Descrizione del progetto
Far progredire la simbiosi batterica in agricoltura
La fissazione dell’azoto nelle piante di legumi è un ottimo esempio di simbiosi mutualistica con batteri rizobi. Il progetto HARNESS, finanziato dall’UE, sta studiando l’evoluzione e il funzionamento di queste simbiosi imitandone l’evoluzione a livello sperimentale. Gli esperimenti sono stati progettati per testimoniare in tempo reale in che modo i batteri si adattano alla loro nuova pianta ospite e la transizione alla simbiosi. I ricercatori eseguiranno anche analisi genetiche per individuare i fattori determinanti delle funzioni batteriche che sono alla base di questa simbiosi, nonché le potenziali mutazioni che migliorano l’adattamento batterico. Nel complesso, queste informazioni contribuiranno a estendere l’applicazione della simbiosi a vari ecosistemi agricoli.
Obiettivo
Microbiota shape growth and survival of eukaryotes through countless symbiotic associations. A prominent example for agriculture is the mutualistic nitrogen-fixing symbiosis between legume plants and rhizobial bacteria. Understanding the evolution and functioning of these symbioses offers promises to optimise their beneficial use in agro-ecosystems and, potentially, to extend it to non-legume plants. An ambitious project initiated several years ago in the host team aims at recapulating the evolution of new nitrogen-fixing bacteria from a pathogenic ancestor. A symbiotic plasmid was artificially transferred into the plant pathogen Ralstonia solancearum and the resulting chimera was selected for improved in planta symbiotic performance by experimental evolution. This experiment offers a unique opportunity to witness the ‘real-time’ adaptation of chimeric bacteria to their new host plant. In this proposal, I will leverage the biological material generated during this experiment to progress towards an integrative understanding of the evolutionary events underpinning the transition to symbiosis. In particular, I will focus on the acquisition of intracellular uptake and accommodation of bacteria by plant cells, a defining and poorly understood aspect of these symbiotic associations. First, I will use whole-population sequencing to track allelic frequencies in evolving populations and identify mutations improving bacterial intracellular fitness. Functional genetics analyses will then uncover the bacterial functions that are required for endosymbiotic life. Finally, plant transcriptional responses to bacteria showing different infectious abilities will be analysed by RNA-sequencing. Altogether, this work will use a combination of approaches (experimental evolution, genetics and high-throughput sequencing) to advance our understanding of the genetic and selective processes underpinning the evolution of nitrogen-fixing symbioses.
Campo scientifico
CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.
CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.
- natural sciencesbiological sciencesmicrobiologybacteriology
- natural sciencesbiological sciencesevolutionary biology
- natural sciencesbiological sciencesgeneticsmutation
- natural sciencesbiological sciencesbiological behavioural sciencesethologybiological interactions
- natural sciencesbiological sciencesgeneticsRNA
Parole chiave
Programma(i)
Argomento(i)
Meccanismo di finanziamento
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinatore
75007 Paris
Francia