Biomechanics in health and disease: advanced physical tools for innovative early diagnosis

Leistungen

Progress Report

Preparation of the Progress Report.

Standardised procedure for preparation of tissues for mechanical testing

Definition of standardised procedures for the preparation of tissues for testing their mechanical properties.

Standardised cell culture conditions for mechanical testing

Definition of standardised cell culture conditions for mechanical testing.

Standardised procedure for tissue mechanics

Definition of standardised procedures for the characterisation of tissue mechanics.

Standardised procedure for immobilisation of tissues for mechanical testing

Definition of standardised procedures for the immobilisation of tissues for testing their mechanical properties.

Standardised procedure for cell mechanics

Development of standardised procedures for performing cell mechanics experiments.

Workshops

Organisation of Workshops, planned for Months 6,18,30,42.

Organisation of secondments

Organisation of secondments of ESRs (from Month 2 to Month 48).

Accuracy of mechanical tests

Determine the accuracy of mechanical tests, and identify the best measurement conditions.

Project meetings

Project meetings are planned at months 2,6,12,18,24,30,36,42,48.

Online resources

Management of online resources: wiki, webpage, social networks.

Contribution of ESRs to workshops, schools, and international conferences

Contribution of ESRs to workshops, schools, and international conferences. Planned roughly 6-monthly, starting from Month 6.

Recruitment of ESRs and PhD registration

Recruitment of ESRs and registration into the PhD programme.

Supervisory Board

Definition of the Supervisory Board of the network.

Identification of mechanical markers of diseases

Identification of specific mechanical markers of selected diseases.

Identification of links between biomechanics and disease

Identification of general links between biomechanics and disease.

Schools

Organisation of Schools, planned at Months 12, 24, 36.

Definition of secondments plan

All secondments plans must be prepared within this date.

Viscoelasticity of cells by microfluidics

Development of characterisation strategies of viscoelasticity of cells by microfluidic tools (beyond AFM).

Standardised procedures for sample shipping

Development of standardised procedures for cells and tissues transport and shipping.

Nano-biomechanics for schoolchildren

Nano-biomechanics communicated to schoolchildren (roughly annual).

Universal file format

Definition of a common universal file format for saving and sharing mechanical data in different laboratories.

Social networking

Social networking: creation and maintenance of Twitter, LinkedIn, ORCID and ResearchID accounts.

Open access to raw data

Deposition of raw data in open access repository according to ORDP rules

Website

Project website running.

Veröffentlichungen

Novel Decellularization Method for Tissue Slices

Autoren: Narciso M, Ulldemolins A, Júnior C, Otero J, Navajas D, Farré R, Gavara N, Almendros I
Veröffentlicht in: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Herausgeber: Frontiers Media S.A.
DOI: 10.3389/fbioe.2022.832178

Image-Based Method to Quantify Decellularization of Tissue Sections.

Autoren: Maria Narciso; Jorge Otero; Daniel Navajas; Ramon Farré; Isaac Almendros; Núria Gavara
Veröffentlicht in: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22168399

Traction force microscopy – Measuring the forces exerted by cells

Autoren: Malgorzata Lekka, Kajangi Gnanachandran, Andrzej Kubiak, Tomasz Zielinski, Joanna Zemla
Veröffentlicht in: Micron, Ausgabe 150, 2021, Seite(n) 103138, ISSN 0968-4328
Herausgeber: Pergamon Press Ltd.
DOI: 10.1016/j.micron.2021.103138

Decellularized extracellular matrix as scaffold for cancer organoid cultures of colorectal peritoneal metastases

Autoren: Luca Varinelli, Marcello Guaglio, Silvia Brich, Susanna Zanutto, Antonino Belfiore, Federica Zanardi, Fabio Iannelli, Amanda Oldani, Elisa Costa, Matteo Chighizola, Ewelina Lorenc, Simone P Minardi, Stefano Fortuzzi, Martina Filugelli, Giovanna Garzone, Federica Pisati, Manuela Vecchi, Giancarlo Pruneri, Shigeki Kusamura, Dario Baratti, Laura Cattaneo, Dario Parazzoli, Alessandro Podestà, Massimo
Veröffentlicht in: Journal of Molecular Cell Biology, 2022, ISSN 1759-4685
Herausgeber: Oxford University Press
DOI: 10.1093/jmcb/mjac064

