Descripción del proyecto
Nuevo sistema bioelectrónico unimolecular para la detección clínica de biomarcadores
Permitir la detección simultánea de varios biomarcadores mejorará la sensibilidad de las pruebas y de las conclusiones diagnósticas. Además, los biomarcadores deberían detectarse con la sensibilidad analítica más elevada posible ya que no suelen estar presentes en las poblaciones celulares, y una molécula individual puede revelar la aparición de un estado patológico. El equipo del proyecto SiMBiT, financiado con fondos europeos, aspira a desarrollar un sistema bioelectrónico que permita detectar una molécula individual de biomarcadores de proteínas y de ADN. El objetivo es el desarrollo de un prototipo de matriz de multiplexación portátil rentable y de laboratorio que produzca resultados rápidos. SiMBiT se empleará para la detección temprana de neoplasias pancreáticas humanas, la realización de análisis simultáneos de marcadores genómicos y proteicos con un volumen de muestra mínimo.
Objetivo
Digitizing biomarkers analysis by quantifying them at the single-molecule level is the new frontier for advancing the science of precision health. The SiMBiT project will develop a bio-electronic smart system leveraging on an existing lab-based proof-of-concept that can perform single-molecule detection of both proteins and DNA bio-markers. Specifically, the SiMBiT activities will develop the lab-based device into a cost-effective portable multiplexing array prototype that integrates, with a modular approach, novel materials and standard components/interfaces. The SiMBiT platform exhibits enhanced sensing capabilities: specificity towards both genomic and protein markers along with single-molecule detection limits and time-to-results within two hours. This makes the SiMBiT prototype the world best performing bio-electronic sensing system ever. SiMBiT will reach these ambitious goals with a multidisciplinary research effort involving device-physicists, analytical-chemists, bio-chemists, clinicians, electronic- and system-engineers. The platform is also single-use and cost-effective and can work in low-resource settings. The SiMBiT field-effect sensing system will be fabricated by means of future mass-manufacturable, large-area compatible, scalable techniques such as printing and other direct-writing processes. 3D printing of a module is also foreseen. The SiMBiT prototype will demonstrate, for first time, a matrix of up to 96 bio-electronic sensors and a Si IC chip for the processing of all data coming from the matrix, multiplexing single-molecule detection. As the Si IC pins are limited the chip area is reduced and its cost minimized, enabling a single-use assay plate. SiMBiT will apply the multiplexing single molecule technology to the early detection of human pancreatic neoplasms in a well-defined clinical context, performing simultaneous analysis of genomic and protein markers with a minimal sample volume, reduced costs and reduced time-to-results.
Ámbito científico
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensors
- medical and health sciencesclinical medicineoncologypancreatic cancer
Palabras clave
Programa(s)
Convocatoria de propuestas
Consulte otros proyectos de esta convocatoriaConvocatoria de subcontratación
H2020-ICT-2018-2
Régimen de financiación
RIA - Research and Innovation actionCoordinador
50019 Firenze
Italia