Connecting neural networks: Nervous-system-on-Chip Technology

Informazioni relative al progetto

CONNECT

ID dell’accordo di sovvenzione: 824070

DOI

10.3030/824070

Progetto chiuso

Data della firma CE 23 Ottobre 2018

Data di avvio 1 Gennaio 2019

Data di completamento 30 Giugno 2024

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 6 807 867,50

Contributo UE

€ 6 807 867,50

Coordinato da

TECHNISCHE UNIVERSITEIT EINDHOVEN
Netherlands

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Demonstrators, pilots, prototypes (16)

Chip compatible culture protocol for functional midbrain/hindbrain generation
Finalizing the activity and transport measurement in multiple compartment iPSC derived cultures
Demonstration of electrode integration with silicon sieve device
Electrophysiological characterization of control and patient-derived midbrain organoids.
Array device demonstration
Characterization of network activity by fluorescence-based calcium imaging.
Characterization of iPSC derived ENS networks using Ca2+ and axonal transport imaging.
Individual electrode chemical and electrochemical characterization
Floating organoids for experiments at the partners’ lab in ErasmusMC, USFD and KULeuven
Optimized protocol for the differentiation of hPSC-derived vagal NC toward enteric neurons
Neuron compatibility study of electrodes
Demonstration of integrated electrode array in a fluidic device
Specifications of preferred surface chemistry of an electrode for a chosen analyte
Microsieve MEA chip
Finalizing the explorative measurements concerning α-synuclein transport and laser-induced lesions in iPSC derived ENS
Evaluation of the utility of the intestinal organoid/ENS co-culture system and self-renewing vagal and trunk NC spheres.

Documents, reports (1)

Report on electrode scaling

Websites, patent fillings, videos etc. (2)

Website CONNECT online
CONNECT Stakeholder meeting

Open Research Data Pilot (1)

Open Research Data

Pubblicazioni

Other (3)

Synaptic decline precedes dopaminergic neuronal loss in human midbrain organoids harboring a triplication of the <i>SNCA</i> gene

Autori: Jennifer Modamio, Claudia Saraiva, Gemma Gomez Giro, Sarah Louise Nickels, Javier Jarazo, Paul Antony, Peter Barbuti, Rashi Hadler, Christian Jäger, Rejko Krüger, Enrico Glaab, Jens Christian Schwamborn
Pubblicato in: 2021
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.07.15.452499

Retinoic acid accelerates the specification of enteric neural progenitors from<i>in vitro</i>-derived neural crest

Autori: Thomas J.R Frith, Antigoni Gogolou, James O.S Hackland, Ivana Barbaric, Nikhil Thapar, Alan J. Burns, Peter W Andrews, Anestis Tsakiridis, Conor J. McCann
Pubblicato in: 2022
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/819748

Human pluripotent stem cells: tools for regenerative medicine.

Autori: Andrews, P.W.
Pubblicato in: Biomaterials translational, 2021
Editore: Chinese Medical Multimedia Press
DOI: 10.12336/biomatertransl.2021.04.004

Peer reviewed articles (45)

Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives

Autori: Matthew Wind, Antigoni Gogolou, Ichcha Manipur, Ilaria Granata, Larissa Butler, Peter W. Andrews, Ivana Barbaric, Ke Ning, Mario R. Guarracino, Marysia Placzek, Anestis Tsakiridis
Pubblicato in: Development, Numero 148/6, 2021, ISSN 0950-1991
Editore: The Company of Biologists Ltd.
DOI: 10.1242/dev.194415

Microenvironments Matter: Advances in Brain-on-Chip

Autori: Akcay, G.; Luttge, R.
Pubblicato in: Biosensors, 2023, ISSN 2079-6374
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/bios13050551

Midbrain Organoids: A New Tool to Investigate Parkinson’s Disease

Autori: Lisa Maria Smits, Jens Christian Schwamborn
Pubblicato in: Frontiers in Cell and Developmental Biology, Numero 8, 2022, ISSN 2296-634X
Editore: Frontiers in Cell and Developmental Biology
DOI: 10.3389/fcell.2020.00359

Local axonal morphology guides the topography of interneuron myelination in mouse and human neocortex

Autori: Jeffrey Stedehouder, Demi Brizee, Johan A Slotman, Maria Pascual-Garcia, Megan L Leyrer, Bibi LJ Bouwen, Clemens MF Dirven, Zhenyu Gao, David M Berson, Adriaan B Houtsmuller, Steven A Kushner
Pubblicato in: eLife, Numero 8, 2023, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.48615

