Biomechanics in health and disease: advanced physical tools for innovative early diagnosis

Risultati finali

Progress Report

Preparation of the Progress Report.

Standardised procedure for preparation of tissues for mechanical testing

Definition of standardised procedures for the preparation of tissues for testing their mechanical properties.

Standardised cell culture conditions for mechanical testing

Definition of standardised cell culture conditions for mechanical testing.

Standardised procedure for tissue mechanics

Definition of standardised procedures for the characterisation of tissue mechanics.

Standardised procedure for immobilisation of tissues for mechanical testing

Definition of standardised procedures for the immobilisation of tissues for testing their mechanical properties.

Standardised procedure for cell mechanics

Development of standardised procedures for performing cell mechanics experiments.

Workshops

Organisation of Workshops, planned for Months 6,18,30,42.

Organisation of secondments

Organisation of secondments of ESRs (from Month 2 to Month 48).

Accuracy of mechanical tests

Determine the accuracy of mechanical tests, and identify the best measurement conditions.

Project meetings

Project meetings are planned at months 2,6,12,18,24,30,36,42,48.

Online resources

Management of online resources: wiki, webpage, social networks.

Contribution of ESRs to workshops, schools, and international conferences

Contribution of ESRs to workshops, schools, and international conferences. Planned roughly 6-monthly, starting from Month 6.

Recruitment of ESRs and PhD registration

Recruitment of ESRs and registration into the PhD programme.

Supervisory Board

Definition of the Supervisory Board of the network.

Identification of mechanical markers of diseases

Identification of specific mechanical markers of selected diseases.

Identification of links between biomechanics and disease

Identification of general links between biomechanics and disease.

Schools

Organisation of Schools, planned at Months 12, 24, 36.

Definition of secondments plan

All secondments plans must be prepared within this date.

Viscoelasticity of cells by microfluidics

Development of characterisation strategies of viscoelasticity of cells by microfluidic tools (beyond AFM).

Standardised procedures for sample shipping

Development of standardised procedures for cells and tissues transport and shipping.

Nano-biomechanics for schoolchildren

Nano-biomechanics communicated to schoolchildren (roughly annual).

Universal file format

Definition of a common universal file format for saving and sharing mechanical data in different laboratories.

Social networking

Social networking: creation and maintenance of Twitter, LinkedIn, ORCID and ResearchID accounts.

Open access to raw data

Deposition of raw data in open access repository according to ORDP rules

Website

Project website running.

Pubblicazioni

Novel Decellularization Method for Tissue Slices

Autori: Narciso M, Ulldemolins A, Júnior C, Otero J, Navajas D, Farré R, Gavara N, Almendros I
Pubblicato in: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Editore: Frontiers Media S.A.
DOI: 10.3389/fbioe.2022.832178

Image-Based Method to Quantify Decellularization of Tissue Sections.

Autori: Maria Narciso; Jorge Otero; Daniel Navajas; Ramon Farré; Isaac Almendros; Núria Gavara
Pubblicato in: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22168399

Traction force microscopy – Measuring the forces exerted by cells

Autori: Malgorzata Lekka, Kajangi Gnanachandran, Andrzej Kubiak, Tomasz Zielinski, Joanna Zemla
Pubblicato in: Micron, Numero 150, 2021, Pagina/e 103138, ISSN 0968-4328
Editore: Pergamon Press Ltd.
DOI: 10.1016/j.micron.2021.103138

Decellularized extracellular matrix as scaffold for cancer organoid cultures of colorectal peritoneal metastases

Autori: Luca Varinelli, Marcello Guaglio, Silvia Brich, Susanna Zanutto, Antonino Belfiore, Federica Zanardi, Fabio Iannelli, Amanda Oldani, Elisa Costa, Matteo Chighizola, Ewelina Lorenc, Simone P Minardi, Stefano Fortuzzi, Martina Filugelli, Giovanna Garzone, Federica Pisati, Manuela Vecchi, Giancarlo Pruneri, Shigeki Kusamura, Dario Baratti, Laura Cattaneo, Dario Parazzoli, Alessandro Podestà, Massimo
Pubblicato in: Journal of Molecular Cell Biology, 2022, ISSN 1759-4685
Editore: Oxford University Press
DOI: 10.1093/jmcb/mjac064

