Quantification of the intestinal load of a targeted set of resistance genes to Monitor Antibiotic Resistance in paediatric transplant patients

Projektinformationen

qMAR

ID Finanzhilfevereinbarung: 796084

DOI 10.3030/796084

Abgeschlossenes Projekt

EK-Unterschriftsdatum 23 April 2018

Startdatum 1 September 2018

Enddatum 23 November 2020

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten € 158 121,60

EU-Beitrag € 158 121,60

Koordiniert durch

FUNDACION PARA LA INVESTIGACION BIOMEDICA DEL HOSPITAL UNIVERSIATRIO LA PAZ
Spain

Ziel

The gut microbiome is involved in numerous processes that include protection against colonization and infection by pathogenic organisms. The extensive use of antibiotics could result in dysbiosis with detrimental effects on the health of the individual, in addition to increasing the risk of driving the emergence of multi-drug resistant organisms. This is especially concerning among paediatric transplant patient, a group of patients with special needs that are heavily reliant on antibiotics after surgery. It has been shown that determining the load of resistance genes among the gut microbial organisms could be a predictive factor for the development of pathogenic infections. Along these lines, qMAR’s main objective is to evaluate the use of qPCR for monitoring biomarkers, in terms of the intestinal load of selected antibiotic resistance genes in the resistome over time, as tools that could be used in PMed approaches for paediatric transplant patients.
In order to achieve this, faecal samples and medical records will be collected from each paediatric transplant patients over a period of 12 months. Antibiotic susceptibility testing will be performed using VITEK-2. qPCR will be performed directly on the samples in order to determine the load of epidemiologically relevant resistant genes over time. Multi-locus sequence typing will be performed for determining the clonality of the isolates. Statistical analyses will also be performed in order to determine associations between clinical interventions, the load of resistance genes, and clinical outcomes. The main expected result is determining a threshold of resistance genes after which adverse clinical outcomes would be expected, and determining how clinical interventions are affecting the load of resistance genes and the microbiome.

Wissenschaftliches Gebiet

• medical and health sciences › basic medicine › pharmacology and pharmacy › pharmaceutical drugs › antibiotics
• medical and health sciences › clinical medicine › paediatrics
• medical and health sciences › basic medicine › pharmacology and pharmacy › drug resistance › multidrug resistance
• natural sciences › biological sciences › microbiology
• medical and health sciences › basic medicine › pharmacology and pharmacy › drug resistance › antibiotic resistance

Programm/Programme

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thema/Themen

• MSCA-IF-2017 - Individual Fellowships

Aufforderung zur Vorschlagseinreichung

H2020-MSCA-IF-2017

Finanzierungsplan

MSCA-IF-EF-SE - Society and Enterprise panel

Koordinator

FUNDACION PARA LA INVESTIGACION BIOMEDICA DEL HOSPITAL UNIVERSIATRIO LA PAZ

• Netto-EU-Beitrag: € 158 121,60
• Adresse: PASEO DE LA CASTELLANA 261 ; 28046 Madrid ; Spanien
• Region: Comunidad de Madrid › Comunidad de Madrid › Madrid
• Aktivitätstyp: Other
• Gesamtkosten: € 158 121,60