A robust protocol for the generation of human midbrain organoids

Autori: Alise Zagare, Matthieu Gobin, Anna S. Monzel, Jens C. Schwamborn
Pubblicato in: STAR Protocols, Numero 2, 2022, Pagina/e 100524, ISSN 2666-1667
Editore: Elsevier
DOI: 10.1016/j.xpro.2021.100524

Rapid industrial scale synthesis of robust carbon nanotube network electrodes for electroanalysis

Autori: Elli Leppänen, Jarkko Etula, Peter Engelhardt, Sami Sainio, Hua Jiang, Björn Mikladal, Antti Peltonen, Ilkka Varjos, Tomi Laurila
Pubblicato in: Journal of Electroanalytical Chemistry, Numero 896, 2022, Pagina/e 115255, ISSN 1572-6657
Editore: Elsevier BV
DOI: 10.1016/j.jelechem.2021.115255

Nanogrooves for 2D and 3D Microenvironments of SH-SY5Y Cultures in Brain-on-Chip Technology

Autori: Alex Bastiaens, Rahman Sabahi-Kaviani, Regina Luttge
Pubblicato in: Frontiers in Neuroscience, Numero 14, 2020, ISSN 1662-453X
Editore: Frontiers Media SA
DOI: 10.3389/fnins.2020.00666

In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells

Autori: Matt Wind, Anestis Tsakiridis
Pubblicato in: Current Protocols, Numero 1, 2023, ISSN 2691-1299
Editore: John Wiley & Sons
DOI: 10.1002/cpz1.244

Analyzing Developing Brain-On-Chip Cultures with the CALIMA Calcium Imaging Tool

Autori: Elles A. L. Raaijmakers, Nikki Wanders, Rob M. C. Mestrom, Regina Luttge
Pubblicato in: Micromachines, Numero 12/4, 2021, Pagina/e 412, ISSN 2072-666X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12040412

Gut innervation and enteric nervous system development: a spatial, temporal and molecular tour de force

Autori: Yi-Ning Kang, Candice Fung, Pieter Vanden Berghe
Pubblicato in: Development, Numero 148/3, 2021, ISSN 0950-1991
Editore: The Company of Biologists Ltd.
DOI: 10.1242/dev.182543

Real-time selective detection of dopamine and serotonin at nanomolar concentration from complex in vitro systems

Autori: Samuel Rantataro, Ilmari Parkkinen, Mikko Airavaara, Tomi Laurila
Pubblicato in: Biosensors and Bioelectronics, Numero 241, 2023, Pagina/e 115579, ISSN 0956-5663
Editore: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2023.115579

Nanofabricating neural networks: Strategies, advances, and challenges

Autori: Regina Luttge
Pubblicato in: Journal of Vacuum Science &amp; Technology B, Numero 40, 2024, ISSN 2166-2754
Editore: AVS Science and Technology Society
DOI: 10.1116/6.0001649

Reproducible generation of human midbrain organoids for in vitro modeling of Parkinson’s disease

Autori: Sarah Louise Nickels, Jennifer Modamio, Bárbara Mendes-Pinheiro, Anna Sophia Monzel, Fay Betsou, Jens Christian Schwamborn
Pubblicato in: Stem Cell Research, Numero 46, 2020, Pagina/e 101870, ISSN 1873-5061
Editore: Elsevier
DOI: 10.1016/j.scr.2020.101870

Shape Memory Polymer-Based Insertable Electrode Array Towards Minimally Invasive Subdural Implantation

Autori: Anastasiia Kravtcova, Antoniya Toncheva, Samuel Rantataro, Emilia Peltola, Jean-Marie Raquez, Pierre Lambert, Quan Zhou
Pubblicato in: IEEE Sensors Journal, Numero 21, 2021, Pagina/e 17282-17289, ISSN 1530-437X
Editore: Institute of Electrical and Electronics Engineers
DOI: 10.1109/jsen.2021.3078358

Trends in Carbon, Oxygen, and Nitrogen Core in the X-ray Absorption Spectroscopy of Carbon Nanomaterials: A Guide for the Perplexed

