Monoclonal Antibodies with Binding Sensitive To Environmental Regulation

Informazioni relative al progetto

MABSTER

ID dell’accordo di sovvenzione: 850974

DOI

10.3030/850974

Progetto chiuso

Data della firma CE 8 Ottobre 2019

Data di avvio 1 Gennaio 2020

Data di completamento 31 Dicembre 2024

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 1 500 000,00

Contributo UE

€ 1 500 000,00

Coordinato da

DANMARKS TEKNISKE UNIVERSITET
Denmark

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (33)

Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes

Autori: Jack Wade, Charlotte Rimbault, Hanif Ali, Line Ledsgaard, Esperanza Rivera-de-Torre, Maher Abou Hachem, Kim Boddum, Nadia Mirza, Markus-Frederik Bohn, Siri A. Sakya, Fulgencio Ruso-Julve, Jan Terje Andersen, Andreas H. Laustsen
Pubblicato in: Bioconjugate Chemistry, 2022, ISSN 1043-1802
Editore: American Chemical Society
DOI: 10.1021/acs.bioconjchem.2c00220

The rise of genomics in snake venom research: recent advances and future perspectives

Autori: Wei-qiao Rao, Konstantinos Kalogeropoulos, Morten E Allentoft, Shyam Gopalakrishnan, Wei-ning Zhao, Christopher T. Workman, Cecilie Knudsen, Belén Jiménez-Mena, Lorenzo Seneci, Mahsa Mousavi-Derazmahalleh, Timothy P Jenkins, Esperanza Rivera-de-Torre, Si-qi Liu, Andreas H. Laustsen
Pubblicato in: GigaScience, 2022, ISSN 2047-217X
Editore: Oxford Academic
DOI: 10.1093/gigascience/giac024

Causes and Consequences of Snake Venom Variation

Autori: Nicholas R. Casewell, Timothy N.W. Jackson, Andreas H. Laustsen, Kartik Sunagar
Pubblicato in: Trends in Pharmacological Sciences, 2020, ISSN 2423-3722
Editore: Elsevier
DOI: 10.1016/j.tips.2020.05.006

ExpoSeq: simplified analysis of high-throughput sequencing data from antibody discovery campaigns

Autori: Christoffer V Sørensen, Nils Hofmann, Puneet Rawat, Frederik V Sørensen, Anne Ljungars, Victor Greiff, Andreas H Laustsen, Timothy P Jenkins
Pubblicato in: Bioinformatics Advances, Numero 4, 2024, ISSN 2635-0041
Editore: Oxford Academic
DOI: 10.1093/bioadv/vbae020

Neutralization capacity of recombinant antivenoms based on monoclonal antibodies and nanobodies

Autori: Anne Ljungars, Andreas H. Laustsen
Pubblicato in: Toxicon, 2022, Pagina/e 106991, ISSN 0041-0101
Editore: Pergamon Press
DOI: 10.1016/j.toxicon.2022.106991

Antibody-dependent enhancement of toxicity of myotoxin II from Bothrops asper

Autori: Christoffer V. Sørensen, Julián Fernández, Anna Christina Adams, Helen H. K. Wildenauer, Sanne Schoffelen, Line Ledsgaard, Manuela B. Pucca, Michael Fiebig, Felipe A. Cerni, Tulika Tulika, Bjørn G. Voldborg, Aneesh Karatt-Vellatt, J. Preben Morth, Anne Ljungars, Lise M. Grav, Bruno Lomonte, Andreas H. Laustsen
Pubblicato in: Nature Communications, Numero 15, 2024, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-42624-5

Characterisation of two snake toxin-targeting human monoclonal immunoglobulin G antibodies expressed in tobacco plants

Autori: Catherine M. Moore, Anne Ljungars, Matthew J. Paul, Camilla Holst Dahl, Shirin Ahmadi, Anna Christina Adams, Lise Marie Grav, Sanne Schoffelen, Bjørn Gunnar Voldborg, Andreas Hougaard Laustsen, Julian K-C Ma
Pubblicato in: Toxicon, Numero 232, 2024, Pagina/e 107225, ISSN 0041-0101
Editore: Pergamon Press
DOI: 10.1016/j.toxicon.2023.107225

V-ToCs (Venom Toxin Clustering): A tool for the investigation of sequence and structure similarities in snake venom toxins

Autori: Konstantinos Kalogeropoulos, Vlad Rosca, Carol O'Brien, Charlotte Risager Christensen, Rahmat Grahadi, Christoffer Vinther Sørensen, Max D. Overath, Diego Ruiz Espi, David E. Jenkins, Ulrich auf dem Keller, Andreas H. Laustsen, Thomas J. Fryer, Timothy P. Jenkins
Pubblicato in: Toxicon, Numero 250, 2024, Pagina/e 108088, ISSN 0041-0101
Editore: Pergamon Press
DOI: 10.1016/j.toxicon.2024.108088

