Biomechanics in health and disease: advanced physical tools for innovative early diagnosis

Información del proyecto

Phys2BioMed

Identificador del acuerdo de subvención: 812772

DOI

10.3030/812772

Proyecto cerrado

Fecha de la firma de la CE 20 Agosto 2018

Fecha de inicio 1 Enero 2019

Fecha de finalización 31 Diciembre 2022

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 3 911 782,68

Aportación de la UE

€ 3 911 782,68

Coordinado por

UNIVERSITA DEGLI STUDI DI MILANO
Italy

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Other (31)

Viscoelastic fingerprint of cells and tissues

Determine the typical viscoelastic fingerprint of cells and tissues.

Viscoelasticity of cells and tissues by nano-indenter

Characterisation of the viscoelastic properties of cells and tissues using a nano-indenter.

Workshops

Organisation of Workshops, planned for Months 6,18,30,42.

Organisation of secondments

Organisation of secondments of ESRs (from Month 2 to Month 48).

Scientific publications

Writing of scientific publications and reviews and open access publications (the process will be monitored during the whole project, and the final check will be done at the end).

Accuracy of mechanical tests

Determine the accuracy of mechanical tests, and identify the best measurement conditions.

Project meetings

Project meetings are planned at months 2,6,12,18,24,30,36,42,48.

Online resources

Management of online resources: wiki, webpage, social networks.

Contribution of ESRs to workshops, schools, and international conferences

Contribution of ESRs to workshops, schools, and international conferences. Planned roughly 6-monthly, starting from Month 6.

Recruitment of ESRs and PhD registration

Recruitment of ESRs and registration into the PhD programme.

Supervisory Board

Definition of the Supervisory Board of the network.

Identification of mechanical markers of diseases

Identification of specific mechanical markers of selected diseases.

Identification of links between biomechanics and disease

Identification of general links between biomechanics and disease.

Awarding of PhD title to ESRs

Awarding of PhD title to ESRs.

Schools

Organisation of Schools, planned at Months 12, 24, 36.

Database of mechanical fingerprints

Populate the database of mechanical fingerprints of disease with data collected for various relevant cases.

Mechanical model of cells and tissues

Develop mechanical models of cells and tissues, to interpret experimental data.

Definition of secondments plan

All secondments plans must be prepared within this date.

Viscoelasticity of cells by microfluidics

Development of characterisation strategies of viscoelasticity of cells by microfluidic tools (beyond AFM).

Optimised correlative approaches

Development of optimised correlative approaches for the study of cell and tissue mechanics.

Standardised procedures for sample shipping

Development of standardised procedures for cells and tissues transport and shipping.

Consolidating middle-throughput mechanical testing

Consolidate a methodology to run mechanical tests on multiple samples at the middle-throughput level.

Final symposium

Organisation of the final project symposium.

Data processing open source software

Develop data processing open source software and applets.

Nano-biomechanics for schoolchildren

Nano-biomechanics communicated to schoolchildren (roughly annual).

MOOC

Organisation of a Massive Open Online Course (MOOC).

Determination of mechanical fingerprints of clinical samples

Determination of the major mechanical fingerprints of clinical samples, in health and disease.

Universal file format

Definition of a common universal file format for saving and sharing mechanical data in different laboratories.

Social networking

Social networking: creation and maintenance of Twitter, LinkedIn, ORCID and ResearchID accounts.

Identification of Characteristics of mechanical dynamics

Identification of the characteristics of the dynamical mechanical properties of cells and tissues.

Wiki accessible to the scientific community

Wiki made accessible to the scientific community.

Documents, reports (6)

Progress Report

Preparation of the Progress Report.

Standardised procedure for preparation of tissues for mechanical testing

Definition of standardised procedures for the preparation of tissues for testing their mechanical properties.

Standardised cell culture conditions for mechanical testing

Definition of standardised cell culture conditions for mechanical testing.

Standardised procedure for tissue mechanics

Definition of standardised procedures for the characterisation of tissue mechanics.

Standardised procedure for immobilisation of tissues for mechanical testing

Definition of standardised procedures for the immobilisation of tissues for testing their mechanical properties.

Standardised procedure for cell mechanics

Development of standardised procedures for performing cell mechanics experiments.

Open Research Data Pilot (1)

Open access to raw data

Deposition of raw data in open access repository according to ORDP rules

Websites, patent fillings, videos etc. (1)

Website

Project website running.