Mutated lamin A modulates stiffness in muscle cells

Autori: Maria Chatzifrangkeskou, Delf Kah, Janina R. Lange, Wolfgang H. Goldmann, Antoine Muchir
Pubblicato in: Biochemical and Biophysical Research Communications, Numero 529/3, 2020, Pagina/e 861-867, ISSN 0006-291X
Editore: Academic Press
DOI: 10.1016/j.bbrc.2020.05.102

Silk-Reinforced Collagen Hydrogels with Raised Multiscale Stiffness for Mesenchymal Cells 3D Culture

Autori: Sanz-Fraile, H., Amoros, S., Mendizabal, I., Galvez-Monton, C., Prat-Vidal, C., Bayes-Genis, A., Navajas, D., Farre, R., & Otero, J.
Pubblicato in: Tissue Engineering Part A, 2020, ISSN 1937-335X
Editore: Mary Ann Liebert Inc.
DOI: 10.1089/ten.tea.2019.0199

Applicability of atomic force microscopy to determine cancer-related changes in cells

Autori: Malgorzata Lekka
Pubblicato in: Phil. Trans. R. Soc. A, 2022, ISSN 1471-2962
Editore: The Royal Society publishing
DOI: 10.1098/rsta.2021.0346

The glycocalyx affects the mechanotransductive perception of the topographical microenvironment

Autori: Matteo Chighizola; Tania Dini; Stefania Marcotti; Mirko D’Urso; Claudio Piazzoni; Francesca Borghi; Anita Previdi; Laura Ceriani; Claudia Folliero; Brian Stramer; Cristina Lenardi; Paolo Milani; Alessandro Podestà; Carsten Schulte
Pubblicato in: Journal of Nanobiotechnology, 2022, Pagina/e 418, ISSN 1477-3155
Editore: BioMed Central
DOI: 10.1186/s12951-022-01585-5

Universal Model for the Log-Normal Distribution of Elasticity in Polymeric Gels and Its Relevance to Mechanical Signature of Biological Tissues.

Autori: Arnaud Millet
Pubblicato in: Biology (Basel), Numero 10(1), 2021, Pagina/e 64, ISSN 2079-7737
Editore: Biology
DOI: 10.3390/biology10010064

Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells

Autori: Falcones B, Sanz-Fraile H, Marhuenda E, Mendizábal I, Cabrera-Aguilera I, Malandain N, Uriarte JJ, Almendros I, Navajas D, Weiss DJ, Farré R, Otero J.
Pubblicato in: Polymers, 2021, ISSN 2073-4360
Editore: MDPI AG
DOI: 10.3390/polym13142350

Nano-structural stiness measure for soft biomaterials of heterogeneous elasticity

Autori: Shu-wen W. Chen, Jean-Marie Teulon, Harinderbir Kaur, Christian Godon and Jean-Luc Pellequer
Pubblicato in: Nanoscale Horizons, Numero 20556764, 2022, ISSN 2055-6764
Editore: Royal Society of Chemistry
DOI: 10.1039/d2nh00390b

A Fast and Efficient Decellularization Method for Tissue Slices

Autori: Narciso M, Ulldemolins A, Júnior C, Otero J, Navajas D, Farré R, Gavara N, Almendros I
Pubblicato in: Bio-protocol Journal, 2022, ISSN 2331-8325
Editore: Sunnyvale CA: Bio-protocol LLC
DOI: 10.21769/bioprotoc.4550

Viscoelastic properties of suspended cells measured with shear flow deformation cytometry

