Electrogenetics – Shaping Electrogenetic Interfaces for Closed-Loop Voltage-Controlled Gene Expression

Información del proyecto

ElectroGene

Identificador del acuerdo de subvención: 785800

DOI

10.3030/785800

Proyecto cerrado

Fecha de la firma de la CE 29 Mayo 2018

Fecha de inicio 1 Noviembre 2018

Fecha de finalización 31 Enero 2025

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 2 500 000,00

Aportación de la UE

€ 2 500 000,00

Coordinado por

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (56)

A versatile bioelectronic interface programmed for hormone sensing

Autores: Preetam Guha Ray, Debasis Maity, Jinbo Huang, Henryk Zulewski, Martin Fussenegger
Publicado en: Nature Communications, Edición 14, 2023, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-023-39015-1

An electrogenetic interface to program mammalian gene expression by direct current

Autores: Jinbo Huang, Shuai Xue, Peter Buchmann, Ana Palma Teixeira, Martin Fussenegger
Publicado en: Nature Metabolism, Edición 5, 2023, Página(s) 1395-1407, ISSN 2522-5812
Editor: Nature Springer
DOI: 10.1038/s42255-023-00850-7

A fully human transgene switch to regulate therapeutic protein production by cooling sensation

Autores: Peng Bai, Ying Liu, Shuai Xue, Ghislaine Charpin-El Hamri, Pratik Saxena, Haifeng Ye, Mingqi Xie, Martin Fussenegger
Publicado en: Nature Medicine, Edición 25/8, 2019, Página(s) 1266-1273, ISSN 1078-8956
Editor: Nature Publishing Group
DOI: 10.1038/s41591-019-0501-8

Structural materials meet synthetic biology in biomedical applications

Autores: Xiaoxuan Zhang, Martin Fussenegger
Publicado en: Materials Today, Edición 72, 2024, Página(s) 163-182, ISSN 1369-7021
Editor: Elsevier BV
DOI: 10.1016/j.mattod.2023.12.008

Synthetic macromolecular switches for precision control of therapeutic cell functions

Autores: Ana Palma Teixeira, Martin Fussenegger
Publicado en: Nature Reviews Bioengineering, Edición 2, 2024, Página(s) 1005-1022, ISSN 2731-6092
Editor: Nature Springer
DOI: 10.1038/s44222-024-00235-9

Regulation of Transgene Expression by the Natural Sweetener Xylose

Autores: Galvan, Silvia; Madderson, Oliver; Xue, Shuai; Teixeira, Ana P.; Fussenegger, Martin
Publicado en: Advanced Science, 9 (34), Edición 1, 2023, ISSN 2198-3844
Editor: Wiley-VCH
DOI: 10.1002/advs.202203193

A Gene‐Switch Platform Interfacing with Reactive Oxygen Species Enables Transcription Fine‐Tuning by Soluble and Volatile Pharmacologics and Food Additives

Autores: Jinbo Huang, Shuai Xue, Ana Palma Teixeira, Martin Fussenegger
Publicado en: Advanced Science, Edición 11, 2024, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202306333

Phosphoregulated orthogonal signal transduction in mammalian cells

Autores: Leo Scheller, Marc Schmollack, Adrian Bertschi, Maysam Mansouri, Pratik Saxena, Martin Fussenegger
Publicado en: Nature Communications, Edición 11/1, 2020, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-020-16895-1

Smartphone-Flashlight-Mediated Remote Control of Rapid Insulin Secretion Restores Glucose Homeostasis in Experimental Type-1 Diabetes

Autores: Mansouri, Maysam; Xue, Shuai; Hussherr, Marie-Didiée; Strittmatter, Tobias; Camenisch, Gieri; Fussenegger, Martin
Publicado en: Small, 17 (35), Edición 17, 2021, ISSN 1613-6810
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202202566

Engineering Receptors in the Secretory Pathway for Orthogonal Signalling Control

Autores: Mahameed Mohamed; Wang Pengli; Xue Shuai; Fussenegger Martin
Publicado en: Nature Communications, Edición 1, 2022, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-022-35161-0

Identification of Sclareol As a Natural Neuroprotective Caᵥ1.3-Antagonist Using Synthetic Parkinson-Mimetic Gene Circuits and Computer-Aided Drug Discovery

