Projektbeschreibung
Neuartiges bioelektronisches Einzelmolekül-System für den Nachweis klinischer Biomarker
Die gleichzeitige Erkennung mehrerer Biomarker verbessert die Empfindlichkeit von Tests und diagnostischen Rückschlüssen. Darüber hinaus sollten Biomarker mit der höchstmöglichen analytischen Empfindlichkeit gemessen werden, da sie in der Regel nicht in Zellpopulationen vorhanden sind und ein einziges Molekül den Beginn eines Krankheitsbildes anzeigen kann. Das EU-finanzierte Projekt SiMBiT zielt darauf ab, ein bioelektronisches System zu entwickeln, das den Einzelmolekül-Nachweis sowohl von Proteinen als auch von DNA-Biomarkern ermöglicht. Ziel ist die Entwicklung eines laborgestützten und kostengünstigen tragbaren Prototyps von Multiplex-Arrays, der schnelle Ergebnisse liefert. SiMBiT soll bei der Früherkennung menschlicher Bauchspeicheldrüsenneoplasmen zum Einsatz kommen und die gleichzeitige Analyse von Genom- und Proteinmarkern mit einem minimalen Probenvolumen durchführen.
Ziel
Digitizing biomarkers analysis by quantifying them at the single-molecule level is the new frontier for advancing the science of precision health. The SiMBiT project will develop a bio-electronic smart system leveraging on an existing lab-based proof-of-concept that can perform single-molecule detection of both proteins and DNA bio-markers. Specifically, the SiMBiT activities will develop the lab-based device into a cost-effective portable multiplexing array prototype that integrates, with a modular approach, novel materials and standard components/interfaces. The SiMBiT platform exhibits enhanced sensing capabilities: specificity towards both genomic and protein markers along with single-molecule detection limits and time-to-results within two hours. This makes the SiMBiT prototype the world best performing bio-electronic sensing system ever. SiMBiT will reach these ambitious goals with a multidisciplinary research effort involving device-physicists, analytical-chemists, bio-chemists, clinicians, electronic- and system-engineers. The platform is also single-use and cost-effective and can work in low-resource settings. The SiMBiT field-effect sensing system will be fabricated by means of future mass-manufacturable, large-area compatible, scalable techniques such as printing and other direct-writing processes. 3D printing of a module is also foreseen. The SiMBiT prototype will demonstrate, for first time, a matrix of up to 96 bio-electronic sensors and a Si IC chip for the processing of all data coming from the matrix, multiplexing single-molecule detection. As the Si IC pins are limited the chip area is reduced and its cost minimized, enabling a single-use assay plate. SiMBiT will apply the multiplexing single molecule technology to the early detection of human pancreatic neoplasms in a well-defined clinical context, performing simultaneous analysis of genomic and protein markers with a minimal sample volume, reduced costs and reduced time-to-results.
Wissenschaftliches Gebiet
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensors
- medical and health sciencesclinical medicineoncologypancreatic cancer
Schlüsselbegriffe
Programm/Programme
Thema/Themen
Aufforderung zur Vorschlagseinreichung
Andere Projekte für diesen Aufruf anzeigenUnterauftrag
H2020-ICT-2018-2
Finanzierungsplan
RIA - Research and Innovation actionKoordinator
50019 Firenze
Italien