Deep BIOmodeling of human CARDiogenesis

Project Information

BIOCARD

Grant agreement ID: 788381

DOI 10.3030/788381

Project closed

EC signature date 7 August 2018

Start date 1 September 2018

End date 29 February 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 285 625,00

EU contribution € 2 285 625,00

Coordinated by

KLINIKUM RECHTS DER ISAR DER TECHNISCHEN UNIVERSITAT MUNCHEN
Germany

Publications

Peer reviewed articles (27)

Migratory and anti-fibrotic programmes define the regenerative potential of human cardiac progenitors

Author(s): Christine Schneider, Kylie S. Foo, Maria Teresa De Angelis, Gianluca Santamaria, Franziska Reiter, Tatjana Dorn, Andrea Bähr, Yat Long Tsoi, Petra Hoppmann, Ilaria My, Anna Meier, Victoria Jurisch, Nadja Hornaschewitz, Sascha Schwarz, Kun Lu, Roland Tomasi, Stefanie Sudhop, Elvira Parrotta, Marco Gaspari, Giovanni Cuda, Nikolai Klymiuk, Andreas Dendorfer, Markus Krane, Christian Kupatt, Daniel Si
Published in: Nature Cell Biology, Issue 24, 2022, Page(s) 659–671, ISSN 1465-7392
Publisher: Nature Publishing Group
DOI: 10.1038/s41556-022-00899-8

Domain zipping and unzipping modulates TRPM4’s properties in human cardiac conduction disease

Author(s): Wenying Xian, Hongmei Wang, Alessandra Moretti, Karl‐Ludwig Laugwitz, Veit Flockerzi, Peter Lipp
Published in: The FASEB Journal, Issue 34/9, 2020, Page(s) 12114-12126, ISSN 0892-6638
Publisher: Federation of American Societies for Experimental Biology
DOI: 10.1096/fj.202000097rr

Recapitulating porcine cardiac development in vitro: from expanded potential stem cell to embryo culture models

Author(s): Hilansi Rawat, Jessica Kornherr, Dorota Zawada, Sara Bakhshiyeva, Christian Kupatt, Karl-Ludwig Laugwitz, Andrea Bähr, Tatjana Dorn, Alessandra Moretti, Monika Nowak-Imialek
Published in: Frontiers in Cell and Developmental Biology, Issue 11, 2023, Page(s) 1111684, ISSN 2296-634X
Publisher: Frontiers Media S.A.
DOI: 10.3389/fcell.2023.1111684

Precise Correction of Heterozygous SHOX2 Mutations in hiPSCs Derived from Patients with Atrial Fibrillation via Genome Editing and Sib Selection

Author(s): Simon Alexander Sumer, Sandra Hoffmann, Svenja Laue, Birgit Campbell, Kristin Raedecke, Viktoria Frajs, Sebastian Clauss, Stefan Kääb, Johannes W.G. Janssen, Anna Jauch, Karl-Ludwig Laugwitz, Tatjana Dorn, Alessandra Moretti, Gudrun A. Rappold
Published in: Stem Cell Reports, Issue 15/4, 2020, Page(s) 999-1013, ISSN 2213-6711
Publisher: Cell Press
DOI: 10.1016/j.stemcr.2020.08.015

Retinoic acid signaling modulation guides in vitro specification of human heart field-specific progenitor pools

Author(s): Zawada D, Kornherr J, Meier AB, Santamaria G, Dorn T, Nowak-Imialek M, Ortmann D, Zhang F, Lachmann M, Dreßen M, Ortiz M, Mascetti VL, Harmer SC, Nobles M, Tinker A, De Angelis MT, Pedersen RA, Grote P, Laugwitz KL, Moretti A, Goedel A
Published in: Nature communications, 2023, Page(s) 1722, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-36764-x

Muscarinic receptors promote pacemaker fate at the expense of secondary conduction system tissue in zebrafish

Author(s): Martina S. Burczyk, Martin D. Burkhalter, Teresa Casar Tena, Laurel A. Grisanti, Michael Kauk, Sabrina Matysik, Cornelia Donow, Monika Kustermann, Melanie Rothe, Yinghong Cui, Farah Raad, Svenja Laue, Allessandra Moretti, Wolfram-H. Zimmermann, Jürgen Wess, Michael Kühl, Carsten Hoffmann, Douglas G. Tilley, Melanie Philipp
Published in: JCI Insight, Issue 4/20, 2019, ISSN 2379-3708
Publisher: The American Society for Clinical Investigation
DOI: 10.1172/jci.insight.121971

