Connecting neural networks: Nervous-system-on-Chip Technology

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Chip compatible culture protocol for functional midbrain/hindbrain generation
Finalizing the activity and transport measurement in multiple compartment iPSC derived cultures
Demonstration of electrode integration with silicon sieve device
Electrophysiological characterization of control and patient-derived midbrain organoids.
Array device demonstration
Characterization of network activity by fluorescence-based calcium imaging.
Characterization of iPSC derived ENS networks using Ca2+ and axonal transport imaging.
Individual electrode chemical and electrochemical characterization
Floating organoids for experiments at the partners’ lab in ErasmusMC, USFD and KULeuven
Optimized protocol for the differentiation of hPSC-derived vagal NC toward enteric neurons
Neuron compatibility study of electrodes
Demonstration of integrated electrode array in a fluidic device
Specifications of preferred surface chemistry of an electrode for a chosen analyte
Microsieve MEA chip
Finalizing the explorative measurements concerning α-synuclein transport and laser-induced lesions in iPSC derived ENS
Evaluation of the utility of the intestinal organoid/ENS co-culture system and self-renewing vagal and trunk NC spheres.

Report on electrode scaling

Website CONNECT online
CONNECT Stakeholder meeting

Open Research Data

Publications

Synaptic decline precedes dopaminergic neuronal loss in human midbrain organoids harboring a triplication of the <i>SNCA</i> gene

Author(s): Jennifer Modamio, Claudia Saraiva, Gemma Gomez Giro, Sarah Louise Nickels, Javier Jarazo, Paul Antony, Peter Barbuti, Rashi Hadler, Christian Jäger, Rejko Krüger, Enrico Glaab, Jens Christian Schwamborn
Published in: 2021
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.07.15.452499

Retinoic acid accelerates the specification of enteric neural progenitors from<i>in vitro</i>-derived neural crest

Author(s): Thomas J.R Frith, Antigoni Gogolou, James O.S Hackland, Ivana Barbaric, Nikhil Thapar, Alan J. Burns, Peter W Andrews, Anestis Tsakiridis, Conor J. McCann
Published in: 2022
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/819748

Human pluripotent stem cells: tools for regenerative medicine.

Author(s): Andrews, P.W.
Published in: Biomaterials translational, 2021
Publisher: Chinese Medical Multimedia Press
DOI: 10.12336/biomatertransl.2021.04.004

Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives

Author(s): Matthew Wind, Antigoni Gogolou, Ichcha Manipur, Ilaria Granata, Larissa Butler, Peter W. Andrews, Ivana Barbaric, Ke Ning, Mario R. Guarracino, Marysia Placzek, Anestis Tsakiridis
Published in: Development, Issue 148/6, 2021, ISSN 0950-1991
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/dev.194415

Microenvironments Matter: Advances in Brain-on-Chip

Author(s): Akcay, G.; Luttge, R.
Published in: Biosensors, 2023, ISSN 2079-6374
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/bios13050551

Midbrain Organoids: A New Tool to Investigate Parkinson’s Disease

Author(s): Lisa Maria Smits, Jens Christian Schwamborn
Published in: Frontiers in Cell and Developmental Biology, Issue 8, 2022, ISSN 2296-634X
Publisher: Frontiers in Cell and Developmental Biology
DOI: 10.3389/fcell.2020.00359

Local axonal morphology guides the topography of interneuron myelination in mouse and human neocortex

Author(s): Jeffrey Stedehouder, Demi Brizee, Johan A Slotman, Maria Pascual-Garcia, Megan L Leyrer, Bibi LJ Bouwen, Clemens MF Dirven, Zhenyu Gao, David M Berson, Adriaan B Houtsmuller, Steven A Kushner
Published in: eLife, Issue 8, 2023, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.48615

A robust protocol for the generation of human midbrain organoids

Author(s): Alise Zagare, Matthieu Gobin, Anna S. Monzel, Jens C. Schwamborn
Published in: STAR Protocols, Issue 2, 2022, Page(s) 100524, ISSN 2666-1667
Publisher: Elsevier
DOI: 10.1016/j.xpro.2021.100524