Autori: Sami Sainio, Niklas Wester, Anja Aarva, Charles J. Titus, Dennis Nordlund, Esko I. Kauppinen, Elli Leppänen, Tommi Palomäki, Jessica E. Koehne, Olli Pitkänen, Krisztian Kordas, Maria Kim, Harri Lipsanen, Miran Mozetič, Miguel A. Caro, M. Meyyappan, Jari Koskinen, Tomi Laurila
Pubblicato in: The Journal of Physical Chemistry C, Numero 125/1, 2021, Pagina/e 973-988, ISSN 1932-7447
Editore: American Chemical Society
DOI: 10.1021/acs.jpcc.0c08597

Integrated, automated maintenance, expansion and differentiation of 2D and 3D patient-derived cellular models for high throughput drug screening

Autori: Ibrahim Boussaad, Gérald Cruciani, Silvia Bolognin, Paul Antony, Claire M. Dording, Yong-Jun Kwon, Peter Heutink, Eugenio Fava, Jens C. Schwamborn, Rejko Krüger
Pubblicato in: Scientific Reports, Numero 11/1, 2021, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-021-81129-3

Human enteric nervous system progenitor transplantation improves functional responses in Hirschsprung disease patient-derived tissue

Autori: Benjamin Jevans, Fay Cooper, Yuliia Fatieieva, Antigoni Gogolou, Yi-Ning Kang, Restuadi Restuadi, Dale Moulding, Pieter Vanden Berghe, Igor Adameyko, Nikhil Thapar, Peter W Andrews, Paolo De Coppi, Anestis Tsakiridis, Conor J McCann
Pubblicato in: Gut, Numero 73, 2024, Pagina/e 1441-1453, ISSN 0017-5749
Editore: BMJ Publishing Group
DOI: 10.1136/gutjnl-2023-331532

Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells

Autori: Antigoni Gogolou, Thomas J.R. Frith, Anestis Tsakiridis
Pubblicato in: Current Protocols, Numero 1/6, 2021, ISSN 2691-1299
Editore: Wiley
DOI: 10.1002/cpz1.137

Nanostructured Geometries Strongly Affect Fouling of Carbon Electrodes

Autori: Ayesha Kousar, Emilia Peltola, Tomi Laurila
Pubblicato in: ACS Omega, Numero 6, 2023, Pagina/e 26391-26403, ISSN 2470-1343
Editore: American Chemical Society
DOI: 10.1021/acsomega.1c03666

Ascorbic acid does not necessarily interfere with the electrochemical detection of dopamine

Autori: Samuel Rantataro, Laura Ferrer Pascual, Tomi Laurila
Pubblicato in: Scientific Reports, Numero 12, 2022, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-022-24580-0

Notch signalling influences cell fate decisions and HOX gene induction in axial progenitors

Autori: Fay Cooper, Celine Souilhol, Scott Haston, Shona Gray, Katy Boswell, Antigoni Gogolou, Thomas J. R. Frith, Dylan Stavish, Bethany M. James, Daniel Bose, Jacqueline Kim Dale, Anestis Tsakiridis
Pubblicato in: Development, Numero 151, 2024, ISSN 0950-1991
Editore: The Company of Biologists Ltd.
DOI: 10.1242/dev.202098

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Autori: Rahman Sabahi-Kaviani, Regina Luttge
Pubblicato in: Micromachines, Numero 12, 2024, Pagina/e 21, ISSN 2072-666X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Spontaneous Epileptic Recordings from hiPSC-Derived Cortical Neurons Cultured with a Human Epileptic Brain Biopsy on a Multi Electrode Array

Autori: Michel H. Y. Hu, Jean-Philippe Frimat, Kim Rijkers, Olaf E. M. G. Schijns, Arn M. J. M. van den Maagdenberg, Jim T. A. Dings, Regina Luttge, Govert Hoogland
Pubblicato in: Applied Sciences, Numero 13, 2025, Pagina/e 1432, ISSN 2076-3417
Editore: MDPI Applied Science
DOI: 10.3390/app13031432

Three-Dimensional Fine Structure of Nanometer-Scale Nafion Thin Films

Autori: A. Peltonen, J. Etula, J. Seitsonen, P. Engelhardt, T. Laurila
Pubblicato in: ACS Applied Polymer Materials, Numero 3/2, 2021, Pagina/e 1078-1086, ISSN 2637-6105
Editore: American Chemical Society
DOI: 10.1021/acsapm.0c01318

Electrochemical detection of amine neurotransmitters is drastically different in buffer solutions, in vivo, and cell culture systems

