Refactoring monoterpenoid indole alkaloid production in microbial cell factories

Información del proyecto

MIAMi

Identificador del acuerdo de subvención: 814645

DOI 10.3030/814645

Proyecto cerrado

Fecha de la firma de la CE 12 Diciembre 2018

Fecha de inicio 1 Enero 2019

Fecha de finalización 30 Junio 2023

Financiado con arreglo a

• INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies – Biotechnology

Coste total € 6 648 301,25

Aportación de la UE € 6 648 301,25

Coordinado por

DANMARKS TEKNISKE UNIVERSITET
Denmark

Resultado final

Websites, patent fillings, videos etc. (17)

Periodic newsletters (M36)

Periodic newsletters

Periodic newsletters (M12)

Periodic newsletters

Candidate gene lists
Validated growth-coupled methyl-transferase system for 1 MIA compound
>1,000 refactored and prototyped MIA pathway designs

1000 refactored and prototyped MIA pathway designs

Complete and publish deep learning algorithm on MIA pathway optimization in yeast cell factories
Periodic newsletters (M24)

Periodic newsletters

Expression atlas
Annotated reference genome of Rauvolfia tetraphylla
Periodic newsletters (M48)

Periodic newsletters

Project website: the report will provide a functional description of the project website and its main sections.
Technology Transfer (T&T) Activities, including training to SMEs and research centers

Technology Transfer TT Activities including training to SMEs and research centers

Press release about the launching of MIAMi project will be sent to the media to disseminate the project
Data processing pipeline for omics data
Publicly available DNA Parts repository
Publicly available Bio-CAD

Publicly available BioCAD

"Final version of the ""Plan for the Use and Dissemination of the Foreground"" (PUDF)"

Final version of the Plan for the Use and Dissemination of the Foreground PUDF

Documents, reports (5)

Life-Cycle and Techno-Economic Assessment - Final report

LifeCycle and TechnoEconomic Assessment Final report

Establish localization patterns of alkaloids in Rauvolfia sp.

Establish localization patterns of alkaloids in Rauvolfia sp

List of identified metabolites (and corresponding accurate masses) in Rauvolfia serpentina and Rauvolfia tetraphylla plants
Publicly available SOPs
Sample preparation methods for MSI studies of various parts of the Rauvolfia serpentina and Rauvolfia tetraphylla plants

Demonstrators, pilots, prototypes (1)

Delivery of large quantities of tabersonine, alstonine and rauwolscine

Delivery of large quantities of tabersonine alstonine and rauwolscine

Open Research Data Pilot (1)

First version of data management plan as required by Open Research Data Pilot

Publicaciones

Other (9)

Leaves of Cannabis sativa and their trichomes studied by DESI and MALDI mass spectrometry imaging for their contents of cannabinoids and flavonoids

Autores: Marcus Daniel Brandbjerg Bohn Lorensen, Sidra Yasmine Hayat, Niels Wellner, Nanna Bjarnholt, Christian Janfelt
Publicado en: 2023
Editor: NIH
DOI: 10.1002/pca.3202

Literate programming for iterative design-build-test-learn cycles in bioengineering

Autores: Søren Petersen, Lucas Levassor, Christine M. Pedersen, Rasmus Frandsen, Tilmann Weber, Jan Madsen, Lea G. Hansen, Jie Zhang, Ahmad K. Haidar, Jay D. Keasling, Nikolaus Sonnenchein, and Michael K. Jensen
Publicado en: 2023
Editor: BioRxiv
DOI: 10.1101/2023.06.18.545451

Directed Biosynthesis of New to Nature Alkaloids in a Heterologous Nicotiana benthamiana Expression Host

Autores: Marianna Boccia, Dagny Grzech, Adriana A. Lopes, Sarah E. O’Connor and Lorenzo Caputi
Publicado en: Frontiers, 2022
Editor: Frontiers in Plant Science
DOI: 10.3389/fpls.2022.919443

Boosting lignan-precursor synthesis in yeast cell factories through co-factor supply optimization

Autores: Jennifer Perrin, Sébastien Besseau, Nicolas Papon and Vincent Courdavault1
Publicado en: Frontiers, 2022
Editor: Frontiers
DOI: 10.3389/fbioe.2022.1079801

Spatial localization of monoterpenoid indole alkaloids in Rauvolfia tetraphylla by high resolution mass spectrometry imaging