Autori: Richard Gerum; Elham Mirzahossein; Mar Eroles; Jennifer Elsterer; Astrid Mainka; Andreas Bauer; Selina Sonntag; Alexander Winterl; Johannes Bartl; Lena Fischer; Shada Abuhattum; Ruchi Goswami; Salvatore Girardo; Jochen Guck; Stefan Schrüfer; Nadine Ströhlein; Mojtaba Nosratlo; Harald Herrmann; Dorothea Schultheis; Felix Rico; Sebastian Müller; Stephan Gekle; Ben Fabry
Pubblicato in: eLife, 2022, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.78823

Discriminating bladder cancer cells through rheological mechanomarkers at cell and spheroid levels

Autori: Kajangi Gnanachandran, Sylwia Kędracka-Krok, Joanna Pabijan, Małgorzata Lekka
Pubblicato in: Journal of Biomechanics, Numero 144, 2022, Pagina/e 111346, ISSN 0021-9290
Editore: Elsevier BV
DOI: 10.1016/j.jbiomech.2022.111346

Mechanical modeling of lung alveoli: From macroscopic behaviour to cell mechano-sensing at microscopic level

Autori: Daniel Navajas; José Manuel Garcia-Aznar; Gabriel Beltran
Pubblicato in: Journal of the Mechanical Behavior of Biomedical Materials, 2022, ISSN 1751-6161
Editore: Elsevier BV
DOI: 10.1016/j.jmbbm.2021.105043

Dynamic mechanical analysis of suspended soft bodies via hydraulic force spectroscopy

Autori: Massimiliano Berardi, Kajangi Gnanachandran, Jieke Jiang, Kevin Bielawski, Claas W. Visser, Małgorzata Lekka, B. Imran akca
Pubblicato in: Soft Matter, 2022, ISSN 1744-683X
Editore: Royal Society of Chemistry
DOI: 10.1039/d2sm01173e

Hypoxia Drives Dihydropyrimidine Dehydrogenase Expression in Macrophages and Confers Chemoresistance in Colorectal Cancer

Autori: Malier M, Gharzeddine K, Laverriere MH, Marsili S, Thomas F, Court M, Decaens T, Roth G, Millet A.
Pubblicato in: Cancer research, 2021, ISSN 0008-5472
Editore: American Association for Cancer Research
DOI: 10.1158/0008-5472.can-21-1572

Involvement of Mechanical Cues in the Migration of Cajal-Retzius Cells in the Marginal Zone During Neocortical Development

Autori: López-Mengual A, Segura-Feliu M, Sunyer R, Sanz-Fraile H, Otero J, Mesquida-Veny F, Gil V, Hervera A, Ferrer I, Soriano J, Trepat X, Farré R, Navajas D, Del Río JA
Pubblicato in: Frontiers in Cell and Developmental Biology, 2022, ISSN 2296-634X
Editore: Frontiers Media S.A.
DOI: 10.3389/fcell.2022.886110

Human Septins Organize as Octamer-Based Filaments and Mediate Actin-Membrane Anchoring in Cells.

Autori: Martins C. S., Taveneau C., Castro-Linares G., Baibakov M., Buzhinsky N., Eroles M., Milanović V., Omi S., Pedelacq J.-D., Iv F., Bouillard L., Llewellyn A., Gomes M., Belhabib M., Kuzmić M., Verdier-Pinard P., Lee S., Badache A., Kumar S., Chandre C., Brasselet S., Rico F., Rossier O., Koenderink G. H., Wenger J., Cabantous S., Mavrakis M.
Pubblicato in: Journal of Cell Biology, 2023, ISSN 0021-9525
Editore: Rockefeller University Press
DOI: 10.1083/jcb.202203016

Baseline Stiffness Modulates the Non-Linear Response to Stretch of the Extracellular Matrix in Pulmonary Fibrosis

Autori: Júnior C, Narciso M, Marhuenda E, Almendros I, Farré R, Navajas D, Otero J, Gavara N
Pubblicato in: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms222312928

Adhesion force spectroscopy with nanostructured colloidal probes reveals nanotopography-dependent early mechanotransductive interactions at the cell membrane level