Human BIN1 isoforms grow, maintain, and regenerate excitation–contraction couplons in adult rat and human stem cell-derived cardiomyocytes

Author(s): Jia Guo, Qinghai Tian, Monika Barth, Wenying Xian, Sandra Ruppenthal, Hans-Joachim Schaefers, Zhifen Chen, Alessandra Moretti, Karl-Ludwig Laugwitz, Peter Lipp
Published in: Cardiovascular Research, 2021, ISSN 0008-6363
Publisher: Elsevier BV
DOI: 10.1093/cvr/cvab195

Ex vivo Culture and Contractile Force Measurements of Non-human Primate Heart Slices

Author(s): Christine M Poch, Andreas Dendorfer, Karl-Ludwig Laugwitz, Alessandra Moretti
Published in: Bio-protocol, Issue 13, 2023, Page(s) e4750, ISSN 2331-8325
Publisher: Bio-protocol LLC
DOI: 10.21769/bioprotoc.4750

Deciphering the Role of Wnt and Rho Signaling Pathway in iPSC-Derived ARVC Cardiomyocytes by In Silico Mathematical Modeling

Author(s): Elvira Immacolata Parrotta, Anna Procopio, Stefania Scalise, Claudia Esposito, Giovanni Nicoletta, Gianluca Santamaria, Maria Teresa De Angelis, Tatjana Dorn, Alessandra Moretti, Karl-Ludwig Laugwitz, Francesco Montefusco, Carlo Cosentino, Giovanni Cuda
Published in: International Journal of Molecular Sciences, Issue 22/4, 2021, Page(s) 2004, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22042004

Progressive stretch enhances growth and maturation of 3D stem-cell-derived myocardium

Author(s): Kun Lu, Thomas Seidel, Xiaochun Cao-Ehlker, Tatjana Dorn, Aarif Mohamed Nazeer Batcha, Christine Maria Schneider, Marie Semmler, Tilmann Volk, Alessandra Moretti, Andreas Dendorfer, Roland Tomasi
Published in: Theranostics, 2021, ISSN 1838-7640
Publisher: Ivyspring International Publisher
DOI: 10.7150/thno.54999

Generation of two human iPSC lines, HMGUi003-A and MRIi028-A, carrying pathogenic biallelic variants in the PPCS gene

Author(s): Arcangela Iuso, Fangfang Zhang, Ejona Rusha, Birgit Campbell, Tatjana Dorn, Enrica Zanuttigh, Dorothea Haas, Yair Anikster, Gabriele Lederer, Anna Pertek, Polyxeni Nteli, Karl-Ludwig Laugwitz, Alessandra Moretti
Published in: Stem cell research, Issue 61, 2022, Page(s) 102773, ISSN 1876-7753
Publisher: Elsevier
DOI: 10.1016/j.scr.2022.102773

High-throughput optical action potential recordings in hiPSC-derived cardiomyocytes with a genetically encoded voltage indicator in the AAVS1 locus

Author(s): Fangfang Zhang, Anna B. Meier, Christine M. Poch, Qinghai Tian, Stefan Engelhardt, Daniel Sinnecker, Peter Lipp, Karl-Ludwig Laugwitz, Alessandra Moretti, Tatjana Dorn
Published in: Frontiers in Cell and Developmental Biology, 2022, ISSN 2296-634X
Publisher: Frontiers Media S.A.
DOI: 10.3389/fcell.2022.1038867

Functional abnormalities in induced Pluripotent Stem Cell-derived cardiomyocytes generated from titin-mutated patients with dilated cardiomyopathy

Author(s): Revital Schick, Lucy N. Mekies, Yuval Shemer, Binyamin Eisen, Tova Hallas, Ronen Ben Jehuda, Meital Ben-Ari, Agnes Szantai, Lubna Willi, Rita Shulman, Michael Gramlich, Luna Simona Pane, Ilaria My, Dov Freimark, Marta Murgia, Gianluca Santamaria, Mihaela Gherghiceanu, Michael Arad, Alessandra Moretti, Ofer Binah
Published in: PLOS ONE, Issue 13/10, 2018, Page(s) e0205719, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0205719

Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus

Author(s): Fangfang Zhang, Anna B. Meier, Daniel Sinnecker, Stefan Engelhardt, Peter Lipp, Karl-Ludwig Laugwitz, Tatjana Dorn, Alessandra Moretti
Published in: Stem Cell Research, Issue 61, 2022, Page(s) 102785, ISSN 1876-7753
Publisher: Elsevier
DOI: 10.1016/j.scr.2022.102785

Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease

Author(s): Meier AB, Zawada D, De Angelis MT, Martens LD, Santamaria G, Zengerle S, Nowak-Imialek M, Kornherr J, Zhang F, Tian Q, Wolf CM, Kupatt C, Sahara M, Lipp P, Theis FJ, Gagneur J, Goedel A, Laugwitz KL, Dorn T, Moretti A
Published in: Nature Biotechnology, 2023, ISSN 1087-0156
Publisher: Nature Publishing Group
DOI: 10.1038/s41587-023-01718-7

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

Author(s): Alexander Goedel, Dorota M. Zawada, Fangfang Zhang, Zhifen Chen, Alessandra Moretti, Daniel Sinnecker
Published in: Journal of Visualized Experiments, Issue 139, 2018, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/58134

Aberrant Deactivation-Induced Gain of Function in TRPM4 Mutant Is Associated with Human Cardiac Conduction Block

Author(s): Wenying Xian, Xin Hui, Qinghai Tian, Hongmei Wang, Alessandra Moretti, Karl-Ludwig Laugwitz, Veit Flockerzi, Sandra Ruppenthal, Peter Lipp
Published in: Cell Reports, Issue 24/3, 2018, Page(s) 724-731, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2018.06.034

Interplay of cell–cell contacts and RhoA/ MRTF ‐A signaling regulates cardiomyocyte identity

Author(s): Tatjana Dorn, Jessica Kornherr, Elvira I Parrotta, Dorota Zawada, Harold Ayetey, Gianluca Santamaria, Laura Iop, Elisa Mastantuono, Daniel Sinnecker, Alexander Goedel, Ralf J Dirschinger, Ilaria My, Svenja Laue, Tarik Bozoglu, Christian Baarlink, Tilman Ziegler, Elisabeth Graf, Rabea Hinkel, Giovanni Cuda, Stefan Kääb, Andrew A Grace, Robert Grosse, Christian Kupatt, Thomas Meitinger, Austin G S
Published in: The EMBO Journal, Issue 37/12, 2018, ISSN 0261-4189
Publisher: Nature Publishing Group
DOI: 10.15252/embj.201798133

The Wnt inhibitor Dkk1 is required for maintaining the normal cardiac differentiation program in Xenopus laevis

Author(s): Yanchun Guo, Tatjana Dorn, Susanne J. Kühl, Alexander Linnemann, Melanie Rothe, Astrid S. Pfister, Seppo Vainio, Karl-Ludwig Laugwitz, Alessandra Moretti, Michael Kühl
Published in: Developmental Biology, Issue 449/1, 2019, Page(s) 1-13, ISSN 0012-1606
Publisher: Academic Press
DOI: 10.1016/j.ydbio.2019.02.009

Somatic gene editing ameliorates skeletal and cardiac muscle failure in pig and human models of Duchenne muscular dystrophy

Author(s): A. Moretti, L. Fonteyne, F. Giesert, P. Hoppmann, A. B. Meier, T. Bozoglu, A. Baehr, C. M. Schneider, D. Sinnecker, K. Klett, T. Fröhlich, F. Abdel Rahman, T. Haufe, S. Sun, V. Jurisch, B. Kessler, R. Hinkel, R. Dirschinger, E. Martens, C. Jilek, A. Graf, S. Krebs, G. Santamaria, M. Kurome, V. Zakhartchenko, B. Campbell, K. Voelse, A. Wolf, T. Ziegler, S. Reichert, S. Lee, F. Flenkenthaler, T. Do
Published in: Nature Medicine, Issue 26/2, 2020, Page(s) 207-214, ISSN 1078-8956
Publisher: Nature Publishing Group
DOI: 10.1038/s41591-019-0738-2

DGK and DZHK position paper on genome editing: basic science applications and future perspective

