Precision Docking of Very Large DNA Cargos in Mammalian Genomes

Información del proyecto

DNA-DOCK

Identificador del acuerdo de subvención: 834631

DOI

10.3030/834631

Proyecto cerrado

Fecha de la firma de la CE 22 Mayo 2019

Fecha de inicio 1 Septiembre 2019

Fecha de finalización 28 Febrero 2026

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 2 498 578,00

Aportación de la UE

€ 2 498 578,00

Coordinado por

UNIVERSITY OF BRISTOL
United Kingdom

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (14)

Bottom-up assembly of viral replication cycles

Autores: Oskar Staufer, Gösta Gantner, Ilia Platzman, Klaus Tanner, Imre Berger, Joachim P. Spatz
Publicado en: Nature Communications, Edición 13, 2022, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-022-33661-7

Coenzyme Q10 protects keratinocytes against oxidation-induced energy stress as revealed by spatiotemporal analysis of cell energetics

Autores: Roland Abi Nahed, Ali Hussein, Cécile Cottet-Rousselle, Alexandra Vogelsang, Francesco Aulicino, Imre Berger, Thomas Blatt, Julia M. Weise, Uwe Schlattner
Publicado en: Scientific Reports, Edición 15, 2025, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-025-98793-4

Tuning VSV-G Expression Improves Baculovirus Integrity, Stability and Mammalian Cell Transduction Efficiency

Autores: Martina Mattioli, Renata A. Raele, Gunjan Gautam, Ufuk Borucu, Christiane Schaffitzel, Francesco Aulicino, Imre Berger
Publicado en: Viruses, Edición 16, 2025, Página(s) 1475, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v16091475

Genome editing and kidney health

Autores: Nadia Tavakolidakhrabadi, Francesco Aulicino, Carl J May, Moin A Saleem, Imre Berger, Gavin I Welsh
Publicado en: Clinical Kidney Journal, Edición 17, 2024, ISSN 2048-8505
Editor: Oxford University Press
DOI: 10.1093/ckj/sfae119

Engineering the ADDobody protein scaffold for generation of high-avidity ADDomer super-binders

Autores: Dora Buzas, Huan Sun, Christine Toelzer, Sathish K.N. Yadav, Ufuk Borucu, Gunjan Gautam, Kapil Gupta, Joshua C. Bufton, Julien Capin, Richard B. Sessions, Frederic Garzoni, Imre Berger, Christiane Schaffitzel
Publicado en: Structure, Edición 32, 2026, Página(s) 342-351.e6, ISSN 0969-2126
Editor: Cell Press
DOI: 10.1016/j.str.2023.12.010

An engineered baculoviral protein and DNA co-delivery system for CRISPR-based mammalian genome editing

Autores: Julien Capin; Alexandra Harrison; Renata A Raele; Sathish K N Yadav; Dominique Baiwir; Gabriel Mazzucchelli; Loic Quinton; Timothy J Satchwell; Ashley M Toye; Christiane Schaffitzel; Imre Berger; Francesco Aulicino
Publicado en: Nucleic Acids Research, Edición 52(6), 2024, Página(s) 3450–3468, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkae142

Unlocking the effective alliance of β-lapachone and hydroxytyrosol against triple-negative breast cancer cells

Autores: Jesús Calahorra, José L. Blaya-Cánovas, Olivia Castellini-Pérez, Ernesto Aparicio-Puerta, Candela Cives-Losada, Jose J.G. Marin, Markel Rementeria, Francisca E. Cara, Araceli López-Tejada, Carmen Griñán-Lisón, Francesco Aulicino, Imre Berger, Juan A. Marchal, Violeta Delgado-Almenta, Sergio Granados-Principal
Publicado en: Biomedicine & Pharmacotherapy, Edición 174, 2026, Página(s) 116439, ISSN 0753-3322
Editor: Elsevier Masson
DOI: 10.1016/j.biopha.2024.116439

Microfluidic production and characterization of biofunctionalized giant unilamellar vesicles for targeted intracellular cargo delivery

Autores: Oskar Staufer, Silvia Antona, Dennis Zhang, Júlia Csatári, Martin Schröter, Jan-Willi Janiesch, Sebastian Fabritz, Imre Berger, Ilia Platzman, Joachim P. Spatz
Publicado en: Biomaterials, Edición 264, 2025, Página(s) 120203, ISSN 0142-9612
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2020.120203

The MultiBac BEVS: Basics, applications, performance and recent developments

Autores: Barbara Gorda, Christine Toelzer, Francesco Aulicino, Imre Berger
Publicado en: Methods in Enzymology, Edición 00766879, 2021, Página(s) 129-154, ISSN 0076-6879
Editor: Academic Press
DOI: 10.1016/bs.mie.2021.06.018

The free fatty acid–binding pocket is a conserved hallmark in pathogenic β-coronavirus spike proteins from SARS-CoV to Omicron

Autores: Christine Toelzer, Kapil Gupta, Sathish K. N. Yadav, Lorna Hodgson, Maia Kavanagh Williamson, Dora Buzas, Ufuk Borucu, Kyle Powers, Richard Stenner, Kate Vasileiou, Frederic Garzoni, Daniel Fitzgerald, Christine Payré, Gunjan Gautam, Gérard Lambeau, Andrew D. Davidson, Paul Verkade, Martin Frank, Imre Berger, Christiane Schaffitzel
Publicado en: Science Advances, Edición 8, 2024, ISSN 2375-2548
Editor: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.adc9179

