Reshaping undesired Inflammation in challenged Tissue Homeostasis by Next-Generation regulatory T cell (Treg) Approaches – from Advanced Technology Developments to First-in-Human Trials

Información del proyecto

RESHAPE

Identificador del acuerdo de subvención: 825392

DOI

10.3030/825392

Proyecto cerrado

Fecha de la firma de la CE 21 Noviembre 2018

Fecha de inicio 1 Enero 2019

Fecha de finalización 31 Diciembre 2024

Financiado con arreglo a

• SOCIETAL CHALLENGES - Health, demographic change and well-being

Coste total

€ 13 137 396,25

Aportación de la UE

€ 13 137 396,25

Coordinado por

CHARITE - UNIVERSITAETSMEDIZIN BERLIN
Germany

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (16)

Humanised vascularized skin Tx

Evaluation of 2 best-in-class Treg in the new humanised vascularised skin Tx model

Position paper CAR-Treg

Position /Viewpoint paper on CAR-Treg generation

RNAseq, TCRseq, Methylation report

All preseleted Treg products analysed

Position paper next generation Treg

Position paper on challenges and opportunities of nextgeneration Treg approaches

Final statement on IL-tgTreg

Final statement to the utility of IL-2tg Treg (WP1-3)

Final statement on off-the-shelf Treg

Final statement to the utility of off-the-shelf allogeneic Treg

Final statement on non-viral gene del.

Final statement to the utility of virusfree gene transfer methods for Treg

Cytometry report

All preseleted Treg products analysed

Final statement on epigenetic Treg

Final statement to the utility of epigenetically stabilised Treg

PoC rejection model on-chip

Evaluation of kidney rejection on-chip and the effects of Treg treatmentPoC

DigiWest/Luminex report

All preseleted Treg products analysed

Position paper Crispr/Cas in Tcells

Positon paper on challenges for using CRISPRCas9 modified T cells

Final statement on SPR Treg

Final statement to the utility of SPR Treg

GMP protocol for at least one tissue-specific Treg
Final statement on metabolic Treg

Final statement to the utility of mitochondrial reprogramming of Treg

Final statement on Cas9 TCR-Treg

Final statement to the utility of Cas9-specific TCR-Treg

Websites, patent fillings, videos etc. (1)

Launching website

Publicaciones

Peer reviewed articles (32)

Effector memory–type regulatory T cells display phenotypic and functional instability

Autores: Désirée Jacqueline Wendering, Leila Amini, Stephan Schlickeiser, Martí Farrera-Sal, Sarah Schulenberg, Lena Peter, Marco Mai, Tino Vollmer, Weijie Du, Maik Stein, Frederik Hamm, Alisier Malard, Carla Castro, Mingxing Yang, Ramon Ranka, Timo Rückert, Pawel Durek, Frederik Heinrich, Gilles Gasparoni, Abdulrahman Salhab, Jörn Walter, Dimitrios Laurin Wagner, Mir-Farzin Mashreghi, Sybille Landweh
Publicado en: Science Advances, Edición 10, 2024, ISSN 2375-2548
Editor: American Association for the Advancement of Science
DOI: 10.1126/sciadv.adn3470

Strong Expansion of Human Regulatory T Cells for Adoptive Cell Therapy Results in Epigenetic Changes Which May Impact Their Survival and Function

Autores: Kristy Ou, Dania Hamo, Anne Schulze, Andy Roemhild, Daniel Kaiser, Gilles Gasparoni, Abdulrahman Salhab, Ghazaleh Zarrinrad, Leila Amini, Stephan Schlickeiser, Mathias Streitz, Jörn Walter, Hans-Dieter Volk, Michael Schmueck-Henneresse, Petra Reinke, Julia K Polansky
Publicado en: Frontiers in Cell and Developmental Biology, Edición 751590, 2021, ISSN 2296-634X
Editor: Frontiers
DOI: 10.3389/fcell.2021.751590

Regulatory T cells for minimising immune suppression in kidney transplantation: phase I/IIa clinical trial

Autores: Andy Roemhild, Natalie Maureen Otto, Guido Moll, Mohamed Abou-El-Enein, Daniel Kaiser, Gantuja Bold, Thomas Schachtner, Mira Choi, Robert Oellinger, Sybille Landwehr-Kenzel, Karsten Juerchott, Birgit Sawitzki, Cordula Giesler, Anett Sefrin, Carola Beier, Dimitrios Laurin Wagner, Stephan Schlickeiser, Mathias Streitz, Michael Schmueck-Henneresse, Leila Amini, Ulrik Stervbo, Nina Babel, Hans-Dieter
Publicado en: British Medical Journal, Edición 371, 2020, ISSN 2057-0066
Editor: BMJ
DOI: 10.1136/bmj.m3734

