Projektbeschreibung
Mikrobielle Zellfabriken für Fluorchemikalien
In unserer modernen Welt sind fluorhaltige Materialien extrem wichtig. Anwendungen in den Bereichen Elektronik, Gesundheitsversorgung, im Fahrzeugsektor und im Zusammenhang mit tragbaren Technologien sind gängige Praxis. Gegenwärtig werden diese Fluorchemikalien ausschließlich mit aggressiven chemischen Verfahren synthetisiert. Das EU-finanzierte Projekt SinFonia zielt nun auf die Entwicklung einer nachhaltigeren Alternative ab. In diesem Sinne soll das robuste Bakterium Pseudomonas putida zur Zellfabrik werden, die fluorierte Monomere und Polymere herstellt. Das Projekt wird in diesen Bakterien mithilfe des Metabolic Engineering Fluorchemikalien herstellen, wobei die in der Industrie traditionell üblichen chemischen Reaktionen umgangen werden. Zielverbindungen sind eine ganze Familie fluorierter Polyester mit verbesserten physikalisch-chemischen und materialtypischen Eigenschaften, die für den Einsatz im Zusammenhang mit selbstreinigenden Oberflächen, Beschichtungen mit geringer Oberflächenenergie, biobasierten Schmierstoffen, Brennstoffzell-Membranen und bewuchshemmenden Materialien vorgesehen sind.
Ziel
Nature has hardly evolved biochemical reactions involving fluorine (F), the most abundant halogen on Earth. Organic compounds containing F (fluorochemicals) are, however, extremely relevant from an industrial point of view. Fluoropolymers are the main fluorochemicals in the market worldwide, and are exclusively synthesized using chemical methods. Moreover, current fluorination technologies usually involve corrosive and toxic reagents that have a negative impact on the environment. Designing sustainable bioprocesses based on alternative and safer fluorinating agents from renewable substrates is thus a long-sought-after, yet unfulfilled goal. SinFonia proposes to engineer the metabolically-versatile bacterium Pseudomonas putida to execute biofluorinations for generating novel fluoropolymers from renewable substrates. P. putida KT2440, a non-pathogenic soil bacterium, serves as an ideal microbial platform for F-dependent biochemical reactions due to its extraordinary resistance to harsh and stressful operating conditions. SinFonia will exploit natural selection to enhance bioproduction through a smart strain engineering approach in which bacterial growth will be coupled to biofluorination. Our target compounds are a whole family of fluorinated polyesters with enhanced physicochemical and material properties, with uses as self-cleaning surfaces, low-surface-energy coatings, bio-based lubricants, membranes for fuel cells, and anti-fouling materials. The versatile P. putida strains engineered during the project can be easily adapted to synthesize other added-value fluorochemicals. Unlike chemical processes, the source of F in our system will be NaF, an inexpensive and safe salt, and sugars as the main carbon source. In-depth analysis of all the environmental and economic benefits of the new fluorination technology, and interactive communication of social benefits associated with target products, are essential components of SinFonia.
Wissenschaftliches Gebiet
- engineering and technologymechanical engineeringtribologylubrication
- natural sciencesbiological sciencesmicrobiologybacteriology
- engineering and technologymaterials engineeringcoating and films
- natural scienceschemical sciencesinorganic chemistryhalogens
- engineering and technologyenvironmental engineeringenergy and fuelsfuel cells
Schlüsselbegriffe
Programm/Programme
Thema/Themen
Aufforderung zur Vorschlagseinreichung
Andere Projekte für diesen Aufruf anzeigenUnterauftrag
H2020-NMBP-BIO-2018-two-stage
Finanzierungsplan
RIA - Research and Innovation actionKoordinator
2800 Kongens Lyngby
Dänemark