Descripción del proyecto
Información sobre el papel del nicho de las células madre del cáncer
Los citoblastos histoespecíficos son células indiferenciadas que tienen la capacidad de autorrenovarse y generar varios tipos de células dentro del tejido. El mantenimiento y la función de las células madre dependen del nicho de las células madre, un lugar especializado dentro de los tejidos. El equipo del proyecto IntratumoralNiche, financiado con fondos europeos, trabaja con la hipótesis de que en los tumores existe un nicho parecido que favorece las células madre del cáncer, el crecimiento del tumor y la formación de metástasis. Los investigadores pretenden examinar los mecanismos de señalización del nicho intratumoral utilizando cultivos de organoides derivados de pacientes y modelos animales de cáncer colorrectal. Los resultados desvelarán el papel de los diferentes componentes del nicho en la progresión del tumor, lo que permitiría identificar nuevas dianas terapéuticas.
Objetivo
Purpose: Cells in a tumor are highly heterogeneous. The role and consequence of having multiple cell types within a cancer is mostly centered towards the function of cancer stem cells (CSCs) since they are the driving forces of tumor growth. However, the exact signaling cues that support CSC function remain to be understood. For instance, what are the roles of immediate descendant tumor cells in relation to CSC support? Do colorectal tumors make their own niche?
Preliminary data: To study communication between different cell types (heterocellular signaling) in human colorectal cancers (CRCs), my lab developed movieSTAR technology to mark CSCs in patient-derived CRC organoids (PDOs) for high-resolution live imaging of their dynamics and behavior. Although niche factor dependency decreases along the adenoma-carcinoma transition, we identified a strong interdependency between CSCs and other tumor cells in colorectal PDOs of malignant nature.
Hypothesis: We hypothesize a continuous existence of an intratumoral stem cell niche that remains essential for tumor growth and metastasis formation. Which types of heterocellular signaling support CSC function, especially at malignant stages, is unknown.
Approach: This project aims to define heterocellular signaling between CSCs and intratumoral niche cells. Therefore, I) we will combine our expertise in human organoid technology for in-depth characterization of the nature of heterocellular communication within the intratumoral niche, II) high-resolution live imaging of PDOs to interrogate heterogeneity of signaling activities at cellular resolution and in real-time, as well as III) in vivo mouse models for validation and further studies of essential intratumoral signaling pathways.
Innovation: Our integrative use of novel approaches will provide comprehensive insight into intratumoral niche function during tumorigenesis, establishing novel technologies for future cancer research and new concepts to improve cancer therapy.
Ámbito científico
Programa(s)
Régimen de financiación
ERC-STG - Starting GrantInstitución de acogida
3584 CX Utrecht
Países Bajos