Development of Beta-Amyloid-Specific CAR-Tregs for the Treatment of Alzheimer’s Disease

Autores: Valerie Saetzler, Tobias Riet, Andrea Schienke, Pierre Henschel, Kiara Freitag, Alexander Haake, Frank L. Heppner, Laura Elisa Buitrago-Molina, Fatih Noyan, Elmar Jaeckel, Matthias Hardtke-Wolenski
Publicado en: Cells, Edición 12, 2025, Página(s) 2115, ISSN 2073-4409
Editor: https://pubmed.ncbi.nlm.nih.gov/37626926/
DOI: 10.3390/cells12162115

Advanced Therapy Medicinal Products' Translation in Europe: A Developers' Perspective

Autores: Maja Pizevska, Jaspal Kaeda, Enrico Fritsche, Hisham Elazaly, Petra Reinke, Leila Amini
Publicado en: Frontiers in Medicine, Edición 757647, 2022, ISSN 2296-858X
Editor: Frontiers
DOI: 10.3389/fmed.2022.757647

Matching or genetic engineering of HLA Class I and II facilitates successful allogeneic ‘off-the-shelf’ regulatory T cell therapy

Autores: Oliver McCallion, Weijie Du, Viktor Glaser, Kate Milward, Clemens Franke, Jonas Kath, Mikhail Valkov, Mingxing Yang, Annette Künkele, Julia K. Polansky, Michael Schmueck-Henneresse, Hans-Dieter Volk, Petra Reinke, Dimitrios L. Wagner, Joanna Hester, Fadi Issa
Publicado en: -, 2023, ISSN 2692-8205
Editor: cold spring harbor Laboratory
DOI: 10.1101/2023.08.06.551956

Tipping the balance in autoimmunity: are regulatory t cells the cause, the cure, or both?

Autores: Matthias Hardtke-Wolenski, Sybille Landwehr-Kenzel
Publicado en: Molecular and Cellular Pediatrics, Edición 11, 2024, ISSN 2194-7791
Editor: springer
DOI: 10.1186/s40348-024-00176-8

Freezing Medium Containing 5% DMSO Enhances the Cell Viability and Recovery Rate After Cryopreservation of Regulatory T Cell Products ex vivo and in vivo

Autores: Daniel Kaiser, Natalie Maureen Otto, Oliver McCallion, Henrike Hoffmann, Ghazaleh Zarrinrad, Maik Stein, Carola Beier, Isabell Matz, Marleen Herschel, Joanna Hester, Guido Moll, Fadi Issa, Petra Reinke, Andy Roemhild
Publicado en: Frontiers in Cell and Developmental Biology, Edición 750286, 2021, ISSN 2296-634X
Editor: Frontiers
DOI: 10.3389/fcell.2021.750286

Immune Monitoring for Advanced Cell Therapy Trials in Transplantation: Which Assays and When?

Autores: Helen L. Stark, Hayson C. Wang, Jasmina Kuburic, Alaa Alzhrani, Joanna Hester and Fadi Issa
Publicado en: Frontiers in Immunology, Edición 664244, 2021, ISSN 1664-3224
Editor: Frontiers
DOI: 10.3389/fimmu.2021.664244

Long-term in vitro maintenance of plasma cells in a hydrogel-enclosed human bone marrow microphysiological 3D model system

Autores: Stefania Martini, Norman Michael Drzeniek, Regina Stark, Matthias Reiner Kollert, Weijie Du, Simon Reinke, Melanie Ort, Sebastian Hardt, Iuliia Kotko, Jonas Kath, Stephan Schlickeiser, Sven Geißler, Dimitrios Laurin Wagner, Anna-Catharina Krebs, Hans-Dieter Volk
Publicado en: Biofabrication, Edición 16, 2024, Página(s) 045005, ISSN 1758-5082
Editor: IOS Press
DOI: 10.1088/1758-5090/ad5dfe

Cyclosporine A but Not Corticosteroids Support Efficacy of Ex Vivo Expanded, Adoptively Transferred Human Tregs in GvHD

