Connecting neural networks: Nervous-system-on-Chip Technology

Información del proyecto

CONNECT

Identificador del acuerdo de subvención: 824070

DOI

10.3030/824070

Proyecto cerrado

Fecha de la firma de la CE 23 Octubre 2018

Fecha de inicio 1 Enero 2019

Fecha de finalización 30 Junio 2024

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 6 807 867,50

Aportación de la UE

€ 6 807 867,50

Coordinado por

TECHNISCHE UNIVERSITEIT EINDHOVEN
Netherlands

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Demonstrators, pilots, prototypes (16)

Chip compatible culture protocol for functional midbrain/hindbrain generation
Finalizing the activity and transport measurement in multiple compartment iPSC derived cultures
Demonstration of electrode integration with silicon sieve device
Electrophysiological characterization of control and patient-derived midbrain organoids.
Array device demonstration
Characterization of network activity by fluorescence-based calcium imaging.
Characterization of iPSC derived ENS networks using Ca2+ and axonal transport imaging.
Individual electrode chemical and electrochemical characterization
Floating organoids for experiments at the partners’ lab in ErasmusMC, USFD and KULeuven
Optimized protocol for the differentiation of hPSC-derived vagal NC toward enteric neurons
Neuron compatibility study of electrodes
Demonstration of integrated electrode array in a fluidic device
Specifications of preferred surface chemistry of an electrode for a chosen analyte
Microsieve MEA chip
Finalizing the explorative measurements concerning α-synuclein transport and laser-induced lesions in iPSC derived ENS
Evaluation of the utility of the intestinal organoid/ENS co-culture system and self-renewing vagal and trunk NC spheres.

Documents, reports (1)

Report on electrode scaling

Websites, patent fillings, videos etc. (2)

Website CONNECT online
CONNECT Stakeholder meeting

Open Research Data Pilot (1)

Open Research Data

Publicaciones

Other (3)

Synaptic decline precedes dopaminergic neuronal loss in human midbrain organoids harboring a triplication of the <i>SNCA</i> gene

Autores: Jennifer Modamio, Claudia Saraiva, Gemma Gomez Giro, Sarah Louise Nickels, Javier Jarazo, Paul Antony, Peter Barbuti, Rashi Hadler, Christian Jäger, Rejko Krüger, Enrico Glaab, Jens Christian Schwamborn
Publicado en: 2021
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.07.15.452499

Retinoic acid accelerates the specification of enteric neural progenitors from<i>in vitro</i>-derived neural crest

Autores: Thomas J.R Frith, Antigoni Gogolou, James O.S Hackland, Ivana Barbaric, Nikhil Thapar, Alan J. Burns, Peter W Andrews, Anestis Tsakiridis, Conor J. McCann
Publicado en: 2022
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/819748

Human pluripotent stem cells: tools for regenerative medicine.

Autores: Andrews, P.W.
Publicado en: Biomaterials translational, 2021
Editor: Chinese Medical Multimedia Press
DOI: 10.12336/biomatertransl.2021.04.004

Peer reviewed articles (45)

Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives

Autores: Matthew Wind, Antigoni Gogolou, Ichcha Manipur, Ilaria Granata, Larissa Butler, Peter W. Andrews, Ivana Barbaric, Ke Ning, Mario R. Guarracino, Marysia Placzek, Anestis Tsakiridis
Publicado en: Development, Edición 148/6, 2021, ISSN 0950-1991
Editor: The Company of Biologists Ltd.
DOI: 10.1242/dev.194415

Microenvironments Matter: Advances in Brain-on-Chip

Autores: Akcay, G.; Luttge, R.
Publicado en: Biosensors, 2023, ISSN 2079-6374
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/bios13050551

Midbrain Organoids: A New Tool to Investigate Parkinson’s Disease

Autores: Lisa Maria Smits, Jens Christian Schwamborn
Publicado en: Frontiers in Cell and Developmental Biology, Edición 8, 2022, ISSN 2296-634X
Editor: Frontiers in Cell and Developmental Biology
DOI: 10.3389/fcell.2020.00359

Local axonal morphology guides the topography of interneuron myelination in mouse and human neocortex

Autores: Jeffrey Stedehouder, Demi Brizee, Johan A Slotman, Maria Pascual-Garcia, Megan L Leyrer, Bibi LJ Bouwen, Clemens MF Dirven, Zhenyu Gao, David M Berson, Adriaan B Houtsmuller, Steven A Kushner
Publicado en: eLife, Edición 8, 2023, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.48615