Synthetic antibodies block receptor binding and current‐inhibiting effects of α‐cobratoxin from <i>Naja kaouthia</i>

Autori: Shane Miersch; Guillermo de la Rosa; Rasmus Friis; Line Ledsgaard; Kim Boddum; Andreas H. Laustsen; Sachdev S. Sidhu
Pubblicato in: Protein Science, 2022, ISSN 1469-896X
Editore: Wiley
DOI: 10.1002/pro.4296

A Comparative Study of Protein Structure Prediction Tools for Challenging Targets: Snake Venom Toxins

Autori: Konstantinos Kalogeropoulos, Markus-Frederik Bohn, David E. Jenkins, Jann Ledergerber, Christoffer V. Sørensen, Nils Hofmann, Jack Wade, Thomas Fryer, Giang Thi Tuyet Nguyen, Ullrich auf dem Keller, Andreas H. Laustsen, Timothy P. Jenkins
Pubblicato in: Toxicon, 2024, ISSN 0041-0101
Editore: Pergamon Press
DOI: 10.1101/2023.05.05.539526

In vitro discovery of a human monoclonal antibody that neutralizes lethality of cobra snake venom

Autori: Line Ledsgaard, Andreas H. Laustsen, Urska Pus, Jack Wade, Pedro Villar, Kim Boddum, Peter Slavny, Edward W. Masters, Ana S. Arias, Saioa Oscoz, Daniel T. Griffiths, Alice M. Luther, Majken Lindholm, Rachael A. Leah, Marie Sofie Møller, Hanif Ali, John McCafferty, Bruno Lomonte, José M. Gutiérrez, Aneesh Karatt-Vellatt
Pubblicato in: mAbs, 2022, ISSN 1942-0862
Editore: Taylor & Francis
DOI: 10.1080/19420862.2022.2085536

Advances in antibody phage display technology

Autori: Line Ledsgaard, AnneLjungars, Charlotte Rimbault, Christoffer V. Sørensen, Tulika Tulika, Jack Wade, Yessica Wouters, John McCafferty, Andreas H. Laustsen
Pubblicato in: Drug Discovery Today, 2022, ISSN 1359-6446
Editore: Elsevier BV
DOI: 10.1016/j.drudis.2022.05.002

Current Knowledge on Snake Dry Bites

Autori: Manuela B. Pucca, Cecilie Knudsen, Isadora S. Oliveira, Charlotte Rimbault, Felipe A. Cerni, Fan Hui Wen, Jacqueline Sachett, Marco A. Sartim, Andreas H. Laustsen, Wuelton M. Monteiro
Pubblicato in: Toxins, 2020, ISSN 2072-6651
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins12110668

Discovery of broadly‐neutralizing antibodies against brown recluse spider and Gadim scorpion sphingomyelinases using consensus toxins as antigens

Autori: Esperanza Rivera‐de‐Torre, Stefanos Lampadariou, Mark Møiniche, Markus F. Bohn, Seyed Mahdi Kazemi, Andreas H. Laustsen
Pubblicato in: Protein Science, Numero 33, 2024, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4901

Phage display assisted discovery of a <scp>pH</scp>‐dependent anti‐α‐cobratoxin antibody from a natural variable domain library

Autori: Tulika Tulika, Rasmus W. Pedersen, Charlotte Rimbault, Shirin Ahmadi, Esperanza Rivera‐de‐Torre, Monica L. Fernández‐Quintero, Johannes R. Loeffler, Markus‐Frederik Bohn, Anne Ljungars, Line Ledsgaard, Bjørn G. Voldborg, Fulgencio Ruso‐Julve, Jan Terje Andersen, Andreas H. Laustsen
Pubblicato in: Protein Science, Numero 32, 2024, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4821

Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins

Autori: Esperanza Rivera-de-Torre; Charlotte Rimbault; Timothy P. Jenkins; Christoffer V. Sørensen; Anna Damsbo; Natalie J. Saez; Yoan Duhoo; Celeste Menuet Hackney; Lars Ellgaard; Andreas H. Laustsen
Pubblicato in: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Editore: Frontiers Media
DOI: 10.3389/fbioe.2021.811905

Assessing developability early in the discovery process for novel biologics

Autori: Monica L. Fernández-Quintero, Anne Ljungars, Franz Waibl, Victor Greiff, Jan Terje Andersen, Torleif T. Gjølberg, Timothy P. Jenkins, Bjørn Gunnar Voldborg, Lise Marie Grav, Sandeep Kumar, Guy Georges, Hubert Kettenberger, Klaus R. Liedl, Peter M. Tessier, John McCafferty, Andreas H. Laustsen
Pubblicato in: mAbs, Numero 15, 2024, ISSN 1942-0870
Editore: Landes Bioscience
DOI: 10.1080/19420862.2023.2171248