Autores: Sybille Landwehr-Kenzel, Anne Zobel, Isabela Schmitt-Knosalla, Anne Forke, Henrike Hoffmann, Michael Schmueck-Henneresse, Robert Klopfleisch, Hans-Dieter Volk, Petra Reinke
Publicado en: Frontiers in Immunology, Edición 716629, 2021, ISSN 1664-3224
Editor: Frontiers
DOI: 10.3389/fimmu.2021.716629

Gene editing of CD3 epsilon to redirect regulatory T cells for adoptive T cell transfer

Autores: Weijie Du, Fatih Noyan, Oliver McCallion, Vanessa Drosdek, Jonas Kath, Viktor Glaser, Carla Fuster-Garcia, Mingxing Yang, Maik Stein, Clemens Franke, Yaolin Pu, Olaf Weber, Julia K. Polansky, Toni Cathomen, Elmar Jaeckel, Joanna Hester, Fadi Issa, Hans-Dieter Volk, Michael Schmueck-Henneresse, Petra Reinke, Dimitrios L. Wagner
Publicado en: Molecular Therapy, 2025, ISSN 1525-0016
Editor: Nature Publishing Group
DOI: 10.1016/j.ymthe.2025.01.045

Regulatory T cell therapy is associated with distinct immune regulatory lymphocytic infiltrates in kidney transplants

Autores: Oliver McCallion, Amy R. Cross, Matthew O. Brook, Conor Hennessy, Ricardo Ferreira, Dominik Trzupek, William R. Mulley, Sandeep Kumar, Maria Soares, Ian S. Roberts, Peter J. Friend, Giovanna Lombardi, Kathryn J. Wood, Paul N. Harden, Joanna Hester, Fadi Issa
Publicado en: Med, 2024, Página(s) 100561, ISSN 2666-6340
Editor: Cell press
DOI: 10.1016/j.medj.2024.11.014

CTGCT, Centre of Excellence for the Technologies of Gene and Cell Therapy: Collaborative translation of scientific discoveries into advanced treatments for neurological rare genetic diseases and cancer

Autores: Darja Marolt Presen, Duško Lainšček, Jane Kinghorn, Zsolt Sebestyen, Jurgen Kuball, Leila Amini, Petra Reinke, Anke Fuchs, Roman Jerala, Mojca Benčina
Publicado en: Computational and Structural Biotechnology Journal, Edición 27, 2024, Página(s) 10-16, ISSN 2001-0370
Editor: Elsevier
DOI: 10.1016/j.csbj.2024.11.051

Integration of <i>ζ</i>-deficient CARs into the <i>CD3</i> <i>ζ</i> gene conveys potent cytotoxicity in T and NK cells

Autores: Jonas Kath, Clemens Franke, Vanessa Drosdek, Weijie Du, Viktor Glaser, Carla Fuster-Garcia, Maik Stein, Tatiana Zittel, Sarah Schulenberg, Caroline E. Porter, Lena Andersch, Annette Künkele, Joshua Alcaniz, Jens Hoffmann, Hinrich Abken, Mohamed Abou-el-Enein, Axel Pruß, Masataka Suzuki, Toni Cathomen, Renata Stripecke, Hans-Dieter Volk, Petra Reinke, Michael Schmueck-Henneresse, Dimitrios L. Wag
Publicado en: Blood, Edición 143, 2024, Página(s) 2599-2611, ISSN 0006-4971
Editor: American Society of Hematology
DOI: 10.1182/blood.2023020973

Recent advances in our understanding of the allograft response

Autores: Conor Hennessy , Guido Lewik , Amy Cross , Joanna Hester , Fadi Issa
Publicado en: Faculty Opinions, Edición 10:21, 2021, ISSN 2634-0836
Editor: Faculty opinions
DOI: 10.12703/r/10-21

Toward an Optimized Process for Clinical Manufacturing of CAR-Treg Cell Therapy

Autores: Enrico Fritsche, Hans-Dieter Volk, Petra Reinke, Mohamed Abou-El-Enein
Publicado en: Trends in Biotechnology, 2020, Página(s) 1-13, ISSN 0167-7799
Editor: Elsevier BV
DOI: 10.1016/j.tibtech.2019.12.009

Future prospects for CD8 + regulatory T cells in immune tolerance

Autores: Léa Flippe, Séverine Bézie, Ignacio Anegon, Carole Guillonneau
Publicado en: Immunological Reviews, Edición 292/1, 2019, Página(s) 209-224, ISSN 0105-2896
Editor: Blackwell Publishing Inc.
DOI: 10.1111/imr.12812

