Monoclonal Antibodies with Binding Sensitive To Environmental Regulation

Informations projet

MABSTER

N° de convention de subvention: 850974

DOI

10.3030/850974

Projet clôturé

Date de signature de la CE 8 Octobre 2019

Date de début 1 Janvier 2020

Date de fin 31 Decembre 2024

Financé au titre de

• EXCELLENT SCIENCE - European Research Council (ERC)

Coût total

€ 1 500 000,00

Contribution de l’UE

€ 1 500 000,00

Coordonné par

DANMARKS TEKNISKE UNIVERSITET
Denmark

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Publications

Peer reviewed articles (33)

Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes

Auteurs: Jack Wade, Charlotte Rimbault, Hanif Ali, Line Ledsgaard, Esperanza Rivera-de-Torre, Maher Abou Hachem, Kim Boddum, Nadia Mirza, Markus-Frederik Bohn, Siri A. Sakya, Fulgencio Ruso-Julve, Jan Terje Andersen, Andreas H. Laustsen
Publié dans: Bioconjugate Chemistry, 2022, ISSN 1043-1802
Éditeur: American Chemical Society
DOI: 10.1021/acs.bioconjchem.2c00220

The rise of genomics in snake venom research: recent advances and future perspectives

Auteurs: Wei-qiao Rao, Konstantinos Kalogeropoulos, Morten E Allentoft, Shyam Gopalakrishnan, Wei-ning Zhao, Christopher T. Workman, Cecilie Knudsen, Belén Jiménez-Mena, Lorenzo Seneci, Mahsa Mousavi-Derazmahalleh, Timothy P Jenkins, Esperanza Rivera-de-Torre, Si-qi Liu, Andreas H. Laustsen
Publié dans: GigaScience, 2022, ISSN 2047-217X
Éditeur: Oxford Academic
DOI: 10.1093/gigascience/giac024

Causes and Consequences of Snake Venom Variation

Auteurs: Nicholas R. Casewell, Timothy N.W. Jackson, Andreas H. Laustsen, Kartik Sunagar
Publié dans: Trends in Pharmacological Sciences, 2020, ISSN 2423-3722
Éditeur: Elsevier
DOI: 10.1016/j.tips.2020.05.006

ExpoSeq: simplified analysis of high-throughput sequencing data from antibody discovery campaigns

Auteurs: Christoffer V Sørensen, Nils Hofmann, Puneet Rawat, Frederik V Sørensen, Anne Ljungars, Victor Greiff, Andreas H Laustsen, Timothy P Jenkins
Publié dans: Bioinformatics Advances, Numéro 4, 2024, ISSN 2635-0041
Éditeur: Oxford Academic
DOI: 10.1093/bioadv/vbae020

Neutralization capacity of recombinant antivenoms based on monoclonal antibodies and nanobodies

Auteurs: Anne Ljungars, Andreas H. Laustsen
Publié dans: Toxicon, 2022, Page(s) 106991, ISSN 0041-0101
Éditeur: Pergamon Press
DOI: 10.1016/j.toxicon.2022.106991

Antibody-dependent enhancement of toxicity of myotoxin II from Bothrops asper

Auteurs: Christoffer V. Sørensen, Julián Fernández, Anna Christina Adams, Helen H. K. Wildenauer, Sanne Schoffelen, Line Ledsgaard, Manuela B. Pucca, Michael Fiebig, Felipe A. Cerni, Tulika Tulika, Bjørn G. Voldborg, Aneesh Karatt-Vellatt, J. Preben Morth, Anne Ljungars, Lise M. Grav, Bruno Lomonte, Andreas H. Laustsen
Publié dans: Nature Communications, Numéro 15, 2024, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-023-42624-5

Characterisation of two snake toxin-targeting human monoclonal immunoglobulin G antibodies expressed in tobacco plants