Publicaciones

Peer reviewed articles (34)

Novel Decellularization Method for Tissue Slices

Autores: Narciso M, Ulldemolins A, Júnior C, Otero J, Navajas D, Farré R, Gavara N, Almendros I
Publicado en: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Editor: Frontiers Media S.A.
DOI: 10.3389/fbioe.2022.832178

Image-Based Method to Quantify Decellularization of Tissue Sections.

Autores: Maria Narciso; Jorge Otero; Daniel Navajas; Ramon Farré; Isaac Almendros; Núria Gavara
Publicado en: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22168399

Traction force microscopy – Measuring the forces exerted by cells

Autores: Malgorzata Lekka, Kajangi Gnanachandran, Andrzej Kubiak, Tomasz Zielinski, Joanna Zemla
Publicado en: Micron, Edición 150, 2021, Página(s) 103138, ISSN 0968-4328
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.micron.2021.103138

Decellularized extracellular matrix as scaffold for cancer organoid cultures of colorectal peritoneal metastases

Autores: Luca Varinelli, Marcello Guaglio, Silvia Brich, Susanna Zanutto, Antonino Belfiore, Federica Zanardi, Fabio Iannelli, Amanda Oldani, Elisa Costa, Matteo Chighizola, Ewelina Lorenc, Simone P Minardi, Stefano Fortuzzi, Martina Filugelli, Giovanna Garzone, Federica Pisati, Manuela Vecchi, Giancarlo Pruneri, Shigeki Kusamura, Dario Baratti, Laura Cattaneo, Dario Parazzoli, Alessandro Podestà, Massimo
Publicado en: Journal of Molecular Cell Biology, 2022, ISSN 1759-4685
Editor: Oxford University Press
DOI: 10.1093/jmcb/mjac064

Mutated lamin A modulates stiffness in muscle cells

Autores: Maria Chatzifrangkeskou, Delf Kah, Janina R. Lange, Wolfgang H. Goldmann, Antoine Muchir
Publicado en: Biochemical and Biophysical Research Communications, Edición 529/3, 2020, Página(s) 861-867, ISSN 0006-291X
Editor: Academic Press
DOI: 10.1016/j.bbrc.2020.05.102

Silk-Reinforced Collagen Hydrogels with Raised Multiscale Stiffness for Mesenchymal Cells 3D Culture

Autores: Sanz-Fraile, H., Amoros, S., Mendizabal, I., Galvez-Monton, C., Prat-Vidal, C., Bayes-Genis, A., Navajas, D., Farre, R., & Otero, J.
Publicado en: Tissue Engineering Part A, 2020, ISSN 1937-335X
Editor: Mary Ann Liebert Inc.
DOI: 10.1089/ten.tea.2019.0199

Applicability of atomic force microscopy to determine cancer-related changes in cells

Autores: Malgorzata Lekka
Publicado en: Phil. Trans. R. Soc. A, 2022, ISSN 1471-2962
Editor: The Royal Society publishing
DOI: 10.1098/rsta.2021.0346

The glycocalyx affects the mechanotransductive perception of the topographical microenvironment

Autores: Matteo Chighizola; Tania Dini; Stefania Marcotti; Mirko D’Urso; Claudio Piazzoni; Francesca Borghi; Anita Previdi; Laura Ceriani; Claudia Folliero; Brian Stramer; Cristina Lenardi; Paolo Milani; Alessandro Podestà; Carsten Schulte
Publicado en: Journal of Nanobiotechnology, 2022, Página(s) 418, ISSN 1477-3155
Editor: BioMed Central
DOI: 10.1186/s12951-022-01585-5

Universal Model for the Log-Normal Distribution of Elasticity in Polymeric Gels and Its Relevance to Mechanical Signature of Biological Tissues.