Rapid industrial scale synthesis of robust carbon nanotube network electrodes for electroanalysis

Author(s): Elli Leppänen, Jarkko Etula, Peter Engelhardt, Sami Sainio, Hua Jiang, Björn Mikladal, Antti Peltonen, Ilkka Varjos, Tomi Laurila
Published in: Journal of Electroanalytical Chemistry, Issue 896, 2022, Page(s) 115255, ISSN 1572-6657
Publisher: Elsevier BV
DOI: 10.1016/j.jelechem.2021.115255

Nanogrooves for 2D and 3D Microenvironments of SH-SY5Y Cultures in Brain-on-Chip Technology

Author(s): Alex Bastiaens, Rahman Sabahi-Kaviani, Regina Luttge
Published in: Frontiers in Neuroscience, Issue 14, 2020, ISSN 1662-453X
Publisher: Frontiers Media SA
DOI: 10.3389/fnins.2020.00666

In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells

Author(s): Matt Wind, Anestis Tsakiridis
Published in: Current Protocols, Issue 1, 2023, ISSN 2691-1299
Publisher: John Wiley & Sons
DOI: 10.1002/cpz1.244

Analyzing Developing Brain-On-Chip Cultures with the CALIMA Calcium Imaging Tool

Author(s): Elles A. L. Raaijmakers, Nikki Wanders, Rob M. C. Mestrom, Regina Luttge
Published in: Micromachines, Issue 12/4, 2021, Page(s) 412, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12040412

Gut innervation and enteric nervous system development: a spatial, temporal and molecular tour de force

Author(s): Yi-Ning Kang, Candice Fung, Pieter Vanden Berghe
Published in: Development, Issue 148/3, 2021, ISSN 0950-1991
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/dev.182543

Real-time selective detection of dopamine and serotonin at nanomolar concentration from complex in vitro systems

Author(s): Samuel Rantataro, Ilmari Parkkinen, Mikko Airavaara, Tomi Laurila
Published in: Biosensors and Bioelectronics, Issue 241, 2023, Page(s) 115579, ISSN 0956-5663
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2023.115579

Nanofabricating neural networks: Strategies, advances, and challenges

Author(s): Regina Luttge
Published in: Journal of Vacuum Science & Technology B, Issue 40, 2024, ISSN 2166-2754
Publisher: AVS Science and Technology Society
DOI: 10.1116/6.0001649

Reproducible generation of human midbrain organoids for in vitro modeling of Parkinson’s disease

Author(s): Sarah Louise Nickels, Jennifer Modamio, Bárbara Mendes-Pinheiro, Anna Sophia Monzel, Fay Betsou, Jens Christian Schwamborn
Published in: Stem Cell Research, Issue 46, 2020, Page(s) 101870, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2020.101870

Shape Memory Polymer-Based Insertable Electrode Array Towards Minimally Invasive Subdural Implantation

Author(s): Anastasiia Kravtcova, Antoniya Toncheva, Samuel Rantataro, Emilia Peltola, Jean-Marie Raquez, Pierre Lambert, Quan Zhou
Published in: IEEE Sensors Journal, Issue 21, 2021, Page(s) 17282-17289, ISSN 1530-437X
Publisher: Institute of Electrical and Electronics Engineers
DOI: 10.1109/jsen.2021.3078358

Trends in Carbon, Oxygen, and Nitrogen Core in the X-ray Absorption Spectroscopy of Carbon Nanomaterials: A Guide for the Perplexed

Author(s): Sami Sainio, Niklas Wester, Anja Aarva, Charles J. Titus, Dennis Nordlund, Esko I. Kauppinen, Elli Leppänen, Tommi Palomäki, Jessica E. Koehne, Olli Pitkänen, Krisztian Kordas, Maria Kim, Harri Lipsanen, Miran Mozetič, Miguel A. Caro, M. Meyyappan, Jari Koskinen, Tomi Laurila
Published in: The Journal of Physical Chemistry C, Issue 125/1, 2021, Page(s) 973-988, ISSN 1932-7447
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcc.0c08597

