hwee Hui Lau, Vidhya Gomathi Krishnan, Juned Kadiwala, Rohit N. Kulkarni, Helge Ræder, Ludovic Vallier, Shawn Hoon, Adrian Kee Keong Teo
Pubblicato in: iScience, Numero 16, 2019, Pagina/e 192-205, ISSN 2589-0042
Editore: Elsevier
DOI: 10.1016/j.isci.2019.05.032

Combined single-cell profiling of expression and DNA methylation reveals splicing regulation and heterogeneity

Autori: Stephanie M. Linker, Lara Urban, Stephen J. Clark, Mariya Chhatriwala, Shradha Amatya, Davis J. McCarthy, Ingo Ebersberger, Ludovic Vallier, Wolf Reik, Oliver Stegle, Marc Jan Bonder
Pubblicato in: Genome Biology, Numero 20/1, 2019, ISSN 1474-760X
Editore: BMC
DOI: 10.1186/s13059-019-1644-0

Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue

Autori: Olivia C. Tysoe, Alexander W. Justin, Teresa Brevini, Si Emma Chen, Krishnaa T. Mahbubani, Anna K. Frank, Hajer Zedira, Espen Melum, Kourosh Saeb-Parsy, Athina E. Markaki, Ludovic Vallier, Fotios Sampaziotis
Pubblicato in: Nature Protocols, Numero 14/6, 2019, Pagina/e 1884-1925, ISSN 1754-2189
Editore: Nature Publishing Group
DOI: 10.1038/s41596-019-0168-0

Genome-Wide Epigenetic and Transcriptomic Characterization of Human-Induced Pluripotent Stem Cell–Derived Intestinal Epithelial Organoids

Autori: Judith Kraiczy, Alexander D.B. Ross, Jessica L. Forbester, Gordon Dougan, Ludovic Vallier, Matthias Zilbauer
Pubblicato in: Cellular and Molecular Gastroenterology and Hepatology, Numero 7/2, 2019, Pagina/e 285-288, ISSN 2352-345X
Editore: Elsevier Inc.
DOI: 10.1016/j.jcmgh.2018.10.008

Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics

Autori: Loukia Yiangou, Rodrigo A. Grandy, Carola M. Morell, Rute A. Tomaz, Anna Osnato, Juned Kadiwala, Daniele Muraro, Jose Garcia-Bernardo, Shota Nakanoh, William G. Bernard, Daniel Ortmann, Davis J. McCarthy, Ingrid Simonic, Sanjay Sinha, Ludovic Vallier
Pubblicato in: Stem Cell Reports, Numero 12/1, 2019, Pagina/e 165-179, ISSN 2213-6711
Editore: Cell Press
DOI: 10.1016/j.stemcr.2018.11.020

Human Pluripotent Stem Cell-Derived Endoderm for Modeling Development and Clinical Applications

Autori: Loukia Yiangou, Alexander D.B. Ross, Kim Jee Goh, Ludovic Vallier
Pubblicato in: Cell Stem Cell, Numero 22/4, 2018, Pagina/e 485-499, ISSN 1934-5909
Editore: Cell Press
DOI: 10.1016/j.stem.2018.03.016

Regenerative cell therapy for the treatment of hyperbilirubinemic Gunn rats with fresh and frozen human induced pluripotent stem cells‐derived hepatic stem cells

Autori: Angélique Fourrier, Frédéric Delbos, Séverine Menoret, Camille Collet, Linh Trinh Thi Thuy, Anne Myara, François Petit, Laia Tolosa, Sophie Laplanche, María José Gómez‐Lechón, Philippe Labrune, Ignacio Anegon, Ludovic Vallier, Delphine Garnier, Tuan Huy Nguyen
Pubblicato in: Xenotransplantation, 2019, ISSN 0908-665X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/xen.12544

Advances in the generation of bioengineered bile ducts

Autori: Alexander W. Justin, Kourosh Saeb-Parsy, Athina E. Markaki, Ludovic Vallier, Fotios Sampaziotis
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Numero 1864/4, 2018, Pagina/e 1532-1538, ISSN 0925-4439
Editore: Elsevier BV
DOI: 10.1016/j.bbadis.2017.10.034

The SMAD2/3 interactome reveals that TGFβ controls m6A mRNA methylation in pluripotency