Autores: Marcus Daniel Brandbjerg Bohn Lorensen, Nanna Bjarnholt, Benoit St-Pierre, Sarah Heinicke , Vincent Courdavault, Sarah O'Connor, Christian Janfelt
Publicado en: Phytochemistry, 2023
Editor: Phytochemistry
DOI: 10.1016/j.phytochem.2023.113620

Gaining access to acetyl-CoA by peroxisomal surface display

Autores: Thomas Perrot, Sébastien Besseau, Nicolas Papon, Vincent Courdavault
Publicado en: 2023
Editor: Synthetic and Systems Biotechnology
DOI: 10.1016/j.synbio.2023.02.003

Chromosome-scale genomes throw light on plant drug biosynthesis

Autores: Emily Amor Stander, Thomas Dugé de Bernonville, Nicolas Papon, Vincent Courdavault
Publicado en: Trends in Pharmacological Sciences, 2022
Editor: Trends in Pharmacological Sciences
DOI: 10.1016/j.tips.2022.01.007

HSP70-binding motifs function as protein quality control degrons

Autores: Amanda B. Abildgaard, Vasileios Voutsinos, Søren D. Petersen, Fia B. Larsen, Caroline Kampmeyer, Kristoffer E. Johansson, Amelie Stein, Tommer Ravid, Claes Andréasson, Michael K. Jensen, Kresten Lindorff-Larsen & Rasmus Hartmann-Petersen
Publicado en: 2023
Editor: Cellular and Molecular Life Sciences
DOI: 10.1007/s00018-022-04679-3

Early and Late Steps of Quinine Biosynthesis

Autores: Francesco Trenti, Kotaro Yamamoto, Benke Hong, Christian Paetz, Yoko Nakamura, and Sarah E. O’Connor
Publicado en: ACS Publications, 2021
Editor: ACS Publications
DOI: 10.1021/acs.orglett.1c00206

Peer reviewed articles (23)

Referee report. For: An updated version of the Madagascar periwinkle genome [version 1; peer review: 1 approved]

Autores: Tatsis, Evangelos
Publicado en: F1000Researcg, Edición 7, 2023, ISSN 2046-1402
Editor: F1000 Research Ltd.
DOI: 10.5256/f1000research.141880.r158640

Peroxisomes: A New Hub for Metabolic Engineering in Yeast

Autores: Natalja Kulagina, Sébastien Besseau, Nicolas Papon and Vincent Courdavault
Publicado en: Frontiers in Bioengineering and Biotechnology, 2021, ISSN 2296-4185
Editor: Frontiers in Bioengineering and Biotechnology
DOI: 10.3389/fbioe.2021.659431

Genome Assembly of the Medicinal Plant <i>Voacanga thouarsii</i>

Autores: Clément Cuello; Emily Amor Stander; Hans J Jansen; Thomas Dugé de Bernonville; Arnaud Lanoue; Nathalie Giglioli-Guivarc'h; Nicolas Papon; Ron P Dirks; Michael Krogh Jensen; Sarah Ellen O'Connor; Sébastien Besseau; Vincent Courdavault
Publicado en: Genome Biology and Evolution, Edición 3, 2022, ISSN 1759-6653
Editor: Oxford University Press
DOI: 10.1093/gbe/evac158

Tracking the Origin and Evolution of Plant Metabolites

Autores: Christophe Duplais, Nicolas Papon, Vincent Courdavault
Publicado en: Trends in plant science, 2020, Página(s) 1182-1184, ISSN 1878-4372
Editor: Trends in plant science
DOI: 10.1016/j.tplants.2020.08.010

Deploying Microbial Synthesis for Halogenating and Diversifying Medicinal Alkaloid Scaffolds

Autores: Samuel Alan Bradley; Jie Zhang; Michael Krogh Jensen
Publicado en: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020), Edición 2, 2020, ISSN 2296-4185
Editor: Frontiers in Bioengineering and Biotechnology
DOI: 10.3389/fbioe.2020.594126

A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae

Autores: Vasil D'Ambrosio, Lea G Hansen, Jie Zhang, Emil D Jensen, Dushica Arsovska, Marcos Laloux, Tadas Jakočiūnas, Pernille Hjort, Davide De Lucrezia , Serena Marletta, Jay D Keasling and Michael K Jensen
Publicado en: Synth Syst Biotechnol ., Edición 16;7(2), 2022, Página(s) 657-663, ISSN 2405-805X
Editor: KeAi Publishing Communications Ltd.
DOI: 10.1016/j.synbio.2022.02.001