Integrated, automated maintenance, expansion and differentiation of 2D and 3D patient-derived cellular models for high throughput drug screening

Author(s): Ibrahim Boussaad, Gérald Cruciani, Silvia Bolognin, Paul Antony, Claire M. Dording, Yong-Jun Kwon, Peter Heutink, Eugenio Fava, Jens C. Schwamborn, Rejko Krüger
Published in: Scientific Reports, Issue 11/1, 2021, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-81129-3

Human enteric nervous system progenitor transplantation improves functional responses in Hirschsprung disease patient-derived tissue

Author(s): Benjamin Jevans, Fay Cooper, Yuliia Fatieieva, Antigoni Gogolou, Yi-Ning Kang, Restuadi Restuadi, Dale Moulding, Pieter Vanden Berghe, Igor Adameyko, Nikhil Thapar, Peter W Andrews, Paolo De Coppi, Anestis Tsakiridis, Conor J McCann
Published in: Gut, Issue 73, 2024, Page(s) 1441-1453, ISSN 0017-5749
Publisher: BMJ Publishing Group
DOI: 10.1136/gutjnl-2023-331532

Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells

Author(s): Antigoni Gogolou, Thomas J.R. Frith, Anestis Tsakiridis
Published in: Current Protocols, Issue 1/6, 2021, ISSN 2691-1299
Publisher: Wiley
DOI: 10.1002/cpz1.137

Nanostructured Geometries Strongly Affect Fouling of Carbon Electrodes

Author(s): Ayesha Kousar, Emilia Peltola, Tomi Laurila
Published in: ACS Omega, Issue 6, 2023, Page(s) 26391-26403, ISSN 2470-1343
Publisher: American Chemical Society
DOI: 10.1021/acsomega.1c03666

Ascorbic acid does not necessarily interfere with the electrochemical detection of dopamine

Author(s): Samuel Rantataro, Laura Ferrer Pascual, Tomi Laurila
Published in: Scientific Reports, Issue 12, 2022, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-022-24580-0

Notch signalling influences cell fate decisions and HOX gene induction in axial progenitors

Author(s): Fay Cooper, Celine Souilhol, Scott Haston, Shona Gray, Katy Boswell, Antigoni Gogolou, Thomas J. R. Frith, Dylan Stavish, Bethany M. James, Daniel Bose, Jacqueline Kim Dale, Anestis Tsakiridis
Published in: Development, Issue 151, 2024, ISSN 0950-1991
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/dev.202098

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Author(s): Rahman Sabahi-Kaviani, Regina Luttge
Published in: Micromachines, Issue 12, 2024, Page(s) 21, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Spontaneous Epileptic Recordings from hiPSC-Derived Cortical Neurons Cultured with a Human Epileptic Brain Biopsy on a Multi Electrode Array

Author(s): Michel H. Y. Hu, Jean-Philippe Frimat, Kim Rijkers, Olaf E. M. G. Schijns, Arn M. J. M. van den Maagdenberg, Jim T. A. Dings, Regina Luttge, Govert Hoogland
Published in: Applied Sciences, Issue 13, 2025, Page(s) 1432, ISSN 2076-3417
Publisher: MDPI Applied Science
DOI: 10.3390/app13031432

Three-Dimensional Fine Structure of Nanometer-Scale Nafion Thin Films

Author(s): A. Peltonen, J. Etula, J. Seitsonen, P. Engelhardt, T. Laurila
Published in: ACS Applied Polymer Materials, Issue 3/2, 2021, Page(s) 1078-1086, ISSN 2637-6105
Publisher: American Chemical Society
DOI: 10.1021/acsapm.0c01318

Electrochemical detection of amine neurotransmitters is drastically different in buffer solutions, in vivo, and cell culture systems

Author(s): Samuel Rantataro, Laura Ferrer Pascual, Tomi Laurila
Published in: Electrochemistry Communications, 2024, Page(s) 107732, ISSN 1388-2481
Publisher: Elsevier BV
DOI: 10.1016/j.elecom.2024.107732

Retinoic Acid Accelerates the Specification of Enteric Neural Progenitors from In-Vitro-Derived Neural Crest