Autores: Wang, Hui; Xie, Mingqi; Rizzi, Giorgio; Li, Xin; Tan, Kelly; Fussenegger, Martin
Publicado en: Advanced Science, 9 (7), Edición 1, 2022, ISSN 2198-3844
Editor: Wiley-VCH
DOI: 10.1002/advs.202102855

Synthetic biology in multicellular organisms: Opportunities in nematodes

Autores: Dmytro Kukhtar, Martin Fussenegger
Publicado en: Biotechnology and Bioengineering, Edición 120, 2023, Página(s) 2056-2071, ISSN 0006-3592
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28497

Synthetic Gene Circuits for Regulation of Next‐Generation Cell‐Based Therapeutics

Autores: Ana P. Teixeira, Martin Fussenegger
Publicado en: Advanced Science, Edición 11, 2024, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202309088

Building sophisticated sensors of extracellular cues that enable mammalian cells to work as “doctors” in the body

Autores: Ryosuke Kojima, Dominque Aubel, Martin Fussenegger
Publicado en: Cellular and Molecular Life Sciences, Edición 77/18, 2020, Página(s) 3567-3581, ISSN 1420-682X
Editor: Birkhauser Verlag
DOI: 10.1007/s00018-020-03486-y

A modular degron library for synthetic circuits in mammalian cells

Autores: Hélène Chassin, Marius Müller, Marcel Tigges, Leo Scheller, Moritz Lang, Martin Fussenegger
Publicado en: Nature Communications, Edición 10/1, 2019, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-019-09974-5

Synthetic Biology: Engineering Mammalian Cells To Control Cell‐to‐Cell Communication at Will

Autores: Ryosuke Kojima, Martin Fussenegger
Publicado en: ChemBioChem, Edición 20/8, 2019, Página(s) 994-1002, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800682

Programmable DARPin-based receptors for the detection of thrombotic markers

Autores: Tobias Strittmatter; Yidan Wang; Adrian Bertschi; Leo Scheller; Patrick C. Freitag; Preetam Guha Ray; Pascal Stuecheli; Jonas V. Schaefer; Thomas Reinberg; Dimitrios Tsakiris; Andreas Plückthun; Haifeng Ye; Martin Fussenegger
Publicado en: Nature Chemical Biology, 18, Edición 1, 2022, ISSN 1552-4450
Editor: Nature Publishing Group
DOI: 10.1038/s41589-022-01095-3

Electrogenetics: Bridging synthetic biology and electronics to remotely control the behavior of mammalian designer cells

Autores: Maysam Mansouri; Martin Fussenegger
Publicado en: Current Opinion in Chemical Biology, 68, Edición 5, 2022, ISSN 1367-5931
Editor: Elsevier BV
DOI: 10.1016/j.cbpa.2022.102151

Smartphone‐Flashlight‐Mediated Remote Control of Rapid Insulin Secretion Restores Glucose Homeostasis in Experimental Type‐1 Diabetes

Autores: Maysam Mansouri, Shuai Xue, Marie‐Didiée Hussherr, Tobias Strittmatter, Gieri Camenisch, Martin Fussenegger
Publicado en: Small, Edición 17/35, 2021, Página(s) 2101939, ISSN 1613-6810
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202101939

Engineering precision therapies: lessons and motivations from the clinic

Autores: Mingqi Xie, Mirta Viviani, Martin Fussenegger
Publicado en: Synthetic Biology, Edición 6/1, 2021, ISSN 2397-7000
Editor: Oxford Academic Press
DOI: 10.1093/synbio/ysaa024

Glucose‐Operated Widget (GLOW) for Closed‐Loop Optogenetic Glycemic Control

Autores: Debasis Maity, Preetam Guha Ray, Martin Fussenegger
Publicado en: Advanced Materials, Edición 36, 2024, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202408537

Programming mammalian cell behaviors by physical cues

Autores: Jinbo Huang, Martin Fussenegger
Publicado en: Trends in Biotechnology, Edición 43, 2025, Página(s) 16-42, ISSN 0167-7799
Editor: Elsevier BV
DOI: 10.1016/j.tibtech.2024.07.014

Electrogenetic cellular insulin release for real-time glycemic control in type 1 diabetic mice

