Precision Docking of Very Large DNA Cargos in Mammalian Genomes

Informazioni relative al progetto

DNA-DOCK

ID dell’accordo di sovvenzione: 834631

DOI

10.3030/834631

Progetto chiuso

Data della firma CE 22 Maggio 2019

Data di avvio 1 Settembre 2019

Data di completamento 28 Febbraio 2026

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 2 498 578,00

Contributo UE

€ 2 498 578,00

Coordinato da

UNIVERSITY OF BRISTOL
United Kingdom

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (14)

Bottom-up assembly of viral replication cycles

Autori: Oskar Staufer, Gösta Gantner, Ilia Platzman, Klaus Tanner, Imre Berger, Joachim P. Spatz
Pubblicato in: Nature Communications, Numero 13, 2022, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-33661-7

Coenzyme Q10 protects keratinocytes against oxidation-induced energy stress as revealed by spatiotemporal analysis of cell energetics

Autori: Roland Abi Nahed, Ali Hussein, Cécile Cottet-Rousselle, Alexandra Vogelsang, Francesco Aulicino, Imre Berger, Thomas Blatt, Julia M. Weise, Uwe Schlattner
Pubblicato in: Scientific Reports, Numero 15, 2025, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-025-98793-4

Tuning VSV-G Expression Improves Baculovirus Integrity, Stability and Mammalian Cell Transduction Efficiency

Autori: Martina Mattioli, Renata A. Raele, Gunjan Gautam, Ufuk Borucu, Christiane Schaffitzel, Francesco Aulicino, Imre Berger
Pubblicato in: Viruses, Numero 16, 2025, Pagina/e 1475, ISSN 1999-4915
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v16091475

Genome editing and kidney health

Autori: Nadia Tavakolidakhrabadi, Francesco Aulicino, Carl J May, Moin A Saleem, Imre Berger, Gavin I Welsh
Pubblicato in: Clinical Kidney Journal, Numero 17, 2024, ISSN 2048-8505
Editore: Oxford University Press
DOI: 10.1093/ckj/sfae119

Engineering the ADDobody protein scaffold for generation of high-avidity ADDomer super-binders

Autori: Dora Buzas, Huan Sun, Christine Toelzer, Sathish K.N. Yadav, Ufuk Borucu, Gunjan Gautam, Kapil Gupta, Joshua C. Bufton, Julien Capin, Richard B. Sessions, Frederic Garzoni, Imre Berger, Christiane Schaffitzel
Pubblicato in: Structure, Numero 32, 2026, Pagina/e 342-351.e6, ISSN 0969-2126
Editore: Cell Press
DOI: 10.1016/j.str.2023.12.010

An engineered baculoviral protein and DNA co-delivery system for CRISPR-based mammalian genome editing

Autori: Julien Capin; Alexandra Harrison; Renata A Raele; Sathish K N Yadav; Dominique Baiwir; Gabriel Mazzucchelli; Loic Quinton; Timothy J Satchwell; Ashley M Toye; Christiane Schaffitzel; Imre Berger; Francesco Aulicino
Pubblicato in: Nucleic Acids Research, Numero 52(6), 2024, Pagina/e 3450–3468, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkae142

Unlocking the effective alliance of β-lapachone and hydroxytyrosol against triple-negative breast cancer cells

Autori: Jesús Calahorra, José L. Blaya-Cánovas, Olivia Castellini-Pérez, Ernesto Aparicio-Puerta, Candela Cives-Losada, Jose J.G. Marin, Markel Rementeria, Francisca E. Cara, Araceli López-Tejada, Carmen Griñán-Lisón, Francesco Aulicino, Imre Berger, Juan A. Marchal, Violeta Delgado-Almenta, Sergio Granados-Principal
Pubblicato in: Biomedicine & Pharmacotherapy, Numero 174, 2026, Pagina/e 116439, ISSN 0753-3322
Editore: Elsevier Masson
DOI: 10.1016/j.biopha.2024.116439

Microfluidic production and characterization of biofunctionalized giant unilamellar vesicles for targeted intracellular cargo delivery

Autori: Oskar Staufer, Silvia Antona, Dennis Zhang, Júlia Csatári, Martin Schröter, Jan-Willi Janiesch, Sebastian Fabritz, Imre Berger, Ilia Platzman, Joachim P. Spatz
Pubblicato in: Biomaterials, Numero 264, 2025, Pagina/e 120203, ISSN 0142-9612
Editore: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2020.120203

The MultiBac BEVS: Basics, applications, performance and recent developments

Autori: Barbara Gorda, Christine Toelzer, Francesco Aulicino, Imre Berger
Pubblicato in: Methods in Enzymology, Numero 00766879, 2021, Pagina/e 129-154, ISSN 0076-6879
Editore: Academic Press
DOI: 10.1016/bs.mie.2021.06.018

The free fatty acid–binding pocket is a conserved hallmark in pathogenic β-coronavirus spike proteins from SARS-CoV to Omicron

Autori: Christine Toelzer, Kapil Gupta, Sathish K. N. Yadav, Lorna Hodgson, Maia Kavanagh Williamson, Dora Buzas, Ufuk Borucu, Kyle Powers, Richard Stenner, Kate Vasileiou, Frederic Garzoni, Daniel Fitzgerald, Christine Payré, Gunjan Gautam, Gérard Lambeau, Andrew D. Davidson, Paul Verkade, Martin Frank, Imre Berger, Christiane Schaffitzel
Pubblicato in: Science Advances, Numero 8, 2024, ISSN 2375-2548
Editore: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.adc9179

