CORDIS - EU research results
CORDIS

Bio-Inspired Glycan-NanoProbes as Antimicrobial Pro-Drugs

Project description

Glycan-based nanoprobes for delivering antibiotics into bacteria

Cell surface carbohydrates play key roles in bacterial adhesion. These interactions exhibit high specificity and weak affinities towards their carbohydrate ligands, compensated by the host presenting the carbohydrate ligands in a multivalent manner or as clusters on the cell or mucosal surface. A disaccharide can act as a carrier on bi-functionalised fluorescent nanoparticles to help the intracellular delivery of other non-internalisable glycan moieties, avoiding the endosomal/lysosomal degradation pathway. Recently developed water-soluble, non-toxic fluorescent carbon-based nanomaterials have shown that they are capable of labelling both gram-negative and gram-positive bacteria. The EU-funded BioNanoProbes project aims to develop a new class of glycan-based nanoprobes for labelling and delivery of antibiotics into bacteria.

Objective

Methods for specific recognition and targeting of bacteria are of key importance in developing approaches to counter the growth of antimicrobial resistance (AMR). Cell surface carbohydrates play key roles in cell recognition mechanisms and bacterial adhesion. These key interactions typically exhibit high specificity and weak affinities toward their carbohydrate ligand. This low affinity is compensated in nature by the architecture of the protein, the host presenting the carbohydrate ligands in a multivalent manner or as clusters on the cell or mucosal surface. Glyco-nanomaterials offer the possibility of attaching several different molecules to the same nanoparticle while controlling the relative densities of these ligands. Recently, the Galan group demonstrated that a simple disaccharide, such as lactose can act as a “Trojan horse” on bi-functionalized fluorescent nanopartiples (CdSe QDs) to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway. Following this, the group has developed a new class of water-soluble, non-toxic fluorescent carbon-based nanomaterials which are easily accessible from cheap carbohydrate starting materials and more excitingly, preliminary data have shown that these new carbon nanodots are able to label both Gram-negative and Gram-positive bacteria. Based on these exciting results, the aim of this project is to develop a new class of glycan-based nanoprobes for labelling and delivery of antibiotics into bacteria. The glycan-based nano pro-drugs will be evaluated in bacterial binding and killing assays and screened for selective labelling and drug release using confocal microscopy and TEM. This is a multidisciplinary project involving synthetic organic and materials chemistry, glycobiology and microbiology.

Coordinator

UNIVERSITY OF BRISTOL
Net EU contribution
€ 212 933,76
Address
BEACON HOUSE QUEENS ROAD
BS8 1QU Bristol
United Kingdom

See on map

Region
South West (England) Gloucestershire, Wiltshire and Bristol/Bath area Bristol, City of
Activity type
Higher or Secondary Education Establishments
Links
Total cost
€ 212 933,76