Post-marketing safety and efficacy surveillance of cell and gene therapies in the EU: A critical review

Autores: Enrico Fritsche, Magdi Elsallab, Michaela Schaden, Spencer Phillips Hey, Mohamed Abou-El-Enein
Publicado en: Cell and Gene Therapy Insights, Edición 5/11, 2019, Página(s) 1505-1521, ISSN 2059-7800
Editor: BIOINSIGHTS PUBLISHING
DOI: 10.18609/cgti.2019.156

In Vivo Analysis of Human Immune Responses in Immunodeficient Rats

Autores: Séverine Ménoret, Laure-Hélène Ouisse, Laurent Tesson, Séverine Remy, Claire Usal, Aude Guiffes, Vanessa Chenouard, Pierre-Joseph Royer, Gwenaelle Evanno, Bernard Vanhove, Eliane Piaggio, Ignacio Anegon
Publicado en: Transplantation, Edición 104/4, 2020, Página(s) 715-723, ISSN 0041-1337
Editor: Lippincott Williams & Wilkins Ltd.
DOI: 10.1097/tp.0000000000003047

Humanization of Immunodeficient Animals for the Modeling of Transplantation, Graft Versus Host Disease and Regenerative Medicine

Autores: George Adigbli, Séverine Ménoret, Amy R. Cross, Joanna Hester, Fadi Issa, Ignacio Anegon
Publicado en: Transplantation, Edición Publish Ahead of Print, 2020, ISSN 0041-1337
Editor: Lippincott Williams & Wilkins Ltd.
DOI: 10.1097/tp.0000000000003177

New frontiers: cellular immunotherapy beyond cancer

Autores: Matthew Brook, Joanna Hester, Fadi Issa
Publicado en: Cell and Gene Therapy Insights, Edición 5/12, 2019, Página(s) 1229-1236, ISSN 2059-7800
Editor: BIOINSIGHTS
DOI: 10.18609/cgti.2019.163

Non-viral TRAC-knocked-in CD19KICAR-T and gp350KICAR-T cells tested against Burkitt lymphomas with type 1 or 2 EBV infection: In vivo cellular dynamics and potency

Autores: Tobias Braun, Alina Pruene, Milita Darguzyte, Alexander F. vom Stein, Phuong-Hien Nguyen, Dimitrios L. Wagner, Jonas Kath, Alicia Roig-Merino, Michael Heuser, Lucas L. Riehm, Andreas Schneider, Sabine Awerkiew, Steven R. Talbot, André Bleich, Constanca Figueiredo, Martin Bornhäuser, Renata Stripecke
Publicado en: Frontiers in Immunology, Edición 14, 2023, ISSN 1664-3224
Editor: Frontiers Media S.A.
DOI: 10.3389/fimmu.2023.1086433

Super-Treg: Toward a New Era of Adoptive Treg Therapy Enabled by Genetic Modifications

Autores: Leila Amini, Jenny Greig, Michael Schmueck-Henneresse, Hans-Dieter Volk, Séverine Bézie, Petra Reinke, Carole Guillonneau, Dimitrios L Wagner, Ignacio Anegon
Publicado en: Frontiers in Immunology, Edición 611638, 2021, ISSN 1664-3224
Editor: Frontiers
DOI: 10.3389/fimmu.2020.611638

Cas9-directed immune tolerance in humans-a model to evaluate regulatory T cells in gene therapy?

Autores: Dimitrios Laurin Wagner, Lena Peter, Michael Schmueck-Henneresse
Publicado en: Gene Therapy, 2021, Página(s) 549–559, ISSN 0969-7128
Editor: Nature Publishing Group
DOI: 10.1038/s41434-021-00232-2

Chimeric Antigen Receptors and Regulatory T Cells: The Potential for HLA-Specific Immunosuppression in Transplantation

Autores: Sabrina Wright, Conor Hennessy,Joanna Hester, Fadi Issa
Publicado en: Engineering, 2021, ISSN 2095-8099
Editor: Elsevier Ltd
DOI: 10.1016/j.eng.2021.10.018

Gene editing of<i>CD3 epsilon</i>gene to redirect regulatory T cells for adoptive T cell transfer