Author(s): Thomas J.R. Frith, Antigoni Gogolou, James O.S. Hackland, Zoe A. Hewitt, Harry D. Moore, Ivana Barbaric, Nikhil Thapar, Alan J. Burns, Peter W. Andrews, Anestis Tsakiridis, Conor J. McCann
Published in: Stem Cell Reports, Issue 15, 2022, Page(s) 557-565, ISSN 2213-6711
Publisher: Cell Press
DOI: 10.1016/j.stemcr.2020.07.024

A short history of pluripotent stem cells markers

Author(s): Peter W. Andrews, Paul J. Gokhale
Published in: Stem Cell Reports, Issue 19, 2024, Page(s) 1-10, ISSN 2213-6711
Publisher: Cell Press
DOI: 10.1016/j.stemcr.2023.11.012

Stacked hydrogel-based brain-on-chips utilizing capillary force flow pinning

Author(s): Gulden Akcay, Jeroen van Venrooij, Regina Luttge
Published in: Journal of Vacuum Science & Technology B, Issue 42, 2024, ISSN 2166-2754
Publisher: AVS Science and Technology Society
DOI: 10.1116/6.0003967

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Author(s): Rahman Sabahi-Kaviani, Regina Luttge
Published in: Micromachines, Issue 12/1, 2021, Page(s) 21, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Electrical monitoring approaches in 3-dimensional cell culture systems: Toward label-free, high spatiotemporal resolution, and high-content data collection in vitro

Author(s): Yagmur Demircan Yalcin, Regina Luttge
Published in: Organs-on-a-Chip, Issue 3, 2021, Page(s) 100006, ISSN 2666-1020
Publisher: Elsevier
DOI: 10.1016/j.ooc.2021.100006

Nanogroove-Enhanced Hydrogel Scaffolds for 3D Neuronal Cell Culture: An Easy Access Brain-on-Chip Model

Author(s): Bastiaens, Xie, Luttge
Published in: Micromachines, Issue 10/10, 2019, Page(s) 638, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi10100638

Exploiting nanogroove-induced cell culture anisotropy to advance in vitro brain models

Author(s): Alex Bastiaens, Jean-Philippe Frimat, Teun van Nunen, Regina Luttge
Published in: Journal of Vacuum Science & Technology B, Issue 37/6, 2019, Page(s) 061802, ISSN 2166-2754
Publisher: AVS Science and Technology Society
DOI: 10.1116/1.5119687

Modeling early phenotypes of Parkinson’s disease by age-induced midbrain-striatum assembloids

Author(s): Kyriaki Barmpa, Claudia Saraiva, Diego Lopez-Pigozzi, Gemma Gomez-Giro, Elisa Gabassi, Sarah Spitz, Konstanze Brandauer, Juan E. Rodriguez Gatica, Paul Antony, Graham Robertson, Rahman Sabahi-Kaviani, Alessandro Bellapianta, Florentia Papastefanaki, Regina Luttge, Ulrich Kubitscheck, Ahmad Salti, Peter Ertl, Mario Bortolozzi, Rebecca Matsas, Frank Edenhofer, Jens C. Schwamborn
Published in: Communications Biology, Issue 7, 2024, ISSN 2399-3642
Publisher: Communications Biology
DOI: 10.1038/s42003-024-07273-4

Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells

Author(s): Fay Cooper, Anestis Tsakiridis
Published in: Biochemical Society Transactions, Issue 50, 2022, Page(s) 499-511, ISSN 0300-5127
Publisher: Portland Press, Ltd.
DOI: 10.1042/bst20211152

Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture

Author(s): Gulden Akcay, Regina Luttge
Published in: Micromachines, Issue 12/2, 2021, Page(s) 165, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12020165

X-ray Spectroscopy Fingerprints of Pristine and Functionalized Graphene

Author(s): Anja Aarva, Sami Sainio, Volker L. Deringer, Miguel A. Caro, Tomi Laurila
Published in: The Journal of Physical Chemistry C, Issue 125, 2023, Page(s) 18234-18246, ISSN 1932-7447
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcc.1c03238