Recombinant snake antivenoms get closer to the clinic

Autori: Andreas H. Laustsen
Pubblicato in: Trends in Immunology, Numero 45, 2024, Pagina/e 225-227, ISSN 1471-4906
Editore: Elsevier BV
DOI: 10.1016/j.it.2024.03.001

From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research

Autori: Shirin Ahmadi, Melisa Benard-Valle, Kim Boddum, Fernanda C. Cardoso, Glenn F. King, Andreas Hougaard Laustsen, Anne Ljungars
Pubblicato in: Frontiers in Pharmacology, Numero 14, 2023, ISSN 1663-9812
Editore: Frontiers Media S.A.
DOI: 10.3389/fphar.2023.1249336

Cross-reactivity trends when selecting scFv antibodies against snake toxins using a phage display-based cross-panning strategy

Autori: Christoffer V. Sørensen, Line Ledsgaard, Helen H. K. Wildenauer, Camilla H. Dahl, Tasja W. Ebersole, Markus-Frederik Bohn, Anne Ljungars, Timothy P. Jenkins, Andreas H. Laustsen
Pubblicato in: Scientific Reports, Numero 13, 2023, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-023-37056-6

Animal Immunization, in Vitro Display Technologies, and Machine Learning for Antibody Discovery.

Autori: Andreas Hougaard Laustsen; Victor Greiff; Aneesh Karatt-Vellatt; Serge Muyldermans; Timothy P. Jenkins
Pubblicato in: Trends in Biotechnology, 2021, ISSN 1879-3096
Editore: Elsevier
DOI: 10.1016/j.tibtech.2021.03.003

A single-chain variable fragment selected against a conformational epitope of a recombinantly produced snake toxin using phage display

Autori: Charlotte Rimbault, Pelle D. Knudsen, Anna Damsbo, Kim Boddum, Hanif Ali, Celeste M. Hackney, Lars Ellgaard, Markus-Frederik Bohn, Andreas H. Laustsen
Pubblicato in: New Biotechnology, Numero 76, 2023, Pagina/e 23-32, ISSN 1871-6784
Editore: Elsevier BV
DOI: 10.1016/j.nbt.2023.04.002

The challenges with developing therapeutic monoclonal antibodies for pandemic application

Autori: Andreas H. Laustsen, Markus-Frederik Bohn, Anne Ljungars
Pubblicato in: Expert Opinion on Drug Discovery, 2021, ISSN 1746-0441
Editore: Informa Healthcare
DOI: 10.1080/17460441.2021.1976141

Structural and functional analyses of nematode-derived antimicrobial peptides support the occurrence of direct mechanisms of worm-microbiota interactions

Autori: James Rooney, Esperanza Rivera-de-Torre, Ruizhe Li, Kevin Mclean, Daniel R.G. Price, Alasdair J. Nisbet, Andreas H. Laustsen, Timothy P. Jenkins, Andreas Hofmann, Somenath Bakshi, Ashraf Zarkan, Cinzia Cantacessi
Pubblicato in: Computational and Structural Biotechnology Journal, Numero 23, 2024, Pagina/e 1522-1533, ISSN 2001-0370
Editore: Elsevier
DOI: 10.1016/j.csbj.2024.04.019

A comparative study of the performance of E. coli and K. phaffii for expressing α-cobratoxin

Autori: Anna Damsbo, Charlotte Rimbault, Nick J. Burlet, Anneline Vlamynck, Ida Bisbo, Selma B. Belfakir, Andreas H. Laustsen, Esperanza Rivera-de-Torre
Pubblicato in: Toxicon, Numero 239, 2024, Pagina/e 107613, ISSN 0041-0101
Editore: Pergamon Press
DOI: 10.1016/j.toxicon.2024.107613

In vivo neutralization of coral snake venoms with an oligoclonal nanobody mixture in a murine challenge model

Autori: Melisa Benard-Valle, Yessica Wouters, Anne Ljungars, Giang Thi Tuyet Nguyen, Shirin Ahmadi, Tasja Wainani Ebersole, Camilla Holst Dahl, Alid Guadarrama-Martínez, Frederikke Jeppesen, Helena Eriksen, Gibran Rodríguez-Barrera, Kim Boddum, Timothy Patrick Jenkins, Sara Petersen Bjørn, Sanne Schoffelen, Bjørn Gunnar Voldborg, Alejandro Alagón, Andreas Hougaard Laustsen
Pubblicato in: Nature Communications, Numero 15, 2024, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-024-48539-z

Engineering of pH-dependent antigen binding properties for toxin-targeting IgG1 antibodies using light-chain shuffling