Autores: Weijie Du, Fatih Noyan, Oliver McCallion, Vanessa Drosdek, Jonas Kath, Viktor Glaser, Carla Fuster-Garcia, Mingxing Yang, Maik Stein, Olaf Weber, Julia K. Polansky, Toni Cathomen, Elmar Jaeckel, Joanna Hester, Fadi Issa, Hans-Dieter Volk, Michael Schmueck-Henneresse, Petra Reinke, Dimitrios L. Wagner
Publicado en: Biorxiv, 2024, ISSN 2692-8205
Editor: cold spring harbor Laboratory
DOI: 10.1101/2024.03.18.584896

Genetic engineering of human and mouse CD4+ and CD8+ Tregs using lentiviral vectors encoding chimeric antigen receptors

Autores: Nadège Vimond, Juliette Lasselin, Ignacio Anegon, Carole Guillonneau, Séverine Bézie
Publicado en: Molecular Therapy - Methods & Clinical Development, Edición 20, 2020, Página(s) 69-85, ISSN 2329-0501
Editor: Elsevier
DOI: 10.1016/j.omtm.2020.11.008

Treg-specific IL-2 therapy can reestablish intrahepatic immune regulation in autoimmune hepatitis

Autores: Laura Elisa Buitrago-Molina, Julia Pietrek, Fatih Noyan, Jerome Schlue, Michael P. Manns, Heiner Wedemeyer, Matthias Hardtke-Wolenski, Elmar Jaeckel
Publicado en: Journal of Autoimmunity, Edición 117, 2021, Página(s) 102591, ISSN 0896-8411
Editor: Academic Press
DOI: 10.1016/j.jaut.2020.102591

Targeted De-Methylation of the FOXP3-TSDR Is Sufficient to Induce Physiological FOXP3 Expression but Not a Functional Treg Phenotype

Autores: Christopher Kressler, Gilles Gasparoni, Karl Nordström, Dania Hamo, Abdulrahman Salhab, Christoforos Dimitropoulos, Sascha Tierling, Petra Reinke, Hans-Dieter Volk, Jörn Walter, Alf Hamann and Julia K. Polansky
Publicado en: Frontiers in Immunology, Edición 609891, 2021, ISSN 1664-3224
Editor: Frontiers
DOI: 10.3389/fimmu.2020.609891

Low-Dose Interleukin-2 Therapy: Fine-tuning Treg in Solid Organ Transplantation?

Autores: Leila Amini, Jaspal Kaeda, Olaf Weber, Petra Reinke
Publicado en: Transplantation, 2024, ISSN 0041-1337
Editor: Lippincott Williams & Wilkins Ltd.
DOI: 10.1097/tp.0000000000004866

Identification, selection, and expansion of non-gene modified alloantigen-reactive Tregs for clinical therapeutic use

Autores: Alaa Alzhrani , Matthew Bottomley , Kathryn Wood , Joanna Hester , Fadi Issa
Publicado en: Cellular Immunology, Edición 357:104214, 2020, ISSN 1090-2163
Editor: Elsevier
DOI: 10.1016/j.cellimm.2020.104214

Non-peer reviewed articles (2)

TCAIM controls effector T cell generation by preventing Mitochondria-Endoplasmic Reticulum Contact Site-initiated Cholesterol Biosynthesis

Autores: Christina Iwert, Julia Stein, Christine Appelt, Katrin Vogt, Roman Josef Rainer, Katja Tummler, Kerstin Mühle, Katarina Stanko, Julia Schumann, Doreen Uebe, Karsten Jürchott, Jan Lisec, Katharina Janek, Christoph Gille, Kathrin Textoris-Taube, Somesh Sai, Ansgar Petersen, Anja A. Kühl, Edda Klipp, Christian Meisel, Birgit Sawitzki
Publicado en: bioRxiv, 2021, ISSN 2692-8205
Editor: Cold Sping Harbor Laboratory
DOI: 10.1101/2021.04.20.440500

Fast, efficient and virus-free generation of TRAC-replaced CAR T cells

Autores: Jonas Kath, Weijie Du, Bernice Thommandru, Rolf Turk, Leila Amini, Maik Stein, Tatiana Zittel, Stefania Martini, Lennard Ostendorf, Andreas Wilhelm, Levent Akyüz, Armin Rehm, Uta E. Höpken, Axel Pruß, Annette Künkele, Ashley M. Jacobi, Hans-Dieter Volk, Michael SchmueckHenneresse, Petra Reinke, Dimitrios L. Wagner
Publicado en: bioRxiv, 2021, ISSN 2692-8205
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.02.14.431017