Synthetic Virus-Derived Nanosystems (SVNs) for Delivery and Precision Docking of Large Multifunctional DNA Circuitry in Mammalian Cells

Autori: Francesco Aulicino, Julien Capin, Imre Berger
Pubblicato in: Pharmaceutics, Numero 12/8, 2020, Pagina/e 759, ISSN 1999-4923
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics12080759

Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus

Autori: Francesco Aulicino; Martin Pelosse; Christine Toelzer; Julien Capin; Erwin Ilegems; Parisa Meysami; Ruth Rollarson; Per-Olof Berggren; Mark Simon Dillingham; Christiane Schaffitzel; Moin A Saleem; Gavin I Welsh; Imre Berger
Pubblicato in: Nucleic Acids Research, Numero 50/13, 2022, Pagina/e 7783–7799, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkac587

Cryo-EM reveals binding of linoleic acid to SARS-CoV-2 spike glycoprotein, suggesting an antiviral treatment strategy

Autori: Christine Toelzer, Kapil Gupta, Imre Berger, Christiane Schaffitzel
Pubblicato in: Acta Crystallographica Section D Structural Biology, Numero 79, 2025, Pagina/e 111-121, ISSN 2059-7983
Editore: International Union of Crystallography (IUCr)
DOI: 10.1107/s2059798323000049

<i>In vitro</i> generated antibodies guide thermostable ADDomer nanoparticle design for nasal vaccination and passive immunization against SARS-CoV-2

Autori: Dora Buzas; Adrian H Bunzel; Oskar Staufer; Emily J Milodowski; Grace L Edmunds; Joshua C Bufton; Beatriz V Vidana Mateo; Sathish K N Yadav; Kapil Gupta; Charlotte Fletcher; Maia K Williamson; Alexandra Harrison; Ufuk Borucu; Julien Capin; Ore Francis; Georgia Balchin; Sophie Hall; Mirella V Vega; Fabien Durbesson; Srikanth Lingappa; Renaud Vincentelli; Joe Roe; Linda Wooldridge; Rachel Burt; Ross
Pubblicato in: Antibody Therapeutics, Numero 6, 2023, Pagina/e 277–297, ISSN 2516-4236
Editore: Oxford University Press
DOI: 10.1093/abt/tbad024

Thesis and dissertations (3)

SynBac: minimal synthetic baculovirus genomes

Autori: Barbara Gorda
Pubblicato in: 2022, Pagina/e Entire Thesis
Editore: University of Bristol

A Baculoviral ‘Swag Bag’ Protein and DNA Delivery Toolkit for CRISPR-based Editing of Human Genomes

Autori: Julien Capin
Pubblicato in: 2022, Pagina/e Entire Thesis
Editore: University of Bristol

Harnessing Baculoviruses for Gene Therapy: A Protein Delivery ApproachAlexandra Harrison

Autori: Alexandra Harrison
Pubblicato in: 2024
Editore: University of Bristol

Book chapters (2)

FRET-Based Sensor for Measuring Adenine Nucleotide Binding to AMPK

Autori: Roland Abi Nahed, Martin Pelosse, Francesco Aulicino, Florine Cottaz, Imre Berger, Uwe Schlattner
Pubblicato in: Methods in Molecular Biology, Nutrient Sensing in Eukaryotes, 2025, Pagina/e 15-45
Editore: Springer US
DOI: 10.1007/978-1-0716-4284-9_2

Assembly of Baculovirus Vectors for Multiplexed Prime Editing

Autori: Francesco Aulicino, Renata A. Raele, Alexandra Harrison, Imre Berger
Pubblicato in: Methods in Molecular Biology, Baculovirus, 2024, Pagina/e 301-327
Editore: Springer US
DOI: 10.1007/978-1-0716-3961-0_24

Other (2)

Antibodies generated <i>in vitro</i> and <i>in vivo</i> elucidate design of a thermostable ADDomer COVID-19 nasal nanoparticle vaccine

Autori: Dora Buzas, H Adrian Bunzel, Oskar Staufer, Emily J Milodowski, Grace L Edmunds, Joshua C Bufton, Beatriz V Vidana Mateo, Sathish K N Yadav, Kapil Gupta, Charlotte Fletcher, Maia Kavanagh Williamson, Alexandra Harrison, Ufuk Borucu, Julien Capin, Ore Francis, Georgia Balchin, Sophie Hall, Mirella Vivoli Vega, Fabien Durbesson, Renaud Vincentelli, Joe Roe, Linda Wooldridge, Rachel Burt, J L Ross An
Pubblicato in: 2026
Editore: openRxiv
DOI: 10.1101/2023.03.17.533092

Baculovirus-vectored precision delivery of large DNA cargoes in human genomes

Autori: Francesco Aulicino, Martin Pelosse, Christine Toelzer, Julien Capin, Parisa Meysami, Mark Simon Dillingham, Christiane Schaffitzel, Imre Berger
Pubblicato in: 2026
Editore: openRxiv
DOI: 10.1101/2020.08.17.253898