Mutated lamin A modulates stiffness in muscle cells

Autoren: Maria Chatzifrangkeskou, Delf Kah, Janina R. Lange, Wolfgang H. Goldmann, Antoine Muchir
Veröffentlicht in: Biochemical and Biophysical Research Communications, Ausgabe 529/3, 2020, Seite(n) 861-867, ISSN 0006-291X
Herausgeber: Academic Press
DOI: 10.1016/j.bbrc.2020.05.102

Silk-Reinforced Collagen Hydrogels with Raised Multiscale Stiffness for Mesenchymal Cells 3D Culture

Autoren: Sanz-Fraile, H., Amoros, S., Mendizabal, I., Galvez-Monton, C., Prat-Vidal, C., Bayes-Genis, A., Navajas, D., Farre, R., & Otero, J.
Veröffentlicht in: Tissue Engineering Part A, 2020, ISSN 1937-335X
Herausgeber: Mary Ann Liebert Inc.
DOI: 10.1089/ten.tea.2019.0199

Applicability of atomic force microscopy to determine cancer-related changes in cells

Autoren: Malgorzata Lekka
Veröffentlicht in: Phil. Trans. R. Soc. A, 2022, ISSN 1471-2962
Herausgeber: The Royal Society publishing
DOI: 10.1098/rsta.2021.0346

The glycocalyx affects the mechanotransductive perception of the topographical microenvironment

Autoren: Matteo Chighizola; Tania Dini; Stefania Marcotti; Mirko D’Urso; Claudio Piazzoni; Francesca Borghi; Anita Previdi; Laura Ceriani; Claudia Folliero; Brian Stramer; Cristina Lenardi; Paolo Milani; Alessandro Podestà; Carsten Schulte
Veröffentlicht in: Journal of Nanobiotechnology, 2022, Seite(n) 418, ISSN 1477-3155
Herausgeber: BioMed Central
DOI: 10.1186/s12951-022-01585-5

Universal Model for the Log-Normal Distribution of Elasticity in Polymeric Gels and Its Relevance to Mechanical Signature of Biological Tissues.

Autoren: Arnaud Millet
Veröffentlicht in: Biology (Basel), Ausgabe 10(1), 2021, Seite(n) 64, ISSN 2079-7737
Herausgeber: Biology
DOI: 10.3390/biology10010064

Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells

Autoren: Falcones B, Sanz-Fraile H, Marhuenda E, Mendizábal I, Cabrera-Aguilera I, Malandain N, Uriarte JJ, Almendros I, Navajas D, Weiss DJ, Farré R, Otero J.
Veröffentlicht in: Polymers, 2021, ISSN 2073-4360
Herausgeber: MDPI AG
DOI: 10.3390/polym13142350

Nano-structural stiness measure for soft biomaterials of heterogeneous elasticity

Autoren: Shu-wen W. Chen, Jean-Marie Teulon, Harinderbir Kaur, Christian Godon and Jean-Luc Pellequer
Veröffentlicht in: Nanoscale Horizons, Ausgabe 20556764, 2022, ISSN 2055-6764
Herausgeber: Royal Society of Chemistry
DOI: 10.1039/d2nh00390b

A Fast and Efficient Decellularization Method for Tissue Slices

Autoren: Narciso M, Ulldemolins A, Júnior C, Otero J, Navajas D, Farré R, Gavara N, Almendros I
Veröffentlicht in: Bio-protocol Journal, 2022, ISSN 2331-8325
Herausgeber: Sunnyvale CA: Bio-protocol LLC
DOI: 10.21769/bioprotoc.4550

Viscoelastic properties of suspended cells measured with shear flow deformation cytometry