Autori: Samuel Rantataro, Laura Ferrer Pascual, Tomi Laurila
Pubblicato in: Electrochemistry Communications, 2024, Pagina/e 107732, ISSN 1388-2481
Editore: Elsevier BV
DOI: 10.1016/j.elecom.2024.107732

Retinoic Acid Accelerates the Specification of Enteric Neural Progenitors from In-Vitro-Derived Neural Crest

Autori: Thomas J.R. Frith, Antigoni Gogolou, James O.S. Hackland, Zoe A. Hewitt, Harry D. Moore, Ivana Barbaric, Nikhil Thapar, Alan J. Burns, Peter W. Andrews, Anestis Tsakiridis, Conor J. McCann
Pubblicato in: Stem Cell Reports, Numero 15, 2022, Pagina/e 557-565, ISSN 2213-6711
Editore: Cell Press
DOI: 10.1016/j.stemcr.2020.07.024

A short history of pluripotent stem cells markers

Autori: Peter W. Andrews, Paul J. Gokhale
Pubblicato in: Stem Cell Reports, Numero 19, 2024, Pagina/e 1-10, ISSN 2213-6711
Editore: Cell Press
DOI: 10.1016/j.stemcr.2023.11.012

Stacked hydrogel-based brain-on-chips utilizing capillary force flow pinning

Autori: Gulden Akcay, Jeroen van Venrooij, Regina Luttge
Pubblicato in: Journal of Vacuum Science &amp; Technology B, Numero 42, 2024, ISSN 2166-2754
Editore: AVS Science and Technology Society
DOI: 10.1116/6.0003967

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Autori: Rahman Sabahi-Kaviani, Regina Luttge
Pubblicato in: Micromachines, Numero 12/1, 2021, Pagina/e 21, ISSN 2072-666X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Electrical monitoring approaches in 3-dimensional cell culture systems: Toward label-free, high spatiotemporal resolution, and high-content data collection in vitro

Autori: Yagmur Demircan Yalcin, Regina Luttge
Pubblicato in: Organs-on-a-Chip, Numero 3, 2021, Pagina/e 100006, ISSN 2666-1020
Editore: Elsevier
DOI: 10.1016/j.ooc.2021.100006

Nanogroove-Enhanced Hydrogel Scaffolds for 3D Neuronal Cell Culture: An Easy Access Brain-on-Chip Model

Autori: Bastiaens, Xie, Luttge
Pubblicato in: Micromachines, Numero 10/10, 2019, Pagina/e 638, ISSN 2072-666X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi10100638

Exploiting nanogroove-induced cell culture anisotropy to advance in vitro brain models

Autori: Alex Bastiaens, Jean-Philippe Frimat, Teun van Nunen, Regina Luttge
Pubblicato in: Journal of Vacuum Science & Technology B, Numero 37/6, 2019, Pagina/e 061802, ISSN 2166-2754
Editore: AVS Science and Technology Society
DOI: 10.1116/1.5119687

Modeling early phenotypes of Parkinson’s disease by age-induced midbrain-striatum assembloids

Autori: Kyriaki Barmpa, Claudia Saraiva, Diego Lopez-Pigozzi, Gemma Gomez-Giro, Elisa Gabassi, Sarah Spitz, Konstanze Brandauer, Juan E. Rodriguez Gatica, Paul Antony, Graham Robertson, Rahman Sabahi-Kaviani, Alessandro Bellapianta, Florentia Papastefanaki, Regina Luttge, Ulrich Kubitscheck, Ahmad Salti, Peter Ertl, Mario Bortolozzi, Rebecca Matsas, Frank Edenhofer, Jens C. Schwamborn
Pubblicato in: Communications Biology, Numero 7, 2024, ISSN 2399-3642
Editore: Communications Biology
DOI: 10.1038/s42003-024-07273-4

Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells

Autori: Fay Cooper, Anestis Tsakiridis
Pubblicato in: Biochemical Society Transactions, Numero 50, 2022, Pagina/e 499-511, ISSN 0300-5127
Editore: Portland Press, Ltd.
DOI: 10.1042/bst20211152

Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture

Autori: Gulden Akcay, Regina Luttge
Pubblicato in: Micromachines, Numero 12/2, 2021, Pagina/e 165, ISSN 2072-666X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12020165

X-ray Spectroscopy Fingerprints of Pristine and Functionalized Graphene

Autori: Anja Aarva, Sami Sainio, Volker L. Deringer, Miguel A. Caro, Tomi Laurila
Pubblicato in: The Journal of Physical Chemistry C, Numero 125, 2023, Pagina/e 18234-18246, ISSN 1932-7447
Editore: American Chemical Society
DOI: 10.1021/acs.jpcc.1c03238

