Connecting neural networks: Nervous-system-on-Chip Technology

Resultado final

Chip compatible culture protocol for functional midbrain/hindbrain generation
Finalizing the activity and transport measurement in multiple compartment iPSC derived cultures
Demonstration of electrode integration with silicon sieve device
Electrophysiological characterization of control and patient-derived midbrain organoids.
Array device demonstration
Characterization of network activity by fluorescence-based calcium imaging.
Characterization of iPSC derived ENS networks using Ca2+ and axonal transport imaging.
Individual electrode chemical and electrochemical characterization
Floating organoids for experiments at the partners’ lab in ErasmusMC, USFD and KULeuven
Optimized protocol for the differentiation of hPSC-derived vagal NC toward enteric neurons
Neuron compatibility study of electrodes
Demonstration of integrated electrode array in a fluidic device
Specifications of preferred surface chemistry of an electrode for a chosen analyte
Microsieve MEA chip
Finalizing the explorative measurements concerning α-synuclein transport and laser-induced lesions in iPSC derived ENS
Evaluation of the utility of the intestinal organoid/ENS co-culture system and self-renewing vagal and trunk NC spheres.

Report on electrode scaling

Website CONNECT online

Publicaciones

Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives

Autores: Matthew Wind, Antigoni Gogolou, Ichcha Manipur, Ilaria Granata, Larissa Butler, Peter W. Andrews, Ivana Barbaric, Ke Ning, Mario R. Guarracino, Marysia Placzek, Anestis Tsakiridis
Publicado en: Development, Edición 148/6, 2021, ISSN 0950-1991
Editor: The Company of Biologists Ltd.
DOI: 10.1242/dev.194415

Nanogrooves for 2D and 3D Microenvironments of SH-SY5Y Cultures in Brain-on-Chip Technology

Autores: Alex Bastiaens, Rahman Sabahi-Kaviani, Regina Luttge
Publicado en: Frontiers in Neuroscience, Edición 14, 2020, ISSN 1662-453X
Editor: Frontiers Media SA
DOI: 10.3389/fnins.2020.00666

Analyzing Developing Brain-On-Chip Cultures with the CALIMA Calcium Imaging Tool

Autores: Elles A. L. Raaijmakers, Nikki Wanders, Rob M. C. Mestrom, Regina Luttge
Publicado en: Micromachines, Edición 12/4, 2021, Página(s) 412, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12040412

Gut innervation and enteric nervous system development: a spatial, temporal and molecular tour de force

Autores: Yi-Ning Kang, Candice Fung, Pieter Vanden Berghe
Publicado en: Development, Edición 148/3, 2021, ISSN 0950-1991
Editor: The Company of Biologists Ltd.
DOI: 10.1242/dev.182543

Reproducible generation of human midbrain organoids for in vitro modeling of Parkinson’s disease

Autores: Sarah Louise Nickels, Jennifer Modamio, Bárbara Mendes-Pinheiro, Anna Sophia Monzel, Fay Betsou, Jens Christian Schwamborn
Publicado en: Stem Cell Research, Edición 46, 2020, Página(s) 101870, ISSN 1873-5061
Editor: Elsevier
DOI: 10.1016/j.scr.2020.101870

Trends in Carbon, Oxygen, and Nitrogen Core in the X-ray Absorption Spectroscopy of Carbon Nanomaterials: A Guide for the Perplexed

Autores: Sami Sainio, Niklas Wester, Anja Aarva, Charles J. Titus, Dennis Nordlund, Esko I. Kauppinen, Elli Leppänen, Tommi Palomäki, Jessica E. Koehne, Olli Pitkänen, Krisztian Kordas, Maria Kim, Harri Lipsanen, Miran Mozetič, Miguel A. Caro, M. Meyyappan, Jari Koskinen, Tomi Laurila
Publicado en: The Journal of Physical Chemistry C, Edición 125/1, 2021, Página(s) 973-988, ISSN 1932-7447
Editor: American Chemical Society
DOI: 10.1021/acs.jpcc.0c08597

Integrated, automated maintenance, expansion and differentiation of 2D and 3D patient-derived cellular models for high throughput drug screening

Autores: Ibrahim Boussaad, Gérald Cruciani, Silvia Bolognin, Paul Antony, Claire M. Dording, Yong-Jun Kwon, Peter Heutink, Eugenio Fava, Jens C. Schwamborn, Rejko Krüger
Publicado en: Scientific Reports, Edición 11/1, 2021, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-021-81129-3

Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells

Autores: Antigoni Gogolou, Thomas J.R. Frith, Anestis Tsakiridis
Publicado en: Current Protocols, Edición 1/6, 2021, ISSN 2691-1299
Editor: Wiley
DOI: 10.1002/cpz1.137

Three-Dimensional Fine Structure of Nanometer-Scale Nafion Thin Films

Autores: A. Peltonen, J. Etula, J. Seitsonen, P. Engelhardt, T. Laurila
Publicado en: ACS Applied Polymer Materials, Edición 3/2, 2021, Página(s) 1078-1086, ISSN 2637-6105
Editor: American Chemical Society
DOI: 10.1021/acsapm.0c01318

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Autores: Rahman Sabahi-Kaviani, Regina Luttge
Publicado en: Micromachines, Edición 12/1, 2021, Página(s) 21, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Electrical monitoring approaches in 3-dimensional cell culture systems: Toward label-free, high spatiotemporal resolution, and high-content data collection in vitro

Autores: Yagmur Demircan Yalcin, Regina Luttge
Publicado en: Organs-on-a-Chip, Edición 3, 2021, Página(s) 100006, ISSN 2666-1020
Editor: Elsevier
DOI: 10.1016/j.ooc.2021.100006

Nanogroove-Enhanced Hydrogel Scaffolds for 3D Neuronal Cell Culture: An Easy Access Brain-on-Chip Model

Autores: Bastiaens, Xie, Luttge
Publicado en: Micromachines, Edición 10/10, 2019, Página(s) 638, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi10100638

Exploiting nanogroove-induced cell culture anisotropy to advance in vitro brain models

Autores: Alex Bastiaens, Jean-Philippe Frimat, Teun van Nunen, Regina Luttge
Publicado en: Journal of Vacuum Science & Technology B, Edición 37/6, 2019, Página(s) 061802, ISSN 2166-2754
Editor: AVS Science and Technology Society
DOI: 10.1116/1.5119687

Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture

Autores: Gulden Akcay, Regina Luttge
Publicado en: Micromachines, Edición 12/2, 2021, Página(s) 165, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12020165

Understanding axial progenitor biology in vivo and in vitro

Autores: Filip J. Wymeersch, Valerie Wilson, Anestis Tsakiridis
Publicado en: Development, Edición 148/4, 2021, ISSN 0950-1991
Editor: The Company of Biologists Ltd.
DOI: 10.1242/dev.180612

3D-electrode integrated microsieve structure as a rapid and cost-effective single neuron detector

Autores: Yagmur Demircan Yalcin, Regina Luttge
Publicado en: Journal of Vacuum Science & Technology B, Edición 38/6, 2020, Página(s) 063202, ISSN 2166-2754
Editor: AVS Science and Technology Society
DOI: 10.1116/6.0000518

Microenvironments Matter: Advances in Brain-on-Chip

Autores: Akcay, G.; Luttge, R.
Publicado en: Biosensors, 2023, ISSN 2079-6374
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/bios13050551

Buscando datos de OpenAIRE...

Resultado final

Publicaciones

Compartir esta página

Descargar