Improved virus-induced gene silencing allows discovery of a serpentine synthase gene in Catharanthus roseus

Autores: Kotaro Yamamoto; Dagny Grzech; Konstantinos Koudounas; Emily Amor Stander; Lorenzo Caputi; Tetsuro Mimura; Vincent Courdavault; Sarah E. O’Connor
Publicado en: Plant Physiology, Edición 5, 2021, ISSN 0032-0889
Editor: American Society of Plant Biologists
DOI: 10.1093/plphys/kiab285

Computational biotechnology guides elucidation of the biosynthesis of the plant anticancer drug camptothecin

Autores: Emily Amor Stander, ThomasDugé de Bernonville, Nicolas Papon, Vincent Courdavault
Publicado en: Computational and Structural Biotechnology Journal, 2021, ISSN 2001-0370
Editor: Computational and Structural Biotechnology Journal
DOI: 10.1016/j.csbj.2021.06.028

Metabolic engineering for plant natural products biosynthesis: new procedures, concrete achievements and remaining limits

Autores: Nicolas Papon; Vincent Courdavault; Sarah O'Connor; Michael Krogh Jensen
Publicado en: Natural Product Reports, 38(12), Edición 5, 2021, Página(s) 2145-2153, ISSN 0265-0568
Editor: Royal Society of Chemistry
DOI: 10.1039/d0np00092b

Enhanced bioproduction of anticancer precursor vindoline by yeast cell factories

Autores: Natalja Kulagina, Grégory Guirimand, Céline Melin, Pamela Lemos-Cruz, Ines Carqueijeiro, Johan-Owen De Craene, Audrey Oudin, Vladimir Heredia, Konstantinos Koudounas, Marianne Unlubayir, Arnaud Lanoue, Nadine Imbault, Benoit St-Pierre, Nicolas Papon, Marc Clastre, Nathalie Giglioli-Guivarc’h, Jillian Marc, Sébastien Besseau, Vincent Courdavault
Publicado en: Microbial Biotechnology, 2021, Página(s) 2693-2699, ISSN 1751-7915
Editor: Microbial Biotechnology
DOI: 10.1111/1751-7915.13898

The Vinca minor genome highlights conserved evolutionary traits in monoterpene indole alkaloid synthesis.

Autores: Emily Amor Stander; Clément Cuello; Caroline Birer-Williams; Natalja Kulagina; Hans J Jansen; Ines Carqueijeiro; Louis-Valentin Méteignier; Valentin Vergès; Audrey Oudin; Nicolas Papon; Ron P Dirks; Michael Krogh Jensen; Sarah Ellen O’Connor; Thomas Dugé de Bernonville; Sébastien Besseau; Vincent Courdavault
Publicado en: G3: Genes, Genomes, Genetics, Edición 5, 2023, ISSN 2160-1836
Editor: Genetics Society of America
DOI: 10.1093/g3journal/jkac268

Supplying plant natural products by yeast cell factories

Autores: David Romero-Suarez, Jay D.Keasling and Michael K.Jensen1
Publicado en: Current Opinion in Green and Sustainable Chemistry, 2022, ISSN 2452-2236
Editor: Elsevier BV
DOI: 10.1016/j.cogsc.2021.100567

Efficient Terpene Production by Marine Thraustochytrids: Shedding Light on the Thermodynamic Driving Force

Autores: Natalja Kulagina, Jennifer Perrin, Sébastien Besseau, Vincent Courdavault
Publicado en: mBio, 2021, ISSN 2150-7511
Editor: mBio
DOI: 10.1128/mbio.01976-21

Identifying Missing Biosynthesis Enzymes of Plant Natural Products

Autores: Thomas Dugé de Bernonville, Nicolas Papon, Marc Clastre, Sarah E. O’Connor, Vincent Courdavault
Publicado en: Trends in Pharmacological Sciences, 2020, ISSN 0165-6147
Editor: Elsevier BV
DOI: 10.1016/j.tips.2019.12.006

Evolution-guided engineering of small-molecule biosensors

Autores: Tim Snoek, Evan K Chaberski, Francesca Ambri, Stefan Kol, Sara P Bjørn, Bo Pang, Jesus F Barajas, Ditte H Welner, Michael K Jensen, Jay D Keasling
Publicado en: Nucleic Acids Research, Edición 48/1, 2019, Página(s) e3-e3, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkz954

Beyond the semi-synthetic artemisinin: metabolic engineering of plant-derived anti-cancer drugs