A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations

Autori: Ingrid M. Saldana-Guerrero, Luis F. Montano-Gutierrez, Katy Boswell, Christoph Hafemeister, Evon Poon, Lisa E. Shaw, Dylan Stavish, Rebecca A. Lea, Sara Wernig-Zorc, Eva Bozsaky, Irfete S. Fetahu, Peter Zoescher, Ulrike Pötschger, Marie Bernkopf, Andrea Wenninger-Weinzierl, Caterina Sturtzel, Celine Souilhol, Sophia Tarelli, Mohamed R. Shoeb, Polyxeni Bozatzi, Magdalena Rados, Maria Guarini, Mich
Pubblicato in: Nature Communications, Numero 15, 2024, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-024-47945-7

Microfabrication atomic layer deposited Pt NPs/TiN thin film on silicon as a nanostructure signal Transducer: Electrochemical characterization toward neurotransmitter sensing

Autori: Yalda Shoja, Noora Isoaho, Ville Jokinen, Sami Franssila
Pubblicato in: Applied Surface Science, Numero 573, 2024, Pagina/e 151444, ISSN 0169-4332
Editore: Elsevier BV
DOI: 10.1016/j.apsusc.2021.151444

Understanding axial progenitor biology in vivo and in vitro

Autori: Filip J. Wymeersch, Valerie Wilson, Anestis Tsakiridis
Pubblicato in: Development, Numero 148/4, 2021, ISSN 0950-1991
Editore: The Company of Biologists Ltd.
DOI: 10.1242/dev.180612

The origins of human pluripotent stem cells: the road from a cancer to regenerative medicine

Autori: Peter W. Andrews
Pubblicato in: In Vitro Cellular &amp; Developmental Biology - Animal, Numero 60, 2024, Pagina/e 514-520, ISSN 1071-2690
Editore: Tissue Culture Association
DOI: 10.1007/s11626-024-00865-8

Computational study of mechanical stresses in a cell interacting with micromechanical cues and microfabrication of such cues in Nervous system-on-Chips

Autori: Rahman Sabahi-Kaviani, Daan van Boekel, Regina Luttge
Pubblicato in: Journal of Vacuum Science &amp; Technology B, Numero 41, 2023, ISSN 2166-2754
Editore: AVS Science and Technology Society
DOI: 10.1116/6.0002291

Effect of etchant gases on the structure and properties of carbon nanofibers

Autori: Ishan Pande, Laura Ferrer Pascual, Ayesha Kousar, Jani Sainio, Hua Jiang, Tomi Laurila
Pubblicato in: Diamond and Related Materials, Numero 144, 2024, Pagina/e 111004, ISSN 0925-9635
Editore: Elsevier BV
DOI: 10.1016/j.diamond.2024.111004

3D-electrode integrated microsieve structure as a rapid and cost-effective single neuron detector

Autori: Yagmur Demircan Yalcin, Regina Luttge
Pubblicato in: Journal of Vacuum Science & Technology B, Numero 38/6, 2020, Pagina/e 063202, ISSN 2166-2754
Editore: AVS Science and Technology Society
DOI: 10.1116/6.0000518

Modulating the Geometry of the Carbon Nanofiber Electrodes Provides Control over Dopamine Sensor Performance

Autori: Ayesha Kousar, Ishan Pande, Laura F. Pascual, Emilia Peltola, Jani Sainio, Tomi Laurila
Pubblicato in: Analytical Chemistry, Numero 95, 2023, Pagina/e 2983-2991, ISSN 0003-2700
Editore: American Chemical Society
DOI: 10.1021/acs.analchem.2c04843

Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor

Autori: Antigoni Gogolou, Celine Souilhol, Ilaria Granata, Filip J Wymeersch, Ichcha Manipur, Matthew Wind, Thomas JR Frith, Maria Guarini, Alessandro Bertero, Christoph Bock, Florian Halbritter, Minoru Takasato, Mario R Guarracino, Anestis Tsakiridis
Pubblicato in: eLife, Numero 11, 2023, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.74263

Non-peer reviewed articles (1)

Editorial for the Special Issue on Microfluidic Brain-on-a-Chip

Autori: Regina Luttge
Pubblicato in: Micromachines, Numero 12, 2025, Pagina/e 1100, ISSN 2072-666X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12091100