Chromosome Replication Visualised by Cryo-EM

Project Information

CRYOREP

Grant agreement ID: 820102

DOI 10.3030/820102

EC signature date 17 December 2018

Start date 1 March 2019

End date 31 August 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 000 000,00

EU contribution € 2 000 000,00

Coordinated by

THE FRANCIS CRICK INSTITUTE LIMITED
United Kingdom

Publications

Peer reviewed articles (8)

Caught in the act: structural dynamics of replication origin activation and fork progression

Author(s): Jacob S. Lewis, Alessandro Costa
Published in: Biochemical Society Transactions, Issue 48/3, 2020, Page(s) 1057-1066, ISSN 0300-5127
Publisher: Portland Press, Ltd.
DOI: 10.1042/bst20190998

Microtubule Nucleation Properties of Single Human γTuRCs Explained by Their Cryo-EM Structure

Author(s): Tanja Consolati; Julia Locke; Johanna Roostalu; Zhuo Angel Chen; Julian Gannon; Jayant Asthana; Wei Ming Lim; Fabrizio Martino; Milos A. Cvetkovic; Juri Rappsilber; Alessandro Costa; Thomas Surrey
Published in: Developmental Cell, 2020, ISSN 1534-5807
Publisher: Cell Press
DOI: 10.1016/j.devcel.2020.04.019

Mechanism of replication origin melting nucleated by CMG helicase assembly

Author(s): Jacob S. Lewis, Marta H. Gross, Joana Sousa, Sarah S. Henrikus, Julia F. Greiwe, Andrea Nans, John F. X. Diffley & Alessandro Costa
Published in: Nature, 2022, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-022-04829-4

A Structure-Based Mechanism for DNA Entry into the Cohesin Ring

Author(s): Torahiko L. Higashi, Patrik Eickhoff, Joana S. Sousa, Julia Locke, Andrea Nans, Helen R. Flynn, Ambrosius P. Snijders, George Papageorgiou, Nicola O’Reilly, Zhuo A. Chen, Francis J. O’Reilly, Juri Rappsilber, Alessandro Costa, Frank Uhlmann
Published in: Molecular Cell, Issue 79/6, 2020, Page(s) 917-933.e9, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2020.07.013

Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer

Author(s): Valerie E Pye; David Rueda; Peter Cherepanov; Marcus Wilson; Mohamed Ghoneim; Ludovic Renault
Published in: Wilson , M , Renault , L , Maskell , D P , Ghoneim , M , Pye , V E , Nans , A , Rueda , D S , Cherepanov , P & Costa , A 2019 , ' Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer ' , Nature Communications , vol. 10 , 4189 . https://doi.org/10.1038/s41467-019-12007-w, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1101/663336

Mechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM

Author(s): Thomas C. R. Miller, Julia Locke, Julia F. Greiwe, John F. X. Diffley, Alessandro Costa
Published in: Nature, Issue 575/7784, 2019, Page(s) 704-710, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-019-1768-0

Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome

Author(s): Patrik Eickhoff, Hazal B. Kose, Fabrizio Martino, Tatjana Petojevic, Ferdos Abid Ali, Julia Locke, Nele Tamberg, Andrea Nans, James M. Berger, Michael R. Botchan, Hasan Yardimci, Alessandro Costa
Published in: Cell Reports, Issue 28/10, 2019, Page(s) 2673-2688.e8, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2019.07.104

Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase

Author(s): Julia F. Greiwe, Thomas C. R. Miller, Julia Locke, Fabrizio Martino, Steven Howell, Anne Schreiber, Andrea Nans, John F. X. Diffley & Alessandro Costa
Published in: Nature Structural & Molecular Biology, 2022, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-021-00698-z