Obiettivo Mesenchymal stem cells (MSC) isolated from the Wharton jelly of the umbilical cord (UC-MSC) show great potential for the use in allogeneic stem cell therapies for various human diseases. However, the major limitation for the clinical use of allogeneic UC-MSC is their procoagulation activity, which is a consequence of expression of the tissue factor (TF), that can cause thromboembolism after transplantation, thus limiting their clinical usefulness. Preparation of UC-MSC without the TF (UCMSC-TF-) would enable safe clinical use of UC-MSC and bring their full therapeutic potential for treatment of patients. For this reason, preparation of UCMSC-TF- is the central goal of the “APApore4RNA” project. In the project, UCMSC-TF- will be prepared by utilizing the small interfering RNA (siRNA) directed against TF to silence the expression of TF. The project will develop a novel nanotechnology for ex vivo intracellular delivery of siRNA that will combine the use of biodegradable calcium hydroxyapatite (APA) nanoparticles (NPs) (fellow’s experience) and electroporation (EP) (experience of the company EDUCELL) to enable efficient, safe and reproducible transfection of UC-MSC. The use of APA NPs will be based on the fellow’s recent study published in Biomaterials that revealed their unique membrane-disruptive property that enables rapid permeation of payloads across plasma membrane. Moreover, as APA has low dielectric constant, it is expected that APA NPs will strongly improve the EP process by reducing the applied electric field required for reversible membrane permeabilization and consequently improve the safety and efficacy of EP. In addition, important goal of the “APApore4RNA” project is to gain fundamental knowledge about underlying mechanisms of APA-mediated electropermeabilization of plasma membrane and payload permeation across the membrane. Campo scientifico scienze naturaliscienze chimichechimica inorganicametalli alcalino terrosiscienze mediche e della salutebiotecnologia medicatecnologie cellularicellule staminaliscienze mediche e della salutemedicina clinicatrapiantoingegneria e tecnologiabiotecnologia industrialebiomaterialescienze naturaliscienze chimicheelettrochimicabioelettrochimicaelettroporazione Parole chiave Calcium phosphates Mesenchymal stem cells RNAi siRNA Therapy Intracellular Delivery Nanoparticles Programma(i) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Argomento(i) MSCA-IF-2018 - Individual Fellowships Invito a presentare proposte H2020-MSCA-IF-2018 Vedi altri progetti per questo bando Meccanismo di finanziamento MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinatore EDUCELL PODJETJE ZA CELICNO BIOLOGI JO DOO Contribution nette de l'UE € 162 040,32 Indirizzo Prevale 9 1236 Trzin Slovenia Mostra sulla mappa PMI L’organizzazione si è definita una PMI (piccola e media impresa) al momento della firma dell’accordo di sovvenzione. Sì Regione Slovenija Zahodna Slovenija Osrednjeslovenska Tipo di attività Private for-profit entities (excluding Higher or Secondary Education Establishments) Collegamenti Contatta l’organizzazione Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Altri finanziamenti € 0,00