A robust protocol for the generation of human midbrain organoids

Autores: Alise Zagare, Matthieu Gobin, Anna S. Monzel, Jens C. Schwamborn
Publicado en: STAR Protocols, Edición 2, 2022, Página(s) 100524, ISSN 2666-1667
Editor: Elsevier
DOI: 10.1016/j.xpro.2021.100524

Rapid industrial scale synthesis of robust carbon nanotube network electrodes for electroanalysis

Autores: Elli Leppänen, Jarkko Etula, Peter Engelhardt, Sami Sainio, Hua Jiang, Björn Mikladal, Antti Peltonen, Ilkka Varjos, Tomi Laurila
Publicado en: Journal of Electroanalytical Chemistry, Edición 896, 2022, Página(s) 115255, ISSN 1572-6657
Editor: Elsevier BV
DOI: 10.1016/j.jelechem.2021.115255

Nanogrooves for 2D and 3D Microenvironments of SH-SY5Y Cultures in Brain-on-Chip Technology

Autores: Alex Bastiaens, Rahman Sabahi-Kaviani, Regina Luttge
Publicado en: Frontiers in Neuroscience, Edición 14, 2020, ISSN 1662-453X
Editor: Frontiers Media SA
DOI: 10.3389/fnins.2020.00666

In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells

Autores: Matt Wind, Anestis Tsakiridis
Publicado en: Current Protocols, Edición 1, 2023, ISSN 2691-1299
Editor: John Wiley & Sons
DOI: 10.1002/cpz1.244

Analyzing Developing Brain-On-Chip Cultures with the CALIMA Calcium Imaging Tool

Autores: Elles A. L. Raaijmakers, Nikki Wanders, Rob M. C. Mestrom, Regina Luttge
Publicado en: Micromachines, Edición 12/4, 2021, Página(s) 412, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12040412

Gut innervation and enteric nervous system development: a spatial, temporal and molecular tour de force

Autores: Yi-Ning Kang, Candice Fung, Pieter Vanden Berghe
Publicado en: Development, Edición 148/3, 2021, ISSN 0950-1991
Editor: The Company of Biologists Ltd.
DOI: 10.1242/dev.182543

Real-time selective detection of dopamine and serotonin at nanomolar concentration from complex in vitro systems

Autores: Samuel Rantataro, Ilmari Parkkinen, Mikko Airavaara, Tomi Laurila
Publicado en: Biosensors and Bioelectronics, Edición 241, 2023, Página(s) 115579, ISSN 0956-5663
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2023.115579

Nanofabricating neural networks: Strategies, advances, and challenges

Autores: Regina Luttge
Publicado en: Journal of Vacuum Science &amp; Technology B, Edición 40, 2024, ISSN 2166-2754
Editor: AVS Science and Technology Society
DOI: 10.1116/6.0001649

Reproducible generation of human midbrain organoids for in vitro modeling of Parkinson’s disease

Autores: Sarah Louise Nickels, Jennifer Modamio, Bárbara Mendes-Pinheiro, Anna Sophia Monzel, Fay Betsou, Jens Christian Schwamborn
Publicado en: Stem Cell Research, Edición 46, 2020, Página(s) 101870, ISSN 1873-5061
Editor: Elsevier
DOI: 10.1016/j.scr.2020.101870

Shape Memory Polymer-Based Insertable Electrode Array Towards Minimally Invasive Subdural Implantation

Autores: Anastasiia Kravtcova, Antoniya Toncheva, Samuel Rantataro, Emilia Peltola, Jean-Marie Raquez, Pierre Lambert, Quan Zhou
Publicado en: IEEE Sensors Journal, Edición 21, 2021, Página(s) 17282-17289, ISSN 1530-437X
Editor: Institute of Electrical and Electronics Engineers
DOI: 10.1109/jsen.2021.3078358

Trends in Carbon, Oxygen, and Nitrogen Core in the X-ray Absorption Spectroscopy of Carbon Nanomaterials: A Guide for the Perplexed

