Periodic Reporting for period 1 - UNILIB (Universal regulatory library for any cell type) Reporting period: 2019-07-01 to 2020-12-31 Summary of the context and overall objectives of the project Problem being addressed:The lack of a reliable promoter resource has hindered the development of many synthetic biology applications, especially based on Eukaryotic chassis.Importance for society:Many application that are based on yeast can be made possible, including but not limited to various foot products, biologics, and other novel engineered living materials.General objective:To develop a promoter resource in yeast that can function reliably in any growth condition. Work performed from the beginning of the project to the end of the period covered by the report and main results achieved so far We designed an oligo library encoding 200,000 variants containing up to 3 binding sites from a list of 42 TF binding sites identified in MRG-Grammar. The library was cloned upstream to a minimal yeast synthetic promoter and characterized via SORT-Seq. The library generated a spectrum of expression levels achieving the resource goal. Progress beyond the state of the art and expected potential impact (including the socio-economic impact and the wider societal implications of the project so far) We now possess a promoter resource in yeast that heretofore did not exist. Prior to publication we expect to characterize the library in another 4-5 growth conditions, thus completing the resource. We expect the library to be widely impactful on yeast based applications. UNILIB logo