Autores: Arnaud Millet
Publicado en: Biology (Basel), Edición 10(1), 2021, Página(s) 64, ISSN 2079-7737
Editor: Biology
DOI: 10.3390/biology10010064

Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells

Autores: Falcones B, Sanz-Fraile H, Marhuenda E, Mendizábal I, Cabrera-Aguilera I, Malandain N, Uriarte JJ, Almendros I, Navajas D, Weiss DJ, Farré R, Otero J.
Publicado en: Polymers, 2021, ISSN 2073-4360
Editor: MDPI AG
DOI: 10.3390/polym13142350

Nano-structural stiness measure for soft biomaterials of heterogeneous elasticity

Autores: Shu-wen W. Chen, Jean-Marie Teulon, Harinderbir Kaur, Christian Godon and Jean-Luc Pellequer
Publicado en: Nanoscale Horizons, Edición 20556764, 2022, ISSN 2055-6764
Editor: Royal Society of Chemistry
DOI: 10.1039/d2nh00390b

A Fast and Efficient Decellularization Method for Tissue Slices

Autores: Narciso M, Ulldemolins A, Júnior C, Otero J, Navajas D, Farré R, Gavara N, Almendros I
Publicado en: Bio-protocol Journal, 2022, ISSN 2331-8325
Editor: Sunnyvale CA: Bio-protocol LLC
DOI: 10.21769/bioprotoc.4550

Viscoelastic properties of suspended cells measured with shear flow deformation cytometry

Autores: Richard Gerum; Elham Mirzahossein; Mar Eroles; Jennifer Elsterer; Astrid Mainka; Andreas Bauer; Selina Sonntag; Alexander Winterl; Johannes Bartl; Lena Fischer; Shada Abuhattum; Ruchi Goswami; Salvatore Girardo; Jochen Guck; Stefan Schrüfer; Nadine Ströhlein; Mojtaba Nosratlo; Harald Herrmann; Dorothea Schultheis; Felix Rico; Sebastian Müller; Stephan Gekle; Ben Fabry
Publicado en: eLife, 2022, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.78823

Discriminating bladder cancer cells through rheological mechanomarkers at cell and spheroid levels

Autores: Kajangi Gnanachandran, Sylwia Kędracka-Krok, Joanna Pabijan, Małgorzata Lekka
Publicado en: Journal of Biomechanics, Edición 144, 2022, Página(s) 111346, ISSN 0021-9290
Editor: Elsevier BV
DOI: 10.1016/j.jbiomech.2022.111346

Mechanical modeling of lung alveoli: From macroscopic behaviour to cell mechano-sensing at microscopic level

Autores: Daniel Navajas; José Manuel Garcia-Aznar; Gabriel Beltran
Publicado en: Journal of the Mechanical Behavior of Biomedical Materials, 2022, ISSN 1751-6161
Editor: Elsevier BV
DOI: 10.1016/j.jmbbm.2021.105043

Dynamic mechanical analysis of suspended soft bodies via hydraulic force spectroscopy

Autores: Massimiliano Berardi, Kajangi Gnanachandran, Jieke Jiang, Kevin Bielawski, Claas W. Visser, Małgorzata Lekka, B. Imran akca
Publicado en: Soft Matter, 2022, ISSN 1744-683X
Editor: Royal Society of Chemistry
DOI: 10.1039/d2sm01173e

Hypoxia Drives Dihydropyrimidine Dehydrogenase Expression in Macrophages and Confers Chemoresistance in Colorectal Cancer

Autores: Malier M, Gharzeddine K, Laverriere MH, Marsili S, Thomas F, Court M, Decaens T, Roth G, Millet A.
Publicado en: Cancer research, 2021, ISSN 0008-5472
Editor: American Association for Cancer Research
DOI: 10.1158/0008-5472.can-21-1572

Involvement of Mechanical Cues in the Migration of Cajal-Retzius Cells in the Marginal Zone During Neocortical Development

Autores: López-Mengual A, Segura-Feliu M, Sunyer R, Sanz-Fraile H, Otero J, Mesquida-Veny F, Gil V, Hervera A, Ferrer I, Soriano J, Trepat X, Farré R, Navajas D, Del Río JA
Publicado en: Frontiers in Cell and Developmental Biology, 2022, ISSN 2296-634X
Editor: Frontiers Media S.A.
DOI: 10.3389/fcell.2022.886110

Human Septins Organize as Octamer-Based Filaments and Mediate Actin-Membrane Anchoring in Cells.