Autores: Krzysztof Krawczyk, Shuai Xue, Peter Buchmann, Ghislaine Charpin-El-Hamri, Pratik Saxena, Marie-Didiée Hussherr, Jiawei Shao, Haifeng Ye, Mingqi Xie, Martin Fussenegger
Publicado en: Science, Edición 368/6494, 2020, Página(s) 993-1001, ISSN 0036-8075
Editor: American Association for the Advancement of Science
DOI: 10.1126/science.aau7187

Design of programmable post-translational switch control platform for on-demand protein secretion in mammalian cells

Autores: Maysam Mansouri; Preetam Guha Ray; Nik Franko; Shuai Xue; Martin Fussenegger
Publicado en: Nucleic Acids Research, 51 (1), Edición 1, 2023, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkac916

Autonomous push button-controlled rapid insulin release from a piezoelectrically activated subcutaneous cell implant

Autores: Haijie Zhao, Shuai Xue, Marie-Didiée Hussherr, Ana Palma Teixeira, Martin Fussenegger
Publicado en: Science Advances, 2022, ISSN 2375-2548
Editor: American Association for the Advancement of Science
DOI: 10.1126/sciadv.abm4389

From synthetic biology to human therapy: engineered mammalian cells

Autores: Leo Scheller, Martin Fussenegger
Publicado en: Current Opinion in Biotechnology, 2019, ISSN 0958-1669
Editor: Elsevier BV
DOI: 10.1016/j.copbio.2019.02.023

Engineering autonomous closed-loop designer cells for disease therapy.

Autores: Mohamed Mahameed; Martin Fussenegger
Publicado en: iScience, 25 (3), Edición 1, 2022, ISSN 2589-0042
Editor: Cell Press Elsevier
DOI: 10.1016/j.isci.2022.103834

Rational design and optimization of synthetic gene switches for controlling cell-fate decisions in pluripotent stem cells

Autores: Viktor Haellman, Pratik Saxena, Yanrui Jiang, Martin Fussenegger
Publicado en: Metabolic Engineering, Edición 65, 2021, Página(s) 99-110, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2021.03.009

Small‐Molecule Regulators for Gene Switches to Program Mammalian Cell Behaviour

Autores: Maysam Mansouri, Martin Fussenegger
Publicado en: ChemBioChem, Edición 25, 2025, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202300717

Evolution of molecular switches for regulation of transgene expression by clinically licensed gluconate

Autores: Ana Palma Teixeira, Shuai Xue, Jinbo Huang, Martin Fussenegger
Publicado en: Nucleic Acids Research, Edición 51, 2023, Página(s) e85-e85, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkad600

5-Fluorouracil blocks quorum-sensing of biofilm-embedded methicillin-resistant Staphylococcus aureus in mice

Autores: Ferdinand Sedlmayer, Anne-Kathrin Woischnig, Vincent Unterreiner, Florian Fuchs, Daniel Baeschlin, Nina Khanna, Martin Fussenegger
Publicado en: Nucleic Acids Research, 2021, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkab251

Integrated compact regulators of protein activity enable control of signaling pathways and genome-editing in vivo

Autores: Nik Franko, António José da Silva Santinha, Shuai Xue, Haijie Zhao, Ghislaine Charpin-El Hamri, Randall Jeffrey Platt, Ana Palma Teixeira, Martin Fussenegger
Publicado en: Cell Discovery, Edición 10, 2024, ISSN 2056-5968
Editor: Nature Springer
DOI: 10.1038/s41421-023-00632-1

Genetically Encoded Protein Thermometer Enables Precise Electrothermal Control of Transgene Expression

Autores: Bozhidar‐Adrian Stefanov, Ana P. Teixeira, Maysam Mansouri, Adrian Bertschi, Krzysztof Krawczyk, Ghislaine Charpin‐El Hamri, Shuai Xue, Martin Fussenegger
Publicado en: Advanced Science, Edición 8/21, 2021, Página(s) 2101813, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202101813

Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins

Autores: Mohamed Mahameed, Shuai Xue, Benjamin Danuser, Ghislaine Charpin-El Hamri, Mingqi Xie, Martin Fussenegger
Publicado en: Nature Biomedical Engineering, 2025, ISSN 2157-846X
Editor: Nature Springer
DOI: 10.1038/s41551-025-01350-7