Auteurs: Catherine M. Moore, Anne Ljungars, Matthew J. Paul, Camilla Holst Dahl, Shirin Ahmadi, Anna Christina Adams, Lise Marie Grav, Sanne Schoffelen, Bjørn Gunnar Voldborg, Andreas Hougaard Laustsen, Julian K-C Ma
Publié dans: Toxicon, Numéro 232, 2024, Page(s) 107225, ISSN 0041-0101
Éditeur: Pergamon Press
DOI: 10.1016/j.toxicon.2023.107225

V-ToCs (Venom Toxin Clustering): A tool for the investigation of sequence and structure similarities in snake venom toxins

Auteurs: Konstantinos Kalogeropoulos, Vlad Rosca, Carol O'Brien, Charlotte Risager Christensen, Rahmat Grahadi, Christoffer Vinther Sørensen, Max D. Overath, Diego Ruiz Espi, David E. Jenkins, Ulrich auf dem Keller, Andreas H. Laustsen, Thomas J. Fryer, Timothy P. Jenkins
Publié dans: Toxicon, Numéro 250, 2024, Page(s) 108088, ISSN 0041-0101
Éditeur: Pergamon Press
DOI: 10.1016/j.toxicon.2024.108088

Synthetic antibodies block receptor binding and current‐inhibiting effects of α‐cobratoxin from <i>Naja kaouthia</i>

Auteurs: Shane Miersch; Guillermo de la Rosa; Rasmus Friis; Line Ledsgaard; Kim Boddum; Andreas H. Laustsen; Sachdev S. Sidhu
Publié dans: Protein Science, 2022, ISSN 1469-896X
Éditeur: Wiley
DOI: 10.1002/pro.4296

A Comparative Study of Protein Structure Prediction Tools for Challenging Targets: Snake Venom Toxins

Auteurs: Konstantinos Kalogeropoulos, Markus-Frederik Bohn, David E. Jenkins, Jann Ledergerber, Christoffer V. Sørensen, Nils Hofmann, Jack Wade, Thomas Fryer, Giang Thi Tuyet Nguyen, Ullrich auf dem Keller, Andreas H. Laustsen, Timothy P. Jenkins
Publié dans: Toxicon, 2024, ISSN 0041-0101
Éditeur: Pergamon Press
DOI: 10.1101/2023.05.05.539526

In vitro discovery of a human monoclonal antibody that neutralizes lethality of cobra snake venom

Auteurs: Line Ledsgaard, Andreas H. Laustsen, Urska Pus, Jack Wade, Pedro Villar, Kim Boddum, Peter Slavny, Edward W. Masters, Ana S. Arias, Saioa Oscoz, Daniel T. Griffiths, Alice M. Luther, Majken Lindholm, Rachael A. Leah, Marie Sofie Møller, Hanif Ali, John McCafferty, Bruno Lomonte, José M. Gutiérrez, Aneesh Karatt-Vellatt
Publié dans: mAbs, 2022, ISSN 1942-0862
Éditeur: Taylor & Francis
DOI: 10.1080/19420862.2022.2085536

Advances in antibody phage display technology

Auteurs: Line Ledsgaard, AnneLjungars, Charlotte Rimbault, Christoffer V. Sørensen, Tulika Tulika, Jack Wade, Yessica Wouters, John McCafferty, Andreas H. Laustsen
Publié dans: Drug Discovery Today, 2022, ISSN 1359-6446
Éditeur: Elsevier BV
DOI: 10.1016/j.drudis.2022.05.002

Current Knowledge on Snake Dry Bites

Auteurs: Manuela B. Pucca, Cecilie Knudsen, Isadora S. Oliveira, Charlotte Rimbault, Felipe A. Cerni, Fan Hui Wen, Jacqueline Sachett, Marco A. Sartim, Andreas H. Laustsen, Wuelton M. Monteiro
Publié dans: Toxins, 2020, ISSN 2072-6651
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins12110668

Discovery of broadly‐neutralizing antibodies against brown recluse spider and Gadim scorpion sphingomyelinases using consensus toxins as antigens

