Universal regulatory library for any cell type

Informazioni relative al progetto

UNILIB

ID dell’accordo di sovvenzione: 851615

DOI

10.3030/851615

Progetto chiuso

Data della firma CE 6 Giugno 2019

Data di avvio 1 Luglio 2019

Data di completamento 31 Dicembre 2020

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 100 000,00

Contributo UE

€ 100 000,00

Coordinato da

TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Israel

Periodic Reporting for period 1 - UNILIB (Universal regulatory library for any cell type)

Periodo di rendicontazione: 2019-07-01 al 2020-12-31

Sintesi del contesto e degli obiettivi generali del progetto

Problem being addressed:

The lack of a reliable promoter resource has hindered the development of many synthetic biology applications, especially based on Eukaryotic chassis.

Importance for society:

Many application that are based on yeast can be made possible, including but not limited to various foot products, biologics, and other novel engineered living materials.

General objective:

To develop a promoter resource in yeast that can function reliably in any growth condition.

Lavoro eseguito dall’inizio del progetto fino alla fine del periodo coperto dalla relazione e principali risultati finora ottenuti

We designed an oligo library encoding 200,000 variants containing up to 3 binding sites from a list of 42 TF binding sites identified in MRG-Grammar. The library was cloned upstream to a minimal yeast synthetic promoter and characterized via SORT-Seq. The library generated a spectrum of expression levels achieving the resource goal.

Progressi oltre lo stato dell’arte e potenziale impatto previsto (incluso l’impatto socioeconomico e le implicazioni sociali più ampie del progetto fino ad ora)

We now possess a promoter resource in yeast that heretofore did not exist. Prior to publication we expect to characterize the library in another 4-5 growth conditions, thus completing the resource. We expect the library to be widely impactful on yeast based applications.

UNILIB logo