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Strengthening the Research Area of Delivery of Nucleic acid mimics (NAMS) into bacteria to fight the antibiotic crisis

Deliverables

Annual report on high impact publications

At the end of each year an annual report will be delivered regarding the high impact publications from the project (12, 24 and 36th month).

Website and social media sites

Project website and social media will be ready to maximize project visibility.

Publications

Intracellular delivery of oligonucleotides in Helicobacter pylori by fusogenic liposomes in the presence of gastric mucus

Author(s): Rita S. Santos, George R. Dakwar, Elisa Zagato, Toon Brans, Céu Figueiredo, Koen Raemdonck, Nuno F. Azevedo, Stefaan C. De Smedt, Kevin Braeckmans
Published in: Biomaterials, Issue 138, 2017, Page(s) 1-12, ISSN 0142-9612
DOI: 10.1016/j.biomaterials.2017.05.029

An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium

Author(s): Andreia S. Azevedo, Carina Almeida, Luciana C. Gomes, Carla Ferreira, Filipe J. Mergulhão, Luís F. Melo, Nuno F. Azevedo
Published in: Biochemical Engineering Journal, Issue 118, 2017, Page(s) 64-69, ISSN 1369-703X
DOI: 10.1016/j.bej.2016.11.013

Novel strategy to detect and locate periodontal pathogens: The PNA-FISH technique

Author(s): Luzia Mendes, Rui Rocha, Andreia Sofia Azevedo, Catarina Ferreira, Mariana Henriques, Miguel Gonçalves Pinto, Nuno Filipe Azevedo
Published in: Microbiological Research, Issue 192, 2016, Page(s) 185-191, ISSN 0944-5013
DOI: 10.1016/j.micres.2016.07.002

Fluorescence In Vivo Hybridization (FIVH) for Detection of Helicobacter pylori Infection in a C57BL/6 Mouse Model

Author(s): Sílvia Fontenete, Marina Leite, Davie Cappoen, Rita Santos, Chris Van Ginneken, Céu Figueiredo, Jesper Wengel, Paul Cos, Nuno Filipe Azevedo
Published in: PLOS ONE, Issue 11/2, 2016, Page(s) e0148353, ISSN 1932-6203
DOI: 10.1371/journal.pone.0148353

Application of locked nucleic acid-based probes in fluorescence in situ hybridization

Author(s): Sílvia Fontenete, Daniel Carvalho, Nuno Guimarães, Pedro Madureira, Céu Figueiredo, Jesper Wengel, Nuno Filipe Azevedo
Published in: Applied Microbiology and Biotechnology, Issue 100/13, 2016, Page(s) 5897-5906, ISSN 0175-7598
DOI: 10.1007/s00253-016-7429-4

Optimization of peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria: The effect of pH, dextran sulfate and probe concentration

Author(s): Rui Rocha, Rita S. Santos, Pedro Madureira, Carina Almeida, Nuno F. Azevedo
Published in: Journal of Biotechnology, Issue 226, 2016, Page(s) 1-7, ISSN 0168-1656
DOI: 10.1016/j.jbiotec.2016.03.047

Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori

Author(s): Rita S Santos, George R Dakwar, Ranhua Xiong, Katrien Forier, Katrien Remaut, Stephan Stremersch, Nuno Guimarães, Sílvia Fontenete, Jesper Wengel, Marina Leite, Céu Figueiredo, Stefaan C De Smedt, Kevin Braeckmans, Nuno F Azevedo
Published in: Molecular Therapy - Nucleic Acids, Issue 4, 2015, Page(s) e269, ISSN 2162-2531
DOI: 10.1038/mtna.2015.46

Towards Fluorescence In Vivo Hybridization (FIVH) Detection of H. pylori in Gastric Mucosa Using Advanced LNA Probes

Author(s): Sílvia Fontenete, Marina Leite, Nuno Guimarães, Pedro Madureira, Rui Manuel Ferreira, Céu Figueiredo, Jesper Wengel, Nuno Filipe Azevedo
Published in: PLOS ONE, Issue 10/4, 2015, Page(s) e0125494, ISSN 1932-6203
DOI: 10.1371/journal.pone.0125494

Detection and discrimination of biofilm populations using locked nucleic acid/2′-O-methyl-RNA fluorescence in situ hybridization (LNA/2′OMe-FISH)

Author(s): Andreia S. Azevedo, Carina Almeida, Bruno Pereira, Pedro Madureira, Jesper Wengel, Nuno F. Azevedo
Published in: Biochemical Engineering Journal, Issue 104, 2015, Page(s) 64-73, ISSN 1369-703X
DOI: 10.1016/j.bej.2015.04.024

Mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids

Author(s): Fontenete Silvia, Barros Joana, Madureira Pedro, Figueiredo Céu, Wengel Jesper, Azevedo Nuno Filipe
Published in: Applied Microbiology and Biotechnology, Issue 99/9, 2015, Page(s) 3961-3969, ISSN 0175-7598
DOI: 10.1007/s00253-015-6389-4

