Universal regulatory library for any cell type

Información del proyecto

UNILIB

Identificador del acuerdo de subvención: 851615

DOI

10.3030/851615

Proyecto cerrado

Fecha de la firma de la CE 6 Junio 2019

Fecha de inicio 1 Julio 2019

Fecha de finalización 31 Diciembre 2020

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 100 000,00

Aportación de la UE

€ 100 000,00

Coordinado por

TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Israel

Periodic Reporting for period 1 - UNILIB (Universal regulatory library for any cell type)

Período documentado: 2019-07-01 hasta 2020-12-31

Resumen del contexto y de los objetivos generales del proyecto

Problem being addressed:

The lack of a reliable promoter resource has hindered the development of many synthetic biology applications, especially based on Eukaryotic chassis.

Importance for society:

Many application that are based on yeast can be made possible, including but not limited to various foot products, biologics, and other novel engineered living materials.

General objective:

To develop a promoter resource in yeast that can function reliably in any growth condition.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

We designed an oligo library encoding 200,000 variants containing up to 3 binding sites from a list of 42 TF binding sites identified in MRG-Grammar. The library was cloned upstream to a minimal yeast synthetic promoter and characterized via SORT-Seq. The library generated a spectrum of expression levels achieving the resource goal.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

We now possess a promoter resource in yeast that heretofore did not exist. Prior to publication we expect to characterize the library in another 4-5 growth conditions, thus completing the resource. We expect the library to be widely impactful on yeast based applications.

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