Description du projet
La nanotechnologie en ADN pour une meilleure gestion du diabète
L’évolution rapide des technologies de détection requiert des biocapteurs capables d’assurer une surveillance continue grâce à un processus en une seule étape. Les performances des biocapteurs disponibles sont entravées par la force de liaison de l’unité de reconnaissance moléculaire, qui limite la plage dynamique du capteur et est souvent liée à la sortie du signal. Le projet GlucOrigami, financé par l’UE, propose de découpler les unités de reconnaissance moléculaire et de transmission des signaux cellulaires du biocapteur à l’aide de nanostructures d’origami ADN auto-assemblées et programmables. La solution sera présentée en utilisant comme modèle un biocapteur de glucose pour la surveillance de la maladie chez les patients diabétiques, l’origami ADN servant à positionner avec précision tous les éléments du biocapteur: une paire de multi-fluorophores comme unité de transmission des signaux cellulaires et d’amplification du signal, et des protéines de liaison au glucose/galactose comme unité de reconnaissance moléculaire.
Objectif
Biosensors play a crucial role in our everyday lives from health monitoring to disease detection. The rapid advancement of sensing technologies dictates an ever growing need for improved biosensors which are capable to continuously monitor analytes in a single-step process and yield low-cost devices. The performance of many biosensors is, however, limited by the binding strength of their molecular recognition unit which dictates the dynamic range of the sensor and is often tightly connected to the signal transduction unit, i.e. its signal output. In this project, I propose to globally solve this limitation by decoupling the molecular recognition and signal transduction units of the biosensor by exploiting self-assembled and programmable DNA origami nanostructures. This fundamental approach will be demonstrated by the design of a sensitive and tunable biosensor for glucose, whose sensing is of utmost importance for the disease monitoring of diabetic patients. DNA origami will be utilized to precisely position all biosensor elements: multifluorophore FRET pair, which will serve as a signal transduction and amplification unit as well as glucose/galactose binding proteins and glucose functionalities, which will provide a molecular recognition unit. Different biomimicry strategies to tune the useful dynamic range of the biosensors will be evaluated aiming to achieve sensitivity at a physiologically relevant glucose concentration. Finally, the potential to combine these advanced glucose biosensors with low-cost read-out instruments (such as smartphone cameras) will be assessed. The DNA origami glucose sensor proposed here is of great promise for the development of wearable and low-cost glucose sensing devices for diabetes monitoring, which will grow into a large demand in our society. Moreover, it will allow me to merge DNA nanotechnology, molecular biology, spectroscopy and chemistry research, laying the foundations upon which to build my future career in Europe.
Champ scientifique
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensors
- natural sciencesbiological sciencesgeneticsDNA
- medical and health sciencesclinical medicineendocrinologydiabetes
- natural sciencesphysical sciencesopticsspectroscopy
- natural sciencesbiological sciencesmolecular biology
Programme(s)
Régime de financement
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinateur
80539 MUNCHEN
Allemagne