Engineering electrogenetic interfaces for mammalian cell control

Autores: Maysam Mansouri, Martin Fussenegger
Publicado en: Cell Chemical Biology, 2025, ISSN 2451-9456
Editor: Elsevier
DOI: 10.1016/j.chembiol.2025.01.003

The Role of Protein Engineering in Biomedical Applications of Mammalian Synthetic Biology

Autores: Bojar, Daniel; Fussenegger, Martin; id_orcid0000-0001-8545-667X
Publicado en: Small, 16 (27), Edición 1, 2020, ISSN 1613-6810
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.201903093

A versatile plasmid architecture for mammalian synthetic biology (VAMSyB)

Autores: Viktor Haellman, Tobias Strittmatter, Adrian Bertschi, Pascal Stücheli, Martin Fussenegger
Publicado en: Metabolic Engineering, Edición 66, 2021, Página(s) 41-50, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2021.04.003

Combinatorial protein dimerization enables precise multi-input synthetic computations

Autores: Adrian Bertschi, Pengli Wang, Silvia Galvan, Ana Palma Teixeira, Martin Fussenegger
Publicado en: Nature Chemical Biology, 2023, ISSN 1552-4450
Editor: Nature Publishing Group
DOI: 10.1038/s41589-023-01281-x

Toward Photosynthetic Mammalian Cells through Artificial Endosymbiosis

Autores: Guipeng Hu, Jinbo Huang, Martin Fussenegger
Publicado en: Small, Edición 20, 2024, ISSN 1613-6810
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202310310

CelloSelect - A synthetic cellobiose metabolic pathway for selection of stable transgenic CHO cell lines

Autores: Ana P Teixeira, Pascal Stücheli, Simon Ausländer, David Ausländer, Pascal Schönenberger, Samuel Hürlemann, Martin Fussenegger
Publicado en: Metabolic Engineering, 2022, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2022.01.001

Smart-watch-programmed green-light-operated percutaneous control of therapeutic transgenes

Autores: Maysam Mansouri, Marie-Didiée Hussherr, Tobias Strittmatter, Peter Buchmann, Shuai Xue, Gieri Camenisch, Martin Fussenegger
Publicado en: Nature Communications, Edición 12/1, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-23572-4

Shedding Light on Extracellular Vesicle Biogenesis and Bioengineering

Autores: Fei Teng, Martin Fussenegger
Publicado en: Advanced Science, Edición 8/1, 2021, Página(s) 2003505, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202003505

Synthetic biology-based optogenetic approaches to control therapeutic designer cells

Autores: Maysam Mansouri, Martin Fussenegger
Publicado en: Current Opinion in Systems Biology, Edición 28, 2021, Página(s) 100396, ISSN 2452-3100
Editor: Elsevier BV
DOI: 10.1016/j.coisb.2021.100396

dCas9-mediated dysregulation of gene expression in human induced pluripotent stem cells during primitive streak differentiation

Autores: Viktor Haellman; Martin Pirkl; Arslan Akmammedov; Pratik Saxena; Niko Beerenwinkel; Renato Paro; Ana Palma Teixeira; Martin Fussenegger
Publicado en: Metabolic Engineering, 73, Edición 1, 2022, ISSN 1096-7176
Editor: Academic Press
DOI: 10.3929/ethz-b-000557172

Blood-Glucose-Powered Metabolic Fuel Cell for Self-Sufficient Bioelectronics

Autores: Debasis Maity, Preetam Guha Ray, Peter Buchmann, Maysam Mansouri, Martin Fussenegger
Publicado en: Advanced Materials, 2023, ISSN 1521-4095
Editor: Wiley-VCH
DOI: 10.1002/adma.202300890

Controlling therapeutic protein expression via inhalation of a butter flavor molecule

Autores: Adrian Bertschi; Bozhidar-Adrian Stefanov; Shuai Xue; Ghislaine Charpin-El Hamri; Ana Palma Teixeira; Martin Fussenegger
Publicado en: Crossref, Edición 1, 2023, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkac1256

Ultrashort‐Peptide‐Responsive Gene Switches for Regulation of Therapeutic Protein Expression in Mammalian Cells

Autores: Jinbo Huang, Shuai Xue, Yu‐Qing Xie, Ana Palma Teixeira, Martin Fussenegger
Publicado en: Advanced Science, Edición 11, 2024, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202309411

Engineering a Rapid Insulin Release System Controlled By Oral Drug Administration