Autores: Martins C. S., Taveneau C., Castro-Linares G., Baibakov M., Buzhinsky N., Eroles M., Milanović V., Omi S., Pedelacq J.-D., Iv F., Bouillard L., Llewellyn A., Gomes M., Belhabib M., Kuzmić M., Verdier-Pinard P., Lee S., Badache A., Kumar S., Chandre C., Brasselet S., Rico F., Rossier O., Koenderink G. H., Wenger J., Cabantous S., Mavrakis M.
Publicado en: Journal of Cell Biology, 2023, ISSN 0021-9525
Editor: Rockefeller University Press
DOI: 10.1083/jcb.202203016

Baseline Stiffness Modulates the Non-Linear Response to Stretch of the Extracellular Matrix in Pulmonary Fibrosis

Autores: Júnior C, Narciso M, Marhuenda E, Almendros I, Farré R, Navajas D, Otero J, Gavara N
Publicado en: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms222312928

Adhesion force spectroscopy with nanostructured colloidal probes reveals nanotopography-dependent early mechanotransductive interactions at the cell membrane level

Autores: M. Chighizola, A. Previdi, T. Dini, C. Piazzoni, C. Lenardi, P. Milani, C. Schulte, A. Podestà
Publicado en: Nanoscale, Edición 12/27, 2020, Página(s) 14708-14723, ISSN 2040-3364
Editor: Royal Society of Chemistry
DOI: 10.1039/d0nr01991g

Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation System

Autores: Lolo F-N, Walani N, Seemann E, Zalvidea D, Pavón DM, Cojoc G, Zamai M, Viaris de Lesegno C, Martínez de Benito F, Sánchez-Álvarez M, Uriarte JJ, Echarri A, Escolano J-C, Sánchez SA, Caiolfa VR, Navajas D, Trepat X, Guck J, Lamaze C, Roca-Cusachs P, Kessels MM, Qualmann B, Arroyo M, del Pozo MA.
Publicado en: Nat Cell Biol., Edición 25, 2023, Página(s) 120-133, ISSN 1465-7392
Editor: Nature Publishing Group
DOI: 10.1038/s41556-022-01034-3

Multi-step Extracellular Matrix Remodelling and Stiffening in the Development of Idiopathic Pulmonary Fibrosis

Autores: Júnior C, Ulldemolins A, Narciso M, Almendros I, Farre R, Navajas D, Lopez J, Eroles M, Rico F, Gavara N
Publicado en: Int J Mol Sci, Edición 24, 2023, Página(s) 1708, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24021708

Stiffening of DU145 prostate cancer cells driven by actin filaments - microtubule crosstalk conferring resistance to microtubule-targeting drugs.

Autores: Andrzej Kubiak; Matteo Chighizola; Carsten Schulte; Natalia Bryniarska; Julita Wesołowska; Maciej Pudełek; Małgorzata Lasota; Damian Ryszawy; Agnieszka Basta-Kaim; Piotr Laidler; Alessandro Podestà; Małgorzata Lekka
Publicado en: Nanoscale, 2021, ISSN 2040-3372
Editor: Royal Society of Chemistry
DOI: 10.1039/d0nr06464e

Mechanotransduction in neuronal cell development and functioning

Autores: Matteo Chighizola, Tania Dini, Cristina Lenardi, Paolo Milani, Alessandro Podestà, Carsten Schulte
Publicado en: Biophysical Reviews, Edición 11/5, 2019, Página(s) 701-720, ISSN 1867-2450
Editor: Springer Verlag
DOI: 10.1007/s12551-019-00587-2

Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy

Autores: Katerina Karkali, Ignasi Jorba, Daniel Navajas, Enrique Martin-Blanco
Publicado en: STAR protocols, Edición 3, 2022, Página(s) 101901, ISSN 2666-1667
Editor: Cell Press
DOI: 10.1016/j.xpro.2022.101901

Rheological comparison between control and Dupuytren fibroblasts when plated in circular micropatterns using atomic force microscopy

Autores: Sandra Pérez-Domínguez, Elisabeth Werkmeister, Maria Luisa Marini, Vicent Dupres, Sébastien Janel, Frank Lafont, Manfred Radmacher
Publicado en: Frontiers in Physics, Sec. Biophysics, 2022, ISSN 2296-424X
Editor: Frontiers Media
DOI: 10.3389/fphy.2022.1052203

Comparison of rheological properties of healthy versus Dupuytren fibroblasts when treated with a cell contraction inhibitor by Atomic Force Microscope

Autores: Sandra Pérez-Domínguez, Javier López-Alonso, Frank Lafont and Manfred Radmacher
Publicado en: International Journal of Molecular Science, 2023, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24032043

Micro-mechanical fingerprints of the rat bladder change in actinic cystitis and tumor presence