Autoren: Richard Gerum; Elham Mirzahossein; Mar Eroles; Jennifer Elsterer; Astrid Mainka; Andreas Bauer; Selina Sonntag; Alexander Winterl; Johannes Bartl; Lena Fischer; Shada Abuhattum; Ruchi Goswami; Salvatore Girardo; Jochen Guck; Stefan Schrüfer; Nadine Ströhlein; Mojtaba Nosratlo; Harald Herrmann; Dorothea Schultheis; Felix Rico; Sebastian Müller; Stephan Gekle; Ben Fabry
Veröffentlicht in: eLife, 2022, ISSN 2050-084X
Herausgeber: eLife Sciences Publications
DOI: 10.7554/elife.78823

Discriminating bladder cancer cells through rheological mechanomarkers at cell and spheroid levels

Autoren: Kajangi Gnanachandran, Sylwia Kędracka-Krok, Joanna Pabijan, Małgorzata Lekka
Veröffentlicht in: Journal of Biomechanics, Ausgabe 144, 2022, Seite(n) 111346, ISSN 0021-9290
Herausgeber: Elsevier BV
DOI: 10.1016/j.jbiomech.2022.111346

Mechanical modeling of lung alveoli: From macroscopic behaviour to cell mechano-sensing at microscopic level

Autoren: Daniel Navajas; José Manuel Garcia-Aznar; Gabriel Beltran
Veröffentlicht in: Journal of the Mechanical Behavior of Biomedical Materials, 2022, ISSN 1751-6161
Herausgeber: Elsevier BV
DOI: 10.1016/j.jmbbm.2021.105043

Dynamic mechanical analysis of suspended soft bodies via hydraulic force spectroscopy

Autoren: Massimiliano Berardi, Kajangi Gnanachandran, Jieke Jiang, Kevin Bielawski, Claas W. Visser, Małgorzata Lekka, B. Imran akca
Veröffentlicht in: Soft Matter, 2022, ISSN 1744-683X
Herausgeber: Royal Society of Chemistry
DOI: 10.1039/d2sm01173e

Hypoxia Drives Dihydropyrimidine Dehydrogenase Expression in Macrophages and Confers Chemoresistance in Colorectal Cancer

Autoren: Malier M, Gharzeddine K, Laverriere MH, Marsili S, Thomas F, Court M, Decaens T, Roth G, Millet A.
Veröffentlicht in: Cancer research, 2021, ISSN 0008-5472
Herausgeber: American Association for Cancer Research
DOI: 10.1158/0008-5472.can-21-1572

Involvement of Mechanical Cues in the Migration of Cajal-Retzius Cells in the Marginal Zone During Neocortical Development

Autoren: López-Mengual A, Segura-Feliu M, Sunyer R, Sanz-Fraile H, Otero J, Mesquida-Veny F, Gil V, Hervera A, Ferrer I, Soriano J, Trepat X, Farré R, Navajas D, Del Río JA
Veröffentlicht in: Frontiers in Cell and Developmental Biology, 2022, ISSN 2296-634X
Herausgeber: Frontiers Media S.A.
DOI: 10.3389/fcell.2022.886110

Human Septins Organize as Octamer-Based Filaments and Mediate Actin-Membrane Anchoring in Cells.

Autoren: Martins C. S., Taveneau C., Castro-Linares G., Baibakov M., Buzhinsky N., Eroles M., Milanović V., Omi S., Pedelacq J.-D., Iv F., Bouillard L., Llewellyn A., Gomes M., Belhabib M., Kuzmić M., Verdier-Pinard P., Lee S., Badache A., Kumar S., Chandre C., Brasselet S., Rico F., Rossier O., Koenderink G. H., Wenger J., Cabantous S., Mavrakis M.
Veröffentlicht in: Journal of Cell Biology, 2023, ISSN 0021-9525
Herausgeber: Rockefeller University Press
DOI: 10.1083/jcb.202203016

Baseline Stiffness Modulates the Non-Linear Response to Stretch of the Extracellular Matrix in Pulmonary Fibrosis

Autoren: Júnior C, Narciso M, Marhuenda E, Almendros I, Farré R, Navajas D, Otero J, Gavara N
Veröffentlicht in: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms222312928

Adhesion force spectroscopy with nanostructured colloidal probes reveals nanotopography-dependent early mechanotransductive interactions at the cell membrane level

