Project description
Imaging of chromatin duplication at high resolution
DNA replication is a tightly regulated process in order to prevent errors in the control mechanisms linked to the development of cellular abnormalities and genetic diseases, including the onset of cancer. Recent studies revealed distinct steps leading to the initiation of eukaryotic genome duplication. Structural investigations so far have involved the imaging of isolated replication steps using simplified templates of linear duplex DNA. However, the natural substrate of the eukaryotic replication machinery is a compact chromatin. The EU-funded CRYOREP project will apply newly developed protocols to perform biochemistry experiments in combination with cryo-electron microscopy with the aim of visualising chromatin duplication at high resolution.
Fields of science
Programme(s)
Funding Scheme
ERC-COG - Consolidator Grant
Host institution
NW1 1AT London
United Kingdom
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Beneficiaries (1)
NW1 1AT London
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