Tailored chemical complexity through evolution-inspired synthetic biology

Project Information

SynPlex

Grant agreement ID: 742739

DOI 10.3030/742739

Project closed

EC signature date 19 May 2017

Start date 1 August 2017

End date 31 July 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 495 755,00

EU contribution € 2 495 755,00

Coordinated by

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland

Publications

Peer reviewed articles (21)

A multiproducer microbiome generates chemical diversity in the marine sponge Mycale hentscheli

Author(s): Michael Rust; Eric J. N. Helfrich; Michael F. Freeman; Pakjira Nanudorn; Christopher M. Field; Christian Rückert; Tomas Kündig; Michael J. Page; Victoria L. Webb; Jörn Kalinowski; Shinichi Sunagawa; Jörn Piel
Published in: Proceedings of the National Academy of Sciences of the United States of America, Issue 2, 2020, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.3929/ethz-b-000413997

Modular Halogenation, α-Hydroxylation, and Acylation by a Remarkably Versatile Polyketide Synthase

Author(s): Dr. Franziska Hemmerling,Dr. Roy A. Meoded,Dr. Amy E. Fraley,Hannah A. Minas,Cora L. Dieterich,Dr. Michael Rust,Dr. Reiko Ueoka,Dr. Katja Jensen,Prof. Dr. Eric J. N. Helfrich,Cedric Bergande,Maurice Biedermann,Dr. Nancy Magnus,Prof. Dr. Birgit Piechulla, Prof. Jörn Piel
Published in: Angewandte Chemie International Edition, 2022, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202116614

Comparative Genomics Underlines Multiple Roles of Profftella, an Obligate Symbiont of Psyllids: Providing Toxins, Vitamins, and Carotenoids.

Author(s): Atsushi Nakabachi; Jörn Piel; Igor Malenovský; Yuu Hirose
Published in: Genome Biology and Evolution, Issue 17596653, 2020, Page(s) 1975–1987, ISSN 1759-6653
Publisher: Oxford University Press
DOI: 10.3929/ethz-b-000451415

In Vivo Production of Diverse beta-Amino Acid-Containing Proteins

Author(s): Edgars Lakis; Sarolt Magyari; Jörn Piel
Published in: Angewandte Chemie. International Edition, 61 (29), Issue 14337851, 2022, Page(s) e202202695, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202202695

Toblerols, Cyclopropanol-Containing Modulators of Methylobacterial Antibiosis Generated by an Unusual Polyketide Synthase

Author(s): Ueoka, Reiko Bortfeld-Miller, Miriam Morinaka, Brandon I. Vorholt, Julia A. Piel, Jörn
Published in: Angewandte Chemie (international Edition), 2017, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201709056

Genome-based discovery and total synthesis of janustatins, potent cytotoxins from a plant-associated bacterium

Author(s): Reiko Ueoka, Philipp Sondermann, Stefan Leopold-Messer, Yizhou Liu, Rei Suo, Agneya Bhushan, Lida Vadakumchery, Ute Greczmiel, Yoko Yashiroda, Hiromi Kimura, Shinichi Nishimura, Yojiro Hoshikawa, Minoru Yoshida, Annette Oxenius, Shigeki Matsunaga, R. Thomas Williamson, Erick M. Carreira, Jörn Piel
Published in: Nature Chemistry, Issue 17554330, 2022, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/s41557-022-01020-0

Engineering the stambomycin modular polyketide synthase yields 37-membered mini-stambomycins

Author(s): Li Su, Laurence Hôtel, Cédric Paris, Clara Chepkirui, Alexander O Brachmann, Jörn Piel, Christophe Jacob, Bertrand Aigle, Kira J Weissman
Published in: Nature Communications, Issue 20411723, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-27955-z

Opening up the Single‐Cell Toolbox for Microbial Natural Products Research

Author(s): Jackson K. B. Cahn; Jörn Piel
Published in: Angewandte Chemie International Edition, Issue 14337851, 2021, Page(s) 18412-18428, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201900532

Genome Mining of Oxidation Modules in trans ‐Acyltransferase Polyketide Synthases Reveals a Culturable Source for Lobatamides

Author(s): Reiko Ueoka, Roy A. Meoded, Alejandro Gran‐Scheuch, Agneya Bhushan, Marco W. Fraaije, Jörn Piel
Published in: Angewandte Chemie International Edition, Issue 59/20, 2020, Page(s) 7761-7765, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201916005

A Polyketide Synthase Component for Oxygen Insertion into Polyketide Backbones

Author(s): Roy A. Meoded, Reiko Ueoka, Eric J. N. Helfrich, Katja Jensen, Nancy Magnus, Birgit Piechulla, Jörn Piel
Published in: Angewandte Chemie International Edition, Issue 57/36, 2018, Page(s) 11644-11648, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201805363

Bipartite interactions, antibiotic production and biosynthetic potential of the Arabidopsis leaf microbiome

