Monoclonal Antibodies with Binding Sensitive To Environmental Regulation

Información del proyecto

MABSTER

Identificador del acuerdo de subvención: 850974

DOI

10.3030/850974

Proyecto cerrado

Fecha de la firma de la CE 8 Octubre 2019

Fecha de inicio 1 Enero 2020

Fecha de finalización 31 Diciembre 2024

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 1 500 000,00

Aportación de la UE

€ 1 500 000,00

Coordinado por

DANMARKS TEKNISKE UNIVERSITET
Denmark

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (33)

Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes

Autores: Jack Wade, Charlotte Rimbault, Hanif Ali, Line Ledsgaard, Esperanza Rivera-de-Torre, Maher Abou Hachem, Kim Boddum, Nadia Mirza, Markus-Frederik Bohn, Siri A. Sakya, Fulgencio Ruso-Julve, Jan Terje Andersen, Andreas H. Laustsen
Publicado en: Bioconjugate Chemistry, 2022, ISSN 1043-1802
Editor: American Chemical Society
DOI: 10.1021/acs.bioconjchem.2c00220

The rise of genomics in snake venom research: recent advances and future perspectives

Autores: Wei-qiao Rao, Konstantinos Kalogeropoulos, Morten E Allentoft, Shyam Gopalakrishnan, Wei-ning Zhao, Christopher T. Workman, Cecilie Knudsen, Belén Jiménez-Mena, Lorenzo Seneci, Mahsa Mousavi-Derazmahalleh, Timothy P Jenkins, Esperanza Rivera-de-Torre, Si-qi Liu, Andreas H. Laustsen
Publicado en: GigaScience, 2022, ISSN 2047-217X
Editor: Oxford Academic
DOI: 10.1093/gigascience/giac024

Causes and Consequences of Snake Venom Variation

Autores: Nicholas R. Casewell, Timothy N.W. Jackson, Andreas H. Laustsen, Kartik Sunagar
Publicado en: Trends in Pharmacological Sciences, 2020, ISSN 2423-3722
Editor: Elsevier
DOI: 10.1016/j.tips.2020.05.006

ExpoSeq: simplified analysis of high-throughput sequencing data from antibody discovery campaigns

Autores: Christoffer V Sørensen, Nils Hofmann, Puneet Rawat, Frederik V Sørensen, Anne Ljungars, Victor Greiff, Andreas H Laustsen, Timothy P Jenkins
Publicado en: Bioinformatics Advances, Edición 4, 2024, ISSN 2635-0041
Editor: Oxford Academic
DOI: 10.1093/bioadv/vbae020

Neutralization capacity of recombinant antivenoms based on monoclonal antibodies and nanobodies

Autores: Anne Ljungars, Andreas H. Laustsen
Publicado en: Toxicon, 2022, Página(s) 106991, ISSN 0041-0101
Editor: Pergamon Press
DOI: 10.1016/j.toxicon.2022.106991

Antibody-dependent enhancement of toxicity of myotoxin II from Bothrops asper

Autores: Christoffer V. Sørensen, Julián Fernández, Anna Christina Adams, Helen H. K. Wildenauer, Sanne Schoffelen, Line Ledsgaard, Manuela B. Pucca, Michael Fiebig, Felipe A. Cerni, Tulika Tulika, Bjørn G. Voldborg, Aneesh Karatt-Vellatt, J. Preben Morth, Anne Ljungars, Lise M. Grav, Bruno Lomonte, Andreas H. Laustsen
Publicado en: Nature Communications, Edición 15, 2024, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-023-42624-5

Characterisation of two snake toxin-targeting human monoclonal immunoglobulin G antibodies expressed in tobacco plants

Autores: Catherine M. Moore, Anne Ljungars, Matthew J. Paul, Camilla Holst Dahl, Shirin Ahmadi, Anna Christina Adams, Lise Marie Grav, Sanne Schoffelen, Bjørn Gunnar Voldborg, Andreas Hougaard Laustsen, Julian K-C Ma
Publicado en: Toxicon, Edición 232, 2024, Página(s) 107225, ISSN 0041-0101
Editor: Pergamon Press
DOI: 10.1016/j.toxicon.2023.107225

V-ToCs (Venom Toxin Clustering): A tool for the investigation of sequence and structure similarities in snake venom toxins

Autores: Konstantinos Kalogeropoulos, Vlad Rosca, Carol O'Brien, Charlotte Risager Christensen, Rahmat Grahadi, Christoffer Vinther Sørensen, Max D. Overath, Diego Ruiz Espi, David E. Jenkins, Ulrich auf dem Keller, Andreas H. Laustsen, Thomas J. Fryer, Timothy P. Jenkins
Publicado en: Toxicon, Edición 250, 2024, Página(s) 108088, ISSN 0041-0101
Editor: Pergamon Press
DOI: 10.1016/j.toxicon.2024.108088