Autori: Alessandro Bertero, Stephanie Brown, Pedro Madrigal, Anna Osnato, Daniel Ortmann, Loukia Yiangou, Juned Kadiwala, Nina C. Hubner, Igor Ruiz de los Mozos, Christoph Sadée, An-Sofie Lenaerts, Shota Nakanoh, Rodrigo Grandy, Edward Farnell, Jernej Ule, Hendrik G. Stunnenberg, Sasha Mendjan, Ludovic Vallier
Pubblicato in: Nature, Numero 555/7695, 2018, Pagina/e 256-259, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/nature25784

Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression

Autori: Anna S. E. Cuomo, Daniel D. Seaton, Davis J. McCarthy, Iker Martinez, Marc Jan Bonder, Jose Garcia-Bernardo, Shradha Amatya, Pedro Madrigal, Abigail Isaacson, Florian Buettner, Andrew Knights, Kedar Nath Natarajan, Ludovic Vallier, John C. Marioni, Mariya Chhatriwala, Oliver Stegle
Pubblicato in: Nature Communications, Numero 11/1, 2020, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-020-14457-z

A Novel Chemically Differentiated Mouse Embryonic Stem Cell-Based Model to Study Liver Stages of Plasmodium berghei

Autori: Jaishree Tripathi, Charis-Patricia Segeritz, Gareth Griffiths, Wendy Bushell, Ludovic Vallier, William C. Skarnes, Maria M. Mota, Oliver Billker
Pubblicato in: Stem Cell Reports, Numero 14/6, 2020, Pagina/e 1123-1134, ISSN 2213-6711
Editore: Cell Press
DOI: 10.1016/j.stemcr.2020.04.010

GDF15 mediates the effects of metformin on body weight and energy balance

Autori: Anthony P. Coll, Michael Chen, Pranali Taskar, Debra Rimmington, Satish Patel, John A. Tadross, Irene Cimino, Ming Yang, Paul Welsh, Samuel Virtue, Deborah A. Goldspink, Emily L. Miedzybrodzka, Adam R. Konopka, Raul Ruiz Esponda, Jeffrey T.-J. Huang, Y. C. Loraine Tung, Sergio Rodriguez-Cuenca, Rute A. Tomaz, Heather P. Harding, Audrey Melvin, Giles S. H. Yeo, David Preiss, Antonio Vidal-Puig, Lud
Pubblicato in: Nature, Numero 578/7795, 2020, Pagina/e 444-448, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41586-019-1911-y

SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes

Autori: Waradon Sungnak, Ni Huang, Christophe Bécavin, Marijn Berg, Rachel Queen, Monika Litvinukova, Carlos Talavera-López, Henrike Maatz, Daniel Reichart, Fotios Sampaziotis, Kaylee B. Worlock, Masahiro Yoshida, Josephine L. Barnes
Pubblicato in: Nature Medicine, Numero 26/5, 2020, Pagina/e 681-687, ISSN 1078-8956
Editore: Nature Publishing Group
DOI: 10.1038/s41591-020-0868-6

Regional differences in human biliary tissues and corresponding in vitro derived organoids

Autori: Casey A Rimland, Samantha G Tilson, Carola M Morell, Rute A Tomaz, Wei-Yu Lu, Simone E Adams, Nikitas Georgakopoulos, Francisco Otaizo-Carrasquero, Timothy G Myers, John R Ferdinand, Richard L Gieseck, Fotios Sampaziotis, Olivia C Tysoe, Brandon Wesley, Daniele Muraro, Gabriel C Oniscu, Nicholas RF Hannan, Stuart J Forbes, Kourosh Saeb-Parsy, Thomas A Wynn, Ludovic Vallier
Pubblicato in: Hepatology, 2020, ISSN 0270-9139
Editore: John Wiley & Sons Inc.
DOI: 10.1002/hep.31252

Analysis of endothelial-to-haematopoietic transition at the single cell level identifies cell cycle regulation as a driver of differentiation

Autori: Giovanni Canu, Emmanouil Athanasiadis, Rodrigo A. Grandy, Jose Garcia-Bernardo, Paulina M. Strzelecka, Ludovic Vallier, Daniel Ortmann, Ana Cvejic
Pubblicato in: Genome Biology, Numero 21/1, 2020, ISSN 1474-760X
Editore: Springer Nature
DOI: 10.1186/s13059-020-02058-4

Cell cycle regulators control mesoderm specification in human pluripotent stem cells

Autori: Loukia Yiangou, Rodrigo A. Grandy, Anna Osnato, Daniel Ortmann, Sanjay Sinha, Ludovic Vallier
Pubblicato in: Journal of Biological Chemistry, Numero 294/47, 2019, Pagina/e 17903-17914, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra119.008251

Human branching cholangiocyte organoids recapitulate functional bile duct formation.

