Universal regulatory library for any cell type

Informations projet

UNILIB

N° de convention de subvention: 851615

DOI

10.3030/851615

Projet clôturé

Date de signature de la CE 6 Juin 2019

Date de début 1 Juillet 2019

Date de fin 31 Decembre 2020

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 100 000,00

Contribution de l’UE

€ 100 000,00

Coordonné par

TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Israel

Periodic Reporting for period 1 - UNILIB (Universal regulatory library for any cell type)

Période du rapport: 2019-07-01 au 2020-12-31

Résumé du contexte et des objectifs généraux du projet

Problem being addressed:

The lack of a reliable promoter resource has hindered the development of many synthetic biology applications, especially based on Eukaryotic chassis.

Importance for society:

Many application that are based on yeast can be made possible, including but not limited to various foot products, biologics, and other novel engineered living materials.

General objective:

To develop a promoter resource in yeast that can function reliably in any growth condition.

Travail effectué depuis le début du projet jusqu’à la fin de la période considérée dans le rapport et principaux résultats atteints jusqu’à présent

We designed an oligo library encoding 200,000 variants containing up to 3 binding sites from a list of 42 TF binding sites identified in MRG-Grammar. The library was cloned upstream to a minimal yeast synthetic promoter and characterized via SORT-Seq. The library generated a spectrum of expression levels achieving the resource goal.

Progrès au-delà de l’état des connaissances et impact potentiel prévu (y compris l’impact socio-économique et les conséquences sociétales plus larges du projet jusqu’à présent)

We now possess a promoter resource in yeast that heretofore did not exist. Prior to publication we expect to characterize the library in another 4-5 growth conditions, thus completing the resource. We expect the library to be widely impactful on yeast based applications.

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