Protein nano-patterning using DNA nanotechnology; control of surface-based immune system activation

Project Information

BioNanoPattern

Grant agreement ID: 759517

DOI

10.3030/759517

Project closed

EC signature date 12 September 2017

Start date 1 January 2018

End date 31 December 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 499 850,00

EU contribution

€ 1 499 850,00

Coordinated by

ACADEMISCH ZIEKENHUIS LEIDEN
Netherlands

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (18)

Selecting optimal support grids for super-resolution cryogenic correlated light and electron microscopy

Author(s): Mart G. F. Last, Maarten W. Tuijtel, Lenard M. Voortman, Thomas H. Sharp
Published in: Scientific Reports, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-35590-x

Alternative Polyadenylation Utilization Results in Ribosome Assembly and mRNA Translation Deficiencies in a Model for Muscle Aging

Author(s): Hailiang Mei, PhD, Jasper Boom, BSc, Salma el Abdellaoui, BSc, Kotb Abdelmohsen, PhD, Rachel Munk, MSc, Jennifer L Martindale, MSc, Susan Kloet, PhD, Szymone M Kielbasa, PhD, Thomas H Sharp, PhD, Myriam Gorospe, PhD, Vered Raz, PhD
Published in: The Journals of Gerontology: Series A, 2022, ISSN 1079-5006
Publisher: Gerontological Society of America
DOI: 10.1093/gerona/glac058

Cryo-Electron Microscopy and Biochemical Analysis Offer Insights Into the Effects of Acidic pH, Such as Occur During Acidosis, on the Complement Binding Properties of C-Reactive Protein

Author(s): Dylan P. Noone, Tijn T. van der Velden and Thomas H. Sharp
Published in: Frontiers in Immunology, 2021, ISSN 1664-3224
Publisher: Frontiers
DOI: 10.3389/fimmu.2021.757633

Human anti-C1q autoantibodies bind specifically to solid-phase C1q and enhance phagocytosis but not complement activation

Author(s): Douwe J Dijkstra, Fleur S van de Bovenkamp, Leoni Abendstein, Rob Zuijderduijn, Jos Pool, Cynthia SM Kramer, Linda M Slot, Jan W Drijfhout, Lisanne de Vor, Kyra A Gelderman, Suzan HM Rooijakkers, Arnaud Zaldumbide, Gestur Vidarsson, Thomas H Sharp, Paul WHI Parren, Leendert A Trouw
Published in: PNAS, 2024, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2310666120

PTX3 structure determination using a hybrid cryoelectron microscopy and AlphaFold approach offers insights into ligand binding and complement activation

Author(s): Dylan P. Noone, Douwe J. Dijkstra, Teun T. van der Klugt https, Peter A. van Veelen, Arnoud H. de Ru, Paul J. Hensbergen, Leendert A. Trouw, and Thomas H. Sharp
Published in: Proceedings of the National Academy of Sciences, USA, 2022, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.220814411

Super-resolution fluorescence imaging of cryosamples does not limit achievable resolution in cryoEM

Author(s): Mart G.F. Last; Willem E.M. Noteborn; Lenard M. Voortman; Thomas H. Sharp
Published in: Journal of Structural Biology, Issue 2, 2023, ISSN 1047-8477
Publisher: Academic Press
DOI: 10.1016/j.jsb.2023.108040

Efficient mRNA delivery using lipid nanoparticles modified with fusogenic coiled-coil peptides

Author(s): Ye Zeng, Mengjie Shen, Roy Pattipeiluhu, Xuequan Zhou, Yun Zhang, Thomas Bakkum, Thomas H Sharp, Aimee L Boyle, Alexander Kros
Published in: Nanoscale, 2023, ISSN 2040-3372
Publisher: Royal Society of Chemistry
DOI: 10.1039/d3nr02175k

Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins

Author(s): Maarten W. Tuijtel, Abraham J. Koster, Stefan Jakobs, Frank G. A. Faas, Thomas H. Sharp
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-37728-8