Autores: Mahameed, Mohamed; Xue, Shuai; Stefanov, Bozhidar-Adrian; Charpin-El Hamri, Ghislaine; Fussenegger, Martin; id_orcid0000-0001-8545-667X
Publicado en: Advanced Science, 9 (9), Edición 1, 2022, ISSN 2198-3844
Editor: Wiley-VCH
DOI: 10.1002/advs.202105619

Biomarker-driven feedback control of synthetic biology systems for next-generation personalized medicine

Autores: Stefanov, Bozhidar-Adrian; Fussenegger, Martin; id_orcid0000-0001-8545-667X
Publicado en: Frontiers in Bioengineering and Biotechnology, 10, Edición 1, 2022, ISSN 2296-4185
Editor: Frontier Media
DOI: 10.3389/fbioe.2022.986210

Skin‐Integrated Electrogenetic Regulation of Vasculature for Accelerated Wound Healing

Autores: Preetam Guha Ray, Ragavi Rajasekaran, Bitan Pratihar, Sirshendu De, Santanu Dhara, Martin Fussenegger
Publicado en: Advanced Science, 2025, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202412257

Therapeutic cell engineering: designing programmable synthetic genetic circuits in mammalian cells

Autores: Mansouri, Maysam; Fussenegger, Martin; id_orcid0000-0001-8545-667X
Publicado en: Protein & Cell, 13 (7), Edición 1, 2022, ISSN 1674-800X
Editor: Springer Verlag
DOI: 10.1007/s13238-021-00876-1

The sound of silence: Transgene silencing in mammalian cell engineering

Autores: Alan Cabrera, Hailey I Edelstein, Fokion Glykofrydis, Kasey S Love, Sebastian Palacios, Josh Tycko, Meng Zhang, Sarah Lensch, Cara E Shields, Mark Livingston, Ron Weiss, Huimin Zhao, Karmella A Haynes, Leonardo Morsut, Yvonne Y Chen, Ahmad S Khalil, Wilson W Wong, James J Collins, Susan J Rosser, Karen Polizzi, Michael B Elowitz, Martin Fussenegger, Isaac B Hilton, Joshua N Leonard, Lacramioara Bi
Publicado en: Cell Systems, 2022, ISSN 2405-4712
Editor: Elsevier
DOI: 10.1016/j.cels.2022.11.005

Hydratable Core–Shell Polymer Networks for Atmospheric Water Harvesting Powered by Sunlight

Autores: Debasis Maity, Ana Palma Teixeira, Martin Fussenegger
Publicado en: Small, Edición 19, 2023, ISSN 1613-6810
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202301427

An Efficient Ambient-Moisture–Driven Wearable Electrical Power Generator

Autores: Debasis Maity, Martin Fussenegger
Publicado en: Advanced Science, 2023, ISSN 2198-3844
Editor: Wiley-VCH
DOI: 10.1002/advs.202300750

Enhancing cell‐based therapies with synthetic gene circuits responsive to molecular stimuli

Autores: Silvia Galvan, Ana P. Teixeira, Martin Fussenegger
Publicado en: Biotechnology and Bioengineering, Edición 121, 2024, Página(s) 2987-3000, ISSN 0006-3592
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28770

Emerging mammalian gene switches for controlling implantable cell therapies

Autores: Oliver Madderson, Ana Palma Teixeira, Martin Fussenegger
Publicado en: Current Opinion in Chemical Biology, Edición 64, 2021, Página(s) 98-105, ISSN 1367-5931
Editor: Elsevier BV
DOI: 10.1016/j.cbpa.2021.05.012

Book chapters (2)

Construction of a Multiwell Light-Induction Platform for Traceless Control of Gene Expression in Mammalian Cells.

Autores: Maysam Mansouri; Samson Lichtenstein; Tobias Strittmatter; Peter Buchmann; Martin Fussenegger; Martin Fussenegger
Publicado en: Methods in Molecular Biology - Photoswitching Proteins, Edición 1, 2020
Editor: Springer
DOI: 10.1007/978-1-0716-0755-8_13

Posttranslational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells

Autores: Maysam Mansouri, Martin Fussenegger
Publicado en: Methods in Molecular Biology, Mammalian Synthetic Systems, 2024, Página(s) 233-241
Editor: Springer US
DOI: 10.1007/978-1-0716-3718-0_15