Epigenetically Stable Cloned Plants: Increasing The Epigenetic and Phenotypic Diversity of Plants through Tissue-Specific Regeneration

Projektinformationen

Epi-Somaclone

ID Finanzhilfevereinbarung: 751204

DOI 10.3030/751204

Abgeschlossenes Projekt

EK-Unterschriftsdatum 27 Februar 2017

Startdatum 1 Oktober 2017

Enddatum 31 Dezember 2019

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten € 171 460,80

EU-Beitrag € 171 460,80

Koordiniert durch

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Germany

Ziel

Most of phenotypic variation comes from variation in DNA sequence. However, a number of studies have clearly shown that phenotype variation and diversity can also be the result of epigenetic changes. The major goal of this proposal is to develop a novel methodology for robust production of epigenetically stable plant variants, in order to increase epigenetic and phenotypic diversity. A key element in the project will be a recent breakthrough in the generation of clonal individuals from different tissue types using inducible overexpression of the embryonic regulatory protein RKD4. Preliminary work shows that plants regenerated from roots inherit many aspects of root specific methylation and gene expression patterns, even in the leaf tissue. Importantly, these features are stably transmitted across generations. For this project, I propose to: (1) evaluate physiological consequences of altered epigenomes in RKD4 regenerated plants; (2) compare (epi)genomic and phenotypic variation between plants regenerated using hormones and RKD4 overexpression; (3) compare epigenomic and phenotypic consequences in regenerants of different Arabidopsis thaliana accessions and close A. thaliana relatives Arabidopsis lyrata and Capsella rubella. The program, which will be carried out under supervision of Detlef Weigel, a world leader in plant genetics and genomics, will substantially contribute to increasing the toolkit for plant breeders to generate meaningful phenotypic diversity.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › genetics › DNA
• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › biological sciences › genetics › mutation
• natural sciences › biological sciences › genetics › genomes
• natural sciences › biological sciences › genetics › epigenetics

Programm/Programme

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thema/Themen

• MSCA-IF-2016 - Individual Fellowships

Aufforderung zur Vorschlagseinreichung

H2020-MSCA-IF-2016

Finanzierungsplan

MSCA-IF-EF-ST - Standard EF

Koordinator

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV

• Netto-EU-Beitrag: € 171 460,80
• Adresse: HOFGARTENSTRASSE 8 ; 80539 Munchen ; Deutschland
• Region: Bayern › Oberbayern › München, Kreisfreie Stadt
• Aktivitätstyp: Research Organisations
• Gesamtkosten: € 171 460,80