Autori: M. Chighizola, A. Previdi, T. Dini, C. Piazzoni, C. Lenardi, P. Milani, C. Schulte, A. Podestà
Pubblicato in: Nanoscale, Numero 12/27, 2020, Pagina/e 14708-14723, ISSN 2040-3364
Editore: Royal Society of Chemistry
DOI: 10.1039/d0nr01991g

Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation System

Autori: Lolo F-N, Walani N, Seemann E, Zalvidea D, Pavón DM, Cojoc G, Zamai M, Viaris de Lesegno C, Martínez de Benito F, Sánchez-Álvarez M, Uriarte JJ, Echarri A, Escolano J-C, Sánchez SA, Caiolfa VR, Navajas D, Trepat X, Guck J, Lamaze C, Roca-Cusachs P, Kessels MM, Qualmann B, Arroyo M, del Pozo MA.
Pubblicato in: Nat Cell Biol., Numero 25, 2023, Pagina/e 120-133, ISSN 1465-7392
Editore: Nature Publishing Group
DOI: 10.1038/s41556-022-01034-3

Multi-step Extracellular Matrix Remodelling and Stiffening in the Development of Idiopathic Pulmonary Fibrosis

Autori: Júnior C, Ulldemolins A, Narciso M, Almendros I, Farre R, Navajas D, Lopez J, Eroles M, Rico F, Gavara N
Pubblicato in: Int J Mol Sci, Numero 24, 2023, Pagina/e 1708, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24021708

Stiffening of DU145 prostate cancer cells driven by actin filaments - microtubule crosstalk conferring resistance to microtubule-targeting drugs.

Autori: Andrzej Kubiak; Matteo Chighizola; Carsten Schulte; Natalia Bryniarska; Julita Wesołowska; Maciej Pudełek; Małgorzata Lasota; Damian Ryszawy; Agnieszka Basta-Kaim; Piotr Laidler; Alessandro Podestà; Małgorzata Lekka
Pubblicato in: Nanoscale, 2021, ISSN 2040-3372
Editore: Royal Society of Chemistry
DOI: 10.1039/d0nr06464e

Mechanotransduction in neuronal cell development and functioning

Autori: Matteo Chighizola, Tania Dini, Cristina Lenardi, Paolo Milani, Alessandro Podestà, Carsten Schulte
Pubblicato in: Biophysical Reviews, Numero 11/5, 2019, Pagina/e 701-720, ISSN 1867-2450
Editore: Springer Verlag
DOI: 10.1007/s12551-019-00587-2

Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy

Autori: Katerina Karkali, Ignasi Jorba, Daniel Navajas, Enrique Martin-Blanco
Pubblicato in: STAR protocols, Numero 3, 2022, Pagina/e 101901, ISSN 2666-1667
Editore: Cell Press
DOI: 10.1016/j.xpro.2022.101901

Rheological comparison between control and Dupuytren fibroblasts when plated in circular micropatterns using atomic force microscopy

Autori: Sandra Pérez-Domínguez, Elisabeth Werkmeister, Maria Luisa Marini, Vicent Dupres, Sébastien Janel, Frank Lafont, Manfred Radmacher
Pubblicato in: Frontiers in Physics, Sec. Biophysics, 2022, ISSN 2296-424X
Editore: Frontiers Media
DOI: 10.3389/fphy.2022.1052203

Comparison of rheological properties of healthy versus Dupuytren fibroblasts when treated with a cell contraction inhibitor by Atomic Force Microscope

Autori: Sandra Pérez-Domínguez, Javier López-Alonso, Frank Lafont and Manfred Radmacher
Pubblicato in: International Journal of Molecular Science, 2023, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24032043

Micro-mechanical fingerprints of the rat bladder change in actinic cystitis and tumor presence

Autori: L. Martinez-Vidal, M. Chighizola, M. Berardi, E. Alchera, I. Locatelli, F. Pederzoli, C. Venegoni, R. Lucianò, P. Milani, K. Bielawski, A. Salonia, A. Podestà, M. Alfano
Pubblicato in: Communications Biology, 2023, ISSN 2399-3642
Editore: Springer Nature
DOI: 10.1038/s42003-023-04572-0