Autores: Sami Sainio, Niklas Wester, Anja Aarva, Charles J. Titus, Dennis Nordlund, Esko I. Kauppinen, Elli Leppänen, Tommi Palomäki, Jessica E. Koehne, Olli Pitkänen, Krisztian Kordas, Maria Kim, Harri Lipsanen, Miran Mozetič, Miguel A. Caro, M. Meyyappan, Jari Koskinen, Tomi Laurila
Publicado en: The Journal of Physical Chemistry C, Edición 125/1, 2021, Página(s) 973-988, ISSN 1932-7447
Editor: American Chemical Society
DOI: 10.1021/acs.jpcc.0c08597

Integrated, automated maintenance, expansion and differentiation of 2D and 3D patient-derived cellular models for high throughput drug screening

Autores: Ibrahim Boussaad, Gérald Cruciani, Silvia Bolognin, Paul Antony, Claire M. Dording, Yong-Jun Kwon, Peter Heutink, Eugenio Fava, Jens C. Schwamborn, Rejko Krüger
Publicado en: Scientific Reports, Edición 11/1, 2021, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-021-81129-3

Human enteric nervous system progenitor transplantation improves functional responses in Hirschsprung disease patient-derived tissue

Autores: Benjamin Jevans, Fay Cooper, Yuliia Fatieieva, Antigoni Gogolou, Yi-Ning Kang, Restuadi Restuadi, Dale Moulding, Pieter Vanden Berghe, Igor Adameyko, Nikhil Thapar, Peter W Andrews, Paolo De Coppi, Anestis Tsakiridis, Conor J McCann
Publicado en: Gut, Edición 73, 2024, Página(s) 1441-1453, ISSN 0017-5749
Editor: BMJ Publishing Group
DOI: 10.1136/gutjnl-2023-331532

Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells

Autores: Antigoni Gogolou, Thomas J.R. Frith, Anestis Tsakiridis
Publicado en: Current Protocols, Edición 1/6, 2021, ISSN 2691-1299
Editor: Wiley
DOI: 10.1002/cpz1.137

Nanostructured Geometries Strongly Affect Fouling of Carbon Electrodes

Autores: Ayesha Kousar, Emilia Peltola, Tomi Laurila
Publicado en: ACS Omega, Edición 6, 2023, Página(s) 26391-26403, ISSN 2470-1343
Editor: American Chemical Society
DOI: 10.1021/acsomega.1c03666

Ascorbic acid does not necessarily interfere with the electrochemical detection of dopamine

Autores: Samuel Rantataro, Laura Ferrer Pascual, Tomi Laurila
Publicado en: Scientific Reports, Edición 12, 2022, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-022-24580-0

Notch signalling influences cell fate decisions and HOX gene induction in axial progenitors

Autores: Fay Cooper, Celine Souilhol, Scott Haston, Shona Gray, Katy Boswell, Antigoni Gogolou, Thomas J. R. Frith, Dylan Stavish, Bethany M. James, Daniel Bose, Jacqueline Kim Dale, Anestis Tsakiridis
Publicado en: Development, Edición 151, 2024, ISSN 0950-1991
Editor: The Company of Biologists Ltd.
DOI: 10.1242/dev.202098

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Autores: Rahman Sabahi-Kaviani, Regina Luttge
Publicado en: Micromachines, Edición 12, 2024, Página(s) 21, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Spontaneous Epileptic Recordings from hiPSC-Derived Cortical Neurons Cultured with a Human Epileptic Brain Biopsy on a Multi Electrode Array

Autores: Michel H. Y. Hu, Jean-Philippe Frimat, Kim Rijkers, Olaf E. M. G. Schijns, Arn M. J. M. van den Maagdenberg, Jim T. A. Dings, Regina Luttge, Govert Hoogland
Publicado en: Applied Sciences, Edición 13, 2025, Página(s) 1432, ISSN 2076-3417
Editor: MDPI Applied Science
DOI: 10.3390/app13031432

Three-Dimensional Fine Structure of Nanometer-Scale Nafion Thin Films

Autores: A. Peltonen, J. Etula, J. Seitsonen, P. Engelhardt, T. Laurila
Publicado en: ACS Applied Polymer Materials, Edición 3/2, 2021, Página(s) 1078-1086, ISSN 2637-6105
Editor: American Chemical Society
DOI: 10.1021/acsapm.0c01318

Electrochemical detection of amine neurotransmitters is drastically different in buffer solutions, in vivo, and cell culture systems