Auteurs: Esperanza Rivera‐de‐Torre, Stefanos Lampadariou, Mark Møiniche, Markus F. Bohn, Seyed Mahdi Kazemi, Andreas H. Laustsen
Publié dans: Protein Science, Numéro 33, 2024, ISSN 0961-8368
Éditeur: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4901

Phage display assisted discovery of a <scp>pH</scp>‐dependent anti‐α‐cobratoxin antibody from a natural variable domain library

Auteurs: Tulika Tulika, Rasmus W. Pedersen, Charlotte Rimbault, Shirin Ahmadi, Esperanza Rivera‐de‐Torre, Monica L. Fernández‐Quintero, Johannes R. Loeffler, Markus‐Frederik Bohn, Anne Ljungars, Line Ledsgaard, Bjørn G. Voldborg, Fulgencio Ruso‐Julve, Jan Terje Andersen, Andreas H. Laustsen
Publié dans: Protein Science, Numéro 32, 2024, ISSN 0961-8368
Éditeur: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4821

Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins

Auteurs: Esperanza Rivera-de-Torre; Charlotte Rimbault; Timothy P. Jenkins; Christoffer V. Sørensen; Anna Damsbo; Natalie J. Saez; Yoan Duhoo; Celeste Menuet Hackney; Lars Ellgaard; Andreas H. Laustsen
Publié dans: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Éditeur: Frontiers Media
DOI: 10.3389/fbioe.2021.811905

Assessing developability early in the discovery process for novel biologics

Auteurs: Monica L. Fernández-Quintero, Anne Ljungars, Franz Waibl, Victor Greiff, Jan Terje Andersen, Torleif T. Gjølberg, Timothy P. Jenkins, Bjørn Gunnar Voldborg, Lise Marie Grav, Sandeep Kumar, Guy Georges, Hubert Kettenberger, Klaus R. Liedl, Peter M. Tessier, John McCafferty, Andreas H. Laustsen
Publié dans: mAbs, Numéro 15, 2024, ISSN 1942-0870
Éditeur: Landes Bioscience
DOI: 10.1080/19420862.2023.2171248

Recombinant snake antivenoms get closer to the clinic

Auteurs: Andreas H. Laustsen
Publié dans: Trends in Immunology, Numéro 45, 2024, Page(s) 225-227, ISSN 1471-4906
Éditeur: Elsevier BV
DOI: 10.1016/j.it.2024.03.001

From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research

Auteurs: Shirin Ahmadi, Melisa Benard-Valle, Kim Boddum, Fernanda C. Cardoso, Glenn F. King, Andreas Hougaard Laustsen, Anne Ljungars
Publié dans: Frontiers in Pharmacology, Numéro 14, 2023, ISSN 1663-9812
Éditeur: Frontiers Media S.A.
DOI: 10.3389/fphar.2023.1249336

Cross-reactivity trends when selecting scFv antibodies against snake toxins using a phage display-based cross-panning strategy

Auteurs: Christoffer V. Sørensen, Line Ledsgaard, Helen H. K. Wildenauer, Camilla H. Dahl, Tasja W. Ebersole, Markus-Frederik Bohn, Anne Ljungars, Timothy P. Jenkins, Andreas H. Laustsen
Publié dans: Scientific Reports, Numéro 13, 2023, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-023-37056-6

Animal Immunization, in Vitro Display Technologies, and Machine Learning for Antibody Discovery.

