Protein nano-patterning using DNA nanotechnology; control of surface-based immune system activation

Informazioni relative al progetto

BioNanoPattern

ID dell’accordo di sovvenzione: 759517

DOI

10.3030/759517

Progetto chiuso

Data della firma CE 12 Settembre 2017

Data di avvio 1 Gennaio 2018

Data di completamento 31 Dicembre 2023

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 1 499 850,00

Contributo UE

€ 1 499 850,00

Coordinato da

ACADEMISCH ZIEKENHUIS LEIDEN
Netherlands

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (18)

Selecting optimal support grids for super-resolution cryogenic correlated light and electron microscopy

Autori: Mart G. F. Last, Maarten W. Tuijtel, Lenard M. Voortman, Thomas H. Sharp
Pubblicato in: Scientific Reports, 2023, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-023-35590-x

Alternative Polyadenylation Utilization Results in Ribosome Assembly and mRNA Translation Deficiencies in a Model for Muscle Aging

Autori: Hailiang Mei, PhD, Jasper Boom, BSc, Salma el Abdellaoui, BSc, Kotb Abdelmohsen, PhD, Rachel Munk, MSc, Jennifer L Martindale, MSc, Susan Kloet, PhD, Szymone M Kielbasa, PhD, Thomas H Sharp, PhD, Myriam Gorospe, PhD, Vered Raz, PhD
Pubblicato in: The Journals of Gerontology: Series A, 2022, ISSN 1079-5006
Editore: Gerontological Society of America
DOI: 10.1093/gerona/glac058

Cryo-Electron Microscopy and Biochemical Analysis Offer Insights Into the Effects of Acidic pH, Such as Occur During Acidosis, on the Complement Binding Properties of C-Reactive Protein

Autori: Dylan P. Noone, Tijn T. van der Velden and Thomas H. Sharp
Pubblicato in: Frontiers in Immunology, 2021, ISSN 1664-3224
Editore: Frontiers
DOI: 10.3389/fimmu.2021.757633

Human anti-C1q autoantibodies bind specifically to solid-phase C1q and enhance phagocytosis but not complement activation

Autori: Douwe J Dijkstra, Fleur S van de Bovenkamp, Leoni Abendstein, Rob Zuijderduijn, Jos Pool, Cynthia SM Kramer, Linda M Slot, Jan W Drijfhout, Lisanne de Vor, Kyra A Gelderman, Suzan HM Rooijakkers, Arnaud Zaldumbide, Gestur Vidarsson, Thomas H Sharp, Paul WHI Parren, Leendert A Trouw
Pubblicato in: PNAS, 2024, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.2310666120

PTX3 structure determination using a hybrid cryoelectron microscopy and AlphaFold approach offers insights into ligand binding and complement activation

Autori: Dylan P. Noone, Douwe J. Dijkstra, Teun T. van der Klugt https, Peter A. van Veelen, Arnoud H. de Ru, Paul J. Hensbergen, Leendert A. Trouw, and Thomas H. Sharp
Pubblicato in: Proceedings of the National Academy of Sciences, USA, 2022, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.220814411

Super-resolution fluorescence imaging of cryosamples does not limit achievable resolution in cryoEM

Autori: Mart G.F. Last; Willem E.M. Noteborn; Lenard M. Voortman; Thomas H. Sharp
Pubblicato in: Journal of Structural Biology, Numero 2, 2023, ISSN 1047-8477
Editore: Academic Press
DOI: 10.1016/j.jsb.2023.108040

Efficient mRNA delivery using lipid nanoparticles modified with fusogenic coiled-coil peptides

Autori: Ye Zeng, Mengjie Shen, Roy Pattipeiluhu, Xuequan Zhou, Yun Zhang, Thomas Bakkum, Thomas H Sharp, Aimee L Boyle, Alexander Kros
Pubblicato in: Nanoscale, 2023, ISSN 2040-3372
Editore: Royal Society of Chemistry
DOI: 10.1039/d3nr02175k

Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins

Autori: Maarten W. Tuijtel, Abraham J. Koster, Stefan Jakobs, Frank G. A. Faas, Thomas H. Sharp
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-018-37728-8