Autores: Samuel Rantataro, Laura Ferrer Pascual, Tomi Laurila
Publicado en: Electrochemistry Communications, 2024, Página(s) 107732, ISSN 1388-2481
Editor: Elsevier BV
DOI: 10.1016/j.elecom.2024.107732

Retinoic Acid Accelerates the Specification of Enteric Neural Progenitors from In-Vitro-Derived Neural Crest

Autores: Thomas J.R. Frith, Antigoni Gogolou, James O.S. Hackland, Zoe A. Hewitt, Harry D. Moore, Ivana Barbaric, Nikhil Thapar, Alan J. Burns, Peter W. Andrews, Anestis Tsakiridis, Conor J. McCann
Publicado en: Stem Cell Reports, Edición 15, 2022, Página(s) 557-565, ISSN 2213-6711
Editor: Cell Press
DOI: 10.1016/j.stemcr.2020.07.024

A short history of pluripotent stem cells markers

Autores: Peter W. Andrews, Paul J. Gokhale
Publicado en: Stem Cell Reports, Edición 19, 2024, Página(s) 1-10, ISSN 2213-6711
Editor: Cell Press
DOI: 10.1016/j.stemcr.2023.11.012

Stacked hydrogel-based brain-on-chips utilizing capillary force flow pinning

Autores: Gulden Akcay, Jeroen van Venrooij, Regina Luttge
Publicado en: Journal of Vacuum Science &amp; Technology B, Edición 42, 2024, ISSN 2166-2754
Editor: AVS Science and Technology Society
DOI: 10.1116/6.0003967

Gaining Micropattern Fidelity in an NOA81 Microsieve Laser Ablation Process

Autores: Rahman Sabahi-Kaviani, Regina Luttge
Publicado en: Micromachines, Edición 12/1, 2021, Página(s) 21, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010021

Electrical monitoring approaches in 3-dimensional cell culture systems: Toward label-free, high spatiotemporal resolution, and high-content data collection in vitro

Autores: Yagmur Demircan Yalcin, Regina Luttge
Publicado en: Organs-on-a-Chip, Edición 3, 2021, Página(s) 100006, ISSN 2666-1020
Editor: Elsevier
DOI: 10.1016/j.ooc.2021.100006

Nanogroove-Enhanced Hydrogel Scaffolds for 3D Neuronal Cell Culture: An Easy Access Brain-on-Chip Model

Autores: Bastiaens, Xie, Luttge
Publicado en: Micromachines, Edición 10/10, 2019, Página(s) 638, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi10100638

Exploiting nanogroove-induced cell culture anisotropy to advance in vitro brain models

Autores: Alex Bastiaens, Jean-Philippe Frimat, Teun van Nunen, Regina Luttge
Publicado en: Journal of Vacuum Science & Technology B, Edición 37/6, 2019, Página(s) 061802, ISSN 2166-2754
Editor: AVS Science and Technology Society
DOI: 10.1116/1.5119687

Modeling early phenotypes of Parkinson’s disease by age-induced midbrain-striatum assembloids

Autores: Kyriaki Barmpa, Claudia Saraiva, Diego Lopez-Pigozzi, Gemma Gomez-Giro, Elisa Gabassi, Sarah Spitz, Konstanze Brandauer, Juan E. Rodriguez Gatica, Paul Antony, Graham Robertson, Rahman Sabahi-Kaviani, Alessandro Bellapianta, Florentia Papastefanaki, Regina Luttge, Ulrich Kubitscheck, Ahmad Salti, Peter Ertl, Mario Bortolozzi, Rebecca Matsas, Frank Edenhofer, Jens C. Schwamborn
Publicado en: Communications Biology, Edición 7, 2024, ISSN 2399-3642
Editor: Communications Biology
DOI: 10.1038/s42003-024-07273-4

Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells

Autores: Fay Cooper, Anestis Tsakiridis
Publicado en: Biochemical Society Transactions, Edición 50, 2022, Página(s) 499-511, ISSN 0300-5127
Editor: Portland Press, Ltd.
DOI: 10.1042/bst20211152

Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture

Autores: Gulden Akcay, Regina Luttge
Publicado en: Micromachines, Edición 12/2, 2021, Página(s) 165, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12020165

X-ray Spectroscopy Fingerprints of Pristine and Functionalized Graphene

Autores: Anja Aarva, Sami Sainio, Volker L. Deringer, Miguel A. Caro, Tomi Laurila
Publicado en: The Journal of Physical Chemistry C, Edición 125, 2023, Página(s) 18234-18246, ISSN 1932-7447
Editor: American Chemical Society
DOI: 10.1021/acs.jpcc.1c03238

