Connecting neural networks: Nervous-system-on-Chip Technology

Informations projet

CONNECT

N° de convention de subvention: 824070

DOI 10.3030/824070

Projet clôturé

Date de signature de la CE 23 Octobre 2018

Date de début 1 Janvier 2019

Date de fin 30 Juin 2024

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total € 6 807 867,50

Contribution de l’UE € 6 807 867,50

Coordonné par

TECHNISCHE UNIVERSITEIT EINDHOVEN
Netherlands

Livrables

Demonstrators, pilots, prototypes (16)

Chip compatible culture protocol for functional midbrain/hindbrain generation
Finalizing the activity and transport measurement in multiple compartment iPSC derived cultures
Demonstration of electrode integration with silicon sieve device
Electrophysiological characterization of control and patient-derived midbrain organoids.
Array device demonstration
Characterization of network activity by fluorescence-based calcium imaging.
Characterization of iPSC derived ENS networks using Ca2+ and axonal transport imaging.
Individual electrode chemical and electrochemical characterization
Floating organoids for experiments at the partners’ lab in ErasmusMC, USFD and KULeuven
Optimized protocol for the differentiation of hPSC-derived vagal NC toward enteric neurons
Neuron compatibility study of electrodes
Demonstration of integrated electrode array in a fluidic device
Specifications of preferred surface chemistry of an electrode for a chosen analyte
Microsieve MEA chip
Finalizing the explorative measurements concerning α-synuclein transport and laser-induced lesions in iPSC derived ENS
Evaluation of the utility of the intestinal organoid/ENS co-culture system and self-renewing vagal and trunk NC spheres.

Documents, reports (1)

Report on electrode scaling

Websites, patent fillings, videos etc. (1)

Website CONNECT online

Publications

Peer reviewed articles (16)

Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives

Auteurs: Matthew Wind, Antigoni Gogolou, Ichcha Manipur, Ilaria Granata, Larissa Butler, Peter W. Andrews, Ivana Barbaric, Ke Ning, Mario R. Guarracino, Marysia Placzek, Anestis Tsakiridis
Publié dans: Development, Numéro 148/6, 2021, ISSN 0950-1991
Éditeur: The Company of Biologists Ltd.
DOI: 10.1242/dev.194415

Nanogrooves for 2D and 3D Microenvironments of SH-SY5Y Cultures in Brain-on-Chip Technology

Auteurs: Alex Bastiaens, Rahman Sabahi-Kaviani, Regina Luttge
Publié dans: Frontiers in Neuroscience, Numéro 14, 2020, ISSN 1662-453X
Éditeur: Frontiers Media SA
DOI: 10.3389/fnins.2020.00666

Analyzing Developing Brain-On-Chip Cultures with the CALIMA Calcium Imaging Tool

Auteurs: Elles A. L. Raaijmakers, Nikki Wanders, Rob M. C. Mestrom, Regina Luttge
Publié dans: Micromachines, Numéro 12/4, 2021, Page(s) 412, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12040412

Gut innervation and enteric nervous system development: a spatial, temporal and molecular tour de force

Auteurs: Yi-Ning Kang, Candice Fung, Pieter Vanden Berghe
Publié dans: Development, Numéro 148/3, 2021, ISSN 0950-1991
Éditeur: The Company of Biologists Ltd.
DOI: 10.1242/dev.182543

Reproducible generation of human midbrain organoids for in vitro modeling of Parkinson’s disease

Auteurs: Sarah Louise Nickels, Jennifer Modamio, Bárbara Mendes-Pinheiro, Anna Sophia Monzel, Fay Betsou, Jens Christian Schwamborn
Publié dans: Stem Cell Research, Numéro 46, 2020, Page(s) 101870, ISSN 1873-5061
Éditeur: Elsevier
DOI: 10.1016/j.scr.2020.101870

Trends in Carbon, Oxygen, and Nitrogen Core in the X-ray Absorption Spectroscopy of Carbon Nanomaterials: A Guide for the Perplexed