Auteurs: Andreas Hougaard Laustsen; Victor Greiff; Aneesh Karatt-Vellatt; Serge Muyldermans; Timothy P. Jenkins
Publié dans: Trends in Biotechnology, 2021, ISSN 1879-3096
Éditeur: Elsevier
DOI: 10.1016/j.tibtech.2021.03.003

A single-chain variable fragment selected against a conformational epitope of a recombinantly produced snake toxin using phage display

Auteurs: Charlotte Rimbault, Pelle D. Knudsen, Anna Damsbo, Kim Boddum, Hanif Ali, Celeste M. Hackney, Lars Ellgaard, Markus-Frederik Bohn, Andreas H. Laustsen
Publié dans: New Biotechnology, Numéro 76, 2023, Page(s) 23-32, ISSN 1871-6784
Éditeur: Elsevier BV
DOI: 10.1016/j.nbt.2023.04.002

The challenges with developing therapeutic monoclonal antibodies for pandemic application

Auteurs: Andreas H. Laustsen, Markus-Frederik Bohn, Anne Ljungars
Publié dans: Expert Opinion on Drug Discovery, 2021, ISSN 1746-0441
Éditeur: Informa Healthcare
DOI: 10.1080/17460441.2021.1976141

Structural and functional analyses of nematode-derived antimicrobial peptides support the occurrence of direct mechanisms of worm-microbiota interactions

Auteurs: James Rooney, Esperanza Rivera-de-Torre, Ruizhe Li, Kevin Mclean, Daniel R.G. Price, Alasdair J. Nisbet, Andreas H. Laustsen, Timothy P. Jenkins, Andreas Hofmann, Somenath Bakshi, Ashraf Zarkan, Cinzia Cantacessi
Publié dans: Computational and Structural Biotechnology Journal, Numéro 23, 2024, Page(s) 1522-1533, ISSN 2001-0370
Éditeur: Elsevier
DOI: 10.1016/j.csbj.2024.04.019

A comparative study of the performance of E. coli and K. phaffii for expressing α-cobratoxin

Auteurs: Anna Damsbo, Charlotte Rimbault, Nick J. Burlet, Anneline Vlamynck, Ida Bisbo, Selma B. Belfakir, Andreas H. Laustsen, Esperanza Rivera-de-Torre
Publié dans: Toxicon, Numéro 239, 2024, Page(s) 107613, ISSN 0041-0101
Éditeur: Pergamon Press
DOI: 10.1016/j.toxicon.2024.107613

In vivo neutralization of coral snake venoms with an oligoclonal nanobody mixture in a murine challenge model

Auteurs: Melisa Benard-Valle, Yessica Wouters, Anne Ljungars, Giang Thi Tuyet Nguyen, Shirin Ahmadi, Tasja Wainani Ebersole, Camilla Holst Dahl, Alid Guadarrama-Martínez, Frederikke Jeppesen, Helena Eriksen, Gibran Rodríguez-Barrera, Kim Boddum, Timothy Patrick Jenkins, Sara Petersen Bjørn, Sanne Schoffelen, Bjørn Gunnar Voldborg, Alejandro Alagón, Andreas Hougaard Laustsen
Publié dans: Nature Communications, Numéro 15, 2024, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-024-48539-z

Engineering of pH-dependent antigen binding properties for toxin-targeting IgG1 antibodies using light-chain shuffling

Auteurs: Tulika Tulika, Fulgencio Ruso-Julve, Shirin Ahmadi, Anne Ljungars, Esperanza Rivera-de-Torre, Jack Wade, Monica L. Fernández-Quintero, Timothy P. Jenkins, Selma B. Belfakir, Georgina M.S. Ross, Lars Boyens-Thiele, Alexander K. Buell, Siri A. Sakya, Christoffer V. Sørensen, Markus-Frederik Bohn, Line Ledsgaard, Bjørn G. Voldborg, Chiara Francavilla, Tilman Schlothauer, Bruno Lomonte, Jan Terje A
Publié dans: Structure, Numéro 32, 2024, Page(s) 1404-1418.e7, ISSN 0969-2126
Éditeur: Cell Press
DOI: 10.1016/j.str.2024.07.014