Atomic force microscopy for cell mechanics and diseases

Autori: Sandra Pérez-Domínguez, Shruti G. Kulkarni, Carmela Rianna, Manfred Radmacher
Pubblicato in: Neuroforum, Numero 0/0, 2020, ISSN 0947-0875
Editore: Spektrum Akademischer Verlag GmbH
DOI: 10.1515/nf-2020-0001

Large colloidal probes for atomic force microscopy: Fabrication and calibration issues

Autori: Matteo Chighizola, Luca Puricelli, Ludovic Bellon, Alessandro Podestà
Pubblicato in: Journal of Molecular Recognition, Numero 34/1, 2021, ISSN 0952-3499
Editore: John Wiley & Sons Inc.
DOI: 10.1002/jmr.2879

αvβ3 integrin expression increases elasticity in human melanoma cells

Autori: Leda Lacaria, Janina R. Lange, Wolfgang H. Goldmann, Felix Rico, José Luis Alonso
Pubblicato in: Biochemical and Biophysical Research Communications, Numero 525/4, 2020, Pagina/e 836-840, ISSN 0006-291X
Editore: Academic Press
DOI: 10.1016/j.bbrc.2020.02.156

Force sensing on cells and tissues by atomic force microscopy

Autori: Hatice Holuigue; Ewelina Lorenc; Matteo Chighizola; Carsten Schulte; Luca Varinelli; Marcello Deraco; Marcello Guaglio; Manuela Gariboldi; Alessandro Podestà
Pubblicato in: Sensors, Numero 22(6), 2022, Pagina/e 2197, ISSN 1424-8220
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s22062197

Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution

Autori: Massimiliano Berardi, Kevin Bielawski, Niek Rijnveld, Grzegorz Gruca, Hilde Aardema, Leni van Tol, Gijs Wuite, B. Imran Akca
Pubblicato in: Communications Biology, 2021, ISSN 2399-3642
Editore: Nature Portfolio
DOI: 10.1038/s42003-021-02121-1

MECHANICS OF CELLS AND TISSUES IN DISEASES. Vol. 1: Biomedical Methods. Vol. 2: Biomedical Applications.

Autori: Edited by: M. Lekka, D. Navajas, M. Radmacher, A. Podestà
Pubblicato in: MECHANICS OF CELLS AND TISSUES IN DISEASES, 2023
Editore: De Gruyter

NOVEL NATIVE EXTRACELLULAR MATRIX PROBES TO TARGET PATIENT- AND TISSUE- SPECIFIC CELL-MICROENVIRONMENT INTERACTIONS BY FORCE SPECTROSCOPY

Autori: H. Holuigue, L. Nacci, P. Di Chiaro, M. Chighizola, I. Locatelli, C. Schulte, M. Alfano, G.R. Diaferia, A. Podestà
Pubblicato in: bioRxiv, 2022
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.12.02.518867

The nanomechanical fingerprint of colorectal cancer -derived peritoneal metastasis

Autori: E. Lorenc, L. Varinelli, M. Chighizola, S. Brich, F. Pisati, M. Guaglio, D. Baratti, M. Deraco, M. Gariboldi,A. Podestà
Pubblicato in: bioRxiv, 2022
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.08.17.504271

Coupled mechanical mapping and interference contrast microscopy reveal viscoelastic and adhesion hallmarks of monocytes differentiation into macrophages

Autori: Mar Eroles, Javier Lopez-Alonso, Alexandre Ortega, Thomas Boudier, Khaldoun Gharzeddine, Frank Lafont, Clemens M. Franz, Arnaud Millet, Claire Valoteau, Felix Rico
Pubblicato in: BioRxiv, 2022
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.11.29.518356

Diritti di proprietà intellettuale

OPTICAL SENSING DEVICE FOR MEASURING DEFORMATION OF A SAMPLE

Numero candidatura/pubblicazione: 20 22054291
Data: 2022-02-21
Candidato/i: OPTICS11 BV

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