Descrizione del progetto
Comprendere il ruolo della nicchia delle cellule staminali tumorali
Le cellule staminali specifiche del tessuto sono cellule indifferenziate dotate della capacità di auto-rinnovarsi e generare più tipi di cellule all’interno del tessuto. Il mantenimento e la funzione delle cellule staminali dipendono dalla nicchia delle cellule staminali, una posizione specializzata all’interno dei tessuti. Il progetto IntratumoralNiche, finanziato dall’UE, lavora sull’ipotesi che una nicchia simile esista nei tumori e sostenga le cellule staminali tumorali, la crescita del tumore e la formazione di metastasi. I ricercatori intendono analizzare i segnali della nicchia intratumorale utilizzando colture di organoidi derivate da pazienti e modelli animali di tumore colorettale. I risultati sveleranno il ruolo dei diversi componenti della nicchia nella progressione del tumore, identificando potenzialmente nuovi bersagli terapeutici.
Obiettivo
Purpose: Cells in a tumor are highly heterogeneous. The role and consequence of having multiple cell types within a cancer is mostly centered towards the function of cancer stem cells (CSCs) since they are the driving forces of tumor growth. However, the exact signaling cues that support CSC function remain to be understood. For instance, what are the roles of immediate descendant tumor cells in relation to CSC support? Do colorectal tumors make their own niche?
Preliminary data: To study communication between different cell types (heterocellular signaling) in human colorectal cancers (CRCs), my lab developed movieSTAR technology to mark CSCs in patient-derived CRC organoids (PDOs) for high-resolution live imaging of their dynamics and behavior. Although niche factor dependency decreases along the adenoma-carcinoma transition, we identified a strong interdependency between CSCs and other tumor cells in colorectal PDOs of malignant nature.
Hypothesis: We hypothesize a continuous existence of an intratumoral stem cell niche that remains essential for tumor growth and metastasis formation. Which types of heterocellular signaling support CSC function, especially at malignant stages, is unknown.
Approach: This project aims to define heterocellular signaling between CSCs and intratumoral niche cells. Therefore, I) we will combine our expertise in human organoid technology for in-depth characterization of the nature of heterocellular communication within the intratumoral niche, II) high-resolution live imaging of PDOs to interrogate heterogeneity of signaling activities at cellular resolution and in real-time, as well as III) in vivo mouse models for validation and further studies of essential intratumoral signaling pathways.
Innovation: Our integrative use of novel approaches will provide comprehensive insight into intratumoral niche function during tumorigenesis, establishing novel technologies for future cancer research and new concepts to improve cancer therapy.
Campo scientifico
Programma(i)
Argomento(i)
Meccanismo di finanziamento
ERC-STG - Starting GrantIstituzione ospitante
3584 CX Utrecht
Paesi Bassi