Connecting neural networks: Nervous-system-on-Chip Technology

Project Information

CONNECT

Grant agreement ID: 824070

DOI 10.3030/824070

Project closed

EC signature date 23 October 2018

Start date 1 January 2019

End date 30 June 2024

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost € 6 807 867,50

EU contribution € 6 807 867,50

Coordinated by

TECHNISCHE UNIVERSITEIT EINDHOVEN
Netherlands

Deliverables

Demonstrators, pilots, prototypes (16)

Chip compatible culture protocol for functional midbrain/hindbrain generation
Finalizing the activity and transport measurement in multiple compartment iPSC derived cultures
Demonstration of electrode integration with silicon sieve device
Electrophysiological characterization of control and patient-derived midbrain organoids.
Array device demonstration
Characterization of network activity by fluorescence-based calcium imaging.
Characterization of iPSC derived ENS networks using Ca2+ and axonal transport imaging.
Individual electrode chemical and electrochemical characterization
Floating organoids for experiments at the partners’ lab in ErasmusMC, USFD and KULeuven
Optimized protocol for the differentiation of hPSC-derived vagal NC toward enteric neurons
Neuron compatibility study of electrodes
Demonstration of integrated electrode array in a fluidic device
Specifications of preferred surface chemistry of an electrode for a chosen analyte
Microsieve MEA chip
Finalizing the explorative measurements concerning α-synuclein transport and laser-induced lesions in iPSC derived ENS
Evaluation of the utility of the intestinal organoid/ENS co-culture system and self-renewing vagal and trunk NC spheres.

Documents, reports (1)

Report on electrode scaling

Websites, patent fillings, videos etc. (1)

Website CONNECT online

Publications

Peer reviewed articles (16)

Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives

Author(s): Matthew Wind, Antigoni Gogolou, Ichcha Manipur, Ilaria Granata, Larissa Butler, Peter W. Andrews, Ivana Barbaric, Ke Ning, Mario R. Guarracino, Marysia Placzek, Anestis Tsakiridis
Published in: Development, Issue 148/6, 2021, ISSN 0950-1991
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/dev.194415

Nanogrooves for 2D and 3D Microenvironments of SH-SY5Y Cultures in Brain-on-Chip Technology

Author(s): Alex Bastiaens, Rahman Sabahi-Kaviani, Regina Luttge
Published in: Frontiers in Neuroscience, Issue 14, 2020, ISSN 1662-453X
Publisher: Frontiers Media SA
DOI: 10.3389/fnins.2020.00666

Analyzing Developing Brain-On-Chip Cultures with the CALIMA Calcium Imaging Tool

Author(s): Elles A. L. Raaijmakers, Nikki Wanders, Rob M. C. Mestrom, Regina Luttge
Published in: Micromachines, Issue 12/4, 2021, Page(s) 412, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12040412

Gut innervation and enteric nervous system development: a spatial, temporal and molecular tour de force

Author(s): Yi-Ning Kang, Candice Fung, Pieter Vanden Berghe
Published in: Development, Issue 148/3, 2021, ISSN 0950-1991
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/dev.182543

Reproducible generation of human midbrain organoids for in vitro modeling of Parkinson’s disease

Author(s): Sarah Louise Nickels, Jennifer Modamio, Bárbara Mendes-Pinheiro, Anna Sophia Monzel, Fay Betsou, Jens Christian Schwamborn
Published in: Stem Cell Research, Issue 46, 2020, Page(s) 101870, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2020.101870

Trends in Carbon, Oxygen, and Nitrogen Core in the X-ray Absorption Spectroscopy of Carbon Nanomaterials: A Guide for the Perplexed

Author(s): Sami Sainio, Niklas Wester, Anja Aarva, Charles J. Titus, Dennis Nordlund, Esko I. Kauppinen, Elli Leppänen, Tommi Palomäki, Jessica E. Koehne, Olli Pitkänen, Krisztian Kordas, Maria Kim, Harri Lipsanen, Miran Mozetič, Miguel A. Caro, M. Meyyappan, Jari Koskinen, Tomi Laurila
Published in: The Journal of Physical Chemistry C, Issue 125/1, 2021, Page(s) 973-988, ISSN 1932-7447
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcc.0c08597

Integrated, automated maintenance, expansion and differentiation of 2D and 3D patient-derived cellular models for high throughput drug screening

Author(s): Ibrahim Boussaad, Gérald Cruciani, Silvia Bolognin, Paul Antony, Claire M. Dording, Yong-Jun Kwon, Peter Heutink, Eugenio Fava, Jens C. Schwamborn, Rejko Krüger
Published in: Scientific Reports, Issue 11/1, 2021, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-81129-3

Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells

Author(s): Antigoni Gogolou, Thomas J.R. Frith, Anestis Tsakiridis
Published in: Current Protocols, Issue 1/6, 2021, ISSN 2691-1299
Publisher: Wiley
DOI: 10.1002/cpz1.137

Three-Dimensional Fine Structure of Nanometer-Scale Nafion Thin Films

Author(s): A. Peltonen, J. Etula, J. Seitsonen, P. Engelhardt, T. Laurila
Published in: ACS Applied Polymer Materials, Issue 3/2, 2021, Page(s) 1078-1086, ISSN 2637-6105
Publisher: American Chemical Society
DOI: 10.1021/acsapm.0c01318

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Author(s): Rahman Sabahi-Kaviani, Regina Luttge
Published in: Micromachines, Issue 12/1, 2021, Page(s) 21, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Electrical monitoring approaches in 3-dimensional cell culture systems: Toward label-free, high spatiotemporal resolution, and high-content data collection in vitro

Author(s): Yagmur Demircan Yalcin, Regina Luttge
Published in: Organs-on-a-Chip, Issue 3, 2021, Page(s) 100006, ISSN 2666-1020
Publisher: Elsevier
DOI: 10.1016/j.ooc.2021.100006

Nanogroove-Enhanced Hydrogel Scaffolds for 3D Neuronal Cell Culture: An Easy Access Brain-on-Chip Model

Author(s): Bastiaens, Xie, Luttge
Published in: Micromachines, Issue 10/10, 2019, Page(s) 638, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi10100638

Exploiting nanogroove-induced cell culture anisotropy to advance in vitro brain models

Author(s): Alex Bastiaens, Jean-Philippe Frimat, Teun van Nunen, Regina Luttge
Published in: Journal of Vacuum Science & Technology B, Issue 37/6, 2019, Page(s) 061802, ISSN 2166-2754
Publisher: AVS Science and Technology Society
DOI: 10.1116/1.5119687

Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture

Author(s): Gulden Akcay, Regina Luttge
Published in: Micromachines, Issue 12/2, 2021, Page(s) 165, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12020165

Understanding axial progenitor biology in vivo and in vitro

Author(s): Filip J. Wymeersch, Valerie Wilson, Anestis Tsakiridis
Published in: Development, Issue 148/4, 2021, ISSN 0950-1991
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/dev.180612

3D-electrode integrated microsieve structure as a rapid and cost-effective single neuron detector

Author(s): Yagmur Demircan Yalcin, Regina Luttge
Published in: Journal of Vacuum Science & Technology B, Issue 38/6, 2020, Page(s) 063202, ISSN 2166-2754
Publisher: AVS Science and Technology Society
DOI: 10.1116/6.0000518

Non-peer reviewed articles (1)

Microenvironments Matter: Advances in Brain-on-Chip

Author(s): Akcay, G.; Luttge, R.
Published in: Biosensors, 2023, ISSN 2079-6374
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/bios13050551