Autores: L. Martinez-Vidal, M. Chighizola, M. Berardi, E. Alchera, I. Locatelli, F. Pederzoli, C. Venegoni, R. Lucianò, P. Milani, K. Bielawski, A. Salonia, A. Podestà, M. Alfano
Publicado en: Communications Biology, 2023, ISSN 2399-3642
Editor: Springer Nature
DOI: 10.1038/s42003-023-04572-0

Atomic force microscopy for cell mechanics and diseases

Autores: Sandra Pérez-Domínguez, Shruti G. Kulkarni, Carmela Rianna, Manfred Radmacher
Publicado en: Neuroforum, Edición 0/0, 2020, ISSN 0947-0875
Editor: Spektrum Akademischer Verlag GmbH
DOI: 10.1515/nf-2020-0001

Large colloidal probes for atomic force microscopy: Fabrication and calibration issues

Autores: Matteo Chighizola, Luca Puricelli, Ludovic Bellon, Alessandro Podestà
Publicado en: Journal of Molecular Recognition, Edición 34/1, 2021, ISSN 0952-3499
Editor: John Wiley & Sons Inc.
DOI: 10.1002/jmr.2879

αvβ3 integrin expression increases elasticity in human melanoma cells

Autores: Leda Lacaria, Janina R. Lange, Wolfgang H. Goldmann, Felix Rico, José Luis Alonso
Publicado en: Biochemical and Biophysical Research Communications, Edición 525/4, 2020, Página(s) 836-840, ISSN 0006-291X
Editor: Academic Press
DOI: 10.1016/j.bbrc.2020.02.156

Force sensing on cells and tissues by atomic force microscopy

Autores: Hatice Holuigue; Ewelina Lorenc; Matteo Chighizola; Carsten Schulte; Luca Varinelli; Marcello Deraco; Marcello Guaglio; Manuela Gariboldi; Alessandro Podestà
Publicado en: Sensors, Edición 22(6), 2022, Página(s) 2197, ISSN 1424-8220
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s22062197

Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution

Autores: Massimiliano Berardi, Kevin Bielawski, Niek Rijnveld, Grzegorz Gruca, Hilde Aardema, Leni van Tol, Gijs Wuite, B. Imran Akca
Publicado en: Communications Biology, 2021, ISSN 2399-3642
Editor: Nature Portfolio
DOI: 10.1038/s42003-021-02121-1

Monographic books (1)

MECHANICS OF CELLS AND TISSUES IN DISEASES. Vol. 1: Biomedical Methods. Vol. 2: Biomedical Applications.

Autores: Edited by: M. Lekka, D. Navajas, M. Radmacher, A. Podestà
Publicado en: MECHANICS OF CELLS AND TISSUES IN DISEASES, 2023
Editor: De Gruyter

Other (3)

NOVEL NATIVE EXTRACELLULAR MATRIX PROBES TO TARGET PATIENT- AND TISSUE- SPECIFIC CELL-MICROENVIRONMENT INTERACTIONS BY FORCE SPECTROSCOPY

Autores: H. Holuigue, L. Nacci, P. Di Chiaro, M. Chighizola, I. Locatelli, C. Schulte, M. Alfano, G.R. Diaferia, A. Podestà
Publicado en: bioRxiv, 2022
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.12.02.518867

The nanomechanical fingerprint of colorectal cancer -derived peritoneal metastasis

Autores: E. Lorenc, L. Varinelli, M. Chighizola, S. Brich, F. Pisati, M. Guaglio, D. Baratti, M. Deraco, M. Gariboldi,A. Podestà
Publicado en: bioRxiv, 2022
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.08.17.504271

Coupled mechanical mapping and interference contrast microscopy reveal viscoelastic and adhesion hallmarks of monocytes differentiation into macrophages

Autores: Mar Eroles, Javier Lopez-Alonso, Alexandre Ortega, Thomas Boudier, Khaldoun Gharzeddine, Frank Lafont, Clemens M. Franz, Arnaud Millet, Claire Valoteau, Felix Rico
Publicado en: BioRxiv, 2022
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.11.29.518356

Derechos de propiedad intelectual

Patent (1)

OPTICAL SENSING DEVICE FOR MEASURING DEFORMATION OF A SAMPLE

Número de solicitud/publicación: 20 22054291
Fecha: 2022-02-21
Solicitante(s): OPTICS11 BV