Autoren: M. Chighizola, A. Previdi, T. Dini, C. Piazzoni, C. Lenardi, P. Milani, C. Schulte, A. Podestà
Veröffentlicht in: Nanoscale, Ausgabe 12/27, 2020, Seite(n) 14708-14723, ISSN 2040-3364
Herausgeber: Royal Society of Chemistry
DOI: 10.1039/d0nr01991g

Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation System

Autoren: Lolo F-N, Walani N, Seemann E, Zalvidea D, Pavón DM, Cojoc G, Zamai M, Viaris de Lesegno C, Martínez de Benito F, Sánchez-Álvarez M, Uriarte JJ, Echarri A, Escolano J-C, Sánchez SA, Caiolfa VR, Navajas D, Trepat X, Guck J, Lamaze C, Roca-Cusachs P, Kessels MM, Qualmann B, Arroyo M, del Pozo MA.
Veröffentlicht in: Nat Cell Biol., Ausgabe 25, 2023, Seite(n) 120-133, ISSN 1465-7392
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41556-022-01034-3

Multi-step Extracellular Matrix Remodelling and Stiffening in the Development of Idiopathic Pulmonary Fibrosis

Autoren: Júnior C, Ulldemolins A, Narciso M, Almendros I, Farre R, Navajas D, Lopez J, Eroles M, Rico F, Gavara N
Veröffentlicht in: Int J Mol Sci, Ausgabe 24, 2023, Seite(n) 1708, ISSN 1422-0067
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24021708

Stiffening of DU145 prostate cancer cells driven by actin filaments - microtubule crosstalk conferring resistance to microtubule-targeting drugs.

Autoren: Andrzej Kubiak; Matteo Chighizola; Carsten Schulte; Natalia Bryniarska; Julita Wesołowska; Maciej Pudełek; Małgorzata Lasota; Damian Ryszawy; Agnieszka Basta-Kaim; Piotr Laidler; Alessandro Podestà; Małgorzata Lekka
Veröffentlicht in: Nanoscale, 2021, ISSN 2040-3372
Herausgeber: Royal Society of Chemistry
DOI: 10.1039/d0nr06464e

Mechanotransduction in neuronal cell development and functioning

Autoren: Matteo Chighizola, Tania Dini, Cristina Lenardi, Paolo Milani, Alessandro Podestà, Carsten Schulte
Veröffentlicht in: Biophysical Reviews, Ausgabe 11/5, 2019, Seite(n) 701-720, ISSN 1867-2450
Herausgeber: Springer Verlag
DOI: 10.1007/s12551-019-00587-2

Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy

Autoren: Katerina Karkali, Ignasi Jorba, Daniel Navajas, Enrique Martin-Blanco
Veröffentlicht in: STAR protocols, Ausgabe 3, 2022, Seite(n) 101901, ISSN 2666-1667
Herausgeber: Cell Press
DOI: 10.1016/j.xpro.2022.101901

Rheological comparison between control and Dupuytren fibroblasts when plated in circular micropatterns using atomic force microscopy

Autoren: Sandra Pérez-Domínguez, Elisabeth Werkmeister, Maria Luisa Marini, Vicent Dupres, Sébastien Janel, Frank Lafont, Manfred Radmacher
Veröffentlicht in: Frontiers in Physics, Sec. Biophysics, 2022, ISSN 2296-424X
Herausgeber: Frontiers Media
DOI: 10.3389/fphy.2022.1052203

Comparison of rheological properties of healthy versus Dupuytren fibroblasts when treated with a cell contraction inhibitor by Atomic Force Microscope

Autoren: Sandra Pérez-Domínguez, Javier López-Alonso, Frank Lafont and Manfred Radmacher
Veröffentlicht in: International Journal of Molecular Science, 2023, ISSN 1422-0067
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24032043

Micro-mechanical fingerprints of the rat bladder change in actinic cystitis and tumor presence

Autoren: L. Martinez-Vidal, M. Chighizola, M. Berardi, E. Alchera, I. Locatelli, F. Pederzoli, C. Venegoni, R. Lucianò, P. Milani, K. Bielawski, A. Salonia, A. Podestà, M. Alfano
Veröffentlicht in: Communications Biology, 2023, ISSN 2399-3642
Herausgeber: Springer Nature
DOI: 10.1038/s42003-023-04572-0