Carbamylation reduces the capacity of IgG for hexamerization and complement activation

Autori: R. Lubbers, S. C. Oostindie, D. J. Dijkstra, P. W. H. I. Parren, M. K. Verheul, L. Abendstein, T. H. Sharp, A. Ru, G. M. C. Janssen, P. A. Veelen, E. T. J. den Bremer, B. Bleijlevens, B.‐J. Kreuk, F. J. Beurskens, L. A. Trouw
Pubblicato in: Clinical & Experimental Immunology, Numero 200/1, 2020, Pagina/e 1-11, ISSN 0009-9104
Editore: Blackwell Publishing Inc.
DOI: 10.1111/cei.13411

scNodes: a correlation and processing toolkit for super-resolution fluorescence and electron microscopy

Autori: Mart G. F. Last; Lenard M. Voortman; Thomas H. Sharp
Pubblicato in: Nature Methods, 2023, ISSN 1548-7091
Editore: Nature Publishing Group
DOI: 10.1038/s41592-023-01991-z

Insights into IgM-mediated complement activation based on in situ structures of IgM-C1-C4b

Autori: Thomas H. Sharp, Aimee L. Boyle, Christoph A. Diebolder, Alexander Kros, Abraham J. Koster, Piet Gros
Pubblicato in: Proceedings of the National Academy of Sciences, 2019, Pagina/e 201901841, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1901841116

Liquid crystalline inverted lipid phases encapsulating siRNA enhance lipid nanoparticle mediated transfection

Autori: Roy Pattipeiluhu, Ye Zeng, Marco M.R.M. Hendrix, Ilja K. Voets, Alexander Kros, Thomas H. Sharp
Pubblicato in: Nature Communications, 2024, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-024-45666-5

One-Pot Synthesis of Defined-Length ssDNA for Multiscaffold DNA Origami

Autori: Willem E. M. Noteborn, Leoni Abendstein, Thomas H. Sharp
Pubblicato in: Bioconjugate Chemistry, Numero 32/1, 2021, Pagina/e 94-98, ISSN 1043-1802
Editore: American Chemical Society
DOI: 10.1021/acs.bioconjchem.0c00644

Anionic Lipid Nanoparticles Preferentially Deliver mRNA to the Hepatic Reticuloendothelial System

Autori: Roy Pattipeiluhu,Gabriela Arias-Alpizar,Genc Basha,Karen Y. T. Chan,Jeroen Bussmann,Thomas H. Sharp,Mohammad-Amin Moradi,Nico Sommerdijk,Edward N. Harris,Pieter R. Cullis,Alexander Kros,Dominik Witzigmann,Frederick Campbell
Pubblicato in: Advanced Materials, 2022, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202201095

Measuring cryo-TEM sample thickness using reflected light microscopy and machine learning

Autori: Mart G F, Last; Lenard M, Voortman; Thomas H, Sharp
Pubblicato in: Journal of Structural Biology, 2023, ISSN 1047-8477
Editore: Academic Press
DOI: 10.1016/j.jsb.2023.107965

Plasmodium falciparum has evolved multiple mechanisms to hijack human immunoglobulin M

Autori: Chenggong Ji, Hao Shen, Chen Su, Yaxin Li, Shihua Chen, Thomas H. Sharp, Junyu Xiao
Pubblicato in: Nature Communications, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-38320-z

Correlated Cryo Super‐Resolution Light and Cryo‐Electron Microscopy on Mammalian Cells Expressing the Fluorescent Protein rsEGFP2

Autori: Maarten W. Tuijtel, Abraham J. Koster, Frank G. A. Faas, Thomas H. Sharp
Pubblicato in: Small Methods, Numero 3/12, 2019, Pagina/e 1900425, ISSN 2366-9608
Editore: Wiley
DOI: 10.1002/smtd.201900425

Complement is activated by elevated IgG3 hexameric platforms and deposits C4b onto distinct antibody domains

Autori: Leoni Abendstein; Douwe J. Dijkstra; Rayman T. N. Tjokrodirijo; Peter A. van Veelen; Leendert A. Trouw; Paul J. Hensbergen; Thomas H. Sharp
Pubblicato in: Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023), 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-39788-5