Descripción del proyecto
El primer estudio básico y aplicado de la autofagia en la fresa
La autofagia es el principal mecanismo de resistencia contra el estrés biótico y abiótico en diferentes especies de plantas. Sin embargo, se sabe poco sobre el efecto de la inducción a la autofagia en la respuesta de las plantas al estrés. Esta cuestión adquiere mayor importancia en el contexto del calentamiento global, dado que la producción de cultivos con mayor resistencia al calor y a la sequía se hace imprescindible. En el proyecto FrATGaria, financiado con fondos europeos, se estudiará el papel de la autofagia en la maduración de una de las bayas más importantes de la Unión Europea: la fresa («Fragaria x ananassa»). El equipo del proyecto generará plantas transgénicas con aumento de la autofagia y se centrará en analizar los posibles beneficios en cuanto al desarrollo general, la calidad del fruto y la resistencia al estrés que pueden tener estas plantas en comparación con las silvestres.
Objetivo
"Autophagy, the major catabolic pathway in eukaryotic cells, has a role in the resistance to biotic and abiotic stresses in different plant species. Plenty of studies show that blocking autophagy by knocking out some of its essential genes leads to plants highly susceptible to this stresses (including heat and drought). However, there are much less studies about the effect of autophagy induction on the response of plants to stresses. Present situation of global warming is already having an effect in agriculture due to a rise in temperature and to water shortages. Therefore, generation of crops with higher resistance to heat and drought should be a priority nowadays. In this proposal I will study the role of autophagy in the maturation of one of the most important berry fruit in EU, Strawberry (Fragaria x ananassa). I will determine the importance of this catabolic process in the response to heat/drought stress in this crop. Furthermore, I will use the diploid strawberry model specie Fragaria vesca to generate transgenic plants with increased autophagy by CRISPR/Cas9 and CRISPR/dCas9 genome editing and analyze the potential benefits regarding general development, fruit quality and stress resistance (this previously shown for Arabidopsis, tomato and apple) that this plants can have compare to wild type. This will be the first basic and applied study of autophagy in strawberry. ""FrATGaria"" will combine my experience in the autophagy field with the expertise of the host group in strawberry development and fruit ripening and will benefit from active collaboration with strawberry breeding groups. The development of this project will allow me to broaden my knowledge about plant development in response to stress strengthening not only my scientific skills but also my leadership, project managment and mentoring capabilities."
Ámbito científico
Programa(s)
Régimen de financiación
MSCA-IF-EF-ST - Standard EFCoordinador
29016 Malaga
España