Connecting neural networks: Nervous-system-on-Chip Technology

Informations projet

CONNECT

N° de convention de subvention: 824070

DOI

10.3030/824070

Projet clôturé

Date de signature de la CE 23 Octobre 2018

Date de début 1 Janvier 2019

Date de fin 30 Juin 2024

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 6 807 867,50

Contribution de l’UE

€ 6 807 867,50

Coordonné par

TECHNISCHE UNIVERSITEIT EINDHOVEN
Netherlands

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Demonstrators, pilots, prototypes (16)

Chip compatible culture protocol for functional midbrain/hindbrain generation
Finalizing the activity and transport measurement in multiple compartment iPSC derived cultures
Demonstration of electrode integration with silicon sieve device
Electrophysiological characterization of control and patient-derived midbrain organoids.
Array device demonstration
Characterization of network activity by fluorescence-based calcium imaging.
Characterization of iPSC derived ENS networks using Ca2+ and axonal transport imaging.
Individual electrode chemical and electrochemical characterization
Floating organoids for experiments at the partners’ lab in ErasmusMC, USFD and KULeuven
Optimized protocol for the differentiation of hPSC-derived vagal NC toward enteric neurons
Neuron compatibility study of electrodes
Demonstration of integrated electrode array in a fluidic device
Specifications of preferred surface chemistry of an electrode for a chosen analyte
Microsieve MEA chip
Finalizing the explorative measurements concerning α-synuclein transport and laser-induced lesions in iPSC derived ENS
Evaluation of the utility of the intestinal organoid/ENS co-culture system and self-renewing vagal and trunk NC spheres.

Documents, reports (1)

Report on electrode scaling

Websites, patent fillings, videos etc. (2)

Website CONNECT online
CONNECT Stakeholder meeting

Open Research Data Pilot (1)

Open Research Data

Publications

Other (3)

Synaptic decline precedes dopaminergic neuronal loss in human midbrain organoids harboring a triplication of the <i>SNCA</i> gene

Auteurs: Jennifer Modamio, Claudia Saraiva, Gemma Gomez Giro, Sarah Louise Nickels, Javier Jarazo, Paul Antony, Peter Barbuti, Rashi Hadler, Christian Jäger, Rejko Krüger, Enrico Glaab, Jens Christian Schwamborn
Publié dans: 2021
Éditeur: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.07.15.452499

Retinoic acid accelerates the specification of enteric neural progenitors from<i>in vitro</i>-derived neural crest

Auteurs: Thomas J.R Frith, Antigoni Gogolou, James O.S Hackland, Ivana Barbaric, Nikhil Thapar, Alan J. Burns, Peter W Andrews, Anestis Tsakiridis, Conor J. McCann
Publié dans: 2022
Éditeur: Cold Spring Harbor Laboratory
DOI: 10.1101/819748

Human pluripotent stem cells: tools for regenerative medicine.

Auteurs: Andrews, P.W.
Publié dans: Biomaterials translational, 2021
Éditeur: Chinese Medical Multimedia Press
DOI: 10.12336/biomatertransl.2021.04.004

Peer reviewed articles (45)

Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives

Auteurs: Matthew Wind, Antigoni Gogolou, Ichcha Manipur, Ilaria Granata, Larissa Butler, Peter W. Andrews, Ivana Barbaric, Ke Ning, Mario R. Guarracino, Marysia Placzek, Anestis Tsakiridis
Publié dans: Development, Numéro 148/6, 2021, ISSN 0950-1991
Éditeur: The Company of Biologists Ltd.
DOI: 10.1242/dev.194415

Microenvironments Matter: Advances in Brain-on-Chip

Auteurs: Akcay, G.; Luttge, R.
Publié dans: Biosensors, 2023, ISSN 2079-6374
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/bios13050551

Midbrain Organoids: A New Tool to Investigate Parkinson’s Disease

Auteurs: Lisa Maria Smits, Jens Christian Schwamborn
Publié dans: Frontiers in Cell and Developmental Biology, Numéro 8, 2022, ISSN 2296-634X
Éditeur: Frontiers in Cell and Developmental Biology
DOI: 10.3389/fcell.2020.00359

Local axonal morphology guides the topography of interneuron myelination in mouse and human neocortex

Auteurs: Jeffrey Stedehouder, Demi Brizee, Johan A Slotman, Maria Pascual-Garcia, Megan L Leyrer, Bibi LJ Bouwen, Clemens MF Dirven, Zhenyu Gao, David M Berson, Adriaan B Houtsmuller, Steven A Kushner
Publié dans: eLife, Numéro 8, 2023, ISSN 2050-084X
Éditeur: eLife Sciences Publications
DOI: 10.7554/elife.48615