Autori: 107. Roos FJM, van Tienderen GS, Wu H, Bordeu I, Vinke D, Albarinos LM, Monfils K, Niesten S, Smits R, Willemse J, Rosmark O, Westergren-Thorsson G, Kunz DJ, de Wit M, French PJ, Vallier L, IJzermans JNM, Bartfai R, Marks H, Simons BD, van Royen ME, Verstegen MMA, van der Laan LJW
Pubblicato in: Cell Stem Cell, 2022, ISSN 1934-5909
Editore: Cell Press
DOI: 10.1016/j.stem.2022.04.011

Naive Pluripotent Stem Cells Exhibit Phenotypic Variability that Is Driven by Genetic Variation.

Autori: Ortmann D, Brown S, Czechanski A, Aydin S, Muraro D, Huang Y, Tomaz RA, Osnato A, Canu G, Wesley BT, Skelly DA, Stegle O, Choi T, Churchill GA, Baker CL, Rugg-Gunn PJ, Munger SC, Reinholdt LG, Vallier L
Pubblicato in: Cell Stem Cell, 2020, ISSN 1934-5909
Editore: Cell Press
DOI: 10.1016/j.stem.2020.07.019

Single-Cell Sequencing of Developing Human Gut Reveals Transcriptional Links to Childhood Crohn's Disease

Autori: Elmentaite R, Ross ADB, Roberts K, James KR, Ortmann D, Gomes T, Nayak K, Tuck L, Pritchard S, Bayraktar OA, Heuschkel R, Vallier L, Teichmann SA, Zilbauer M
Pubblicato in: Developmental Cell, 2021, ISSN 1534-5807
Editore: Cell Press
DOI: 10.1016/j.devcel.2020.11.010

TGFβ signalling is required to maintain pluripotency of human naïve pluripotent stem cells.

Autori: Osnato A, Brown S, Krueger C, Andrews S, Collier AJ, Nakanoh S, Quiroga Londoño M, Wesley BT, Muraro D, Brumm AS, Niakan KK, Vallier L*, Ortmann D*, Rugg-Gunn PJ*
Pubblicato in: Elife, 2021, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.67259

Generation of functional hepatocytes by forward programming with nuclear receptors

Autori: Tomaz RA, Zacharis ED, Bachinger F, Wurmser A, Yamamoto D, Petrus-Reurer S, Morell CM, Dziedzicka D, Wesley BT, Geti I, Segeritz CP, de Brito MC, Chhatriwala M, Ortmann D, Saeb-Parsy K, Vallier L.
Pubblicato in: elife, 2022, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.71591

Non-peer reviewed articles (1)

Modeling Disease with Human Inducible Pluripotent Stem Cells

Autori: Rodrigo Grandy, Rute A. Tomaz, Ludovic Vallier
Pubblicato in: Annual Review of Pathology: Mechanisms of Disease, Numero 14/1, 2019, Pagina/e 449-468, ISSN 1553-4006
Editore: Annual Reviews, Inc.
DOI: 10.1146/annurev-pathol-020117-043634

Book chapters (1)

Use of Biliary Organoids in Cholestasis Research

Autori: Fotios Sampaziotis, Olivia Tysoe, Teresa Brevini, Ludovic Vallier
Pubblicato in: Experimental Cholestasis Research, Numero 1981, 2019, Pagina/e 373-382, ISBN 978-1-4939-9419-9
Editore: Springer New York
DOI: 10.1007/978-1-4939-9420-5_25

Diritti di proprietà intellettuale

Patent (3)

TRANSPLANTATION OF LIVER CELLS BY ADMINISTRATION TO THE BILIARY TREE OF THE LIVER

Numero candidatura/pubblicazione: 22 707076
Data: 2022-02-17
Candidato/i: THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

IN VITRO PRODUCTION OF CHOLANGIOCYTES

Numero candidatura/pubblicazione: 18 733834
Data: 2016-06-21
Candidato/i: THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

METHODS OF EXPANDING CHOLANGIOCYTES

Numero candidatura/pubblicazione: 18 733834
Data: 2018-06-19
Candidato/i: THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE