Electrogenetics – Shaping Electrogenetic Interfaces for Closed-Loop Voltage-Controlled Gene Expression

Informazioni relative al progetto

ElectroGene

ID dell’accordo di sovvenzione: 785800

DOI

10.3030/785800

Progetto chiuso

Data della firma CE 29 Maggio 2018

Data di avvio 1 Novembre 2018

Data di completamento 31 Gennaio 2025

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 2 500 000,00

Contributo UE

€ 2 500 000,00

Coordinato da

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (56)

A versatile bioelectronic interface programmed for hormone sensing

Autori: Preetam Guha Ray, Debasis Maity, Jinbo Huang, Henryk Zulewski, Martin Fussenegger
Pubblicato in: Nature Communications, Numero 14, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-39015-1

An electrogenetic interface to program mammalian gene expression by direct current

Autori: Jinbo Huang, Shuai Xue, Peter Buchmann, Ana Palma Teixeira, Martin Fussenegger
Pubblicato in: Nature Metabolism, Numero 5, 2023, Pagina/e 1395-1407, ISSN 2522-5812
Editore: Nature Springer
DOI: 10.1038/s42255-023-00850-7

A fully human transgene switch to regulate therapeutic protein production by cooling sensation

Autori: Peng Bai, Ying Liu, Shuai Xue, Ghislaine Charpin-El Hamri, Pratik Saxena, Haifeng Ye, Mingqi Xie, Martin Fussenegger
Pubblicato in: Nature Medicine, Numero 25/8, 2019, Pagina/e 1266-1273, ISSN 1078-8956
Editore: Nature Publishing Group
DOI: 10.1038/s41591-019-0501-8

Structural materials meet synthetic biology in biomedical applications

Autori: Xiaoxuan Zhang, Martin Fussenegger
Pubblicato in: Materials Today, Numero 72, 2024, Pagina/e 163-182, ISSN 1369-7021
Editore: Elsevier BV
DOI: 10.1016/j.mattod.2023.12.008

Synthetic macromolecular switches for precision control of therapeutic cell functions

Autori: Ana Palma Teixeira, Martin Fussenegger
Pubblicato in: Nature Reviews Bioengineering, Numero 2, 2024, Pagina/e 1005-1022, ISSN 2731-6092
Editore: Nature Springer
DOI: 10.1038/s44222-024-00235-9

Regulation of Transgene Expression by the Natural Sweetener Xylose

Autori: Galvan, Silvia; Madderson, Oliver; Xue, Shuai; Teixeira, Ana P.; Fussenegger, Martin
Pubblicato in: Advanced Science, 9 (34), Numero 1, 2023, ISSN 2198-3844
Editore: Wiley-VCH
DOI: 10.1002/advs.202203193

A Gene‐Switch Platform Interfacing with Reactive Oxygen Species Enables Transcription Fine‐Tuning by Soluble and Volatile Pharmacologics and Food Additives

Autori: Jinbo Huang, Shuai Xue, Ana Palma Teixeira, Martin Fussenegger
Pubblicato in: Advanced Science, Numero 11, 2024, ISSN 2198-3844
Editore: Wiley
DOI: 10.1002/advs.202306333

Phosphoregulated orthogonal signal transduction in mammalian cells

Autori: Leo Scheller, Marc Schmollack, Adrian Bertschi, Maysam Mansouri, Pratik Saxena, Martin Fussenegger
Pubblicato in: Nature Communications, Numero 11/1, 2020, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-020-16895-1

Smartphone-Flashlight-Mediated Remote Control of Rapid Insulin Secretion Restores Glucose Homeostasis in Experimental Type-1 Diabetes

Autori: Mansouri, Maysam; Xue, Shuai; Hussherr, Marie-Didiée; Strittmatter, Tobias; Camenisch, Gieri; Fussenegger, Martin
Pubblicato in: Small, 17 (35), Numero 17, 2021, ISSN 1613-6810
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202202566

Engineering Receptors in the Secretory Pathway for Orthogonal Signalling Control

Autori: Mahameed Mohamed; Wang Pengli; Xue Shuai; Fussenegger Martin
Pubblicato in: Nature Communications, Numero 1, 2022, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-35161-0

Identification of Sclareol As a Natural Neuroprotective Caᵥ1.3-Antagonist Using Synthetic Parkinson-Mimetic Gene Circuits and Computer-Aided Drug Discovery

Autori: Wang, Hui; Xie, Mingqi; Rizzi, Giorgio; Li, Xin; Tan, Kelly; Fussenegger, Martin
Pubblicato in: Advanced Science, 9 (7), Numero 1, 2022, ISSN 2198-3844
Editore: Wiley-VCH
DOI: 10.1002/advs.202102855

Synthetic biology in multicellular organisms: Opportunities in nematodes

Autori: Dmytro Kukhtar, Martin Fussenegger
Pubblicato in: Biotechnology and Bioengineering, Numero 120, 2023, Pagina/e 2056-2071, ISSN 0006-3592
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28497

Synthetic Gene Circuits for Regulation of Next‐Generation Cell‐Based Therapeutics

Autori: Ana P. Teixeira, Martin Fussenegger
Pubblicato in: Advanced Science, Numero 11, 2024, ISSN 2198-3844
Editore: Wiley
DOI: 10.1002/advs.202309088

Building sophisticated sensors of extracellular cues that enable mammalian cells to work as “doctors” in the body

Autori: Ryosuke Kojima, Dominque Aubel, Martin Fussenegger
Pubblicato in: Cellular and Molecular Life Sciences, Numero 77/18, 2020, Pagina/e 3567-3581, ISSN 1420-682X
Editore: Birkhauser Verlag
DOI: 10.1007/s00018-020-03486-y

A modular degron library for synthetic circuits in mammalian cells

Autori: Hélène Chassin, Marius Müller, Marcel Tigges, Leo Scheller, Moritz Lang, Martin Fussenegger
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-09974-5

Synthetic Biology: Engineering Mammalian Cells To Control Cell‐to‐Cell Communication at Will

Autori: Ryosuke Kojima, Martin Fussenegger
Pubblicato in: ChemBioChem, Numero 20/8, 2019, Pagina/e 994-1002, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800682

Programmable DARPin-based receptors for the detection of thrombotic markers

Autori: Tobias Strittmatter; Yidan Wang; Adrian Bertschi; Leo Scheller; Patrick C. Freitag; Preetam Guha Ray; Pascal Stuecheli; Jonas V. Schaefer; Thomas Reinberg; Dimitrios Tsakiris; Andreas Plückthun; Haifeng Ye; Martin Fussenegger
Pubblicato in: Nature Chemical Biology, 18, Numero 1, 2022, ISSN 1552-4450
Editore: Nature Publishing Group
DOI: 10.1038/s41589-022-01095-3

Electrogenetics: Bridging synthetic biology and electronics to remotely control the behavior of mammalian designer cells

Autori: Maysam Mansouri; Martin Fussenegger
Pubblicato in: Current Opinion in Chemical Biology, 68, Numero 5, 2022, ISSN 1367-5931
Editore: Elsevier BV
DOI: 10.1016/j.cbpa.2022.102151

Smartphone‐Flashlight‐Mediated Remote Control of Rapid Insulin Secretion Restores Glucose Homeostasis in Experimental Type‐1 Diabetes

Autori: Maysam Mansouri, Shuai Xue, Marie‐Didiée Hussherr, Tobias Strittmatter, Gieri Camenisch, Martin Fussenegger
Pubblicato in: Small, Numero 17/35, 2021, Pagina/e 2101939, ISSN 1613-6810
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202101939

Engineering precision therapies: lessons and motivations from the clinic

Autori: Mingqi Xie, Mirta Viviani, Martin Fussenegger
Pubblicato in: Synthetic Biology, Numero 6/1, 2021, ISSN 2397-7000
Editore: Oxford Academic Press
DOI: 10.1093/synbio/ysaa024

Glucose‐Operated Widget (GLOW) for Closed‐Loop Optogenetic Glycemic Control

Autori: Debasis Maity, Preetam Guha Ray, Martin Fussenegger
Pubblicato in: Advanced Materials, Numero 36, 2024, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202408537

Programming mammalian cell behaviors by physical cues

Autori: Jinbo Huang, Martin Fussenegger
Pubblicato in: Trends in Biotechnology, Numero 43, 2025, Pagina/e 16-42, ISSN 0167-7799
Editore: Elsevier BV
DOI: 10.1016/j.tibtech.2024.07.014

Electrogenetic cellular insulin release for real-time glycemic control in type 1 diabetic mice

Autori: Krzysztof Krawczyk, Shuai Xue, Peter Buchmann, Ghislaine Charpin-El-Hamri, Pratik Saxena, Marie-Didiée Hussherr, Jiawei Shao, Haifeng Ye, Mingqi Xie, Martin Fussenegger
Pubblicato in: Science, Numero 368/6494, 2020, Pagina/e 993-1001, ISSN 0036-8075
Editore: American Association for the Advancement of Science
DOI: 10.1126/science.aau7187

Design of programmable post-translational switch control platform for on-demand protein secretion in mammalian cells

Autori: Maysam Mansouri; Preetam Guha Ray; Nik Franko; Shuai Xue; Martin Fussenegger
Pubblicato in: Nucleic Acids Research, 51 (1), Numero 1, 2023, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkac916

Autonomous push button-controlled rapid insulin release from a piezoelectrically activated subcutaneous cell implant

Autori: Haijie Zhao, Shuai Xue, Marie-Didiée Hussherr, Ana Palma Teixeira, Martin Fussenegger
Pubblicato in: Science Advances, 2022, ISSN 2375-2548
Editore: American Association for the Advancement of Science
DOI: 10.1126/sciadv.abm4389

From synthetic biology to human therapy: engineered mammalian cells

Autori: Leo Scheller, Martin Fussenegger
Pubblicato in: Current Opinion in Biotechnology, 2019, ISSN 0958-1669
Editore: Elsevier BV
DOI: 10.1016/j.copbio.2019.02.023

Engineering autonomous closed-loop designer cells for disease therapy.

Autori: Mohamed Mahameed; Martin Fussenegger
Pubblicato in: iScience, 25 (3), Numero 1, 2022, ISSN 2589-0042
Editore: Cell Press Elsevier
DOI: 10.1016/j.isci.2022.103834

Rational design and optimization of synthetic gene switches for controlling cell-fate decisions in pluripotent stem cells

Autori: Viktor Haellman, Pratik Saxena, Yanrui Jiang, Martin Fussenegger
Pubblicato in: Metabolic Engineering, Numero 65, 2021, Pagina/e 99-110, ISSN 1096-7176
Editore: Academic Press
DOI: 10.1016/j.ymben.2021.03.009

Small‐Molecule Regulators for Gene Switches to Program Mammalian Cell Behaviour

Autori: Maysam Mansouri, Martin Fussenegger
Pubblicato in: ChemBioChem, Numero 25, 2025, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202300717

Evolution of molecular switches for regulation of transgene expression by clinically licensed gluconate

Autori: Ana Palma Teixeira, Shuai Xue, Jinbo Huang, Martin Fussenegger
Pubblicato in: Nucleic Acids Research, Numero 51, 2023, Pagina/e e85-e85, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkad600

5-Fluorouracil blocks quorum-sensing of biofilm-embedded methicillin-resistant Staphylococcus aureus in mice

Autori: Ferdinand Sedlmayer, Anne-Kathrin Woischnig, Vincent Unterreiner, Florian Fuchs, Daniel Baeschlin, Nina Khanna, Martin Fussenegger
Pubblicato in: Nucleic Acids Research, 2021, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkab251

Integrated compact regulators of protein activity enable control of signaling pathways and genome-editing in vivo

Autori: Nik Franko, António José da Silva Santinha, Shuai Xue, Haijie Zhao, Ghislaine Charpin-El Hamri, Randall Jeffrey Platt, Ana Palma Teixeira, Martin Fussenegger
Pubblicato in: Cell Discovery, Numero 10, 2024, ISSN 2056-5968
Editore: Nature Springer
DOI: 10.1038/s41421-023-00632-1

Genetically Encoded Protein Thermometer Enables Precise Electrothermal Control of Transgene Expression

Autori: Bozhidar‐Adrian Stefanov, Ana P. Teixeira, Maysam Mansouri, Adrian Bertschi, Krzysztof Krawczyk, Ghislaine Charpin‐El Hamri, Shuai Xue, Martin Fussenegger
Pubblicato in: Advanced Science, Numero 8/21, 2021, Pagina/e 2101813, ISSN 2198-3844
Editore: Wiley
DOI: 10.1002/advs.202101813

Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins

Autori: Mohamed Mahameed, Shuai Xue, Benjamin Danuser, Ghislaine Charpin-El Hamri, Mingqi Xie, Martin Fussenegger
Pubblicato in: Nature Biomedical Engineering, 2025, ISSN 2157-846X
Editore: Nature Springer
DOI: 10.1038/s41551-025-01350-7

Engineering electrogenetic interfaces for mammalian cell control

Autori: Maysam Mansouri, Martin Fussenegger
Pubblicato in: Cell Chemical Biology, 2025, ISSN 2451-9456
Editore: Elsevier
DOI: 10.1016/j.chembiol.2025.01.003

The Role of Protein Engineering in Biomedical Applications of Mammalian Synthetic Biology

Autori: Bojar, Daniel; Fussenegger, Martin; id_orcid0000-0001-8545-667X
Pubblicato in: Small, 16 (27), Numero 1, 2020, ISSN 1613-6810
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.201903093

A versatile plasmid architecture for mammalian synthetic biology (VAMSyB)

Autori: Viktor Haellman, Tobias Strittmatter, Adrian Bertschi, Pascal Stücheli, Martin Fussenegger
Pubblicato in: Metabolic Engineering, Numero 66, 2021, Pagina/e 41-50, ISSN 1096-7176
Editore: Academic Press
DOI: 10.1016/j.ymben.2021.04.003

Combinatorial protein dimerization enables precise multi-input synthetic computations

Autori: Adrian Bertschi, Pengli Wang, Silvia Galvan, Ana Palma Teixeira, Martin Fussenegger
Pubblicato in: Nature Chemical Biology, 2023, ISSN 1552-4450
Editore: Nature Publishing Group
DOI: 10.1038/s41589-023-01281-x

Toward Photosynthetic Mammalian Cells through Artificial Endosymbiosis

Autori: Guipeng Hu, Jinbo Huang, Martin Fussenegger
Pubblicato in: Small, Numero 20, 2024, ISSN 1613-6810
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202310310

CelloSelect - A synthetic cellobiose metabolic pathway for selection of stable transgenic CHO cell lines

Autori: Ana P Teixeira, Pascal Stücheli, Simon Ausländer, David Ausländer, Pascal Schönenberger, Samuel Hürlemann, Martin Fussenegger
Pubblicato in: Metabolic Engineering, 2022, ISSN 1096-7176
Editore: Academic Press
DOI: 10.1016/j.ymben.2022.01.001

Smart-watch-programmed green-light-operated percutaneous control of therapeutic transgenes

Autori: Maysam Mansouri, Marie-Didiée Hussherr, Tobias Strittmatter, Peter Buchmann, Shuai Xue, Gieri Camenisch, Martin Fussenegger
Pubblicato in: Nature Communications, Numero 12/1, 2021, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-021-23572-4

Shedding Light on Extracellular Vesicle Biogenesis and Bioengineering

Autori: Fei Teng, Martin Fussenegger
Pubblicato in: Advanced Science, Numero 8/1, 2021, Pagina/e 2003505, ISSN 2198-3844
Editore: Wiley
DOI: 10.1002/advs.202003505

Synthetic biology-based optogenetic approaches to control therapeutic designer cells

Autori: Maysam Mansouri, Martin Fussenegger
Pubblicato in: Current Opinion in Systems Biology, Numero 28, 2021, Pagina/e 100396, ISSN 2452-3100
Editore: Elsevier BV
DOI: 10.1016/j.coisb.2021.100396

dCas9-mediated dysregulation of gene expression in human induced pluripotent stem cells during primitive streak differentiation

Autori: Viktor Haellman; Martin Pirkl; Arslan Akmammedov; Pratik Saxena; Niko Beerenwinkel; Renato Paro; Ana Palma Teixeira; Martin Fussenegger
Pubblicato in: Metabolic Engineering, 73, Numero 1, 2022, ISSN 1096-7176
Editore: Academic Press
DOI: 10.3929/ethz-b-000557172

Blood-Glucose-Powered Metabolic Fuel Cell for Self-Sufficient Bioelectronics

Autori: Debasis Maity, Preetam Guha Ray, Peter Buchmann, Maysam Mansouri, Martin Fussenegger
Pubblicato in: Advanced Materials, 2023, ISSN 1521-4095
Editore: Wiley-VCH
DOI: 10.1002/adma.202300890

Controlling therapeutic protein expression via inhalation of a butter flavor molecule

Autori: Adrian Bertschi; Bozhidar-Adrian Stefanov; Shuai Xue; Ghislaine Charpin-El Hamri; Ana Palma Teixeira; Martin Fussenegger
Pubblicato in: Crossref, Numero 1, 2023, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkac1256

Ultrashort‐Peptide‐Responsive Gene Switches for Regulation of Therapeutic Protein Expression in Mammalian Cells

Autori: Jinbo Huang, Shuai Xue, Yu‐Qing Xie, Ana Palma Teixeira, Martin Fussenegger
Pubblicato in: Advanced Science, Numero 11, 2024, ISSN 2198-3844
Editore: Wiley
DOI: 10.1002/advs.202309411

Engineering a Rapid Insulin Release System Controlled By Oral Drug Administration

Autori: Mahameed, Mohamed; Xue, Shuai; Stefanov, Bozhidar-Adrian; Charpin-El Hamri, Ghislaine; Fussenegger, Martin; id_orcid0000-0001-8545-667X
Pubblicato in: Advanced Science, 9 (9), Numero 1, 2022, ISSN 2198-3844
Editore: Wiley-VCH
DOI: 10.1002/advs.202105619

Biomarker-driven feedback control of synthetic biology systems for next-generation personalized medicine

Autori: Stefanov, Bozhidar-Adrian; Fussenegger, Martin; id_orcid0000-0001-8545-667X
Pubblicato in: Frontiers in Bioengineering and Biotechnology, 10, Numero 1, 2022, ISSN 2296-4185
Editore: Frontier Media
DOI: 10.3389/fbioe.2022.986210

Skin‐Integrated Electrogenetic Regulation of Vasculature for Accelerated Wound Healing

Autori: Preetam Guha Ray, Ragavi Rajasekaran, Bitan Pratihar, Sirshendu De, Santanu Dhara, Martin Fussenegger
Pubblicato in: Advanced Science, 2025, ISSN 2198-3844
Editore: Wiley
DOI: 10.1002/advs.202412257

Therapeutic cell engineering: designing programmable synthetic genetic circuits in mammalian cells

Autori: Mansouri, Maysam; Fussenegger, Martin; id_orcid0000-0001-8545-667X
Pubblicato in: Protein & Cell, 13 (7), Numero 1, 2022, ISSN 1674-800X
Editore: Springer Verlag
DOI: 10.1007/s13238-021-00876-1

The sound of silence: Transgene silencing in mammalian cell engineering

Autori: Alan Cabrera, Hailey I Edelstein, Fokion Glykofrydis, Kasey S Love, Sebastian Palacios, Josh Tycko, Meng Zhang, Sarah Lensch, Cara E Shields, Mark Livingston, Ron Weiss, Huimin Zhao, Karmella A Haynes, Leonardo Morsut, Yvonne Y Chen, Ahmad S Khalil, Wilson W Wong, James J Collins, Susan J Rosser, Karen Polizzi, Michael B Elowitz, Martin Fussenegger, Isaac B Hilton, Joshua N Leonard, Lacramioara Bi
Pubblicato in: Cell Systems, 2022, ISSN 2405-4712
Editore: Elsevier
DOI: 10.1016/j.cels.2022.11.005

Hydratable Core–Shell Polymer Networks for Atmospheric Water Harvesting Powered by Sunlight

Autori: Debasis Maity, Ana Palma Teixeira, Martin Fussenegger
Pubblicato in: Small, Numero 19, 2023, ISSN 1613-6810
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202301427

An Efficient Ambient-Moisture–Driven Wearable Electrical Power Generator

Autori: Debasis Maity, Martin Fussenegger
Pubblicato in: Advanced Science, 2023, ISSN 2198-3844
Editore: Wiley-VCH
DOI: 10.1002/advs.202300750

Enhancing cell‐based therapies with synthetic gene circuits responsive to molecular stimuli

Autori: Silvia Galvan, Ana P. Teixeira, Martin Fussenegger
Pubblicato in: Biotechnology and Bioengineering, Numero 121, 2024, Pagina/e 2987-3000, ISSN 0006-3592
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28770

Emerging mammalian gene switches for controlling implantable cell therapies

Autori: Oliver Madderson, Ana Palma Teixeira, Martin Fussenegger
Pubblicato in: Current Opinion in Chemical Biology, Numero 64, 2021, Pagina/e 98-105, ISSN 1367-5931
Editore: Elsevier BV
DOI: 10.1016/j.cbpa.2021.05.012

Book chapters (2)

Construction of a Multiwell Light-Induction Platform for Traceless Control of Gene Expression in Mammalian Cells.

Autori: Maysam Mansouri; Samson Lichtenstein; Tobias Strittmatter; Peter Buchmann; Martin Fussenegger; Martin Fussenegger
Pubblicato in: Methods in Molecular Biology - Photoswitching Proteins, Numero 1, 2020
Editore: Springer
DOI: 10.1007/978-1-0716-0755-8_13

Posttranslational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells

Autori: Maysam Mansouri, Martin Fussenegger
Pubblicato in: Methods in Molecular Biology, Mammalian Synthetic Systems, 2024, Pagina/e 233-241
Editore: Springer US
DOI: 10.1007/978-1-0716-3718-0_15