Chromosome Replication Visualised by Cryo-EM

Project Information

CRYOREP

Grant agreement ID: 820102

DOI

10.3030/820102

Project closed

EC signature date 17 December 2018

Start date 1 March 2019

End date 31 August 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 000 000,00

EU contribution

€ 2 000 000,00

Coordinated by

THE FRANCIS CRICK INSTITUTE LIMITED
United Kingdom

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (17)

Caught in the act: structural dynamics of replication origin activation and fork progression

Author(s): Jacob S. Lewis, Alessandro Costa
Published in: Biochemical Society Transactions, Issue 48/3, 2020, Page(s) 1057-1066, ISSN 0300-5127
Publisher: Portland Press, Ltd.
DOI: 10.1042/bst20190998

MCM double hexamer loading visualized with human proteins

Author(s): Florian Weissmann, Julia F. Greiwe, Thomas Pühringer, Evelyn L. Eastwood, Emma C. Couves, Thomas C. R. Miller, John F. X. Diffley, Alessandro Costa
Published in: Nature, Issue 636, 2024, Page(s) 499-508, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-024-08263-6

Unidirectional MCM translocation away from ORC drives origin licensing

Author(s): Agata Butryn, Julia F. Greiwe, Alessandro Costa
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-025-56143-y

Microtubule Nucleation Properties of Single Human γTuRCs Explained by Their Cryo-EM Structure

Author(s): Tanja Consolati; Julia Locke; Johanna Roostalu; Zhuo Angel Chen; Julian Gannon; Jayant Asthana; Wei Ming Lim; Fabrizio Martino; Milos A. Cvetkovic; Juri Rappsilber; Alessandro Costa; Thomas Surrey
Published in: Developmental Cell, 2020, ISSN 1534-5807
Publisher: Cell Press
DOI: 10.1016/j.devcel.2020.04.019

In silico reconstitution of DNA replication. Lessons from single-molecule imaging and cryo-tomography applied to single-particle cryo-EM

Author(s): Julia F. Greiwe, Giulia Zanetti, Thomas C.R. Miller, Alessandro Costa
Published in: Current Opinion in Structural Biology, Issue 72, 2024, Page(s) 279-286, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2021.11.015

Unwinding of a eukaryotic origin of replication visualized by cryo-EM

Author(s): Sarah S. Henrikus, Marta H. Gross, Oliver Willhoft, Thomas Pühringer, Jacob S. Lewis, Allison W. McClure, Julia F. Greiwe, Giacomo Palm, Andrea Nans, John F. X. Diffley, Alessandro Costa
Published in: Nature Structural & Molecular Biology, Issue 31, 2024, Page(s) 1265-1276, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-024-01280-z

Mechanism of replication origin melting nucleated by CMG helicase assembly

Author(s): Jacob S. Lewis, Marta H. Gross, Joana Sousa, Sarah S. Henrikus, Julia F. Greiwe, Andrea Nans, John F. X. Diffley & Alessandro Costa
Published in: Nature, 2022, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-022-04829-4

A structural framework for DNA replication and transcription through chromatin

Author(s): Oliver Willhoft, Alessandro Costa
Published in: Current Opinion in Structural Biology, Issue 71, 2023, Page(s) 51-58, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2021.05.008

A Structure-Based Mechanism for DNA Entry into the Cohesin Ring

Author(s): Torahiko L. Higashi, Patrik Eickhoff, Joana S. Sousa, Julia Locke, Andrea Nans, Helen R. Flynn, Ambrosius P. Snijders, George Papageorgiou, Nicola O’Reilly, Zhuo A. Chen, Francis J. O’Reilly, Juri Rappsilber, Alessandro Costa, Frank Uhlmann
Published in: Molecular Cell, Issue 79/6, 2020, Page(s) 917-933.e9, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2020.07.013

Towards a Structural Mechanism for Sister Chromatid Cohesion Establishment at the Eukaryotic Replication Fork

Author(s): Sarah S. Henrikus, Alessandro Costa
Published in: Biology, Issue 10, 2025, Page(s) 466, ISSN 2079-7737
Publisher: MDPI AG
DOI: 10.3390/biology10060466

Multiple roles of Pol epsilon in eukaryotic chromosome replication

Author(s): Milos A. Cvetkovic, Esther Ortega, Roberto Bellelli, Alessandro Costa
Published in: Biochemical Society Transactions, Issue 50, 2022, Page(s) 309-320, ISSN 0300-5127
Publisher: Portland Press, Ltd.
DOI: 10.1042/bst20210082

Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer

Author(s): Valerie E Pye; David Rueda; Peter Cherepanov; Marcus Wilson; Mohamed Ghoneim; Ludovic Renault
Published in: Nature Communications, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1101/663336

Mechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM

Author(s): Thomas C. R. Miller, Julia Locke, Julia F. Greiwe, John F. X. Diffley, Alessandro Costa
Published in: Nature, Issue 575/7784, 2019, Page(s) 704-710, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-019-1768-0

The structural mechanism of dimeric DONSON in replicative helicase activation

Author(s): Milos A. Cvetkovic, Paolo Passaretti, Agata Butryn, Alicja Reynolds-Winczura, Georgia Kingsley, Aggeliki Skagia, Cyntia Fernandez-Cuesta, Divyasree Poovathumkadavil, Roger George, Anoop S. Chauhan, Satpal S. Jhujh, Grant S. Stewart, Agnieszka Gambus, Alessandro Costa
Published in: Molecular Cell, Issue 83, 2023, Page(s) 4017-4031.e9, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2023.09.029

Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome

Author(s): Patrik Eickhoff, Hazal B. Kose, Fabrizio Martino, Tatjana Petojevic, Ferdos Abid Ali, Julia Locke, Nele Tamberg, Andrea Nans, James M. Berger, Michael R. Botchan, Hasan Yardimci, Alessandro Costa
Published in: Cell Reports, Issue 28/10, 2019, Page(s) 2673-2688.e8, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2019.07.104

Protocol for the purification of replisomes from the Xenopus laevis egg extract system for single-particle cryo-EM analysis

Author(s): Paolo Passaretti, Milos A. Cvetkovic, Alessandro Costa, Agnieszka Gambus
Published in: STAR Protocols, Issue 5, 2025, Page(s) 103237, ISSN 2666-1667
Publisher: Elsevier BV
DOI: 10.1016/j.xpro.2024.103237

Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase

Author(s): Julia F. Greiwe, Thomas C. R. Miller, Julia Locke, Fabrizio Martino, Steven Howell, Anne Schreiber, Andrea Nans, John F. X. Diffley & Alessandro Costa
Published in: Nature Structural & Molecular Biology, 2022, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-021-00698-z