Periodic Reporting for period 1 - UNILIB (Universal regulatory library for any cell type)
Período documentado: 2019-07-01 hasta 2020-12-31
Problem being addressed:
The lack of a reliable promoter resource has hindered the development of many synthetic biology applications, especially based on Eukaryotic chassis.
Importance for society:
Many application that are based on yeast can be made possible, including but not limited to various foot products, biologics, and other novel engineered living materials.
General objective:
To develop a promoter resource in yeast that can function reliably in any growth condition.
The lack of a reliable promoter resource has hindered the development of many synthetic biology applications, especially based on Eukaryotic chassis.
Importance for society:
Many application that are based on yeast can be made possible, including but not limited to various foot products, biologics, and other novel engineered living materials.
General objective:
To develop a promoter resource in yeast that can function reliably in any growth condition.
We designed an oligo library encoding 200,000 variants containing up to 3 binding sites from a list of 42 TF binding sites identified in MRG-Grammar. The library was cloned upstream to a minimal yeast synthetic promoter and characterized via SORT-Seq. The library generated a spectrum of expression levels achieving the resource goal.
We now possess a promoter resource in yeast that heretofore did not exist. Prior to publication we expect to characterize the library in another 4-5 growth conditions, thus completing the resource. We expect the library to be widely impactful on yeast based applications.