Synthetic antibodies block receptor binding and current‐inhibiting effects of α‐cobratoxin from <i>Naja kaouthia</i>

Autores: Shane Miersch; Guillermo de la Rosa; Rasmus Friis; Line Ledsgaard; Kim Boddum; Andreas H. Laustsen; Sachdev S. Sidhu
Publicado en: Protein Science, 2022, ISSN 1469-896X
Editor: Wiley
DOI: 10.1002/pro.4296

A Comparative Study of Protein Structure Prediction Tools for Challenging Targets: Snake Venom Toxins

Autores: Konstantinos Kalogeropoulos, Markus-Frederik Bohn, David E. Jenkins, Jann Ledergerber, Christoffer V. Sørensen, Nils Hofmann, Jack Wade, Thomas Fryer, Giang Thi Tuyet Nguyen, Ullrich auf dem Keller, Andreas H. Laustsen, Timothy P. Jenkins
Publicado en: Toxicon, 2024, ISSN 0041-0101
Editor: Pergamon Press
DOI: 10.1101/2023.05.05.539526

In vitro discovery of a human monoclonal antibody that neutralizes lethality of cobra snake venom

Autores: Line Ledsgaard, Andreas H. Laustsen, Urska Pus, Jack Wade, Pedro Villar, Kim Boddum, Peter Slavny, Edward W. Masters, Ana S. Arias, Saioa Oscoz, Daniel T. Griffiths, Alice M. Luther, Majken Lindholm, Rachael A. Leah, Marie Sofie Møller, Hanif Ali, John McCafferty, Bruno Lomonte, José M. Gutiérrez, Aneesh Karatt-Vellatt
Publicado en: mAbs, 2022, ISSN 1942-0862
Editor: Taylor & Francis
DOI: 10.1080/19420862.2022.2085536

Advances in antibody phage display technology

Autores: Line Ledsgaard, AnneLjungars, Charlotte Rimbault, Christoffer V. Sørensen, Tulika Tulika, Jack Wade, Yessica Wouters, John McCafferty, Andreas H. Laustsen
Publicado en: Drug Discovery Today, 2022, ISSN 1359-6446
Editor: Elsevier BV
DOI: 10.1016/j.drudis.2022.05.002

Current Knowledge on Snake Dry Bites

Autores: Manuela B. Pucca, Cecilie Knudsen, Isadora S. Oliveira, Charlotte Rimbault, Felipe A. Cerni, Fan Hui Wen, Jacqueline Sachett, Marco A. Sartim, Andreas H. Laustsen, Wuelton M. Monteiro
Publicado en: Toxins, 2020, ISSN 2072-6651
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins12110668

Discovery of broadly‐neutralizing antibodies against brown recluse spider and Gadim scorpion sphingomyelinases using consensus toxins as antigens

Autores: Esperanza Rivera‐de‐Torre, Stefanos Lampadariou, Mark Møiniche, Markus F. Bohn, Seyed Mahdi Kazemi, Andreas H. Laustsen
Publicado en: Protein Science, Edición 33, 2024, ISSN 0961-8368
Editor: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4901

Phage display assisted discovery of a <scp>pH</scp>‐dependent anti‐α‐cobratoxin antibody from a natural variable domain library

Autores: Tulika Tulika, Rasmus W. Pedersen, Charlotte Rimbault, Shirin Ahmadi, Esperanza Rivera‐de‐Torre, Monica L. Fernández‐Quintero, Johannes R. Loeffler, Markus‐Frederik Bohn, Anne Ljungars, Line Ledsgaard, Bjørn G. Voldborg, Fulgencio Ruso‐Julve, Jan Terje Andersen, Andreas H. Laustsen
Publicado en: Protein Science, Edición 32, 2024, ISSN 0961-8368
Editor: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4821

Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins

Autores: Esperanza Rivera-de-Torre; Charlotte Rimbault; Timothy P. Jenkins; Christoffer V. Sørensen; Anna Damsbo; Natalie J. Saez; Yoan Duhoo; Celeste Menuet Hackney; Lars Ellgaard; Andreas H. Laustsen
Publicado en: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Editor: Frontiers Media
DOI: 10.3389/fbioe.2021.811905

Assessing developability early in the discovery process for novel biologics

Autores: Monica L. Fernández-Quintero, Anne Ljungars, Franz Waibl, Victor Greiff, Jan Terje Andersen, Torleif T. Gjølberg, Timothy P. Jenkins, Bjørn Gunnar Voldborg, Lise Marie Grav, Sandeep Kumar, Guy Georges, Hubert Kettenberger, Klaus R. Liedl, Peter M. Tessier, John McCafferty, Andreas H. Laustsen
Publicado en: mAbs, Edición 15, 2024, ISSN 1942-0870
Editor: Landes Bioscience
DOI: 10.1080/19420862.2023.2171248