A robust protocol for the generation of human midbrain organoids

Auteurs: Alise Zagare, Matthieu Gobin, Anna S. Monzel, Jens C. Schwamborn
Publié dans: STAR Protocols, Numéro 2, 2022, Page(s) 100524, ISSN 2666-1667
Éditeur: Elsevier
DOI: 10.1016/j.xpro.2021.100524

Rapid industrial scale synthesis of robust carbon nanotube network electrodes for electroanalysis

Auteurs: Elli Leppänen, Jarkko Etula, Peter Engelhardt, Sami Sainio, Hua Jiang, Björn Mikladal, Antti Peltonen, Ilkka Varjos, Tomi Laurila
Publié dans: Journal of Electroanalytical Chemistry, Numéro 896, 2022, Page(s) 115255, ISSN 1572-6657
Éditeur: Elsevier BV
DOI: 10.1016/j.jelechem.2021.115255

Nanogrooves for 2D and 3D Microenvironments of SH-SY5Y Cultures in Brain-on-Chip Technology

Auteurs: Alex Bastiaens, Rahman Sabahi-Kaviani, Regina Luttge
Publié dans: Frontiers in Neuroscience, Numéro 14, 2020, ISSN 1662-453X
Éditeur: Frontiers Media SA
DOI: 10.3389/fnins.2020.00666

In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells

Auteurs: Matt Wind, Anestis Tsakiridis
Publié dans: Current Protocols, Numéro 1, 2023, ISSN 2691-1299
Éditeur: John Wiley & Sons
DOI: 10.1002/cpz1.244

Analyzing Developing Brain-On-Chip Cultures with the CALIMA Calcium Imaging Tool

Auteurs: Elles A. L. Raaijmakers, Nikki Wanders, Rob M. C. Mestrom, Regina Luttge
Publié dans: Micromachines, Numéro 12/4, 2021, Page(s) 412, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12040412

Gut innervation and enteric nervous system development: a spatial, temporal and molecular tour de force

Auteurs: Yi-Ning Kang, Candice Fung, Pieter Vanden Berghe
Publié dans: Development, Numéro 148/3, 2021, ISSN 0950-1991
Éditeur: The Company of Biologists Ltd.
DOI: 10.1242/dev.182543

Real-time selective detection of dopamine and serotonin at nanomolar concentration from complex in vitro systems

Auteurs: Samuel Rantataro, Ilmari Parkkinen, Mikko Airavaara, Tomi Laurila
Publié dans: Biosensors and Bioelectronics, Numéro 241, 2023, Page(s) 115579, ISSN 0956-5663
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2023.115579

Nanofabricating neural networks: Strategies, advances, and challenges

Auteurs: Regina Luttge
Publié dans: Journal of Vacuum Science &amp; Technology B, Numéro 40, 2024, ISSN 2166-2754
Éditeur: AVS Science and Technology Society
DOI: 10.1116/6.0001649

Reproducible generation of human midbrain organoids for in vitro modeling of Parkinson’s disease

Auteurs: Sarah Louise Nickels, Jennifer Modamio, Bárbara Mendes-Pinheiro, Anna Sophia Monzel, Fay Betsou, Jens Christian Schwamborn
Publié dans: Stem Cell Research, Numéro 46, 2020, Page(s) 101870, ISSN 1873-5061
Éditeur: Elsevier
DOI: 10.1016/j.scr.2020.101870

Shape Memory Polymer-Based Insertable Electrode Array Towards Minimally Invasive Subdural Implantation

Auteurs: Anastasiia Kravtcova, Antoniya Toncheva, Samuel Rantataro, Emilia Peltola, Jean-Marie Raquez, Pierre Lambert, Quan Zhou
Publié dans: IEEE Sensors Journal, Numéro 21, 2021, Page(s) 17282-17289, ISSN 1530-437X
Éditeur: Institute of Electrical and Electronics Engineers
DOI: 10.1109/jsen.2021.3078358

Trends in Carbon, Oxygen, and Nitrogen Core in the X-ray Absorption Spectroscopy of Carbon Nanomaterials: A Guide for the Perplexed

