Descripción del proyecto
Del veneno a los fármacos: la venómica evolutiva posibilita el desarrollo de sustancias terapéuticas nuevas
Muchos animales han desarrollado adaptaciones para producir veneno que, en esencia, es una secreción bioquímica que sirve para la defensa, la depredación y la competencia. Los diferentes venenos, a pesar de su complejidad, presentan compuestos y dianas comunes. El objetivo del proyecto financiado con fondos europeos Finding VENomS es estudiar la evolución de toxinas y venenos, de los cuales se sabe muy poco. Sus investigadores combinarán técnicas transcriptómicas y proteómicas para determinar la composición del veneno en los nemertinos, un filo de gusanos eucelomados no segmentados. El trabajo del proyecto no solo proporcionará información sobre la evolución del veneno, sino que además podría determinar nuevos compuestos bioactivos con aplicaciones en la industria farmacéutica como medicamentos precursores o herramientas biotecnológicas.
Objetivo
Animal venoms are key adaptations that have evolved independently in many taxa to assist in defence, predation and competition. Venoms are some of the most complex biochemical secretions known in nature, but despite this complexity, there is a high degree of convergence in toxin structure and targets, making venomous organisms great model systems to investigate areas as diverse as molecular evolution, functional convergence and drug discovery. However, the processes underlying toxin and venom evolution remain poorly understood, particularly in invertebrates. With recent advancements in sequencing and analytical techniques these neglected taxa are being increasingly investigated, revealing a high genetic and functional diversity of venom compounds and challenging traditional views about venom evolution. Still, many phyla such as ribbon worms (Nemertea), active predators that use toxins for defense and predation, remain understudied. This project aims to investigate venom evolution in Nemertea using an integrative evolutionary venomics approach. I propose to use a transcriptomics-proteomics approach referred to as proteogenomics, combining RNA-seq differential gene expression analysis (DGE) and tandem mass spectrometry-based proteomics (MS/MS) to determine venom composition, and integrate these data with expression and functional morphology data derived from spatial omics, both spatial transcriptomics (ST) and spatial proteomics (MALDI-IMS), transmission and scanning electron microscopy (TEM and SEM). This will advance our understanding of ribbon worm venom systems, and shed new light into the true diversity of animal venoms and their evolution. Additionally, this research will likely uncover novel bioactive compounds, with great potential as drug leads and biotechnological tools, making this project’s findings highly relevant to the H2020 focus area Blue Growth objective of developing new bio-based products, including pharmaceuticals.
Programa(s)
Régimen de financiación
MSCA-IF-EF-ST - Standard EFCoordinador
28006 Madrid
España