Autori: Tulika Tulika, Fulgencio Ruso-Julve, Shirin Ahmadi, Anne Ljungars, Esperanza Rivera-de-Torre, Jack Wade, Monica L. Fernández-Quintero, Timothy P. Jenkins, Selma B. Belfakir, Georgina M.S. Ross, Lars Boyens-Thiele, Alexander K. Buell, Siri A. Sakya, Christoffer V. Sørensen, Markus-Frederik Bohn, Line Ledsgaard, Bjørn G. Voldborg, Chiara Francavilla, Tilman Schlothauer, Bruno Lomonte, Jan Terje A
Pubblicato in: Structure, Numero 32, 2024, Pagina/e 1404-1418.e7, ISSN 0969-2126
Editore: Cell Press
DOI: 10.1016/j.str.2024.07.014

AHA: AI-guided tool for the quantification of venom-induced haemorrhage in mice

Autori: Timothy P. Jenkins1, William Michael Laprade, Andrés Sánchez, Tulika Tulika, Carol O’Brien, Christoffer V. Sørensen, Trenton K. Stewart, Thomas Fryer, Andreas H. Laustsen, José María Gutiérrez
Pubblicato in: Frontiers in Tropical Diseases, 2022, ISSN 2673-7515
Editore: Frontiers
DOI: 10.3389/fitd.2022.1063640

De novo designed proteins neutralize lethal snake venom toxins

Autori: David Baker, Susana Vazquez Torres, Melisa Benard Valle, Stephen Mackessy, Stefanie Menzies, Nicholas Casewell, Shirin Ahmadi, Nick Burlet, Edin Muratspahić, Isaac Sappington, Max Overath, Esperanza Rivera-de-Torre, Jann Ledergerber, Andreas Hougaard Laustsen, Kim Boddum, Asim Bera, Alex Kang, Evans Brackenbrough, Iara Cardoso, Edouard Crittenden, Rebecca Edge, Justin Decarreau, Robert Ragotte, A
Pubblicato in: Nature, 2025, ISSN 1476-4687
Editore: Springer Nature
DOI: 10.21203/rs.3.rs-4402792/v1

Target product profiles for pan-Africa recombinant antivenoms against neurotoxic or hemotoxic and cytotoxic snakebite envenoming

Autori: Andreas H. Laustsen, Melisa Benard-Valle, Abdulrazaq G. Habib, Nicholas R. Casewell, Michael Abouyannis, David G. Lalloo, Anne Ljungars
Pubblicato in: PLOS Neglected Tropical Diseases, Numero 19, 2025, Pagina/e e0012833, ISSN 1935-2735
Editore: Public Library of Sciences
DOI: 10.1371/journal.pntd.0012833

Towards better antivenoms: navigating the road to new types of snakebite envenoming therapies

Autori: Suthimon Thumtecho, Nick J. Burlet, Anne Ljungars, Andreas H. Laustsen
Pubblicato in: Journal of Venomous Animals and Toxins including Tropical Diseases, Numero 29, 2023, ISSN 1678-9199
Editore: Center for the Study of Venoms and Venomous Animals (CEVAP)
DOI: 10.1590/1678-9199-jvatitd-2023-0057

Discovery and optimization of a broadly-neutralizing human monoclonal antibody against long-chain α-neurotoxins from snakes

Autori: Line Ledsgaard, Jack Wade, Timothy P. Jenkins, Kim Boddum, Irina Oganesyan, Julian A. Harrison, Pedro Villar, Rachael A. Leah, Renato Zenobi, Sanne Schoffelen, Bjørn Voldborg, Anne Ljungars, John McCafferty, Bruno Lomonte, José M. Gutiérrez, Andreas H. Laustsen, Aneesh Karatt-Vellatt
Pubblicato in: Nature Communications, Numero 14, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-36393-4

Terrestrial venomous animals, the envenomings they cause, and treatment perspectives in the Middle East and North Africa.

Autori: Timothy P. Jenkins; Shirin Ahmadi; Mátyás A. Bittenbinder; Mátyás A. Bittenbinder; Trenton K Stewart; Dilber E Akgun; Melissa Hale; Nafiseh Nasri Nasrabadi; Nafiseh Nasri Nasrabadi; Darian S Wolff; Freek J. Vonk; Freek J. Vonk; Jeroen Kool; Andreas Hougaard Laustsen
Pubblicato in: PLOS Neglected Tropical Diseases, 2021, ISSN 1935-2727
Editore: Public Library of Science
DOI: 10.1371/journal.pntd.0009880

Monographic books (1)

Discovery of antibodies with pH-dependent binding towards snake venom toxin

Autori: Ej Efternavn, Tulika
Pubblicato in: Ej Efternavn , T 2023 , Discovery of antibodies with pH-dependent binding towards snake venom toxin . DTU Bioengineering , Kgs. Lyngby, Denmark ., Numero 1, 2023
Editore: Technical University of Denmark