Periodic Reporting for period 1 - SPLICANCER (Regulation and reprogramming of alternative splicing in cellular transformation)
Periodo di rendicontazione: 2017-07-01 al 2019-06-30
The aim of this project was to study the physiological mechanism behind alternative splicing of myosin VI in order to understand its de-regulation in tumors and cancer progression.
The first part of the project was focused on the identification of proteins that can bind the myosin VI pre-mRNA and, consequently, regulate the AS of myosin VI in physiological conditions. We employed different approaches, and identified proteins that regulate the AS of myosin VI.
The second part of the project, instead, was focused on the identification of signaling pathways that influence the AS of myosin VI in physiological conditions. We identified such pathway, and we are currently analyzing additional splicing-regulated targets and their possible implications in the de-regulation of AS in cancer.
The results achieved with this project were presented to the scientific community, the educational community and the civil society. We share scientific information with the society by participating in the PubScience initiative organized in Milan, Italy.
No website has been developed for the project.
This project will also impact the state-of-the art of the techniques applied by scientist working in the RNA field, thanks to the development of a new in vivo technology for the identification of proteins that bind mRNAs in a physiological context.