The project ACTIVEMOTION3D is strongly based upon the proposed state-of-the-art light sheet microscope to perform experiments on microswimmers in 3D. The secondment at ICFO-The institute of Photonic Sciences played a crucial role in learning the necessary skills to build the customised light-sheet microscope at the host institute (University of Gothenburg-UGOT). The overall design and imaging parameters of the set-up, such as resolution, speed and magnification were optimised based on the objectives of the project.
Following the successful secondment at ICFO in the beginning of the project, the set-up was successfully implemented at UGOT. The basic light-sheet microscope was functioning by the end of the first year and only the wavefront-coding method remained to be implemented. The wavefront-coding method is crucial to the final aim of the project in terms of imaging speed required to monitor artificial microswimmers in complex environments. The implementation of the wavefront-coding method was specifically challenging and required more time.
The implemented light-sheet microscope was also used for establishing new collaborations, oriented towards interdisciplinary research. One of the most exciting development of modern micro-bio-technology are organoids, where organ specific cells are cultured in 3D cultures, thus representing more physiologically relevant cellular properties compared to conventional 2D cell cultures. These organoids offer several advantages in terms of studying organ specific cells and their response to toxic and non-toxic drugs among many others. However, current methods require physical sectioning of these organoids to study their properties and responses, meaning separating the cells from their physiologically relevant environments. This restricts the scope of such studies because the cells have to be taken out of the organoids for various physiologically relevant studies and several studies can not be conducted on the cells from the same organoids.
The newly established light-sheet microscope was used for proof-of-principle experiments on liver organoids (acquired from collaborators at the university hospital) to demonstrate the advantages of optical sectioning in acquiring live images of biological samples. It was shown that the possibility to eliminate physical sectioning of organoids open up new avenues for more detailed study of cells growing in 3D, such as organ development and disease progression in organoids with infected cells.