Auteurs: Sami Sainio, Niklas Wester, Anja Aarva, Charles J. Titus, Dennis Nordlund, Esko I. Kauppinen, Elli Leppänen, Tommi Palomäki, Jessica E. Koehne, Olli Pitkänen, Krisztian Kordas, Maria Kim, Harri Lipsanen, Miran Mozetič, Miguel A. Caro, M. Meyyappan, Jari Koskinen, Tomi Laurila
Publié dans: The Journal of Physical Chemistry C, Numéro 125/1, 2021, Page(s) 973-988, ISSN 1932-7447
Éditeur: American Chemical Society
DOI: 10.1021/acs.jpcc.0c08597

Integrated, automated maintenance, expansion and differentiation of 2D and 3D patient-derived cellular models for high throughput drug screening

Auteurs: Ibrahim Boussaad, Gérald Cruciani, Silvia Bolognin, Paul Antony, Claire M. Dording, Yong-Jun Kwon, Peter Heutink, Eugenio Fava, Jens C. Schwamborn, Rejko Krüger
Publié dans: Scientific Reports, Numéro 11/1, 2021, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-021-81129-3

Human enteric nervous system progenitor transplantation improves functional responses in Hirschsprung disease patient-derived tissue

Auteurs: Benjamin Jevans, Fay Cooper, Yuliia Fatieieva, Antigoni Gogolou, Yi-Ning Kang, Restuadi Restuadi, Dale Moulding, Pieter Vanden Berghe, Igor Adameyko, Nikhil Thapar, Peter W Andrews, Paolo De Coppi, Anestis Tsakiridis, Conor J McCann
Publié dans: Gut, Numéro 73, 2024, Page(s) 1441-1453, ISSN 0017-5749
Éditeur: BMJ Publishing Group
DOI: 10.1136/gutjnl-2023-331532

Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells

Auteurs: Antigoni Gogolou, Thomas J.R. Frith, Anestis Tsakiridis
Publié dans: Current Protocols, Numéro 1/6, 2021, ISSN 2691-1299
Éditeur: Wiley
DOI: 10.1002/cpz1.137

Nanostructured Geometries Strongly Affect Fouling of Carbon Electrodes

Auteurs: Ayesha Kousar, Emilia Peltola, Tomi Laurila
Publié dans: ACS Omega, Numéro 6, 2023, Page(s) 26391-26403, ISSN 2470-1343
Éditeur: American Chemical Society
DOI: 10.1021/acsomega.1c03666

Ascorbic acid does not necessarily interfere with the electrochemical detection of dopamine

Auteurs: Samuel Rantataro, Laura Ferrer Pascual, Tomi Laurila
Publié dans: Scientific Reports, Numéro 12, 2022, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-022-24580-0

Notch signalling influences cell fate decisions and HOX gene induction in axial progenitors

Auteurs: Fay Cooper, Celine Souilhol, Scott Haston, Shona Gray, Katy Boswell, Antigoni Gogolou, Thomas J. R. Frith, Dylan Stavish, Bethany M. James, Daniel Bose, Jacqueline Kim Dale, Anestis Tsakiridis
Publié dans: Development, Numéro 151, 2024, ISSN 0950-1991
Éditeur: The Company of Biologists Ltd.
DOI: 10.1242/dev.202098

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Auteurs: Rahman Sabahi-Kaviani, Regina Luttge
Publié dans: Micromachines, Numéro 12, 2024, Page(s) 21, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Spontaneous Epileptic Recordings from hiPSC-Derived Cortical Neurons Cultured with a Human Epileptic Brain Biopsy on a Multi Electrode Array

Auteurs: Michel H. Y. Hu, Jean-Philippe Frimat, Kim Rijkers, Olaf E. M. G. Schijns, Arn M. J. M. van den Maagdenberg, Jim T. A. Dings, Regina Luttge, Govert Hoogland
Publié dans: Applied Sciences, Numéro 13, 2025, Page(s) 1432, ISSN 2076-3417
Éditeur: MDPI Applied Science
DOI: 10.3390/app13031432

Three-Dimensional Fine Structure of Nanometer-Scale Nafion Thin Films

Auteurs: A. Peltonen, J. Etula, J. Seitsonen, P. Engelhardt, T. Laurila
Publié dans: ACS Applied Polymer Materials, Numéro 3/2, 2021, Page(s) 1078-1086, ISSN 2637-6105
Éditeur: American Chemical Society
DOI: 10.1021/acsapm.0c01318

Electrochemical detection of amine neurotransmitters is drastically different in buffer solutions, in vivo, and cell culture systems