Recombinant snake antivenoms get closer to the clinic

Autores: Andreas H. Laustsen
Publicado en: Trends in Immunology, Edición 45, 2024, Página(s) 225-227, ISSN 1471-4906
Editor: Elsevier BV
DOI: 10.1016/j.it.2024.03.001

From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research

Autores: Shirin Ahmadi, Melisa Benard-Valle, Kim Boddum, Fernanda C. Cardoso, Glenn F. King, Andreas Hougaard Laustsen, Anne Ljungars
Publicado en: Frontiers in Pharmacology, Edición 14, 2023, ISSN 1663-9812
Editor: Frontiers Media S.A.
DOI: 10.3389/fphar.2023.1249336

Cross-reactivity trends when selecting scFv antibodies against snake toxins using a phage display-based cross-panning strategy

Autores: Christoffer V. Sørensen, Line Ledsgaard, Helen H. K. Wildenauer, Camilla H. Dahl, Tasja W. Ebersole, Markus-Frederik Bohn, Anne Ljungars, Timothy P. Jenkins, Andreas H. Laustsen
Publicado en: Scientific Reports, Edición 13, 2023, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-023-37056-6

Animal Immunization, in Vitro Display Technologies, and Machine Learning for Antibody Discovery.

Autores: Andreas Hougaard Laustsen; Victor Greiff; Aneesh Karatt-Vellatt; Serge Muyldermans; Timothy P. Jenkins
Publicado en: Trends in Biotechnology, 2021, ISSN 1879-3096
Editor: Elsevier
DOI: 10.1016/j.tibtech.2021.03.003

A single-chain variable fragment selected against a conformational epitope of a recombinantly produced snake toxin using phage display

Autores: Charlotte Rimbault, Pelle D. Knudsen, Anna Damsbo, Kim Boddum, Hanif Ali, Celeste M. Hackney, Lars Ellgaard, Markus-Frederik Bohn, Andreas H. Laustsen
Publicado en: New Biotechnology, Edición 76, 2023, Página(s) 23-32, ISSN 1871-6784
Editor: Elsevier BV
DOI: 10.1016/j.nbt.2023.04.002

The challenges with developing therapeutic monoclonal antibodies for pandemic application

Autores: Andreas H. Laustsen, Markus-Frederik Bohn, Anne Ljungars
Publicado en: Expert Opinion on Drug Discovery, 2021, ISSN 1746-0441
Editor: Informa Healthcare
DOI: 10.1080/17460441.2021.1976141

Structural and functional analyses of nematode-derived antimicrobial peptides support the occurrence of direct mechanisms of worm-microbiota interactions

Autores: James Rooney, Esperanza Rivera-de-Torre, Ruizhe Li, Kevin Mclean, Daniel R.G. Price, Alasdair J. Nisbet, Andreas H. Laustsen, Timothy P. Jenkins, Andreas Hofmann, Somenath Bakshi, Ashraf Zarkan, Cinzia Cantacessi
Publicado en: Computational and Structural Biotechnology Journal, Edición 23, 2024, Página(s) 1522-1533, ISSN 2001-0370
Editor: Elsevier
DOI: 10.1016/j.csbj.2024.04.019

A comparative study of the performance of E. coli and K. phaffii for expressing α-cobratoxin

Autores: Anna Damsbo, Charlotte Rimbault, Nick J. Burlet, Anneline Vlamynck, Ida Bisbo, Selma B. Belfakir, Andreas H. Laustsen, Esperanza Rivera-de-Torre
Publicado en: Toxicon, Edición 239, 2024, Página(s) 107613, ISSN 0041-0101
Editor: Pergamon Press
DOI: 10.1016/j.toxicon.2024.107613

In vivo neutralization of coral snake venoms with an oligoclonal nanobody mixture in a murine challenge model

Autores: Melisa Benard-Valle, Yessica Wouters, Anne Ljungars, Giang Thi Tuyet Nguyen, Shirin Ahmadi, Tasja Wainani Ebersole, Camilla Holst Dahl, Alid Guadarrama-Martínez, Frederikke Jeppesen, Helena Eriksen, Gibran Rodríguez-Barrera, Kim Boddum, Timothy Patrick Jenkins, Sara Petersen Bjørn, Sanne Schoffelen, Bjørn Gunnar Voldborg, Alejandro Alagón, Andreas Hougaard Laustsen
Publicado en: Nature Communications, Edición 15, 2024, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-024-48539-z

Engineering of pH-dependent antigen binding properties for toxin-targeting IgG1 antibodies using light-chain shuffling