Carbamylation reduces the capacity of IgG for hexamerization and complement activation

Author(s): R. Lubbers, S. C. Oostindie, D. J. Dijkstra, P. W. H. I. Parren, M. K. Verheul, L. Abendstein, T. H. Sharp, A. Ru, G. M. C. Janssen, P. A. Veelen, E. T. J. den Bremer, B. Bleijlevens, B.‐J. Kreuk, F. J. Beurskens, L. A. Trouw
Published in: Clinical & Experimental Immunology, Issue 200/1, 2020, Page(s) 1-11, ISSN 0009-9104
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/cei.13411

scNodes: a correlation and processing toolkit for super-resolution fluorescence and electron microscopy

Author(s): Mart G. F. Last; Lenard M. Voortman; Thomas H. Sharp
Published in: Nature Methods, 2023, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-023-01991-z

Insights into IgM-mediated complement activation based on in situ structures of IgM-C1-C4b

Author(s): Thomas H. Sharp, Aimee L. Boyle, Christoph A. Diebolder, Alexander Kros, Abraham J. Koster, Piet Gros
Published in: Proceedings of the National Academy of Sciences, 2019, Page(s) 201901841, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1901841116

Liquid crystalline inverted lipid phases encapsulating siRNA enhance lipid nanoparticle mediated transfection

Author(s): Roy Pattipeiluhu, Ye Zeng, Marco M.R.M. Hendrix, Ilja K. Voets, Alexander Kros, Thomas H. Sharp
Published in: Nature Communications, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-45666-5

One-Pot Synthesis of Defined-Length ssDNA for Multiscaffold DNA Origami

Author(s): Willem E. M. Noteborn, Leoni Abendstein, Thomas H. Sharp
Published in: Bioconjugate Chemistry, Issue 32/1, 2021, Page(s) 94-98, ISSN 1043-1802
Publisher: American Chemical Society
DOI: 10.1021/acs.bioconjchem.0c00644

Anionic Lipid Nanoparticles Preferentially Deliver mRNA to the Hepatic Reticuloendothelial System

Author(s): Roy Pattipeiluhu,Gabriela Arias-Alpizar,Genc Basha,Karen Y. T. Chan,Jeroen Bussmann,Thomas H. Sharp,Mohammad-Amin Moradi,Nico Sommerdijk,Edward N. Harris,Pieter R. Cullis,Alexander Kros,Dominik Witzigmann,Frederick Campbell
Published in: Advanced Materials, 2022, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202201095

Measuring cryo-TEM sample thickness using reflected light microscopy and machine learning

Author(s): Mart G F, Last; Lenard M, Voortman; Thomas H, Sharp
Published in: Journal of Structural Biology, 2023, ISSN 1047-8477
Publisher: Academic Press
DOI: 10.1016/j.jsb.2023.107965

Plasmodium falciparum has evolved multiple mechanisms to hijack human immunoglobulin M

Author(s): Chenggong Ji, Hao Shen, Chen Su, Yaxin Li, Shihua Chen, Thomas H. Sharp, Junyu Xiao
Published in: Nature Communications, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-38320-z

Correlated Cryo Super‐Resolution Light and Cryo‐Electron Microscopy on Mammalian Cells Expressing the Fluorescent Protein rsEGFP2

Author(s): Maarten W. Tuijtel, Abraham J. Koster, Frank G. A. Faas, Thomas H. Sharp
Published in: Small Methods, Issue 3/12, 2019, Page(s) 1900425, ISSN 2366-9608
Publisher: Wiley
DOI: 10.1002/smtd.201900425

Complement is activated by elevated IgG3 hexameric platforms and deposits C4b onto distinct antibody domains

Author(s): Leoni Abendstein; Douwe J. Dijkstra; Rayman T. N. Tjokrodirijo; Peter A. van Veelen; Leendert A. Trouw; Paul J. Hensbergen; Thomas H. Sharp
Published in: Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023), 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-39788-5