Synthetic Virus-Derived Nanosystems (SVNs) for Delivery and Precision Docking of Large Multifunctional DNA Circuitry in Mammalian Cells

Autores: Francesco Aulicino, Julien Capin, Imre Berger
Publicado en: Pharmaceutics, Edición 12/8, 2020, Página(s) 759, ISSN 1999-4923
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics12080759

Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus

Autores: Francesco Aulicino; Martin Pelosse; Christine Toelzer; Julien Capin; Erwin Ilegems; Parisa Meysami; Ruth Rollarson; Per-Olof Berggren; Mark Simon Dillingham; Christiane Schaffitzel; Moin A Saleem; Gavin I Welsh; Imre Berger
Publicado en: Nucleic Acids Research, Edición 50/13, 2022, Página(s) 7783–7799, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkac587

Cryo-EM reveals binding of linoleic acid to SARS-CoV-2 spike glycoprotein, suggesting an antiviral treatment strategy

Autores: Christine Toelzer, Kapil Gupta, Imre Berger, Christiane Schaffitzel
Publicado en: Acta Crystallographica Section D Structural Biology, Edición 79, 2025, Página(s) 111-121, ISSN 2059-7983
Editor: International Union of Crystallography (IUCr)
DOI: 10.1107/s2059798323000049

<i>In vitro</i> generated antibodies guide thermostable ADDomer nanoparticle design for nasal vaccination and passive immunization against SARS-CoV-2

Autores: Dora Buzas; Adrian H Bunzel; Oskar Staufer; Emily J Milodowski; Grace L Edmunds; Joshua C Bufton; Beatriz V Vidana Mateo; Sathish K N Yadav; Kapil Gupta; Charlotte Fletcher; Maia K Williamson; Alexandra Harrison; Ufuk Borucu; Julien Capin; Ore Francis; Georgia Balchin; Sophie Hall; Mirella V Vega; Fabien Durbesson; Srikanth Lingappa; Renaud Vincentelli; Joe Roe; Linda Wooldridge; Rachel Burt; Ross
Publicado en: Antibody Therapeutics, Edición 6, 2023, Página(s) 277–297, ISSN 2516-4236
Editor: Oxford University Press
DOI: 10.1093/abt/tbad024

Thesis and dissertations (3)

SynBac: minimal synthetic baculovirus genomes

Autores: Barbara Gorda
Publicado en: 2022, Página(s) Entire Thesis
Editor: University of Bristol

A Baculoviral ‘Swag Bag’ Protein and DNA Delivery Toolkit for CRISPR-based Editing of Human Genomes

Autores: Julien Capin
Publicado en: 2022, Página(s) Entire Thesis
Editor: University of Bristol

Harnessing Baculoviruses for Gene Therapy: A Protein Delivery ApproachAlexandra Harrison

Autores: Alexandra Harrison
Publicado en: 2024
Editor: University of Bristol

Book chapters (2)

FRET-Based Sensor for Measuring Adenine Nucleotide Binding to AMPK

Autores: Roland Abi Nahed, Martin Pelosse, Francesco Aulicino, Florine Cottaz, Imre Berger, Uwe Schlattner
Publicado en: Methods in Molecular Biology, Nutrient Sensing in Eukaryotes, 2025, Página(s) 15-45
Editor: Springer US
DOI: 10.1007/978-1-0716-4284-9_2

Assembly of Baculovirus Vectors for Multiplexed Prime Editing

Autores: Francesco Aulicino, Renata A. Raele, Alexandra Harrison, Imre Berger
Publicado en: Methods in Molecular Biology, Baculovirus, 2024, Página(s) 301-327
Editor: Springer US
DOI: 10.1007/978-1-0716-3961-0_24

Other (2)

Antibodies generated <i>in vitro</i> and <i>in vivo</i> elucidate design of a thermostable ADDomer COVID-19 nasal nanoparticle vaccine

Autores: Dora Buzas, H Adrian Bunzel, Oskar Staufer, Emily J Milodowski, Grace L Edmunds, Joshua C Bufton, Beatriz V Vidana Mateo, Sathish K N Yadav, Kapil Gupta, Charlotte Fletcher, Maia Kavanagh Williamson, Alexandra Harrison, Ufuk Borucu, Julien Capin, Ore Francis, Georgia Balchin, Sophie Hall, Mirella Vivoli Vega, Fabien Durbesson, Renaud Vincentelli, Joe Roe, Linda Wooldridge, Rachel Burt, J L Ross An
Publicado en: 2026
Editor: openRxiv
DOI: 10.1101/2023.03.17.533092

Baculovirus-vectored precision delivery of large DNA cargoes in human genomes

Autores: Francesco Aulicino, Martin Pelosse, Christine Toelzer, Julien Capin, Parisa Meysami, Mark Simon Dillingham, Christiane Schaffitzel, Imre Berger
Publicado en: 2026
Editor: openRxiv
DOI: 10.1101/2020.08.17.253898