A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations

Author(s): Ingrid M. Saldana-Guerrero, Luis F. Montano-Gutierrez, Katy Boswell, Christoph Hafemeister, Evon Poon, Lisa E. Shaw, Dylan Stavish, Rebecca A. Lea, Sara Wernig-Zorc, Eva Bozsaky, Irfete S. Fetahu, Peter Zoescher, Ulrike Pötschger, Marie Bernkopf, Andrea Wenninger-Weinzierl, Caterina Sturtzel, Celine Souilhol, Sophia Tarelli, Mohamed R. Shoeb, Polyxeni Bozatzi, Magdalena Rados, Maria Guarini, Mich
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-47945-7

Microfabrication atomic layer deposited Pt NPs/TiN thin film on silicon as a nanostructure signal Transducer: Electrochemical characterization toward neurotransmitter sensing

Author(s): Yalda Shoja, Noora Isoaho, Ville Jokinen, Sami Franssila
Published in: Applied Surface Science, Issue 573, 2024, Page(s) 151444, ISSN 0169-4332
Publisher: Elsevier BV
DOI: 10.1016/j.apsusc.2021.151444

Understanding axial progenitor biology in vivo and in vitro

Author(s): Filip J. Wymeersch, Valerie Wilson, Anestis Tsakiridis
Published in: Development, Issue 148/4, 2021, ISSN 0950-1991
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/dev.180612

The origins of human pluripotent stem cells: the road from a cancer to regenerative medicine

Author(s): Peter W. Andrews
Published in: In Vitro Cellular & Developmental Biology - Animal, Issue 60, 2024, Page(s) 514-520, ISSN 1071-2690
Publisher: Tissue Culture Association
DOI: 10.1007/s11626-024-00865-8

Computational study of mechanical stresses in a cell interacting with micromechanical cues and microfabrication of such cues in Nervous system-on-Chips

Author(s): Rahman Sabahi-Kaviani, Daan van Boekel, Regina Luttge
Published in: Journal of Vacuum Science & Technology B, Issue 41, 2023, ISSN 2166-2754
Publisher: AVS Science and Technology Society
DOI: 10.1116/6.0002291

Effect of etchant gases on the structure and properties of carbon nanofibers

Author(s): Ishan Pande, Laura Ferrer Pascual, Ayesha Kousar, Jani Sainio, Hua Jiang, Tomi Laurila
Published in: Diamond and Related Materials, Issue 144, 2024, Page(s) 111004, ISSN 0925-9635
Publisher: Elsevier BV
DOI: 10.1016/j.diamond.2024.111004

3D-electrode integrated microsieve structure as a rapid and cost-effective single neuron detector

Author(s): Yagmur Demircan Yalcin, Regina Luttge
Published in: Journal of Vacuum Science & Technology B, Issue 38/6, 2020, Page(s) 063202, ISSN 2166-2754
Publisher: AVS Science and Technology Society
DOI: 10.1116/6.0000518

Modulating the Geometry of the Carbon Nanofiber Electrodes Provides Control over Dopamine Sensor Performance

Author(s): Ayesha Kousar, Ishan Pande, Laura F. Pascual, Emilia Peltola, Jani Sainio, Tomi Laurila
Published in: Analytical Chemistry, Issue 95, 2023, Page(s) 2983-2991, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.2c04843

Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor

Author(s): Antigoni Gogolou, Celine Souilhol, Ilaria Granata, Filip J Wymeersch, Ichcha Manipur, Matthew Wind, Thomas JR Frith, Maria Guarini, Alessandro Bertero, Christoph Bock, Florian Halbritter, Minoru Takasato, Mario R Guarracino, Anestis Tsakiridis
Published in: eLife, Issue 11, 2023, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.74263

Editorial for the Special Issue on Microfluidic Brain-on-a-Chip

Author(s): Regina Luttge
Published in: Micromachines, Issue 12, 2025, Page(s) 1100, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12091100

Searching for OpenAIRE data...

Deliverables

Publications

Share this page Share this page on social networks

Download Download the content of the page