Alpha-l-Locked Nucleic Acid-Modified Antisense Oligonucleotides Induce Efficient Splice Modulation In Vitro

Author(s): Prithi Raguraman, Tao Wang, Lixia Ma, Per Trolle Jørgensen, Jesper Wengel, Rakesh N. Veedu
Published in: International Journal of Molecular Sciences, Issue 21/7, 2020, Page(s) 2434, ISSN 1422-0067
DOI: 10.3390/ijms21072434

Fluorinated Molecules and Nanotechnology: Future ‘Avengers’ against the Alzheimer’s Disease?

Author(s): Meghna Dabur, Joana A. Loureiro, Maria Carmo Pereira
Published in: International Journal of Molecular Sciences, Issue 21/8, 2020, Page(s) 2989, ISSN 1422-0067
DOI: 10.3390/ijms21082989

Molecular interactions between Vitamin B12 and membrane models: A biophysical study for new insights into the bioavailability of Vitamin

Author(s): Maria João Ramalho, Stéphanie Andrade, Manuel A.N. Coelho, Joana A. Loureiro, Maria Carmo Pereira
Published in: Colloids and Surfaces B: Biointerfaces, Issue 194, 2020, Page(s) 111187, ISSN 0927-7765
DOI: 10.1016/j.colsurfb.2020.111187

Novel assemblies based on oligonucleotides containing intercalating nucleic acid monomers


Published in: ISSN 1525-7770
DOI: 10.1080/15257770.2019.1683188

Nanomaterials and molecular transporters to overcome the bacterial envelope barrier: Towards advanced delivery of antibiotics

Author(s): Rita S. Santos, Céu Figueiredo, Nuno F. Azevedo, Kevin Braeckmans, Stefaan C. De Smedt
Published in: Advanced Drug Delivery Reviews, Issue 136-137, 2018, Page(s) 28-48, ISSN 0169-409X
DOI: 10.1016/j.addr.2017.12.010

Effect of lipid concentration on the delivery efficiency of liposome-encapsulated nucleic acid mimics (LipoNAMs) on Escherichia coli.

Author(s): Moreira L, Pereira S, Guimarães N, Santos RS, Pereira C, Azevedo NF.
Published in: 29th ECCMID, 2019

Delivery of nucleic acid mimics (NAMs) by liposomes into Gram-negative and Gram-positive bacteria.

Author(s): Pereira S, Santos RS, Moreira L, Guimarães N, Azevedo NF, Braeckmans K, De Smedt SC.
Published in: 29th ECCMID, 2019

Delivery of nucleic acid mimics (NAMs) in bacteria towards fighting antimicrobial resistance.

Author(s): Santos R, Pereira S, De Smedt S, Braeckmans K, Azevedo NF.
Published in: BioNano-20, 2020

DelNAM – fighting the crisis of bacterial resistance to antibiotics.

Author(s): Santos RS, Wengel J, Braeckmans K, De Smedt SC, Azevedo NF.
Published in: NanoMed Europe 2019, 2019

Liposome-encapsulated nucleic acid mimics for the delivery of oligonucleotides into bacteria.




Delivery of nucleic acid mimics (NAMs) in bacteria mediated by lipoplexes.

Author(s): Pereira S, Santos RS, Moreira L, Zhang H, Guimarães N, Remaut K, Azevedo NF, Braeckmans K, de Smedt SC.
Published in: XIIIth Spanish-Portuguese Conference on Controlled Drug Delivery, 2020

Delivery of nucleic acid mimics to target Helicobacter pylori infections – the effect of biological barriers.

Author(s): Santos RS, Dakwar GR, Zagato E, Brans T, Figueiredo C, Azevedo NF, De Smedt SC, Braeckmans K.
Published in: XIIIth Spanish-Portuguese Conference on Controlled Drug Delivery, 2020

Interaction of cationic liposomes with Gram-negative and Gram-positive bacteria.

Author(s): Moreira L, Pereira S, Guimarães N, Santos RS, Loureiro J, Pereira C, Azevedo NF.
Published in: XIIIth Spanish-Portuguese Conference on Controlled Drug Delivery, 2020

Immunoliposome for the delivery of therapeutic oligonucleotides into bacteria.

Author(s): Moreira L, Pereira S, Guimarães N, Santos RS, Loureiro J, Pereira C, Azevedo NF.
Published in: ASM Microbe 2020, 2020

Delivery of nucleic acid mimics (NAMs) in bacteria mediated by lipoplexes.

Author(s): Pereira S, Santos RS, Moreira L, Zhang H, Guimarães N, Remaut K, Azevedo NF, Braeckmans K, De Smedt SC.
Published in: ASM Microbe 2020, 2020