AHA: AI-guided tool for the quantification of venom-induced haemorrhage in mice

Auteurs: Timothy P. Jenkins1, William Michael Laprade, Andrés Sánchez, Tulika Tulika, Carol O’Brien, Christoffer V. Sørensen, Trenton K. Stewart, Thomas Fryer, Andreas H. Laustsen, José María Gutiérrez
Publié dans: Frontiers in Tropical Diseases, 2022, ISSN 2673-7515
Éditeur: Frontiers
DOI: 10.3389/fitd.2022.1063640

De novo designed proteins neutralize lethal snake venom toxins

Auteurs: David Baker, Susana Vazquez Torres, Melisa Benard Valle, Stephen Mackessy, Stefanie Menzies, Nicholas Casewell, Shirin Ahmadi, Nick Burlet, Edin Muratspahić, Isaac Sappington, Max Overath, Esperanza Rivera-de-Torre, Jann Ledergerber, Andreas Hougaard Laustsen, Kim Boddum, Asim Bera, Alex Kang, Evans Brackenbrough, Iara Cardoso, Edouard Crittenden, Rebecca Edge, Justin Decarreau, Robert Ragotte, A
Publié dans: Nature, 2025, ISSN 1476-4687
Éditeur: Springer Nature
DOI: 10.21203/rs.3.rs-4402792/v1

Target product profiles for pan-Africa recombinant antivenoms against neurotoxic or hemotoxic and cytotoxic snakebite envenoming

Auteurs: Andreas H. Laustsen, Melisa Benard-Valle, Abdulrazaq G. Habib, Nicholas R. Casewell, Michael Abouyannis, David G. Lalloo, Anne Ljungars
Publié dans: PLOS Neglected Tropical Diseases, Numéro 19, 2025, Page(s) e0012833, ISSN 1935-2735
Éditeur: Public Library of Sciences
DOI: 10.1371/journal.pntd.0012833

Towards better antivenoms: navigating the road to new types of snakebite envenoming therapies

Auteurs: Suthimon Thumtecho, Nick J. Burlet, Anne Ljungars, Andreas H. Laustsen
Publié dans: Journal of Venomous Animals and Toxins including Tropical Diseases, Numéro 29, 2023, ISSN 1678-9199
Éditeur: Center for the Study of Venoms and Venomous Animals (CEVAP)
DOI: 10.1590/1678-9199-jvatitd-2023-0057

Discovery and optimization of a broadly-neutralizing human monoclonal antibody against long-chain α-neurotoxins from snakes

Auteurs: Line Ledsgaard, Jack Wade, Timothy P. Jenkins, Kim Boddum, Irina Oganesyan, Julian A. Harrison, Pedro Villar, Rachael A. Leah, Renato Zenobi, Sanne Schoffelen, Bjørn Voldborg, Anne Ljungars, John McCafferty, Bruno Lomonte, José M. Gutiérrez, Andreas H. Laustsen, Aneesh Karatt-Vellatt
Publié dans: Nature Communications, Numéro 14, 2023, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-023-36393-4

Terrestrial venomous animals, the envenomings they cause, and treatment perspectives in the Middle East and North Africa.

Auteurs: Timothy P. Jenkins; Shirin Ahmadi; Mátyás A. Bittenbinder; Mátyás A. Bittenbinder; Trenton K Stewart; Dilber E Akgun; Melissa Hale; Nafiseh Nasri Nasrabadi; Nafiseh Nasri Nasrabadi; Darian S Wolff; Freek J. Vonk; Freek J. Vonk; Jeroen Kool; Andreas Hougaard Laustsen
Publié dans: PLOS Neglected Tropical Diseases, 2021, ISSN 1935-2727
Éditeur: Public Library of Science
DOI: 10.1371/journal.pntd.0009880

Monographic books (1)

Discovery of antibodies with pH-dependent binding towards snake venom toxin

Auteurs: Ej Efternavn, Tulika
Publié dans: Ej Efternavn , T 2023 , Discovery of antibodies with pH-dependent binding towards snake venom toxin . DTU Bioengineering , Kgs. Lyngby, Denmark ., Numéro 1, 2023
Éditeur: Technical University of Denmark