Auteurs: Sami Sainio, Niklas Wester, Anja Aarva, Charles J. Titus, Dennis Nordlund, Esko I. Kauppinen, Elli Leppänen, Tommi Palomäki, Jessica E. Koehne, Olli Pitkänen, Krisztian Kordas, Maria Kim, Harri Lipsanen, Miran Mozetič, Miguel A. Caro, M. Meyyappan, Jari Koskinen, Tomi Laurila
Publié dans: The Journal of Physical Chemistry C, Numéro 125/1, 2021, Page(s) 973-988, ISSN 1932-7447
Éditeur: American Chemical Society
DOI: 10.1021/acs.jpcc.0c08597

Integrated, automated maintenance, expansion and differentiation of 2D and 3D patient-derived cellular models for high throughput drug screening

Auteurs: Ibrahim Boussaad, Gérald Cruciani, Silvia Bolognin, Paul Antony, Claire M. Dording, Yong-Jun Kwon, Peter Heutink, Eugenio Fava, Jens C. Schwamborn, Rejko Krüger
Publié dans: Scientific Reports, Numéro 11/1, 2021, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-021-81129-3

Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells

Auteurs: Antigoni Gogolou, Thomas J.R. Frith, Anestis Tsakiridis
Publié dans: Current Protocols, Numéro 1/6, 2021, ISSN 2691-1299
Éditeur: Wiley
DOI: 10.1002/cpz1.137

Three-Dimensional Fine Structure of Nanometer-Scale Nafion Thin Films

Auteurs: A. Peltonen, J. Etula, J. Seitsonen, P. Engelhardt, T. Laurila
Publié dans: ACS Applied Polymer Materials, Numéro 3/2, 2021, Page(s) 1078-1086, ISSN 2637-6105
Éditeur: American Chemical Society
DOI: 10.1021/acsapm.0c01318

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Auteurs: Rahman Sabahi-Kaviani, Regina Luttge
Publié dans: Micromachines, Numéro 12/1, 2021, Page(s) 21, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Electrical monitoring approaches in 3-dimensional cell culture systems: Toward label-free, high spatiotemporal resolution, and high-content data collection in vitro

Auteurs: Yagmur Demircan Yalcin, Regina Luttge
Publié dans: Organs-on-a-Chip, Numéro 3, 2021, Page(s) 100006, ISSN 2666-1020
Éditeur: Elsevier
DOI: 10.1016/j.ooc.2021.100006

Nanogroove-Enhanced Hydrogel Scaffolds for 3D Neuronal Cell Culture: An Easy Access Brain-on-Chip Model

Auteurs: Bastiaens, Xie, Luttge
Publié dans: Micromachines, Numéro 10/10, 2019, Page(s) 638, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi10100638

Exploiting nanogroove-induced cell culture anisotropy to advance in vitro brain models

Auteurs: Alex Bastiaens, Jean-Philippe Frimat, Teun van Nunen, Regina Luttge
Publié dans: Journal of Vacuum Science & Technology B, Numéro 37/6, 2019, Page(s) 061802, ISSN 2166-2754
Éditeur: AVS Science and Technology Society
DOI: 10.1116/1.5119687

Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture

Auteurs: Gulden Akcay, Regina Luttge
Publié dans: Micromachines, Numéro 12/2, 2021, Page(s) 165, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12020165

Understanding axial progenitor biology in vivo and in vitro

Auteurs: Filip J. Wymeersch, Valerie Wilson, Anestis Tsakiridis
Publié dans: Development, Numéro 148/4, 2021, ISSN 0950-1991
Éditeur: The Company of Biologists Ltd.
DOI: 10.1242/dev.180612

3D-electrode integrated microsieve structure as a rapid and cost-effective single neuron detector

Auteurs: Yagmur Demircan Yalcin, Regina Luttge
Publié dans: Journal of Vacuum Science & Technology B, Numéro 38/6, 2020, Page(s) 063202, ISSN 2166-2754
Éditeur: AVS Science and Technology Society
DOI: 10.1116/6.0000518

Non-peer reviewed articles (1)

Microenvironments Matter: Advances in Brain-on-Chip

Auteurs: Akcay, G.; Luttge, R.
Publié dans: Biosensors, 2023, ISSN 2079-6374
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/bios13050551