Autores: Ines Carqueijeiro, Chloe Langley, Dagny Grzech, Konstantinos Koudounas, Nicolas Papon, Sarah E O’Connor, Vincent Courdavault
Publicado en: Current Opinion in Biotechnology, Edición 65, 2020, Página(s) 17-24, ISSN 0958-1669
Editor: Elsevier BV
DOI: 10.1016/j.copbio.2019.11.017

Towards the Microbial Production of Plant-Derived Anticancer Drugs

Autores: Vincent Courdavault, Sarah E. O’Connor, Audrey Oudin, Sébastien Besseau, Nicolas Papon
Publicado en: Trends in Cancer, Edición 6/6, 2020, Página(s) 444-448, ISSN 2405-8033
Editor: Elsevier
DOI: 10.1016/j.trecan.2020.02.004

Virus-Induced Gene Silencing: Hush Genes to Make Them Talk

Autores: Vincent Courdavault, Sébastien Besseau, Audrey Oudin, Nicolas Papon, Sarah Ellen O’Connor
Publicado en: Trends in Plant Science, Edición 25/7, 2020, Página(s) 714-715, ISSN 1360-1385
Editor: Elsevier BV
DOI: 10.1016/j.tplants.2020.02.013

Engineering G protein-coupled receptor signalling in yeast for biotechnological and medical purposes

Autores: Bettina Lengger, Michael K Jensen
Publicado en: FEMS Yeast Research, Edición 20/1, 2020, ISSN 1567-1356
Editor: Blackwell
DOI: 10.1093/femsyr/foz087

Identifying Genes Involved in alkaloid Biosynthesis in Vinca minor Through Transcriptomics and Gene Co-Expression Analysis

Autores: Emily A. Stander; Liuda Johana Sepúlveda; Thomas Dugé de Bernonville; Inês Carqueijeiro; Konstantinos Koudounas; Pamela Lemos Cruz; Sébastien Besseau; Arnaud Lanoue; Nicolas Papon; Nathalie Giglioli-Guivarc’h; Ron Dirks; Sarah E. O'Connor; Lucía Atehortúa; Audrey Oudin; Vincent Courdavault
Publicado en: Biomolecules, Edición 1, 2020, ISSN 2218-273X
Editor: MDPI
DOI: 10.3390/biom10121595

Yeasts as Biopharmaceutical Production Platforms

Autores: Natalja Kulagina, Sébastien Besseau, Charlotte Godon, Gustavo H. Goldman, Nicolas Papon and Vincent Courdavault
Publicado en: Frontiers in fungal biotechnology, 2021, ISSN 2054-3085
Editor: Frontiers in fungal biotechnology
DOI: 10.3389/ffunb.2021.733492

A microbial supply chain for production of the anti-cancer drug vinblastine

Autores: Jie Zhang; Lea G. Hansen; Olga Gudich; Konrad Viehrig; Lærke M. M. Lassen; Lars Schrübbers; Khem B. Adhikari; Paulina Rubaszka; Elena Carrasquer-Alvarez; Ling Chen; Vasil D’Ambrosio; Beata Lehka; Ahmad K. Haidar; Saranya Nallapareddy; Konstantina Giannakou; Marcos Laloux; Dushica Arsovska; Marcus A. K. Jørgensen; Leanne Jade G. Chan; Mette Kristensen; Hanne B. Christensen; Suresh Sudarsan; Em
Publicado en: Zhang , J , Hansen , L G , Gudich , O , Viehrig , K , Lassen , L M M , Schrübbers , L , Adhikari , K B , Rubaszka , P , Carrasquer-Alvarez , E , Chen , L , D’Ambrosio , V , Lehka , B , Haidar , A K , Nallapareddy , S , Giannakou , K , Laloux , M , Arsovska , D , Jørgensen , M A K , Chan , L J G , Kristensen , M , Christensen , H B , Sudarsan , S , Stander , E A , Baidoo , E , Petzold , C J , W, Edición 19, 2022, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/s41586-022-05157-3

Dietary Change Enables Robust Growth-Coupling of Heterologous Methyltransferase Activity in Yeast

Autores: Anne Sofie Lærke Hansen, Maitreya J. Dunham, Dushica Arsovska, Jie Zhang, Jay D. Keasling, Markus J. Herrgard, and Michael K. Jensen*
Publicado en: ACS Synthetic Biology, 2020, Página(s) 3408–3415, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.0c00348