Atomic force microscopy for cell mechanics and diseases

Autoren: Sandra Pérez-Domínguez, Shruti G. Kulkarni, Carmela Rianna, Manfred Radmacher
Veröffentlicht in: Neuroforum, Ausgabe 0/0, 2020, ISSN 0947-0875
Herausgeber: Spektrum Akademischer Verlag GmbH
DOI: 10.1515/nf-2020-0001

Large colloidal probes for atomic force microscopy: Fabrication and calibration issues

Autoren: Matteo Chighizola, Luca Puricelli, Ludovic Bellon, Alessandro Podestà
Veröffentlicht in: Journal of Molecular Recognition, Ausgabe 34/1, 2021, ISSN 0952-3499
Herausgeber: John Wiley & Sons Inc.
DOI: 10.1002/jmr.2879

αvβ3 integrin expression increases elasticity in human melanoma cells

Autoren: Leda Lacaria, Janina R. Lange, Wolfgang H. Goldmann, Felix Rico, José Luis Alonso
Veröffentlicht in: Biochemical and Biophysical Research Communications, Ausgabe 525/4, 2020, Seite(n) 836-840, ISSN 0006-291X
Herausgeber: Academic Press
DOI: 10.1016/j.bbrc.2020.02.156

Force sensing on cells and tissues by atomic force microscopy

Autoren: Hatice Holuigue; Ewelina Lorenc; Matteo Chighizola; Carsten Schulte; Luca Varinelli; Marcello Deraco; Marcello Guaglio; Manuela Gariboldi; Alessandro Podestà
Veröffentlicht in: Sensors, Ausgabe 22(6), 2022, Seite(n) 2197, ISSN 1424-8220
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s22062197

Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution

Autoren: Massimiliano Berardi, Kevin Bielawski, Niek Rijnveld, Grzegorz Gruca, Hilde Aardema, Leni van Tol, Gijs Wuite, B. Imran Akca
Veröffentlicht in: Communications Biology, 2021, ISSN 2399-3642
Herausgeber: Nature Portfolio
DOI: 10.1038/s42003-021-02121-1

MECHANICS OF CELLS AND TISSUES IN DISEASES. Vol. 1: Biomedical Methods. Vol. 2: Biomedical Applications.

Autoren: Edited by: M. Lekka, D. Navajas, M. Radmacher, A. Podestà
Veröffentlicht in: MECHANICS OF CELLS AND TISSUES IN DISEASES, 2023
Herausgeber: De Gruyter

NOVEL NATIVE EXTRACELLULAR MATRIX PROBES TO TARGET PATIENT- AND TISSUE- SPECIFIC CELL-MICROENVIRONMENT INTERACTIONS BY FORCE SPECTROSCOPY

Autoren: H. Holuigue, L. Nacci, P. Di Chiaro, M. Chighizola, I. Locatelli, C. Schulte, M. Alfano, G.R. Diaferia, A. Podestà
Veröffentlicht in: bioRxiv, 2022
Herausgeber: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.12.02.518867

The nanomechanical fingerprint of colorectal cancer -derived peritoneal metastasis

Autoren: E. Lorenc, L. Varinelli, M. Chighizola, S. Brich, F. Pisati, M. Guaglio, D. Baratti, M. Deraco, M. Gariboldi,A. Podestà
Veröffentlicht in: bioRxiv, 2022
Herausgeber: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.08.17.504271

Coupled mechanical mapping and interference contrast microscopy reveal viscoelastic and adhesion hallmarks of monocytes differentiation into macrophages

Autoren: Mar Eroles, Javier Lopez-Alonso, Alexandre Ortega, Thomas Boudier, Khaldoun Gharzeddine, Frank Lafont, Clemens M. Franz, Arnaud Millet, Claire Valoteau, Felix Rico
Veröffentlicht in: BioRxiv, 2022
Herausgeber: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.11.29.518356

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OPTICAL SENSING DEVICE FOR MEASURING DEFORMATION OF A SAMPLE

Antrags-/Publikationsnummer: 20 22054291
Datum: 2022-02-21
Antragsteller: OPTICS11 BV

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