Author(s): Eric J. N. Helfrich, Christine M. Vogel, Reiko Ueoka, Martin Schäfer, Florian Ryffel, Daniel B. Müller, Silke Probst, Markus Kreuzer, Jörn Piel, Julia A. Vorholt
Published in: Nature Microbiology, Issue 3/8, 2018, Page(s) 909-919, ISSN 2058-5276
Publisher: Nature Research
DOI: 10.1038/s41564-018-0200-0

Natural noncanonical protein splicing yields products with diverse β-amino acid residues

Author(s): Brandon I. Morinaka, Edgars Lakis, Marjan Verest, Maximilian J. Helf, Thibault Scalvenzi, Anna L. Vagstad, James Sims, Shinichi Sunagawa, Muriel Gugger, Jörn Piel
Published in: Science, Issue 359/6377, 2018, Page(s) 779-782, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.aao0157

Genome-Based Identification of a Plant-Associated Marine Bacterium as a Rich Natural Product Source

Author(s): Reiko Ueoka, Agneya Bhushan, Silke I. Probst, Walter M. Bray, R. Scott Lokey, Roger G. Linington, Jörn Piel
Published in: Angewandte Chemie International Edition, Issue 57/44, 2018, Page(s) 14519-14523, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201805673

Structural and Functional Studies of a Pyran Synthase Domain from a trans -Acyltransferase Assembly Line

Author(s): Drew T. Wagner, Zhicheng Zhang, Roy A. Meoded, Alexis J. Cepeda, Jörn Piel, Adrian T. Keatinge-Clay
Published in: ACS Chemical Biology, Issue 13/4, 2018, Page(s) 975-983, ISSN 1554-8929
Publisher: American Chemical Society
DOI: 10.1021/acschembio.8b00049

Automated structure prediction of trans-acyltransferase polyketide synthase products

Author(s): Eric J. N. Helfrich, Reiko Ueoka, Alon Dolev, Michael Rust, Roy A. Meoded, Agneya Bhushan, Gianmaria Califano, Rodrigo Costa, Muriel Gugger, Christoph Steinbeck, Pablo Moreno, Jörn Piel
Published in: Nature Chemical Biology, Issue 15/8, 2019, Page(s) 813-821, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-019-0313-7

Effect of tolytoxin on tunneling nanotube formation and function

Author(s): Aysegul Dilsizoglu Senol, Anna Pepe, Clara Grudina, Nathalie Sassoon, Ueoka Reiko, Luc Bousset, Ronald Melki, Jörn Piel, Muriel Gugger, Chiara Zurzolo
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-42161-6

Genome mining- and synthetic biology-enabled production of hypermodified peptides

Author(s): Agneya Bhushan, Peter J. Egli, Eike E. Peters, Michael F. Freeman, Jörn Piel
Published in: Nature Chemistry, Issue 11/10, 2019, Page(s) 931-939, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/s41557-019-0323-9

The hidden enzymology of bacterial natural product biosynthesis

Author(s): Thomas A. Scott, Jörn Piel
Published in: Nature Reviews Chemistry, Issue 3/7, 2019, Page(s) 404-425, ISSN 2397-3358
Publisher: Nature Research
DOI: 10.1038/s41570-019-0107-1

Structure of a Promiscuous Thioesterase Domain Responsible for Branching Acylation in Polyketide Biosynthesis

Author(s): Amy E. Fraley, Cora L. Dieterich, Mathijs F. J. Mabesoone, Hannah A. Minas, Roy A Meoded, Franziska Hemmerling, Jörn Piel
Published in: Angewandte Chemie International Edition, Issue 14337851, 2022, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202206385

Evolution of combinatorial diversity in trans-acyltransferase polyketide synthase assembly lines across bacteria

Author(s): Eric J. N. Helfrich, Reiko Ueoka, Marc G. Chevrette, Franziska Hemmerling, Xiaowen Lu, Stefan Leopold-Messer, Hannah A. Minas, Adrien Y. Burch, Steven E. Lindow, Jörn Piel & Marnix H. Medema
Published in: Nature Communications, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-21163-x

Strategies to access biosynthetic novelty in bacterial genomes for drug discovery

Author(s): Franziska Hemmerling; Jörn Piel
Published in: Nature Reviews Drug Discovery, 21 (5), Issue 14741776, 2022, ISSN 1474-1776
Publisher: Nature Publishing Group
DOI: 10.1038/s41573-022-00414-6

Intellectual Property Rights

Patent (3)

USE OF RADICAL S-ADENOSYL METHIONINE (SAM) ENZYMES FOR INTRODUCING α-KETO-β3-AMINO ACIDS INTO (POLY)PEPTIDES

Application/Publication number: 20 18050225
Date: 2018-01-05

USE OF RADICAL S-ADENOSYL METHIONINE (SAM) ENZYMES FOR INTRODUCING α-KETO-β3-AMINO ACIDS INTO (POLY)PEPTIDES

Application/Publication number: 20 18050225
Date: 2018-01-05

USE OF RADICAL S-ADENOSYL METHIONINE (SAM) ENZYMES FOR INTRODUCING α-KETO-β3-AMINO ACIDS INTO (POLY)PEPTIDES

Application/Publication number: 20 18050225
Date: 2018-01-05