Auteurs: Samuel Rantataro, Laura Ferrer Pascual, Tomi Laurila
Publié dans: Electrochemistry Communications, 2024, Page(s) 107732, ISSN 1388-2481
Éditeur: Elsevier BV
DOI: 10.1016/j.elecom.2024.107732

Retinoic Acid Accelerates the Specification of Enteric Neural Progenitors from In-Vitro-Derived Neural Crest

Auteurs: Thomas J.R. Frith, Antigoni Gogolou, James O.S. Hackland, Zoe A. Hewitt, Harry D. Moore, Ivana Barbaric, Nikhil Thapar, Alan J. Burns, Peter W. Andrews, Anestis Tsakiridis, Conor J. McCann
Publié dans: Stem Cell Reports, Numéro 15, 2022, Page(s) 557-565, ISSN 2213-6711
Éditeur: Cell Press
DOI: 10.1016/j.stemcr.2020.07.024

A short history of pluripotent stem cells markers

Auteurs: Peter W. Andrews, Paul J. Gokhale
Publié dans: Stem Cell Reports, Numéro 19, 2024, Page(s) 1-10, ISSN 2213-6711
Éditeur: Cell Press
DOI: 10.1016/j.stemcr.2023.11.012

Stacked hydrogel-based brain-on-chips utilizing capillary force flow pinning

Auteurs: Gulden Akcay, Jeroen van Venrooij, Regina Luttge
Publié dans: Journal of Vacuum Science &amp; Technology B, Numéro 42, 2024, ISSN 2166-2754
Éditeur: AVS Science and Technology Society
DOI: 10.1116/6.0003967

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Auteurs: Rahman Sabahi-Kaviani, Regina Luttge
Publié dans: Micromachines, Numéro 12/1, 2021, Page(s) 21, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Electrical monitoring approaches in 3-dimensional cell culture systems: Toward label-free, high spatiotemporal resolution, and high-content data collection in vitro

Auteurs: Yagmur Demircan Yalcin, Regina Luttge
Publié dans: Organs-on-a-Chip, Numéro 3, 2021, Page(s) 100006, ISSN 2666-1020
Éditeur: Elsevier
DOI: 10.1016/j.ooc.2021.100006

Nanogroove-Enhanced Hydrogel Scaffolds for 3D Neuronal Cell Culture: An Easy Access Brain-on-Chip Model

Auteurs: Bastiaens, Xie, Luttge
Publié dans: Micromachines, Numéro 10/10, 2019, Page(s) 638, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi10100638

Exploiting nanogroove-induced cell culture anisotropy to advance in vitro brain models

Auteurs: Alex Bastiaens, Jean-Philippe Frimat, Teun van Nunen, Regina Luttge
Publié dans: Journal of Vacuum Science & Technology B, Numéro 37/6, 2019, Page(s) 061802, ISSN 2166-2754
Éditeur: AVS Science and Technology Society
DOI: 10.1116/1.5119687

Modeling early phenotypes of Parkinson’s disease by age-induced midbrain-striatum assembloids

Auteurs: Kyriaki Barmpa, Claudia Saraiva, Diego Lopez-Pigozzi, Gemma Gomez-Giro, Elisa Gabassi, Sarah Spitz, Konstanze Brandauer, Juan E. Rodriguez Gatica, Paul Antony, Graham Robertson, Rahman Sabahi-Kaviani, Alessandro Bellapianta, Florentia Papastefanaki, Regina Luttge, Ulrich Kubitscheck, Ahmad Salti, Peter Ertl, Mario Bortolozzi, Rebecca Matsas, Frank Edenhofer, Jens C. Schwamborn
Publié dans: Communications Biology, Numéro 7, 2024, ISSN 2399-3642
Éditeur: Communications Biology
DOI: 10.1038/s42003-024-07273-4

Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells

Auteurs: Fay Cooper, Anestis Tsakiridis
Publié dans: Biochemical Society Transactions, Numéro 50, 2022, Page(s) 499-511, ISSN 0300-5127
Éditeur: Portland Press, Ltd.
DOI: 10.1042/bst20211152

Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture

Auteurs: Gulden Akcay, Regina Luttge
Publié dans: Micromachines, Numéro 12/2, 2021, Page(s) 165, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12020165

X-ray Spectroscopy Fingerprints of Pristine and Functionalized Graphene

Auteurs: Anja Aarva, Sami Sainio, Volker L. Deringer, Miguel A. Caro, Tomi Laurila
Publié dans: The Journal of Physical Chemistry C, Numéro 125, 2023, Page(s) 18234-18246, ISSN 1932-7447
Éditeur: American Chemical Society
DOI: 10.1021/acs.jpcc.1c03238

