During the EthnoHERBS project, a comprehensive report on the classification of collected data was completed. Information on traditional herbal medicines used to treat various skin disorders was gathered from:
(I) scientific publications on ethnobotanical surveys, (II) ancient and Byzantine texts and
(III) manuscript collections.
From this research, 209 plant species from 58 families were collected in their natural habitats across the Balkan Peninsula and deposited in herbaria. In silico screening against skin disorder-related targets led to the identification of specific plant-target interactions of pharmacological interest.
To optimize bioactive compound recovery and mirror traditional preparation methods, a total of 739 extracts were prepared using environmentally friendly protocols tailored to each plant’s properties.
The extracts underwent advanced analysis using spectroscopic and chromatographic techniques, resulting in the identification of over 500 compounds. These extracts were then assessed for antioxidant activity, total phenolic and flavonoid content, and inhibitory effects on enzymes linked to aging, inflammation, and pigmentation disorders. The results validated their traditional medicinal uses.
Based on bioactivity profiles, 24 extracts from 16 promising species were selected for further evaluation. A categorized list of the most effective extracts was compiled, targeting specific dermatological applications: anti-aging, wound healing, anti-melanoma, and anti-inflammatory.
In parallel, an in silico toxicity assessment was performed on 120 compounds from the 24 selected extracts, focusing on potential skin irritation and sensitization. Most compounds exhibited favorable safety profiles and low mutagenic potential.
Sustainability was a parallel focus. 25 plants were evaluated in short-term pre-cultivation trials to determine optimal propagation. 9 species with strong adaptability were selected and cultivated in the field. For pilot-scale development, selected species underwent scaled extraction followed by stability and microbiological testing. Cistus leaves, notable for both abundance and activity, were used to produce two pilot-scale extracts. These were formulated into 3 cosmetic products translating research results into practical outputs.
Management, Entrepreneurship, and Training
Secondees training covered IP protection, commercialisation strategies, IP portfolio management, innovation processes, licensing, spin-off creation, and negotiation techniques. It aimed to accelerate the adoption of best practices in TT and innovation management. Additionally, Regulatory Affairs Training was provided, offering detailed guidance on EU regulations concerning botanical raw materials, personal care products, cosmeceuticals, and herbal pharmaceuticals. This enabled secondees to better navigate product development, ensuring regulatory compliance and smoother market access.
From the outset, communication and dissemination were key priorities. Most partners actively presented project goals and outcomes at scientific events and international exhibitions. Articles and newsletters were published in both academic journals and broader media outlets. A public website was launched early and served as a central hub for updates, reports, and news. A distinctive project logo was used across all materials. Over the course of the project, 3 international workshops and 2 webinars were held, promoting collaboration and knowledge sharing.
To date, 23 open-access scientific papers have been published, with additional manuscripts under development. EthnoHERBS gained significant attention across Europe and internationally, with outreach activities in various languages. Its dual mission—to conserve European biodiversity and revive traditional herbal knowledge—has been widely recognized in academic, professional, and public spheres.
Project coordination was smooth and effective throughout. All management and administrative responsibilities were fulfilled, ensuring strong communication between beneficiaries and with the European Commission. Regular meetings and consistent reporting supported steady progress. No issues were reported in collaboration among partners or with the coordinator.