Author(s): Ralf P. Brandes, Anne Dueck, Stefan Engelhardt, Manuel Kaulich, Christian Kupatt, Maria Teresa De Angelis, Matthias S. Leisegang, Ferdinand le Noble, Alessandra Moretti, Oliver J. Müller, Boris V. Skryabin, Thomas Thum, Wolfgang Wurst
Published in: Basic Research in Cardiology, Issue 116/1, 2021, ISSN 0300-8428
Publisher: Dr. Dietrich Steinkopff Verlag
DOI: 10.1007/s00395-020-00839-3

Generation of heterozygous (MRli003-A-3) and homozygous (MRli003-A-4) TRPM4 knockout human iPSC lines

Author(s): Fangfang Zhang, Anna B. Meier, Peter Lipp, Karl-Ludwig Laugwitz, Tatjana Dorn, Alessandra Moretti
Published in: Stem Cell Research, Issue 60, 2022, Page(s) 102731, ISSN 1876-7753
Publisher: Elsevier
DOI: 10.1016/j.scr.2022.102731

Sequential Defects in Cardiac Lineage Commitment and Maturation Cause Hypoplastic Left Heart Syndrome

Author(s): Markus Krane, Martina Dreßen, Gianluca Santamaria, Ilaria My, Christine M. Schneider, Tatjana Dorn, Svenja Laue, Elisa Mastantuono, Riccardo Berutti, Hilansi Rawat, Ralf Gilsbach, Pedro Schneider, Harald Lahm, Sascha Schwarz, Stefanie A. Doppler, Sharon Paige, Nazan Puluca, Sophia Doll, Irina Neb, Thomas Brade, Zhong Zhang, Claudia Abou-Ajram, Bernd Northoff, Lesca M. Holdt, Stefanie Sudhop, Mako
Published in: Circulation, Issue 144/17, 2021, Page(s) 1409-1428, ISSN 0009-7322
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1161/circulationaha.121.056198

Endothelial Retargeting of AAV9 In Vivo

Author(s): Tarik Bozoglu, Seungmin Lee, Tilman Ziegler, Victoria Jurisch, Sanne Maas, Andrea Baehr, Rabea Hinkel, Amelie Hoenig, Anjana Hariharan, Christina Inyeop Kim, Simon Decker, Haider Sami, Tobias Koppara, Ruppert Oellinger, Oliver J. Müller, Derk Frank, Remco Megens, Peter Nelson, Christian Weber, Angelika Schnieke, Markus Sperandio, Gianluca Santamaria, Roland Rad, Alessandra Moretti, Karl‐Ludwig
Published in: Advanced science, Issue 9(7), 2022, Page(s) 2103867, ISSN 2198-3844
Publisher: WILEY-VCH
DOI: 10.1002/advs.202103867

Transcriptome Analysis of Reticulated Platelets Reveals a Prothrombotic Profile

Author(s): Dario Bongiovanni, Gianluca Santamaria, Melissa Klug, Donato Santovito, Arianna Felicetta, Michael Hristov, Moritz von Scheidt, Maria Aslani, Javier Cibella, Christian Weber, Alessandra Moretti, Karl-Ludwig Laugwitz, Clelia Peano, Isabell Bernlochner
Published in: Thrombosis and Haemostasis, Issue 119/11, 2019, Page(s) 1795-1806, ISSN 0340-6245
Publisher: Schattauer
DOI: 10.1055/s-0039-1695009

Genome editing for Duchenne muscular dystrophy: a glimpse of the future?

Author(s): Christian Kupatt, Alina Windisch, Alessandra Moretti, Eckhard Wolf, Wolfgang Wurst, Maggie C. Walter
Published in: Gene Therapy, 2021, ISSN 0969-7128
Publisher: Nature Publishing Group
DOI: 10.1038/s41434-021-00222-4

Cell cycle defects underlie childhood-onset cardiomyopathy associated with Noonan syndrome

Author(s): Anna B Meier, Sarala Raj Murthi, Hilansi Rawat, Christopher N Toepfer, Gianluca Santamaria, Manuel Schmid, Elisa Mastantuono, Thomas Schwarzmayr, Riccardo Berutti, Julie Cleuziou, Peter Ewert, Agnes Görlach, Karin Klingel, Karl-Ludwig Laugwitz, Christine E Seidman, Jonathan G Seidman, Alessandra Moretti, Cordula M Wolf
Published in: iScience, Issue 25(1), 2021, Page(s) 103596, ISSN 2589-0042
Publisher: Cell-Press
DOI: 10.1016/j.isci.2021.103596