Objective
CD8 T cells are vital for our response to infection, directly killing infected cells and stimulating other cells of the immune system. Major health problems world-wide involve deregulated T cells, including lymphomas, leukaemias, auto-immune disorders and organ transplant rejection. Damaging deficiencies of T cells are found in old-age. T cell development and response to pathogens is driven by regulated transcription and translation, however the mechanisms orchestrating this gene regulation are largely unknown, and are likely provide novel opportunities for therapeutic intervention. Our preliminary data reveals that a potent structure in gene expression, the mRNA cap, which co-ordinates RNA processing and translation initiation, is crucial for the development and activation of CD8 T cells. Regulation of mRNA cap formation is a novel concept in gene regulation in T cells, and also has not been studied in vivo previously. Our findings suggest the existence of an mRNA cap code, in which the different mRNA cap methylations regulate different genes and cellular functions. In TCAPS we will reveal how the mRNA cap code orchestrates CD8 T function. We will determine how the mRNA cap structures are regulated during CD8 T cell differentiation using the latest mass spectrometry technologies. We will determine the role of each mRNA cap methyltransferase in CD8 T cell gene expression during differentiation, using conditional knock-out mice in conjunction with enzyme biochemistry, RNA sequencing and quantitative mass spectrometry. We will determine the biological function of the mRNA cap methyltransferases in CD8 T cell survival and activation using immunological assays. TCAPS will also provide the first insight into the role of the mRNA cap code in vivo. The mRNA cap code is likely to be operational throughout mammalian physiology and therefore TCAPS will contribute significantly to our understanding of regulated gene expression in mammals.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine immunology
- medical and health sciences basic medicine physiology
- natural sciences biological sciences genetics RNA
- medical and health sciences clinical medicine oncology leukemia
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
ERC-COG - Consolidator Grant
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2017-COG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
G61 1BD Bearsden
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.