A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations

Autores: Ingrid M. Saldana-Guerrero, Luis F. Montano-Gutierrez, Katy Boswell, Christoph Hafemeister, Evon Poon, Lisa E. Shaw, Dylan Stavish, Rebecca A. Lea, Sara Wernig-Zorc, Eva Bozsaky, Irfete S. Fetahu, Peter Zoescher, Ulrike Pötschger, Marie Bernkopf, Andrea Wenninger-Weinzierl, Caterina Sturtzel, Celine Souilhol, Sophia Tarelli, Mohamed R. Shoeb, Polyxeni Bozatzi, Magdalena Rados, Maria Guarini, Mich
Publicado en: Nature Communications, Edición 15, 2024, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-024-47945-7

Microfabrication atomic layer deposited Pt NPs/TiN thin film on silicon as a nanostructure signal Transducer: Electrochemical characterization toward neurotransmitter sensing

Autores: Yalda Shoja, Noora Isoaho, Ville Jokinen, Sami Franssila
Publicado en: Applied Surface Science, Edición 573, 2024, Página(s) 151444, ISSN 0169-4332
Editor: Elsevier BV
DOI: 10.1016/j.apsusc.2021.151444

Understanding axial progenitor biology in vivo and in vitro

Autores: Filip J. Wymeersch, Valerie Wilson, Anestis Tsakiridis
Publicado en: Development, Edición 148/4, 2021, ISSN 0950-1991
Editor: The Company of Biologists Ltd.
DOI: 10.1242/dev.180612

The origins of human pluripotent stem cells: the road from a cancer to regenerative medicine

Autores: Peter W. Andrews
Publicado en: In Vitro Cellular &amp; Developmental Biology - Animal, Edición 60, 2024, Página(s) 514-520, ISSN 1071-2690
Editor: Tissue Culture Association
DOI: 10.1007/s11626-024-00865-8

Computational study of mechanical stresses in a cell interacting with micromechanical cues and microfabrication of such cues in Nervous system-on-Chips

Autores: Rahman Sabahi-Kaviani, Daan van Boekel, Regina Luttge
Publicado en: Journal of Vacuum Science &amp; Technology B, Edición 41, 2023, ISSN 2166-2754
Editor: AVS Science and Technology Society
DOI: 10.1116/6.0002291

Effect of etchant gases on the structure and properties of carbon nanofibers

Autores: Ishan Pande, Laura Ferrer Pascual, Ayesha Kousar, Jani Sainio, Hua Jiang, Tomi Laurila
Publicado en: Diamond and Related Materials, Edición 144, 2024, Página(s) 111004, ISSN 0925-9635
Editor: Elsevier BV
DOI: 10.1016/j.diamond.2024.111004

3D-electrode integrated microsieve structure as a rapid and cost-effective single neuron detector

Autores: Yagmur Demircan Yalcin, Regina Luttge
Publicado en: Journal of Vacuum Science & Technology B, Edición 38/6, 2020, Página(s) 063202, ISSN 2166-2754
Editor: AVS Science and Technology Society
DOI: 10.1116/6.0000518

Modulating the Geometry of the Carbon Nanofiber Electrodes Provides Control over Dopamine Sensor Performance

Autores: Ayesha Kousar, Ishan Pande, Laura F. Pascual, Emilia Peltola, Jani Sainio, Tomi Laurila
Publicado en: Analytical Chemistry, Edición 95, 2023, Página(s) 2983-2991, ISSN 0003-2700
Editor: American Chemical Society
DOI: 10.1021/acs.analchem.2c04843

Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor

Autores: Antigoni Gogolou, Celine Souilhol, Ilaria Granata, Filip J Wymeersch, Ichcha Manipur, Matthew Wind, Thomas JR Frith, Maria Guarini, Alessandro Bertero, Christoph Bock, Florian Halbritter, Minoru Takasato, Mario R Guarracino, Anestis Tsakiridis
Publicado en: eLife, Edición 11, 2023, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.74263

Non-peer reviewed articles (1)

Editorial for the Special Issue on Microfluidic Brain-on-a-Chip

Autores: Regina Luttge
Publicado en: Micromachines, Edición 12, 2025, Página(s) 1100, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12091100