Autores: Tulika Tulika, Fulgencio Ruso-Julve, Shirin Ahmadi, Anne Ljungars, Esperanza Rivera-de-Torre, Jack Wade, Monica L. Fernández-Quintero, Timothy P. Jenkins, Selma B. Belfakir, Georgina M.S. Ross, Lars Boyens-Thiele, Alexander K. Buell, Siri A. Sakya, Christoffer V. Sørensen, Markus-Frederik Bohn, Line Ledsgaard, Bjørn G. Voldborg, Chiara Francavilla, Tilman Schlothauer, Bruno Lomonte, Jan Terje A
Publicado en: Structure, Edición 32, 2024, Página(s) 1404-1418.e7, ISSN 0969-2126
Editor: Cell Press
DOI: 10.1016/j.str.2024.07.014

AHA: AI-guided tool for the quantification of venom-induced haemorrhage in mice

Autores: Timothy P. Jenkins1, William Michael Laprade, Andrés Sánchez, Tulika Tulika, Carol O’Brien, Christoffer V. Sørensen, Trenton K. Stewart, Thomas Fryer, Andreas H. Laustsen, José María Gutiérrez
Publicado en: Frontiers in Tropical Diseases, 2022, ISSN 2673-7515
Editor: Frontiers
DOI: 10.3389/fitd.2022.1063640

De novo designed proteins neutralize lethal snake venom toxins

Autores: David Baker, Susana Vazquez Torres, Melisa Benard Valle, Stephen Mackessy, Stefanie Menzies, Nicholas Casewell, Shirin Ahmadi, Nick Burlet, Edin Muratspahić, Isaac Sappington, Max Overath, Esperanza Rivera-de-Torre, Jann Ledergerber, Andreas Hougaard Laustsen, Kim Boddum, Asim Bera, Alex Kang, Evans Brackenbrough, Iara Cardoso, Edouard Crittenden, Rebecca Edge, Justin Decarreau, Robert Ragotte, A
Publicado en: Nature, 2025, ISSN 1476-4687
Editor: Springer Nature
DOI: 10.21203/rs.3.rs-4402792/v1

Target product profiles for pan-Africa recombinant antivenoms against neurotoxic or hemotoxic and cytotoxic snakebite envenoming

Autores: Andreas H. Laustsen, Melisa Benard-Valle, Abdulrazaq G. Habib, Nicholas R. Casewell, Michael Abouyannis, David G. Lalloo, Anne Ljungars
Publicado en: PLOS Neglected Tropical Diseases, Edición 19, 2025, Página(s) e0012833, ISSN 1935-2735
Editor: Public Library of Sciences
DOI: 10.1371/journal.pntd.0012833

Towards better antivenoms: navigating the road to new types of snakebite envenoming therapies

Autores: Suthimon Thumtecho, Nick J. Burlet, Anne Ljungars, Andreas H. Laustsen
Publicado en: Journal of Venomous Animals and Toxins including Tropical Diseases, Edición 29, 2023, ISSN 1678-9199
Editor: Center for the Study of Venoms and Venomous Animals (CEVAP)
DOI: 10.1590/1678-9199-jvatitd-2023-0057

Discovery and optimization of a broadly-neutralizing human monoclonal antibody against long-chain α-neurotoxins from snakes

Autores: Line Ledsgaard, Jack Wade, Timothy P. Jenkins, Kim Boddum, Irina Oganesyan, Julian A. Harrison, Pedro Villar, Rachael A. Leah, Renato Zenobi, Sanne Schoffelen, Bjørn Voldborg, Anne Ljungars, John McCafferty, Bruno Lomonte, José M. Gutiérrez, Andreas H. Laustsen, Aneesh Karatt-Vellatt
Publicado en: Nature Communications, Edición 14, 2023, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-023-36393-4

Terrestrial venomous animals, the envenomings they cause, and treatment perspectives in the Middle East and North Africa.

Autores: Timothy P. Jenkins; Shirin Ahmadi; Mátyás A. Bittenbinder; Mátyás A. Bittenbinder; Trenton K Stewart; Dilber E Akgun; Melissa Hale; Nafiseh Nasri Nasrabadi; Nafiseh Nasri Nasrabadi; Darian S Wolff; Freek J. Vonk; Freek J. Vonk; Jeroen Kool; Andreas Hougaard Laustsen
Publicado en: PLOS Neglected Tropical Diseases, 2021, ISSN 1935-2727
Editor: Public Library of Science
DOI: 10.1371/journal.pntd.0009880

Monographic books (1)

Discovery of antibodies with pH-dependent binding towards snake venom toxin

Autores: Ej Efternavn, Tulika
Publicado en: Ej Efternavn , T 2023 , Discovery of antibodies with pH-dependent binding towards snake venom toxin . DTU Bioengineering , Kgs. Lyngby, Denmark ., Edición 1, 2023
Editor: Technical University of Denmark