Descrizione del progetto
Un nuovo sistema bioelettronico a singola molecola per il rilevamento di biomarcatori ad uso clinico
Il rilevamento di vari biomarcatori in modo simultaneo permetterà di migliorare la sensibilità dei test e le conclusioni diagnostiche. Inoltre, tale rilevamento dovrebbe avvenire con la maggiore sensibilità analitica possibile, in quanto in genere essi non sono presenti nelle popolazioni di cellule e una sola molecola può rivelare l’insorgenza di una condizione patologica. Il progetto SiMBiT, finanziato dall’UE, intende sviluppare un sistema bioelettronico per consentire il rilevamento a singola molecola dei biomarcatori di proteine e DNA. L’obiettivo è quello di sviluppare un prototipo di sistema portatile di multiplazione a base laboratoriale ed economico, in grado di produrre risultati rapidi. SiMBiT sarà utilizzato per il rilevamento precoce delle neoplasie pancreatiche umane, grazie alla conduzione simultanea di analisi dei marcatori genomici e proteici su un volume minimo di campione.
Obiettivo
Digitizing biomarkers analysis by quantifying them at the single-molecule level is the new frontier for advancing the science of precision health. The SiMBiT project will develop a bio-electronic smart system leveraging on an existing lab-based proof-of-concept that can perform single-molecule detection of both proteins and DNA bio-markers. Specifically, the SiMBiT activities will develop the lab-based device into a cost-effective portable multiplexing array prototype that integrates, with a modular approach, novel materials and standard components/interfaces. The SiMBiT platform exhibits enhanced sensing capabilities: specificity towards both genomic and protein markers along with single-molecule detection limits and time-to-results within two hours. This makes the SiMBiT prototype the world best performing bio-electronic sensing system ever. SiMBiT will reach these ambitious goals with a multidisciplinary research effort involving device-physicists, analytical-chemists, bio-chemists, clinicians, electronic- and system-engineers. The platform is also single-use and cost-effective and can work in low-resource settings. The SiMBiT field-effect sensing system will be fabricated by means of future mass-manufacturable, large-area compatible, scalable techniques such as printing and other direct-writing processes. 3D printing of a module is also foreseen. The SiMBiT prototype will demonstrate, for first time, a matrix of up to 96 bio-electronic sensors and a Si IC chip for the processing of all data coming from the matrix, multiplexing single-molecule detection. As the Si IC pins are limited the chip area is reduced and its cost minimized, enabling a single-use assay plate. SiMBiT will apply the multiplexing single molecule technology to the early detection of human pancreatic neoplasms in a well-defined clinical context, performing simultaneous analysis of genomic and protein markers with a minimal sample volume, reduced costs and reduced time-to-results.
Campo scientifico
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensors
- medical and health sciencesclinical medicineoncologypancreatic cancer
Parole chiave
Programma(i)
Argomento(i)
Meccanismo di finanziamento
RIA - Research and Innovation actionCoordinatore
50019 Firenze
Italia