A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations

Auteurs: Ingrid M. Saldana-Guerrero, Luis F. Montano-Gutierrez, Katy Boswell, Christoph Hafemeister, Evon Poon, Lisa E. Shaw, Dylan Stavish, Rebecca A. Lea, Sara Wernig-Zorc, Eva Bozsaky, Irfete S. Fetahu, Peter Zoescher, Ulrike Pötschger, Marie Bernkopf, Andrea Wenninger-Weinzierl, Caterina Sturtzel, Celine Souilhol, Sophia Tarelli, Mohamed R. Shoeb, Polyxeni Bozatzi, Magdalena Rados, Maria Guarini, Mich
Publié dans: Nature Communications, Numéro 15, 2024, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-024-47945-7

Microfabrication atomic layer deposited Pt NPs/TiN thin film on silicon as a nanostructure signal Transducer: Electrochemical characterization toward neurotransmitter sensing

Auteurs: Yalda Shoja, Noora Isoaho, Ville Jokinen, Sami Franssila
Publié dans: Applied Surface Science, Numéro 573, 2024, Page(s) 151444, ISSN 0169-4332
Éditeur: Elsevier BV
DOI: 10.1016/j.apsusc.2021.151444

Understanding axial progenitor biology in vivo and in vitro

Auteurs: Filip J. Wymeersch, Valerie Wilson, Anestis Tsakiridis
Publié dans: Development, Numéro 148/4, 2021, ISSN 0950-1991
Éditeur: The Company of Biologists Ltd.
DOI: 10.1242/dev.180612

The origins of human pluripotent stem cells: the road from a cancer to regenerative medicine

Auteurs: Peter W. Andrews
Publié dans: In Vitro Cellular &amp; Developmental Biology - Animal, Numéro 60, 2024, Page(s) 514-520, ISSN 1071-2690
Éditeur: Tissue Culture Association
DOI: 10.1007/s11626-024-00865-8

Computational study of mechanical stresses in a cell interacting with micromechanical cues and microfabrication of such cues in Nervous system-on-Chips

Auteurs: Rahman Sabahi-Kaviani, Daan van Boekel, Regina Luttge
Publié dans: Journal of Vacuum Science &amp; Technology B, Numéro 41, 2023, ISSN 2166-2754
Éditeur: AVS Science and Technology Society
DOI: 10.1116/6.0002291

Effect of etchant gases on the structure and properties of carbon nanofibers

Auteurs: Ishan Pande, Laura Ferrer Pascual, Ayesha Kousar, Jani Sainio, Hua Jiang, Tomi Laurila
Publié dans: Diamond and Related Materials, Numéro 144, 2024, Page(s) 111004, ISSN 0925-9635
Éditeur: Elsevier BV
DOI: 10.1016/j.diamond.2024.111004

3D-electrode integrated microsieve structure as a rapid and cost-effective single neuron detector

Auteurs: Yagmur Demircan Yalcin, Regina Luttge
Publié dans: Journal of Vacuum Science & Technology B, Numéro 38/6, 2020, Page(s) 063202, ISSN 2166-2754
Éditeur: AVS Science and Technology Society
DOI: 10.1116/6.0000518

Modulating the Geometry of the Carbon Nanofiber Electrodes Provides Control over Dopamine Sensor Performance

Auteurs: Ayesha Kousar, Ishan Pande, Laura F. Pascual, Emilia Peltola, Jani Sainio, Tomi Laurila
Publié dans: Analytical Chemistry, Numéro 95, 2023, Page(s) 2983-2991, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.2c04843

Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor

Auteurs: Antigoni Gogolou, Celine Souilhol, Ilaria Granata, Filip J Wymeersch, Ichcha Manipur, Matthew Wind, Thomas JR Frith, Maria Guarini, Alessandro Bertero, Christoph Bock, Florian Halbritter, Minoru Takasato, Mario R Guarracino, Anestis Tsakiridis
Publié dans: eLife, Numéro 11, 2023, ISSN 2050-084X
Éditeur: eLife Sciences Publications
DOI: 10.7554/elife.74263

Non-peer reviewed articles (1)

Editorial for the Special Issue on Microfluidic Brain-on-a-Chip

Auteurs: Regina Luttge
Publié dans: Micromachines, Numéro 12, 2025, Page(s) 1100, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12091100